当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei.

Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei.

Abstract：

:Many gram-negative bacteria that are important human pathogens possess type III secretion systems as part of their required virulence factor repertoire. During the establishment of infection, these pathogens coordinately assemble greater than 20 different proteins into a macromolecular structure that spans the bacterial inner and outer membranes and, in many respects, resembles and functions like a syringe. This type III secretion apparatus (TTSA) is used to inject proteins into a host cell's membrane and cytoplasm to subvert normal cellular processes. The external portion of the TTSA is a needle that is composed of a single type of protein that is polymerized in a helical fashion to form an elongated tube with a central channel of 2-3 nm in diameter. TTSA needle proteins from a variety of bacterial pathogens share sequence conservation; however, no atomic structure for any TTSA needle protein is yet available. Here, we report the structure of a TTSA needle protein called BsaL from Burkholderia pseudomallei determined by nuclear magnetic resonance (NMR) spectroscopy. The central part of the protein assumes a helix-turn-helix core domain with two well-defined alpha-helices that are joined by an ordered, four-residue linker. This forms a two-helix bundle that is stabilized by interhelix hydrophobic contacts. Residues that flank this presumably exposed core region are not completely disordered, but adopt a partial helical conformation. The atomic structure of BsaL and its sequence homology with other TTSA needle proteins suggest potentially unique structural dynamics that could be linked with a universal mechanism for control of type III secretion in diverse gram-negative bacterial pathogens.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Zhang L,Wang Y,Picking WL,Picking WD,De Guzman RN

doi

10.1016/j.jmb.2006.03.028

subject

Has Abstract

pub_date

2006-06-02 00:00:00

pages

322-30

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(06)00349-4

journal_volume

359

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Crystallization and preliminary crystallographic investigations of the soluble glucose dehydrogenase from Acinetobacter calcoaceticus.

    abstract：:Single crystals of the soluble glucose dehydrogenase (GDH) from Acinetobacter calcoaceticus have been grown by the vapour diffusion method. These crystals diffract to beyond 2.1 A and are suitable for X-ray crystallography. The space group was determined to be P2(1) with unit cell parameters a = 55.5 A, b = 104.5 A, c...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1553

    authors： Schlunegger MP,Grütter MG,Streiff MB,Olsthoorn AJ,Duine JA

    更新日期：1993-10-20 00:00:00

  • Yeast Prions Compared to Functional Prions and Amyloids.

    abstract：:Saccharomyces cerevisiae is an occasional host to an array of prions, most based on self-propagating, self-templating amyloid filaments of a normally soluble protein. [URE3] is a prion of Ure2p, a regulator of nitrogen catabolism, while [PSI+] is a prion of Sup35p, a subunit of the translation termination factor Sup35...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2018.04.022

    authors： Wickner RB,Edskes HK,Son M,Bezsonov EE,DeWilde M,Ducatez M

    更新日期：2018-10-12 00:00:00

  • Molecular phylogeny and evolutionary timescale for the family of mammalian herpesviruses.

    abstract：:A detailed phylogenetic analysis for mammalian members of the family Herpesviridae, based on molecular sequences is reported. Sets of encoded amino acid sequences were collected for eight well conserved genes that are common to mammalian herpesviruses. Phylogenetic trees were inferred from alignments of these sequence...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0152

    authors： McGeoch DJ,Cook S,Dolan A,Jamieson FE,Telford EA

    更新日期：1995-03-31 00:00:00

  • Core RNA polymerase and promoter DNA interactions of purified domains of sigma N: bipartite functions.

    abstract：:The sigma N class of sigma factors confer upon RNA polymerase the requirement for enhancer-binding activator proteins. The sigma-N (sigma N) protein of Klebsiella pneumoniae was analysed by the assay of purified peptides comprising domains or regions of sigma N defined by proteolysis or by homology alignment, respecti...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0260

    authors： Cannon W,Missailidis S,Smith C,Cottier A,Austin S,Moore M,Buck M

    更新日期：1995-05-12 00:00:00

  • Site-directed mutations in motif VI of Escherichia coli DNA helicase II result in multiple biochemical defects: evidence for the involvement of motif VI in the coupling of ATPase and DNA binding activities via conformational changes.

    abstract：:Two site-directed mutants of Escherichia coli DNA helicase II (UvrD) were constructed to examine the functional significance of motif VI in a superfamily I helicase. Threonine 604 and arginine 605, representing two of the most highly conserved residues in motif VI, were replaced with alanine, generating the mutant all...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1614

    authors： Hall MC,Ozsoy AZ,Matson SW

    更新日期：1998-03-27 00:00:00

  • Nucleotide-dependent lateral and longitudinal interactions in microtubules.

    abstract：:Microtubule (MT) stability is related to the hydrolysis of the guanosine triphosphate nucleotide (NT) bound to β-tubulin. However, the molecular mechanism by which the NT state influences the stability of the contacts in the MT lattice remains elusive. Here, we present large-scale atomistic simulations of different tu...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2013.03.029

    authors： Grafmüller A,Noya EG,Voth GA

    更新日期：2013-06-26 00:00:00

  • Structures of hepatitis B virus cores presenting a model epitope and their complexes with antibodies.

    abstract：:The core shell of hepatitis B virus is a potent immune stimulator, giving a strong neutralizing immune response to foreign epitopes inserted at the immunodominant region, located at the tips of spikes on the exterior of the shell. Here, we analyze structures of core shells with a model epitope inserted at two alternat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.06.032

    authors： Roseman AM,Borschukova O,Berriman JA,Wynne SA,Pumpens P,Crowther RA

    更新日期：2012-10-12 00:00:00

  • On itinerant water molecules and detectability of protein-protein interfaces through comparative analysis of homologues.

    abstract：:We discuss the question of which residues are sufficiently important for protein-protein interaction to be under notable evolutionary pressure. Its interest stems from the applicability of this knowledge in the reverse direction, to detect a protein-protein interface on a single protomer, starting from the rate of mut...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.03.057

    authors： Mihalek I,Res I,Lichtarge O

    更新日期：2007-06-01 00:00:00

  • Crystallization and preliminary crystallographic investigation of methanol dehydrogenase from Methylobacterium extorquens AM1.

    abstract：:Single crystals of methanol dehydrogenase (MDH) from Methylobacterium extorquens AM1 have been grown by the vapour diffusion method. These crystals diffract to beyond 2 A resolution and are suitable for X-ray crystallography. They belong to the orthorhombic space group P2(1)2(1)2(1) and have the following unit cell pa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90509-i

    authors： Ghosh M,Harlos K,Blake CC,Richardson I,Anthony C

    更新日期：1992-11-05 00:00:00

  • Immunoglobulin G1 Fc domain motions: implications for Fc engineering.

    abstract：:The fragment crystallizable (Fc) region links the key pathogen identification and destruction properties of immunoglobulin G (IgG). Pathogen opsonization positions Fcs to activate pro-inflammatory Fcγ receptors (FcγRs) on immune cells. The cellular response and committal to a damaging, though protective, immune respon...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2014.01.011

    authors： Frank M,Walker RC,Lanzilotta WN,Prestegard JH,Barb AW

    更新日期：2014-04-17 00:00:00

  • Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism.

    abstract：:Bacillus sp. GL1 xanthan lyase, a member of polysaccharide lyase family 8 (PL-8), acts exolytically on the side-chains of pentasaccharide-repeating polysaccharide xanthan and cleaves the glycosidic bond between glucuronic acid (GlcUA) and pyruvylated mannose (PyrMan) through a beta-elimination reaction. To clarify the...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.05.055

    authors： Maruyama Y,Hashimoto W,Mikami B,Murata K

    更新日期：2005-07-29 00:00:00

  • Kinetics and thermodynamics of folding of a de novo designed four-helix bundle protein.

    abstract：:A simple continuum model of a de novo designed model of a four-helix bundle is presented. The thermodynamics and kinetics of the model are studied using Langevin simulations. We use a three-letter minimal off-lattice representation of a de novo designed four-helix bundle protein. The native state of the model, which c...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0578

    authors： Guo Z,Thirumalai D

    更新日期：1996-10-25 00:00:00

  • Conformational dynamics of the molecular chaperone Hsp90 in complexes with a co-chaperone and anticancer drugs.

    abstract：:The molecular chaperone Hsp90 is essential for the correct folding, maturation and activation of a diverse array of client proteins, including several key constituents of oncogenic processes. Hsp90 has become a focus of cancer research, since it represents a target for direct prophylaxis against multistep malignancy. ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.04.059

    authors： Phillips JJ,Yao ZP,Zhang W,McLaughlin S,Laue ED,Robinson CV,Jackson SE

    更新日期：2007-10-05 00:00:00

  • Non-catalytic Binding Sites Induce Weaker Long-Range Evolutionary Rate Gradients than Catalytic Sites in Enzymes.

    abstract：:Enzymes exhibit a strong long-range evolutionary constraint that extends from their catalytic site and affects even distant sites, where site-specific evolutionary rate increases monotonically with distance. While protein-protein sites in enzymes were previously shown to induce only a weak conservation gradient, a com...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.07.019

    authors： Sharir-Ivry A,Xia Y

    更新日期：2019-09-06 00:00:00

  • Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation.

    abstract：:Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative prion disease affecting cattle that is transmissible to humans, manifesting as a variant of Creutzfeldt-Jakob disease (vCJD) likely following the consumption of meat contaminated with BSE prions. High-affinity antibodies are a prerequisite for the dev...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.07.027

    authors： Luginbühl B,Kanyo Z,Jones RM,Fletterick RJ,Prusiner SB,Cohen FE,Williamson RA,Burton DR,Plückthun A

    更新日期：2006-10-13 00:00:00

  • Supercoiling-dependent site-specific binding of HU to naked Mu DNA.

    abstract：:Using HU chemical nucleases to probe HU-DNA interactions, we report here for the first time site-specific binding of HU to naked DNA. An unique feature of this interaction is the absolute requirement for negative DNA supercoiling for detectable levels of site-specific DNA binding. The HU binding site is the Mu spacer ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2805

    authors： Kobryn K,Lavoie BD,Chaconas G

    更新日期：1999-06-18 00:00:00

  • Diffusing and colliding: the atomic level folding/unfolding pathway of a small helical protein.

    abstract：:Proteins with ultra-fast folding/unfolding kinetics are excellent candidates for study by molecular dynamics. Here, we describe such simulations of a three helix bundle protein, the engrailed homeodomain (En-HD), which folds via the diffusion-collision model. The unfolding pathway of En-HD was characterized by seven s...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.06.074

    authors： DeMarco ML,Alonso DO,Daggett V

    更新日期：2004-08-20 00:00:00

  • Order-disorder-order transitions mediate the activation of cholera toxin.

    abstract：:Cholera toxin (CT) holotoxin must be activated to intoxicate host cells. This process requires the intracellular dissociation of the enzymatic CTA1 domain from the holotoxin components CTA2 and B5, followed by subsequent interaction with the host factor ADP ribosylation factor 6 (ARF6)-GTP. We report the first NMR-bas...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.12.075

    authors： Ampapathi RS,Creath AL,Lou DI,Craft JW Jr,Blanke SR,Legge GB

    更新日期：2008-03-28 00:00:00

  • pH-dependent interactions and the stability and folding kinetics of the N-terminal domain of L9. Electrostatic interactions are only weakly formed in the transition state for folding.

    abstract：:The role of electrostatic interactions in the stability and the folding of the N-terminal domain of the ribosomal protein L9 (NTL9) was investigated by determining the effects of varying the pH conditions. Urea denaturations and thermal unfolding experiments were used to measure the free energy of folding, DeltaG degr...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3752

    authors： Luisi DL,Raleigh DP

    更新日期：2000-06-16 00:00:00

  • Analysis of the distribution of binding sites for a tissue-specific transcription factor in the vertebrate genome.

    abstract：:Hepatocyte nuclear factor 1 (HNF1) is a dimeric homeoprotein expressed in hepatocytes and in a few other epithelial cells where it helps regulate the expression of a specific subset of genes. In an attempt to identify novel target genes for HNF1 and to assess the distribution of its target sites within the vertebrate ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0760

    authors： Tronche F,Ringeisen F,Blumenfeld M,Yaniv M,Pontoglio M

    更新日期：1997-02-21 00:00:00

  • Site-specific structural variations accompanying tubular assembly of the HIV-1 capsid protein.

    abstract：:The 231-residue capsid (CA) protein of human immunodeficiency virus type 1 (HIV-1) spontaneously self-assembles into tubes with a hexagonal lattice that is believed to mimic the surface lattice of conical capsid cores within intact virions. We report the results of solid-state nuclear magnetic resonance (NMR) measurem...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2013.12.021

    authors： Bayro MJ,Chen B,Yau WM,Tycko R

    更新日期：2014-03-06 00:00:00

  • The Role of RNA Interference in Stem Cell Biology: Beyond the Mutant Phenotypes.

    abstract：:Complex gene regulation systems ensure the maintenance of cellular identity during early development in mammals. Eukaryotic small RNAs have emerged as critical players in RNA interference (RNAi) by mediating gene silencing during embryonic stem cell self-renewal. Most of the proteins involved in the biogenesis of smal...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2017.01.014

    authors： Bodak M,Cirera-Salinas D,Luitz J,Ciaudo C

    更新日期：2017-05-19 00:00:00

  • Discovery of ANTAR-RNAs and their Mechanism of Action in Mycobacteria.

    abstract：:Non-coding RNAs play pivotal roles in bacterial signaling. However, RNAs from certain phyla (specially high-GC actinobacteria) still remain elusive. Here, by re-engineering the existing genome-wide search approach, we discover a family of structurally conserved RNAs that are present ubiquitously across actinobacteria,...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.05.003

    authors： Mehta D,Koottathazhath A,Ramesh A

    更新日期：2020-06-26 00:00:00

  • Cystoviral polymerase complex protein P7 uses its acidic C-terminal tail to regulate the RNA-directed RNA polymerase P2.

    abstract：:In bacteriophages of the cystovirus family, the polymerase complex (PX) encodes a 75-kDa RNA-directed RNA polymerase (P2) that transcribes the double-stranded RNA genome. Also a constituent of the PX is the essential protein P7 that, in addition to accelerating PX assembly and facilitating genome packaging, plays a re...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2014.04.028

    authors： Alphonse S,Arnold JJ,Bhattacharya S,Wang H,Kloss B,Cameron CE,Ghose R

    更新日期：2014-07-15 00:00:00

  • Human liver cathepsin D. Purification, crystallization and preliminary X-ray diffraction analysis of a lysosomal enzyme.

    abstract：:The two-chain form of active cathepsin D, a glycosylated, lysosomal aspartic proteinase, has been isolated from human liver. Isoelectric focusing revealed two major species of enzyme that differed by approximately 0.2 pI unit. Crystals suitable for X-ray diffraction analysis were prepared from acidic solutions using p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90696-h

    authors： Gulnik S,Baldwin ET,Tarasova N,Erickson J

    更新日期：1992-09-05 00:00:00

  • Sixteen discrete RNA components in the cytoplasmic ribosome of Euglena gracilis.

    abstract：:We have isolated cytoplasmic ribosomes from Euglena gracilis and characterized the RNA components of these particles. We show here that instead of the four rRNAs (17-19 S, 25-28 S, 5.8 S and 5 S) found in typical eukaryotic ribosomes, Euglena cytoplasmic ribosomes contain 16 RNA components. Three of these Euglena rRNA...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(05)80096-8

    authors： Schnare MN,Gray MW

    更新日期：1990-09-05 00:00:00

  • Crystal structure of LAAO from Calloselasma rhodostoma with an L-phenylalanine substrate: insights into structure and mechanism.

    abstract：:L-Amino acid oxidase is a dimeric glycosylated flavoenzyme, a major constituent of the venom-from the snake Calloselasma rhodostoma. The enzyme exhibits apoptosis inducing effects as well as antibacterial and anti-HIV activities. The structure of l-amino acid oxidase with its substrate (L-phenylalanine) has been refin...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.09.032

    authors： Moustafa IM,Foster S,Lyubimov AY,Vrielink A

    更新日期：2006-12-15 00:00:00

  • Real time kinetic studies of the interaction between folded antisense and target RNAs using surface plasmon resonance.

    abstract：:Antisense RNAs interact with their complementary target RNAs as folded structures. The formation of early binding intermediates is the most important step in determining the overall rates of stable complex formation in vitro and the efficiency of control in vivo. In the case of CopA and CopT (antisense/target RNA pair...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4802

    authors： Nordgren S,Slagter-Jäger JG,Wagner GH

    更新日期：2001-07-27 00:00:00

  • Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome.

    abstract：:Determining the relationship between protein folding pathways on and off the ribosome remains an important area of investigation in biology. Studies on isolated domains have shown that alteration of the separation of residues in a polypeptide chain, while maintaining their spatial contacts, may affect protein stabilit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2018.10.011

    authors： Marsden AP,Hollins JJ,O'Neill C,Ryzhov P,Higson S,Mendonça CATF,Kwan TO,Kwa LG,Steward A,Clarke J

    更新日期：2018-12-07 00:00:00

  • Crystal structure of a human embryonic haemoglobin: the carbonmonoxy form of gower II (alpha2 epsilon2) haemoglobin at 2.9 A resolution.

    abstract：:The production of recombinant embryonic haemoglobins via a yeast expression system has enabled structural and functional studies to be conducted on these proteins. As part of a programme aimed at understanding the properties of the embryonic haemoglobins we have crystallized the human alpha2 epsilon2 (Gower II) embryo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1868

    authors： Sutherland-Smith AJ,Baker HM,Hofmann OM,Brittain T,Baker EN

    更新日期：1998-07-17 00:00:00