当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Functional reconstitution of purified human Hv1 H+ channels.

Functional reconstitution of purified human Hv1 H+ channels.

Abstract：

:Voltage-dependent H(+) (Hv) channels mediate proton conduction into and out of cells under the control of membrane voltage. Hv channels are unusual compared to voltage-dependent K(+), Na(+), and Ca(2+) channels in that Hv channel genes encode a voltage sensor domain (VSD) without a pore domain. The H(+) currents observed when Hv channels are expressed heterologously suggest that the VSD itself provides the pathway for proton conduction. In order to exclude the possibility that the Hv channel VSD assembles with an as yet unknown protein in the cell membrane as a requirement for H(+) conduction, we have purified Hv channels to homogeneity and reconstituted them into synthetic lipid liposomes. The Hv channel VSD by itself supports H(+) flux.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Lee SY,Letts JA,MacKinnon R

doi

10.1016/j.jmb.2009.02.034

subject

Has Abstract

pub_date

2009-04-17 00:00:00

pages

1055-60

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(09)00204-6

journal_volume

387

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of Bacillus polymyxa.

    abstract：:The enzyme encoded by the bgIA gene of Bacillus polymyxa, a type I beta-glucosidase belonging to family I of glycosyl hydrolases, has been purified to homogeneity from an Escherichia coli culture which overexpressed the gene, and crystallized. The crystals, which diffract to 3.0 A resolution, belong to the orthorhombi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1441

    authors： Sanz-Aparicio J,Romero A,Martinez-Ripoll M,Madarro A,Flors A,Polaina J

    更新日期：1994-07-15 00:00:00

  • Peptide models of local and long-range interactions in the molten globule state of human alpha-lactalbumin.

    abstract：:alpha-Lactalbumin, a small calcium-binding protein, forms an equilibrium molten globule state under a variety of conditions. A set of four peptides designed to probe the role of local interactions and the role of potential long-range interactions in stabilizing the molten globule of alpha-lactalbumin has been prepared...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2099

    authors： Demarest SJ,Fairman R,Raleigh DP

    更新日期：1998-01-01 00:00:00

  • Structure of complexes between a major protein of heterogeneous nuclear ribonucleoprotein particles and polyribonucleotides.

    abstract：:We have investigated the structure of complexes formed between a series of poly(A)n (n = 30 to 480) and HD40 (helix-destabilizing protein, molecular weight of 40,000), the major protein component of 30 S heterogeneous nuclear ribonucleoprotein particles (hnRNP) from the brine shrimp Artemia salina. Protein HD40 is sim...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(83)90039-6

    authors： Thomas JO,Glowacka SK,Szer W

    更新日期：1983-12-25 00:00:00

  • The 2.0 A crystal structure of Scapharca tetrameric hemoglobin: cooperative dimers within an allosteric tetramer.

    abstract：:The crystal structure of the allosteric tetrameric hemoglobin from Scapharca inaequivalvis (HbII) has been determined in the carbonmonoxy liganded state using a combination of anomalous scattering and molecular replacement. The molecular model has been refined at 2.0 A resolution to a conventional R-factor of 0.173 an...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0543

    authors： Royer WE Jr,Heard KS,Harrington DJ,Chiancone E

    更新日期：1995-10-13 00:00:00

  • All-D peptides recognized by an anti-carbohydrate antibody identified from a positional scanning library.

    abstract：:Monoclonal antibodies recognize antigens with high affinity and specificity, but the structural basis for molecular mimicry remains unclear. It is often assumed that cross-reactive antigens share some structural similarity that is specifically recognized by a monoclonal antibody. Recent studies using combinatorial lib...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2137

    authors： Pinilla C,Appel JR,Campbell GD,Buencamino J,Benkirane N,Muller S,Greenspan NS

    更新日期：1998-11-13 00:00:00

  • Common core structure of amyloid fibrils by synchrotron X-ray diffraction.

    abstract：:Tissue deposition of normally soluble proteins as insoluble amyloid fibrils is associated with serious diseases including the systemic amyloidoses, maturity onset diabetes, Alzheimer's disease and transmissible spongiform encephalopathy. Although the precursor proteins in different diseases do not share sequence homol...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1348

    authors： Sunde M,Serpell LC,Bartlam M,Fraser PE,Pepys MB,Blake CC

    更新日期：1997-10-31 00:00:00

  • Removal of a terminator structure by RNA processing regulates int gene expression.

    abstract：:The int gene of phage lambda encodes a protein involved in site-specific recombination. Its expression is regulated differentially during successive phases of the lambda infective cycle. The gene is transcribed early after infection from one promoter, pL, and later from a second promoter pI. Each transcription event r...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90381-4

    authors： Schmeissner U,McKenney K,Rosenberg M,Court D

    更新日期：1984-06-15 00:00:00

  • Coliphage P2 late control gene ogr. DNA sequence and product identification.

    abstract：:The bacteriophage P2 late control gene ogr was cloned and precisely localized by deletion analysis in vitro. The DNA sequence of the ogr gene containing the ogr1 mutation was determined. The sequence translates into a basic protein of a molecular weight of 8300. Plasmids overproducing the ogr gene product were constru...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(86)90170-1

    authors： Birkeland NK,Lindquist BH

    更新日期：1986-04-05 00:00:00

  • DNA-drug interactions. The crystal structures of d(TGTACA) and d(TGATCA) complexed with daunomycin.

    abstract：:The anticancer drug daunomycin has been co-crystallized with the hexanucleotide duplex sequences d(TGTACA) and d(TGATCA) and single crystal X-ray diffraction studies of these two complexes have been carried out. Structure solution of the d(TGTACA) and d(TGATCA) complexes to 1.6 and 1.7 Angstrom resolution, respectivel...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90203-i

    authors： Nunn CM,Van Meervelt L,Zhang SD,Moore MH,Kennard O

    更新日期：1991-11-20 00:00:00

  • Crystallization and preliminary X-ray data for the A-isozyme of O-acetylserine sulfhydrylase from Salmonella typhimurium.

    abstract：:The A-isozyme of O-acetylserine sulfhydrylase, a pyridoxal phosphate-dependent enzyme isolated from Salmonella typhimurium catalyzes the synthesis of L-cysteine from O-acetyl-L-serine and sulfide. The pyridoxal form of the enzyme has been crystallized in two different forms. One form is in the orthorhombic space group...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1358

    authors： Rao GS,Mottonen J,Goldsmith EJ,Cook PF

    更新日期：1993-06-20 00:00:00

  • Nuclear magnetic resonance studies of the internal dynamics in Apo, (Cd2+)1 and (Ca2+)2 calbindin D9k. The rates of amide proton exchange with solvent.

    abstract：:The backbone dynamics of the EF-hand Ca(2+)-binding protein, calbindin D9k, has been investigated in the apo, (Cd2+)1 and (Ca2+)2 states by measuring the rate constants for amide proton exchange with solvent. 15N-1H correlation spectroscopy was utilized to follow direct 1H-->2H exchange of the slowly exchanging amide ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90524-n

    authors： Skelton NJ,Kördel J,Akke M,Chazin WJ

    更新日期：1992-10-20 00:00:00

  • Identification of protein interaction partners and protein-protein interaction sites.

    abstract：:Rigid-body docking has become quite successful in predicting the correct conformations of binary protein complexes, at least when the constituent proteins do not undergo large conformational changes upon binding. However, determining whether two given proteins interact is a more difficult problem. Successful docking p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.08.002

    authors： Sacquin-Mora S,Carbone A,Lavery R

    更新日期：2008-10-24 00:00:00

  • The alternatively folded state of the antibody C(H)3 domain.

    abstract：:The C(H)3 domain of antibodies is characterized by two antiparallel beta-sheets forming a disulfide-linked sandwich-like structure. At acidic pH values and low ionic strength, C(H)3 becomes completely unfolded. The addition of salt transforms the acid-unfolded protein into an alternatively folded state exhibiting a ch...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4707

    authors： Thies MJ,Kammermeier R,Richter K,Buchner J

    更新日期：2001-06-22 00:00:00

  • Protruding domain of tomato bushy stunt virus coat protein is a hitherto unrecognized class of jellyroll conformation.

    abstract：:The capsid protein of tomato bushy stunt virus (TBSV) has two antiparallel beta-sheet domains with the so-called jellyroll conformation. Contrary to previous analyses, we note that these domains are non-superimposable topologies. The TBSV shell (S) domain topology is common to many other proteins but the protruding (P...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90298-Z

    authors： Gibson TJ,Argos P

    更新日期：1990-03-05 00:00:00

  • Limitation of ribosomal protein L11 availability in vivo affects translation termination.

    abstract：:Historically referred to as "the GTPase center", the L11 binding region (L11BR) of Escherichia coli 23 S rRNA is a highly conserved structure that has been implicated in several essential functions during protein synthesis. Here, in vivo expression of an RNA fragment containing that structure was found to affect trans...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00304-2

    authors： Van Dyke N,Xu W,Murgola EJ

    更新日期：2002-05-31 00:00:00

  • Local folding coupled to RNA binding in the yeast ribosomal protein L30.

    abstract：:The ribosomal protein L30 from yeast Saccharomyces cerevisiae auto-regulates its own synthesis by binding to a structural element in both its pre-mRNA and its mRNA. The three-dimensional structures of L30 in the free (f L30) and the pre-mRNA bound (b L30) forms have been solved by nuclear magnetic resonance spectrosco...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3044

    authors： Mao H,Williamson JR

    更新日期：1999-09-17 00:00:00

  • Mutational analysis of target base flipping by the EcoRV adenine-N6 DNA methyltransferase.

    abstract：:DNA methyltransferases flip their target base out of the DNA helix. Here, we have investigated base flipping by wild-type EcoRV DNA methyltransferase (M.EcoRV) and five M.EcoRV variants (D193A, Y196A, S229A, W231R and Y258A). These variants bind to DNA and S-adenosylmethionine but have a severely reduced catalytic eff...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2389

    authors： Jeltsch A,Roth M,Friedrich T

    更新日期：1999-01-22 00:00:00

  • Structure of the Drosophila projectin protein: isoforms and implication for projectin filament assembly.

    abstract：:The protein composition of the various muscle types in Drosophila melanogaster has been studied quite thoroughly and the analysis has revealed many differences involving the usage of muscle specific isoforms of a given protein, as well as the presence of proteins restricted to one muscle type. Drosophila projectin, th...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1756

    authors： Daley J,Southgate R,Ayme-Southgate A

    更新日期：1998-05-29 00:00:00

  • The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1.

    abstract：:Protein unfolding occurs at both low and high temperatures, although in most cases, only the high-temperature transition can be experimentally studied. A pressing question is how much the low- and high-temperature denatured states, although thermodynamically equivalent, are structurally and kinetically similar. We hav...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.02.002

    authors： Adrover M,Martorell G,Martin SR,Urosev D,Konarev PV,Svergun DI,Daura X,Temussi P,Pastore A

    更新日期：2012-04-13 00:00:00

  • Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern.

    abstract：:Antimycin A (antimycin), one of the first known and most potent inhibitors of the mitochondrial respiratory chain, binds to the quinone reduction site of the cytochrome bc1 complex. Structure-activity relationship studies have shown that the N-formylamino-salicyl-amide group is responsible for most of the binding spec...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.05.053

    authors： Huang LS,Cobessi D,Tung EY,Berry EA

    更新日期：2005-08-19 00:00:00

  • Local and transmitted conformational changes on complexation of an anti-sweetener Fab.

    abstract：:Crystal structures of an Fab (NC6.8) from a murine IgG2b(kappa) antibody and its complex with a sweet-tasting, N-,N'-,N"-trisubstituted guanidine compound (NC174) have been determined by X-ray analysis. Both crystal forms are produced by a microseeding technique in polyethylene glycol (PEG) 8000 but the habits and spa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1133

    authors： Guddat LW,Shan L,Anchin JM,Linthicum DS,Edmundson AB

    更新日期：1994-02-11 00:00:00

  • How accurately and precisely can RNA structure be determined by NMR?

    abstract：:The great diversity of RNA biological functions has led to widespread interest in RNA structure. Advances in synthetic and spectroscopic techniques have recently allowed the extension of NMR methods of structure determination to RNA structures of significant size and increased biological significance. However, it has ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0855

    authors： Allain FH,Varani G

    更新日期：1997-03-28 00:00:00

  • Autogenous regulation of transcription termination factor Rho.

    abstract：:We present evidence that the transcription termination factor Rho is autogenously regulated in Escherichia coli. The steady-state level of Rho is increased approximately tenfold in rho mutant cells. In the rho+ revertants, the content of Rho is similar to the wild-type level. A rho-/rho+ merodiploid produces equimolar...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(85)90236-0

    authors： Barik S,Bhattacharya P,Das A

    更新日期：1985-04-20 00:00:00

  • Integrated Use of Biochemical, Native Mass Spectrometry, Computational, and Genome-Editing Methods to Elucidate the Mechanism of a Salmonella deglycase.

    abstract：:Salmonellais a foodborne pathogen that causes annually millions of cases of salmonellosis globally, yet Salmonella-specific antibacterials are not available. During inflammation, Salmonella utilizes the Amadori compound fructose-asparagine (F-Asn) as a nutrient through the successive action of three enzymes, including...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.08.017

    authors： Sengupta A,Wu J,Seffernick JT,Sabag-Daigle A,Thomsen N,Chen TH,Capua AD,Bell CE,Ahmer BMM,Lindert S,Wysocki VH,Gopalan V

    更新日期：2019-11-08 00:00:00

  • Cation transport: an example of structural based selectivity.

    abstract：:Through the high-resolution structure of the gramicidin A channel in lamellar phase lipids and the characterization of specific ion peptide interactions, fundamental principles for ion channel selectivity and conductance efficiency are illustrated with atomic resolution detail. Delocalized cation binding in the first ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2434

    authors： Tian F,Cross TA

    更新日期：1999-02-05 00:00:00

  • The Function of Tafazzin, a Mitochondrial Phospholipid-Lysophospholipid Acyltransferase.

    abstract：:Tafazzin is a mitochondrial enzyme that exchanges fatty acids between phospholipids by phospholipid-lysophospholipid transacylation. The reaction alters the molecular species composition and, as a result, the physical properties of lipids. In vivo, the most important substrate of tafazzin is the mitochondria-specific ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2020.03.026

    authors： Schlame M,Xu Y

    更新日期：2020-08-21 00:00:00

  • Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding.

    abstract：:The capsid (CA) protein plays crucial roles in HIV infection and replication, essential to viral maturation. The absence of high-resolution structural data on unassembled CA hinders the development of antivirals effective in inhibiting assembly. Unlike enzymes that have targetable, functional substrate-binding sites, ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.11.027

    authors： Du S,Betts L,Yang R,Shi H,Concel J,Ahn J,Aiken C,Zhang P,Yeh JI

    更新日期：2011-02-25 00:00:00

  • Two conformations of RNA polymerase II revealed by electron crystallography.

    abstract：:A new two-dimensional crystal form of yeast RNA polymerase II was obtained in which the conformation of the enzyme appears "open", allowing entry of DNA, as required for the initiation of transcription. By contrast, a previous crystal form contained the enzyme in a "closed" conformation, appropriate for retention of D...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1273

    authors： Asturias FJ,Meredith GD,Poglitsch CL,Kornberg RD

    更新日期：1997-10-03 00:00:00

  • Atomic environment energies in proteins defined from statistics of accessible and contact surface areas.

    abstract：:Atomic contact potentials are derived by statistical analysis of atomic surface contact areas versus atom type in a database of non-homologous protein structures. The atomic environment is characterized by the surface area accessible to solvent and the surface of contacts with polar and non-polar atoms. Four types of ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1006/jmbi.1995.0328

    authors： Delarue M,Koehl P

    更新日期：1995-06-09 00:00:00

  • A novel allosteric mechanism in haemoglobin. Structure of bovine deoxyhaemoglobin, absence of specific chloride-binding sites and origin of the chloride-linked Bohr effect in bovine and human haemoglobin.

    abstract：:The structure of bovine deoxyhaemoglobin has been determined at 2.2 A resolution and refined to an R-factor of 0.193 for all 32,583 reflections, and a free R-factor of 0.249 for 1527 reflections excluded from the refinement. The structure shows no significant differences between the alpha-carbon positions of bovine an...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1530

    authors： Perutz MF,Fermi G,Poyart C,Pagnier J,Kister J

    更新日期：1993-10-05 00:00:00