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Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome.

Abstract：

:Determining the relationship between protein folding pathways on and off the ribosome remains an important area of investigation in biology. Studies on isolated domains have shown that alteration of the separation of residues in a polypeptide chain, while maintaining their spatial contacts, may affect protein stability and folding pathway. Due to the vectorial emergence of the polypeptide chain from the ribosome, chain connectivity may have an important influence upon cotranslational folding. Using MATH, an all β-sandwich domain, we investigate whether the connectivity of residues and secondary structure elements is a key determinant of when cotranslational folding can occur on the ribosome. From Φ-value analysis, we show that the most structured region of the transition state for folding in MATH includes the N and C terminal strands, which are located adjacent to each other in the structure. However, arrest peptide force-profile assays show that wild-type MATH is able to fold cotranslationally, while some C-terminal residues remain sequestered in the ribosome, even when destabilized by 2-3 kcal mol-1. We show that, while this pattern of Φ-values is retained in two circular permutants in our studies of the isolated domains, one of these permutants can fold only when fully emerged from the ribosome. We propose that in the case of MATH, onset of cotranslational folding is determined by the ability to form a sufficiently stable folding nucleus involving both β-sheets, rather than by the location of the terminal strands in the ribosome tunnel.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Marsden AP,Hollins JJ,O'Neill C,Ryzhov P,Higson S,Mendonça CATF,Kwan TO,Kwa LG,Steward A,Clarke J

doi

10.1016/j.jmb.2018.10.011

subject

Has Abstract

pub_date

2018-12-07 00:00:00

pages

5207-5216

issue

24

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(18)30510-2

journal_volume

430

pub_type

杂志文章

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