Isolation, crystallization and preliminary diffraction analyses of human pancreatic alpha-amylase.

abstract：:Single crystals of a multienzyme complex isolated from spinach leaves, and containing RUBISCO bound to the substrate RuBP have been grown and characterized. The crystals belong to the orthorhombic space group P2(1)2(1)2 with a = 173 A, b = 134 A and c = 112 A, and contain two enzyme complex molecules in the unit cell....

journal_title：Journal of molecular biology

authors： Hosur MV,Sainis JK,Kannan KK

更新日期：1993-12-20 00:00:00

abstract：:The ribonuclease inhibitor protein (RI) binds to members of the bovine pancreatic ribonuclease (RNase A) superfamily with an affinity in the femtomolar range. Here, we report on structural and energetic aspects of the interaction between human RI (hRI) and human pancreatic ribonuclease (RNase 1). The structure of the ...

journal_title：Journal of molecular biology

authors： Johnson RJ,McCoy JG,Bingman CA,Phillips GN Jr,Raines RT

更新日期：2007-04-27 00:00:00

abstract：:Previous work used hydrogen exchange (HX) experiments in kinetic and equilibrium modes to study the reversible unfolding and refolding of cytochrome c (Cyt c) under native conditions. Accumulated results now show that Cyt c is composed of five individually cooperative folding units, called foldons, which unfold and re...

journal_title：Journal of molecular biology

authors： Krishna MM,Maity H,Rumbley JN,Lin Y,Englander SW

更新日期：2006-06-23 00:00:00

abstract：:Elastomeric proteins are molecular springs found not only in a variety of biological machines and tissues, but also in biomaterials of superb mechanical properties. Regulating the mechanical stability of elastomeric proteins is not only important for a range of biological processes, but also critical for the use of en...

journal_title：Journal of molecular biology

authors： Cao Y,Yoo T,Zhuang S,Li H

更新日期：2008-05-16 00:00:00

abstract：:There are at least three different kinds of short interspersed repetitive elements in salmonid genomes. Of these, members of the HpaI family are found to be most widely distributed in salmonid species. The HpaI family is present with high copy numbers in all members of the subfamily Salmoninae, such as salmon, trout a...

journal_title：Journal of molecular biology

authors： Kido Y,Himberg M,Takasaki N,Okada N

更新日期：1994-09-02 00:00:00

abstract：:Low molecular weight protein tyrosine phosphatase (LMW-PTP) dimerizes in the phosphate-bound state in solution with a dissociation constant of K(d)=1.5(+/-0.1)mM and an off-rate on the order of 10(4)s(-1). 1H and 15N NMR chemical shifts identify the dimer interface, which is in excellent agreement with that observed i...

journal_title：Journal of molecular biology

authors： Akerud T,Thulin E,Van Etten RL,Akke M

更新日期：2002-09-06 00:00:00

abstract：:We have previously reported the structures of the native holo and substrate-bound forms of LL-diaminopimelate aminotransferase from Arabidopsis thaliana (AtDAP-AT). Here, we report the crystal and molecular structures of the LL-diaminopimelate aminotransferase from Chlamydia trachomatis (CtDAP-AT) in the apo-form and ...

journal_title：Journal of molecular biology

authors： Watanabe N,Clay MD,van Belkum MJ,Fan C,Vederas JC,James MN

更新日期：2011-08-19 00:00:00

abstract：:Stochastic fluctuations, termed "noise," in the level of biological molecules can greatly impact cellular functions. While biological noise can sometimes be detrimental, recent studies have provided an increasing number of examples in which biological noise can be functionally beneficial. Rather than provide an exhaus...

journal_title：Journal of molecular biology

authors： Harton MD,Batchelor E

更新日期：2017-04-21 00:00:00

abstract：:The small (40 S) subunit of the eukaryotic ribosome may have to scan more than 2000 nucleotides (>600 nm) from its 5'cap recruiting point on an mRNA molecule before initiating on a translation start codon. As with many other processes in living cells, including transcription, editing, mRNA splicing, pre-rRNA processin...

journal_title：Journal of molecular biology

authors： Marsden S,Nardelli M,Linder P,McCarthy JE

更新日期：2006-08-11 00:00:00

abstract：:The requirement of peptidoglycan synthesis for growth complicates the analysis of interactions between proteins involved in this pathway. In particular, the latter steps that involve membrane-linked substrates have proven largely recalcitrant to in vivo analysis. Here, we have taken advantage of the peptidoglycan synt...

journal_title：Journal of molecular biology

authors： Fay A,Meyer P,Dworkin J

更新日期：2010-06-18 00:00:00

abstract：:The Escherichia coli cyclic AMP receptor protein, CRP, induces transcription at Class II CRP-dependent promoters by making three different activatory contacts with different surfaces of holo RNA polymerase. One contact surface of CRP, known as Activating Region 3 (AR3), is functional in the downstream subunit of the C...

journal_title：Journal of molecular biology

authors： Rhodius VA,Busby SJ

更新日期：2000-06-02 00:00:00

abstract：:The FLP recombinase interacts with its target sequence with the formation of three distinct DNA-protein complexes. The first complex leaves neither a DNase footprint nor is the DNA protected from methylation by dimethyl sulfate. We have found, however, that the FLP protein is bound predominantly to only one of the thr...

journal_title：Journal of molecular biology

authors： Beatty LG,Sadowski PD

更新日期：1988-11-20 00:00:00

abstract：:KorA and KorB proteins of IncP1 plasmid RK2 are encoded in the central control region (ccr) of the plasmid and act as global regulators of plasmid genes for replication, transfer and stable inheritance. KorA represses seven promoters on RK2, by binding to a defined operator site, OA, which always occurs in promoter re...

journal_title：Journal of molecular biology

authors： Kostelidou K,Jones AC,Thomas CM

更新日期：1999-06-04 00:00:00

abstract：:Bacteriophage with double-stranded, linear DNA genomes package DNA into pre-assembled icosahedral procapsids through a unique vertex. The packaging vertex contains an oligomeric ring of a portal protein that serves as a recognition site for the packaging enzymes, a conduit for DNA translocation, and the site of tail a...

journal_title：Journal of molecular biology

authors： Moore SD,Prevelige PE Jr

更新日期：2002-02-01 00:00:00

abstract：:Many gram-negative bacteria that are important human pathogens possess type III secretion systems as part of their required virulence factor repertoire. During the establishment of infection, these pathogens coordinately assemble greater than 20 different proteins into a macromolecular structure that spans the bacteri...

journal_title：Journal of molecular biology

authors： Zhang L,Wang Y,Picking WL,Picking WD,De Guzman RN

更新日期：2006-06-02 00:00:00

abstract：:beta-Crystallins are oligomeric eye lens proteins that are related to monomeric gamma-crystallins. The main sequence difference between the two families is the presence of sequence extensions in the beta-crystallins. A major question concerns the role that these extensions play in mediating interactions at the high pr...

journal_title：Journal of molecular biology

authors： Nalini V,Bax B,Driessen H,Moss DS,Lindley PF,Slingsby C

更新日期：1994-03-04 00:00:00

abstract：:The beta-globin gene cluster of human, gorilla and chimpanzee contain the same number and organization of beta-type globin genes: 5'-epsilon (embryonic)-G gamma and A gamma (fetal)-psi beta (inactive)-delta and beta (adult)-3'. We have isolated the psi beta-globin gene regions from the three species and determined the...

journal_title：Journal of molecular biology

authors： Chang LY,Slightom JL

更新日期：1984-12-25 00:00:00

abstract：:Protein phosphorylation at serine, threonine or tyrosine residues affects a multitude of cellular signaling processes. How is specificity in substrate recognition and phosphorylation by protein kinases achieved? Here, we present an artificial neural network method that predicts phosphorylation sites in independent seq...

journal_title：Journal of molecular biology

authors： Blom N,Gammeltoft S,Brunak S

更新日期：1999-12-17 00:00:00

abstract：:Cytosolic 5'-nucleotidase III (cN-III) is responsible for selective degradation of pyrimidine 5'-monoribonucleotides during maturation of reticulocytes to erythrocytes. The lack of this enzymatic activity due to genetic aberrations or lead poisoning results in a mild to moderate nonspherocytic hemolytic anemia. In aff...

journal_title：Journal of molecular biology

authors： Grobosky CL,Lopez JB,Rennie S,Skopelitis DJ,Wiest AT,Bingman CA,Bitto E

更新日期：2012-11-02 00:00:00

abstract：:In order to systematically analyze functionally relevant dynamical correlations within macromolecular complexes, we have developed computational methods based on the normal mode analysis of an elastic network model. First, we define two types of dynamical correlations (fluctuation-based and density-based), which are c...

journal_title：Journal of molecular biology

authors： Zheng W,Brooks B

更新日期：2005-02-25 00:00:00

abstract：:We have characterized the epitopes of a panel of 12 monoclonal antibodies (Mabs) directed to normal human cellular prion protein (PrP(C)) using ELISA and Western blotting of recombinant PrP or synthetic peptide fragments of PrP. The first group of antibodies, which is represented by Mabs 5B2 and 8B4, reacts with PrP(2...

journal_title：Journal of molecular biology

authors： Li R,Liu T,Wong BS,Pan T,Morillas M,Swietnicki W,O'Rourke K,Gambetti P,Surewicz WK,Sy MS

更新日期：2000-08-18 00:00:00

abstract：:Tissue deposition of normally soluble proteins as insoluble amyloid fibrils is associated with serious diseases including the systemic amyloidoses, maturity onset diabetes, Alzheimer's disease and transmissible spongiform encephalopathy. Although the precursor proteins in different diseases do not share sequence homol...

journal_title：Journal of molecular biology

authors： Sunde M,Serpell LC,Bartlam M,Fraser PE,Pepys MB,Blake CC

更新日期：1997-10-31 00:00:00

abstract：:We report here the isolation and primary structure of two genes encoding rat U3 small nuclear RNA. One of the genes encodes U3B RNA; the other encodes an RNA which is almost identical to U3A RNA. Both genes are expressed after microinjection into the nuclei of Xenopus laevis oocytes and can direct the accumulation of ...

journal_title：Journal of molecular biology

authors： Stroke IL,Weiner AM

更新日期：1985-07-20 00:00:00

abstract：:Signal transduction pathways mediated by cyclic-bis(3'→5')-dimeric GMP (c-di-GMP) control many important and complex behaviors in bacteria. C-di-GMP is synthesized through the action of GGDEF domains that possess diguanylate cyclase activity and is degraded by EAL or HD-GYP domains with phosphodiesterase activity. The...

journal_title：Journal of molecular biology

authors： Guzzo CR,Dunger G,Salinas RK,Farah CS

更新日期：2013-06-26 00:00:00

abstract：:A recently developed approach to calculate the pH dependence of protein stability from three-dimensional structure information is applied to the analysis of acid denaturation of sperm whale apomyoglobin. The finite difference Poisson-Boltzmann method is used to calculate pKa values and these are used to obtain titrati...

journal_title：Journal of molecular biology

authors： Yang AS,Honig B

更新日期：1994-04-15 00:00:00

abstract：:Trophozoites of the parasitic protozoa, Entamoeba histolytica, synthesize a cell surface lipoglycoconjugate, termed lipophosphoglycan, which is thought to be an important virulence factor and potential vaccine candidate against invasive amebiasis. Here, we show that the E. histolytica lipophosphoglycans are in fact gl...

journal_title：Journal of molecular biology

authors： Moody-Haupt S,Patterson JH,Mirelman D,McConville MJ

更新日期：2000-03-24 00:00:00

abstract：:The conserved and essential eukaryotic protein Spt6 functions in transcription elongation, chromatin maintenance, and RNA processing. Spt6 has three characterized functions. It is a histone chaperone capable of reassembling nucleosomes, a central component of transcription elongation complexes, and is required for rec...

journal_title：Journal of molecular biology

authors： Close D,Johnson SJ,Sdano MA,McDonald SM,Robinson H,Formosa T,Hill CP

更新日期：2011-05-13 00:00:00

abstract：:We previously described the use of a phage-displayed library of random hexapeptides to define and localise the epitope on the human tumor suppressor protein p53 recognised by the monoclonal antibody PAb240. Here we have extended these results to a further eight anti-p53 monoclonal antibodies and to two further librari...

journal_title：Journal of molecular biology

authors： Stephen CW,Helminen P,Lane DP

更新日期：1995-04-21 00:00:00

abstract：:Mycobacterium tuberculosis, the causative agent of tuberculosis, is known to secrete a number of highly immunogenic proteins that are thought to confer pathogenicity, in part, by mediating binding to host tissues. Among these secreted proteins are the trimeric antigen 85 (Ag85) complex and the related MPT51 protein, a...

journal_title：Journal of molecular biology

authors： Wilson RA,Maughan WN,Kremer L,Besra GS,Fütterer K

更新日期：2004-01-09 00:00:00

abstract：:The three-dimensional structure of the membrane glycoprotein neuraminidase of an escape mutant of the influenza virus strain A/Tokyo/3/67 has been determined to 3 A (1 A = 0.1 nm) resolution by X-ray diffraction. The mutant virus, selected by growing the virus in the presence of a monoclonal antibody to the neuraminid...

journal_title：Journal of molecular biology

authors： Varghese JN,Webster RG,Laver WG,Colman PM

更新日期：1988-03-05 00:00:00