Crystallization and preliminary crystallographic investigation of methanol dehydrogenase from Methylobacterium extorquens AM1.

abstract：:Shortened Limulus thick filaments, isolated from stimulated muscle, are structurally similar to long filaments, isolated from unstimulated muscle, except for length. Both have 3-fold screw symmetry with a helical repeat at approximately 43 nm, axial spacing of 14.5 nm between successive crowns of crossbridges and 4-fo...

journal_title：Journal of molecular biology

authors： Levine RJ,Kensler RW

更新日期：1985-03-20 00:00:00

abstract：:Crystal structures of an Fab (NC6.8) from a murine IgG2b(kappa) antibody and its complex with a sweet-tasting, N-,N'-,N"-trisubstituted guanidine compound (NC174) have been determined by X-ray analysis. Both crystal forms are produced by a microseeding technique in polyethylene glycol (PEG) 8000 but the habits and spa...

journal_title：Journal of molecular biology

authors： Guddat LW,Shan L,Anchin JM,Linthicum DS,Edmundson AB

更新日期：1994-02-11 00:00:00

abstract：:No drug has been targeted specifically for HIV-2 (human immunodeficiency virus type 2) infection despite its increasing prevalence worldwide. The antiviral HIV-1 (human immunodeficiency virus type 1) protease (PR) inhibitor darunavir and the chemically related GRL98065 and GRL06579A were designed with the same chemica...

journal_title：Journal of molecular biology

authors： Kovalevsky AY,Louis JM,Aniana A,Ghosh AK,Weber IT

更新日期：2008-12-05 00:00:00

abstract：:Hsp40-like co-chaperones are ubiquitous enzymes that stimulate the protein refolding activity of Hsp70 family chaperones. They are widespread in prokaryotic and eukaryotic systems. In bacteria, the best characterized co-chaperone is the Escherichia coli DnaJ protein. Many γ-proteobacteria encode a functional homologue...

journal_title：Journal of molecular biology

authors： Chintakayala K,Grainger DC

更新日期：2011-08-12 00:00:00

abstract：:Catalytic and other functionally important residues in proteins can often be mutated to yield more stable proteins. Many of these residues are charged residues that are located in electrostatically unfavorable environments. Here it is demonstrated that because continuum electrostatics methods can identify these destab...

journal_title：Journal of molecular biology

authors： Elcock AH

更新日期：2001-09-28 00:00:00

abstract：:The X-ray diffraction pattern of tendon collagen can contain a number of sharp Bragg peaks indicating three-dimensional crystallinity of the sample. Optimal diffraction images have been obtained with a high flux synchrotron X-ray source and a carefully maintained sample environment and staining techniques. The Bragg p...

journal_title：Journal of molecular biology

authors： Wess TJ,Hammersley A,Wess L,Miller A

更新日期：1995-04-28 00:00:00

abstract：:Single crystals of ribonuclease I from Escherichia coli have been obtained by the vapor diffusion method. The crystals belong to the tetragonal space group P4(1)2(1)2 or its enantiomer P4(3)2(1)2 and have cell parameters a = b = 119.01 A and c = 34.40 A. There is one 27,000 dalton monomer in the asymmetric unit. The c...

journal_title：Journal of molecular biology

authors： Lim LW,Mathur S,Cannistraro VJ,Kennell D

更新日期：1993-11-20 00:00:00

abstract：:Transcription of the ribosomal RNA operons (rrn) in Escherichia coli is subject to an antitermination mechanism whereby RNA polymerase is modified to a termination-resistant form during transit through the rrn leader region. This antitermination mechanism is unable to overcome the T1-T2 terminator cluster located at t...

journal_title：Journal of molecular biology

authors： Ghosh B,Grzadzielska E,Bhattacharya P,Peralta E,DeVito J,Das A

更新日期：1991-11-05 00:00:00

abstract：:Two major methods are currently being used to characterize transient intermediates during protein folding at the level of individual residues. Nuclear magnetic resonance (n.m.r.) measurements on the protection of peptide NH hydrogens against exchange with solvent during refolding can provide information about secondar...

journal_title：Journal of molecular biology

authors： Matouschek A,Serrano L,Meiering EM,Bycroft M,Fersht AR

更新日期：1992-04-05 00:00:00

abstract：:Recent experimental results suggest that the native fold, or topology, plays a primary role in determining the structure of the transition state ensemble, at least for small, fast-folding proteins. To investigate the extent of the topological control of the folding process, we studied the folding of simplified models ...

journal_title：Journal of molecular biology

authors： Clementi C,Nymeyer H,Onuchic JN

更新日期：2000-05-19 00:00:00

abstract：:Dystrophin Dp71 is the main product of the Duchenne muscular dystrophy gene in the brain; however, its function is unknown. To study the role of Dp71 in neuronal cells, we previously generated by antisense treatment PC12 neuronal cell clones with decreased Dp71 expression (antisense-Dp71 cells). PC12 cells express two...

journal_title：Journal of molecular biology

authors： Cerna J,Cerecedo D,Ortega A,García-Sierra F,Centeno F,Garrido E,Mornet D,Cisneros B

更新日期：2006-10-06 00:00:00

abstract：:The FASTA package of sequence comparison programs has been modified to provide accurate statistical estimates for local sequence similarity scores with gaps. These estimates are derived using the extreme value distribution from the mean and variance of the local similarity scores of unrelated sequences after the score...

journal_title：Journal of molecular biology

authors： Pearson WR

更新日期：1998-02-13 00:00:00

abstract：:SCML2 (sex comb on midleg-like 2) is a constituent of the Polycomb repressive complex 1, a large multiprotein assembly required for the repression of developmental control genes. It contains two MBT (malignant brain tumor) repeats; the MBT is a protein module structurally similar to domains that bind to methylated his...

journal_title：Journal of molecular biology

authors： Santiveri CM,Lechtenberg BC,Allen MD,Sathyamurthy A,Jaulent AM,Freund SM,Bycroft M

更新日期：2008-10-24 00:00:00

abstract：:When theoretical methods are used to predict the properties of a given system, such as the effects of the substitution of a specific amino acid on the activity or stability of a protein as a whole, the accuracy of the prediction is directly dependent on the validity of the underlying model. A common error, however, is...

journal_title：Journal of molecular biology

authors： van Gunsteren WF,Mark AE

更新日期：1992-09-20 00:00:00

abstract：:The structure of subfragment 1 (S1) bound to F-actin has been compared to the structure of free S1 using neutron scattering. The F-actin was rendered "invisible" to neutrons by selective deuteration and solvent contrast matching. Highly deuterated actin was purified from the slime mold Dictyostelium discoideum, which ...

journal_title：Journal of molecular biology

authors： Curmi PM,Stone DB,Schneider DK,Spudich JA,Mendelson RA

更新日期：1988-10-05 00:00:00

abstract：:We have evaluated the thermodynamic parameters associated with cooperative cofilin binding to actin filaments, accounting for contributions of ion-linked equilibria, and determined the kinetic basis of cooperative cofilin binding. Ions weaken non-contiguous (isolated, non-cooperative) cofilin binding to an actin filam...

journal_title：Journal of molecular biology

authors： Cao W,Goodarzi JP,De La Cruz EM

更新日期：2006-08-11 00:00:00

abstract：:The structure of the recombinant (-) gamma-lactamase from an Aureobacterium species has been solved at 1.73A resolution in the cubic space group F23 with unit cell parameters a=b=c=240.6A. The trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. The structure has be...

journal_title：Journal of molecular biology

authors： Line K,Isupov MN,Littlechild JA

更新日期：2004-04-30 00:00:00

abstract：:The ligation of a decadeoxynucleotide containing the EcoRI recognition site forms a series of multimers which appear to be curved based on observed anomalous gel migration in polyacrylamide gels. The degree of DNA curvature present in the recognition sequence, based upon the observed migration anomaly, can be altered ...

journal_title：Journal of molecular biology

authors： Diekmann S,McLaughlin LW

更新日期：1988-08-20 00:00:00

abstract：:The dimer formed by the ATF-2 and c-Jun transcription factors is one of the main components of the human interferon-beta enhanceosome. Although these two transcription factors are able to form two homodimers and one heterodimer, it is mainly the heterodimer that participates in the formation of this enhanceosome, bind...

journal_title：Journal of molecular biology

authors： Carrillo RJ,Dragan AI,Privalov PL

更新日期：2010-02-19 00:00:00

abstract：:Ligand-induced conformational change in transferrins has been studied by site-directed mutagenesis of human serum half molecule (N-lobe), X-ray absorption fine structure (XAFS) spectroscopy and X-ray solution scattering. Use of recent advances in data analysis has been made for extracting model-independent molecular s...

journal_title：Journal of molecular biology

authors： Grossmann JG,Crawley JB,Strange RW,Patel KJ,Murphy LM,Neu M,Evans RW,Hasnain SS

更新日期：1998-06-05 00:00:00

abstract：:Protein folding in the cell requires the assistance of enzymes collectively called chaperones. Among these, the chaperonins are 1-MDa ring-shaped oligomeric complexes that bind unfolded polypeptides and promote their folding within an isolated chamber in an ATP-dependent manner. Group II chaperonins, found in archaea ...

journal_title：Journal of molecular biology

authors： Lopez T,Dalton K,Frydman J

更新日期：2015-09-11 00:00:00

abstract：:AP205 is a single-stranded RNA bacteriophage that has a coat protein sequence not similar to any other known single-stranded RNA phage. Here, we report an atomic-resolution model of the AP205 virus-like particle based on a crystal structure of an unassembled coat protein dimer and a cryo-electron microscopy reconstruc...

journal_title：Journal of molecular biology

authors： Shishovs M,Rumnieks J,Diebolder C,Jaudzems K,Andreas LB,Stanek J,Kazaks A,Kotelovica S,Akopjana I,Pintacuda G,Koning RI,Tars K

更新日期：2016-10-23 00:00:00

abstract：:According to a trans -translation model, tmRNA facilitates the resumption of translation that has been stalled on the ribosome with the 3' end of a terminator-less mRNA, to produce a chimera polypeptide of the nascent peptide and the tmRNA-encoding 11 amino acid-tag. The first alanine residue of the tag-sequence is en...

journal_title：Journal of molecular biology

authors： Nameki N,Tadaki T,Muto A,Himeno H

更新日期：1999-05-28 00:00:00

abstract：:It is generally believed that DNA replication in most eukaryotes proceeds according to a precise program in which there is a defined temporal order by which each chromosomal region is duplicated. However, the regularity of this program at the level of individual chromosomes, in terms of both the relative timing and th...

journal_title：Journal of molecular biology

authors： Czajkowsky DM,Liu J,Hamlin JL,Shao Z

更新日期：2008-01-04 00:00:00

abstract：:The pathogenically important cholesterol-binding pore-forming bacterial "thiol-activated" toxins (TATs) are commonly believed to be monomeric in solution and to undergo a transition on membrane binding mediated by cholesterol to an oligomeric pore. We present evidence, gained through the application of a number of bio...

journal_title：Journal of molecular biology

authors： Gilbert RJ,Rossjohn J,Parker MW,Tweten RK,Morgan PJ,Mitchell TJ,Errington N,Rowe AJ,Andrew PW,Byron O

更新日期：1998-12-11 00:00:00

abstract：:A major problem in sequence alignments based on the standard dynamic programming method is that the optimal path does not necessarily yield the best equivalencing of residues assessed by structural or functional criteria. An algorithm is presented that finds suboptimal alignments of protein sequences by a simple modif...

journal_title：Journal of molecular biology

authors： Saqi MA,Sternberg MJ

更新日期：1991-06-20 00:00:00

abstract：:Here we present the crystal structure of the Methanococcus jannaschii RelE-RelB (RelBE) toxin-antitoxin (TA) protein complex determined by the MIRAS (multiple isomorphous replacement with anomalous signal) method. The genes encoding this TA system are located in the chromosome of this archaeon and involved in stress r...

journal_title：Journal of molecular biology

authors： Francuski D,Saenger W

更新日期：2009-11-06 00:00:00

abstract：:The coat protein of the simple spherical (triangulation no. T = 3) RNA coliphage R17 protects the genomic RNA in the virus particle and acts as a translational repressor of the phage-encoded replicase gene. It has been suggested that these two functions are related and that the translational repression complex serves ...

journal_title：Journal of molecular biology

authors： Beckett D,Uhlenbeck OC

更新日期：1988-12-20 00:00:00

abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

journal_title：Journal of molecular biology

authors： Wei Z,Xue Y,Xu H,Gong W

更新日期：2006-05-26 00:00:00

abstract：:The seeds of jack fruit (Artocarpus integrifolia) contain two tetrameric lectins, jacalin and artocarpin. Jacalin was the first lectin found to exhibit the beta-prism I fold, which is characteristic of the Moraceae plant lectin family. Jacalin contains two polypeptide chains produced by a post-translational proteolysi...

journal_title：Journal of molecular biology

authors： Pratap JV,Jeyaprakash AA,Rani PG,Sekar K,Surolia A,Vijayan M

更新日期：2002-03-22 00:00:00