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Purification and crystallization of fumarase C from Escherichia coli.

Abstract：

:Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C222(1) form was obtained in citrate at pH 5.3 and an orthorhombic I222 form was obtained in 3-(4-morpholino)-propanesulfonic acid (pH 7.5). Complete native data sets have been collected on both crystal forms: the C222(1) form is complete to 2.10 A and the I222 form is complete to 2.20 A.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Weaver TM,Levitt DG,Banaszak LJ

doi

10.1006/jmbi.1993.1264

subject

Has Abstract

pub_date

1993-05-05 00:00:00

pages

141-4

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)71264-7

journal_volume

231

pub_type

杂志文章

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