Crystal structure of the C-terminal domain of S.cerevisiae eIF5.

abstract：:The development of drug resistance has long plagued our efforts to curtail viral infections in general and influenza in particular. The problem is particularly challenging since the exact mode of resistance may be difficult to predict, without waiting for untreatable strains to evolve. Herein, a different approach is ...

journal_title：Journal of molecular biology

authors： Assa D,Alhadeff R,Krugliak M,Arkin IT

更新日期：2016-10-09 00:00:00

abstract：:The folding kinetics and thermodynamics of the isolated C-terminal domain of the ribosomal protein L9 (CTL9) have been studied as a function of pH. CTL9 is an alpha-beta protein that contains a single beta-sheet with an unusual mixed parallel, anti-parallel topology. The folding is fully reversible and two-state over ...

journal_title：Journal of molecular biology

authors： Sato S,Raleigh DP

更新日期：2002-04-26 00:00:00

abstract：:The methylation-dependent restriction endonuclease McrBC from Escherichia coli K12 cleaves DNA containing two R(m)C dinucleotides separated by about 40 to 2000 base-pairs. McrBC is unique in that cleavage is totally dependent on GTP hydrolysis. McrB is the GTP binding and hydrolyzing subunit, whereas MrC stimulates it...

journal_title：Journal of molecular biology

authors： Pieper U,Schweitzer T,Groll DH,Gast FU,Pingoud A

更新日期：1999-09-24 00:00:00

abstract：:The vertebrate striated muscle protein titin is thought to play a critical rôle in myofibril assembly and passive tension. The recently determined complete primary structure of titin revealed a modular architecture that opens the way to a structural characterisation and the understanding of essential properties of thi...

journal_title：Journal of molecular biology

authors： Politou AS,Gautel M,Improta S,Vangelista L,Pastore A

更新日期：1996-02-02 00:00:00

abstract：:Ligands that activate the nuclear receptor retinoid X receptor (RXR) display potent anticarcinogenic activities, but the mechanisms by which these compounds inhibit carcinoma cell growth are poorly understood. While RXR can regulate gene expression due to its intrinsic ligand-activated transcription function, this rec...

journal_title：Journal of molecular biology

authors： Yasmin R,Kannan-Thulasiraman P,Kagechika H,Dawson MI,Noy N

更新日期：2010-04-16 00:00:00

abstract：:Genetic circuits, composed of complex networks of interacting molecular machines, enable living systems to sense their dynamic environments, perform computation on the inputs, and formulate appropriate outputs. By rewiring and expanding these circuits with novel parts and modules, synthetic biologists have adapted liv...

journal_title：Journal of molecular biology

authors： Ma KC,Perli SD,Lu TK

更新日期：2016-02-27 00:00:00

abstract：:The structure of the Escherichia coli gene coding for the metabolically stable 4.5 S RNA has been determined by cloning and DNA sequence analysis. Results from Southern hybridization assays carried out prior to cloning show the 4.5 S DNA to be limited to a single locus in the E. coli K12 genome. A 5.4 X 10(3) base DNA...

journal_title：Journal of molecular biology

authors： Hsu LM,Zagorski J,Fournier MJ

更新日期：1984-09-25 00:00:00

abstract：:Biosynthesis of NAD(P) in bacteria occurs either de novo or through one of the salvage pathways that converge at the point where the reaction of nicotinate mononucleotide (NaMN) with ATP is coupled to the formation of nicotinate adenine dinucleotide (NaAD) and inorganic pyrophosphate. This reaction is catalyzed by nic...

journal_title：Journal of molecular biology

authors： Sershon VC,Santarsiero BD,Mesecar AD

更新日期：2009-01-23 00:00:00

abstract：:Two-dimensional nuclear Overhauser effect (2D NOE) spectra have been used as the experimental basis for determining the solution structure of the duplex [d(GTATATAC)]2 employing restrained molecular dynamics (rMD) simulations. The MARDIGRAS algorithm has been employed to construct a set of 233 interproton distance con...

journal_title：Journal of molecular biology

authors： Schmitz U,Pearlman DA,James TL

更新日期：1991-09-05 00:00:00

abstract：:RT6 is a glycosyl phosphatidylinositol-anchored cell membrane protein, whose expression is restricted to peripheral T cells and intraepithelial lymphocytes. It has attracted interest as a T cell differentiation marker and activation antigen in rats. The only known protein to which RT6 shows significant homology is a r...

journal_title：Journal of molecular biology

authors： Haag F,Koch-Nolte F,Kühl M,Lorenzen S,Thiele HG

更新日期：1994-10-28 00:00:00

abstract：:Pre-steady-state chemical quenched-flow techniques were used to study DNA unwinding catalyzed by Escherichia coli UvrD helicase (helicase II), a member of the SF1 helicase superfamily. Single turnover experiments, with respect to unwinding of a DNA oligonucleotide, were used to examine the DNA substrate and UvrD conce...

journal_title：Journal of molecular biology

authors： Ali JA,Maluf NK,Lohman TM

更新日期：1999-11-05 00:00:00

abstract：:The properties have been determined of a recently identified form of bovine pancreatic trypsin inhibitor, with only two of the three disulphide bonds of the native protein, but possessing a native-like conformation. The kinetics of refolding of the reduced inhibitor were re-measured to elucidate the kinetic role in fo...

journal_title：Journal of molecular biology

authors： Creighton TE,Goldenberg DP

更新日期：1984-11-05 00:00:00

abstract：:Enterococcus faecalis, a ubiquitous member of the healthy human gut microbiota, is also a common opportunistic pathogen and leading cause of nosocomial infections. This tenacious microbe is well adapted to infect and persist in multiple niches within the mammalian host and can rapidly tune its metabolism to respond to...

journal_title：Journal of molecular biology

authors： Kao PHN,Kline KA

更新日期：2019-07-26 00:00:00

abstract：:Human islet amyloid polypeptide (hIAPP), which is considered the primary culprit for beta-cell loss in type 2 diabetes mellitus patients, is synthesized in beta-cells of the pancreas from its precursor pro-islet amyloid polypeptide (proIAPP), which may be important in early intracellular amyloid formation as well. We ...

journal_title：Journal of molecular biology

authors： Jha S,Sellin D,Seidel R,Winter R

更新日期：2009-06-26 00:00:00

abstract：:As the receptor on the platelet surface for von Willebrand factor, glycoprotein (GP) Ib-IX complex is critically involved in hemostasis and thrombosis. How the complex is assembled from GP Ibalpha, GP Ib beta and GP IX subunits, all of which are type I transmembrane proteins, is not entirely clear. Genetic and mutatio...

journal_title：Journal of molecular biology

authors： Luo SZ,Li R

更新日期：2008-10-03 00:00:00

abstract：:Replication of plasmid ColE1 is regulated by a plasmid-specified small RNA (RNA I). RNA I binds to the precursor (RNA II) of the primer for DNA synthesis and inhibits primer formation. The process of binding of RNA I to RNA II that results in formation of a stably bound complex consists of a series of reactions formin...

journal_title：Journal of molecular biology

authors： Tomizawa J

更新日期：1990-04-20 00:00:00

abstract：:In many tailed bacteriophages, DNA packaging is initiated by recognition and cleavage of a specific sequence pac by the small (TerS) and large (TerL) terminase subunits. It was previously shown that the SPP1 pac region has two sequences where TerS binds (pacR and pacL), flanking the segment where TerL cleaves the SPP1...

journal_title：Journal of molecular biology

authors： Djacem K,Tavares P,Oliveira L

更新日期：2017-05-05 00:00:00

abstract：:To define the structural basis for cofactor binding to membrane proteins, we introduce a manageable model system, which allows us, for the first time, to study the influence of individual transmembrane helices and of single amino acid residues on the assembly of a transmembrane cytochrome. In vivo as well as in vitro ...

journal_title：Journal of molecular biology

authors： Prodöhl A,Volkmer T,Finger C,Schneider D

更新日期：2005-07-22 00:00:00

abstract：:Antibiotic chloramphenicol (CHL) binds with a moderate affinity at the peptidyl transferase center of the bacterial ribosome and inhibits peptide bond formation. As an approach for modifying and potentially improving properties of this inhibitor, we explored ribosome binding and inhibitory activity of a number of amin...

journal_title：Journal of molecular biology

authors： Tereshchenkov AG,Dobosz-Bartoszek M,Osterman IA,Marks J,Sergeeva VA,Kasatsky P,Komarova ES,Stavrianidi AN,Rodin IA,Konevega AL,Sergiev PV,Sumbatyan NV,Mankin AS,Bogdanov AA,Polikanov YS

更新日期：2018-03-16 00:00:00

abstract：:When theoretical methods are used to predict the properties of a given system, such as the effects of the substitution of a specific amino acid on the activity or stability of a protein as a whole, the accuracy of the prediction is directly dependent on the validity of the underlying model. A common error, however, is...

journal_title：Journal of molecular biology

authors： van Gunsteren WF,Mark AE

更新日期：1992-09-20 00:00:00

abstract：:Crystals of NADPH:FMN oxidoreductase from Vibrio harveyi have been obtained and characterized by X-ray diffraction. This enzyme plays a role in the generation of light in luminescent bacteria by providing reduced FMN to luciferase. Large, high quality crystals were grown using polyethylene glycol 6000 at pH 7.0. They ...

journal_title：Journal of molecular biology

authors： Tanner J,Lei B,Liu M,Tu SC,Krause KL

更新日期：1994-08-12 00:00:00

abstract：:Along with the discovery of tumor-driven inflammatory pathways, there has been a considerable progress over the past 10years in understanding the mechanisms leading to cancer immunosurveillance and immunoediting. Several regulatory pathways, typically involved in immune cell homeostasis, are co-opted by cancer cells t...

journal_title：Journal of molecular biology

authors： Rabinovich GA,Conejo-García JR

更新日期：2016-08-14 00:00:00

abstract：:The roles of the residues in the catalytic trio Glu212-Asp214-Glu217 in cellobiohydrolase I (CBHI) from Trichoderma reesei have been investigated by changing these residues to their isosteric amide counterparts. Three mutants, E212Q, D214N and E217Q, were constructed and expressed in T. reesei. All three point mutatio...

journal_title：Journal of molecular biology

authors： Ståhlberg J,Divne C,Koivula A,Piens K,Claeyssens M,Teeri TT,Jones TA

更新日期：1996-11-29 00:00:00

abstract：:p50 protein is a member of the Y-box binding transcription factor family and is a counterpart of YB-1 protein. The generic microchip was used to analyze the sequence specificity of p50 binding to single (ss) and double-stranded (ds) oligodeoxyribonucleotides. The generic microchip contained 4,096 single-stranded octad...

journal_title：Journal of molecular biology

authors： Zasedateleva OA,Krylov AS,Prokopenko DV,Skabkin MA,Ovchinnikov LP,Kolchinsky A,Mirzabekov AD

更新日期：2002-11-15 00:00:00

abstract：:Crystals of an inhibitor of trypsin and tissue plasminogen activator from seeds of the legume Erythrina caffra have been obtained by vapour diffusion. The crystals belong to the hexagonal space group P6(1)22 (or its enantiomorph P6(5)22) with cell parameters 73.4 A and 143.0 A. There is one molecule in the asymmetric ...

journal_title：Journal of molecular biology

authors： Onesti S,Lloyd LF,Maeder D,Mistry A,Brick P

更新日期：1989-11-05 00:00:00

abstract：:The crystal and molecular structure of a dextran hydrate has been determined through combined electron and X-ray diffraction analysis, aided by stereochemical model refinement. A total of 65 hk0 electron diffraction intensities were measured on frozen single crystals held at the temperature of liquid nitrogen, to a re...

journal_title：Journal of molecular biology

authors： Guizard C,Chanzy H,Sarko A

更新日期：1985-06-05 00:00:00

abstract：:Prompted by the close relationship between tyrosine recombinases and type IB topoisomerases we have investigated the ability of human topoisomerase I to resolve the typical intermediate of recombinase catalysis, the Holliday junction. We demonstrate that human topoisomerase I catalyzes unidirectional resolution of a s...

journal_title：Journal of molecular biology

authors： Hede MS,Petersen RL,Frøhlich RF,Krüger D,Andersen FF,Andersen AH,Knudsen BR

更新日期：2007-01-26 00:00:00

abstract：:Bacteriorhodopsin is the smallest autonomous light-driven proton pump. Proposals as to how it achieves the directionality of its trans-membrane proton transport fall into two categories: accessibility-switch models in which proton transfer pathways in different parts of the molecule are opened and closed during the ph...

journal_title：Journal of molecular biology

authors： Onufriev A,Smondyrev A,Bashford D

更新日期：2003-10-03 00:00:00

abstract：:The control of DNA replication initiation in the plasmid ColE1 is mediated by RNAI, a 108 nucleotide plasmid-encoded RNA that is entirely complementary to the 5'-terminal region of the replication primer RNA. RNAI acts in trans to inhibit primer maturation. Previously, we constructed a plasmid in which the ColE1 RNAI ...

journal_title：Journal of molecular biology

authors： Dooley TP,Tamm J,Polisky B

更新日期：1985-11-05 00:00:00

abstract：:3-Methylitaconate-Delta-isomerase (Mii) participates in the nicotinate fermentation pathway of the anaerobic soil bacterium Eubacterium barkeri (order Clostridiales) by catalyzing the reversible conversion of (R)-3-methylitaconate (2-methylene-3-methylsuccinate) to 2,3-dimethylmaleate. The enzyme is also able to catal...

journal_title：Journal of molecular biology

authors： Velarde M,Macieira S,Hilberg M,Bröker G,Tu SM,Golding BT,Pierik AJ,Buckel W,Messerschmidt A

更新日期：2009-08-21 00:00:00