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Ultrasonic absorption in tobacco mosaic virus and its protein aggregates.

Abstract：

:The structural fluctuations specific to self-assembled biological systems have been investigated further with ultrasonic techniques by using two strains of tobacco mosaic virus (TMV), as well as the helical aggregate of the common strain protein and subassemblies of it. We confirmed our earlier conclusion that protein assemblies exhibit specific structural fluctuations detected in ultrasonic experiments. As in spherical viruses, the fluctuations exhibited by the protein aggregates having a quaternary structure similar to that of the virion were modified in the virus by interaction with the RNA strand. It is unlikely that the origin for the observed effect is due either to: (1) the difference in local mobility of the segment 89 to 113 of the polypeptide chain in TMV and in the helical aggregate on the one hand, and in smaller aggregates, on the other hand; or (2) a local fluctuation associated with proton transfer reactions or ion-pair interactions. The most remarkable feature in the TMV system is the fact that the two-ring disk showed no excess of ultrasonic absorption with respect to the A-protein oligomer, while a large increase of ultrasonic absorption was observed in the rod-like aggregate that had undergone the disk-helix transition.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Michels B,Dormoy Y,Cerf R,Schulz JA,Witz J

doi

10.1016/0022-2836(85)90328-6

subject

Has Abstract

pub_date

1985-01-05 00:00:00

pages

103-10

issue

1

eissn

0022-2836

issn

1089-8638

pii

0022-2836(85)90328-6

journal_volume

181

pub_type

杂志文章

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