Abstract：
:The great diversity of RNA biological functions has led to widespread interest in RNA structure. Advances in synthetic and spectroscopic techniques have recently allowed the extension of NMR methods of structure determination to RNA structures of significant size and increased biological significance. However, it has not yet been established how accurately and precisely RNA structure can be determined by NMR. The extensive simulations presented here establish credible limits on accuracy and precision of NMR-derived RNA structures and provide quantitative calibrations to evaluate new structures. Synthetic sets of NMR constraints were generated from a crystallographically-derived ribozyme structure. The target structure was then redetermined using approximations and computational protocols derived from our experimental work. The results demonstrate that the structure of RNA molecules of at least 15,000 Da can be determined with precision and accuracy of 1 to 1.5 A, comparable to values obtained for proteins of similar size. Most encouragingly, it is shown that larger, globular and biologically more important RNA structures can be determined with significantly better accuracy and precision than smaller, elongated structures investigated until now.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Allain FH,Varani Gdoi
10.1006/jmbi.1996.0855subject
Has Abstractpub_date
1997-03-28 00:00:00pages
338-51issue
2eissn
0022-2836issn
1089-8638pii
S0022-2836(96)90855-4journal_volume
267pub_type
杂志文章abstract：:The basic leucine zipper domain of the yeast transcription factor GCN4 consists of a C-terminal leucine zipper and an N-terminal basic DNA-binding region that achieves a stable structure only after association with DNA. Backbone dynamics of a peptide encompassing the basic and leucine zipper bZip domain (residues 226-...
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doi：10.1006/jmbi.1998.2429
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1993.1358
更新日期：1993-06-20 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/s0022-2836(95)80060-3
更新日期：1995-04-28 00:00:00
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journal_title：Journal of molecular biology
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更新日期：2000-10-20 00:00:00
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更新日期：2007-04-06 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1993.1264
更新日期：1993-05-05 00:00:00
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更新日期：2007-03-23 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1997.1599
更新日期：1998-03-27 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1995.0291
更新日期：1995-06-02 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1993.1163
更新日期：1993-03-20 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1998.2087
更新日期：1998-09-25 00:00:00
abstract：:The ribosomal RNA multigene family in the fungus fly Sciara coprophila contains a total of only 65 to 70 repeat units. We explored the types and frequencies of variant repeats in this small multigene family by characterizing different cloned rDNA variants from Sciara. Although we did not observe any intergenic spacer ...
journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/0022-2836(89)90286-6
更新日期：1989-11-05 00:00:00
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更新日期：2010-07-02 00:00:00
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更新日期：1994-01-28 00:00:00
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更新日期：2006-08-18 00:00:00
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更新日期：2010-02-19 00:00:00
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journal_title：Journal of molecular biology
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更新日期：1999-09-17 00:00:00
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更新日期：2014-05-29 00:00:00
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更新日期：2003-07-25 00:00:00
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pub_type： 杂志文章
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更新日期：2008-09-12 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1999-03-19 00:00:00
