Abstract:
:SCML2 (sex comb on midleg-like 2) is a constituent of the Polycomb repressive complex 1, a large multiprotein assembly required for the repression of developmental control genes. It contains two MBT (malignant brain tumor) repeats; the MBT is a protein module structurally similar to domains that bind to methylated histones. We have used NMR spectroscopy to examine the binding specificity of these repeats. Our data show that they preferentially bind histone peptides monomethylated at lysine residues with no apparent sequence specificity. The crystal structure of the complex between the protein and monomethyllysine reveals that the modified amino acid binds to an aromatic rich pocket at one end of the beta-barrel of the second repeat.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Santiveri CM,Lechtenberg BC,Allen MD,Sathyamurthy A,Jaulent AM,Freund SM,Bycroft Mdoi
10.1016/j.jmb.2008.07.081subject
Has Abstractpub_date
2008-10-24 00:00:00pages
1107-12issue
5eissn
0022-2836issn
1089-8638pii
S0022-2836(08)00950-9journal_volume
382pub_type
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