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Membrane Targeting and Insertion of the C-Tail Protein SciP.

Abstract：

:C-tailed membrane proteins insert into the bilayer post-translationally because the hydrophobic anchor segment leaves the ribosome at the end of translation. Nevertheless, we find here evidence that the targeting of SciP to the membrane of Escherichia coli occurs co-translationally since signal elements in the N-terminal part of the SciP protein sequence are present. Two short hydrophobic sequences were identified that targeted a green fluorescent protein-SciP fusion protein to the membrane involving the signal recognition particle. After targeting, the membrane insertion of SciP is catalyzed by YidC independent of the SecYEG translocase. However, when the C-terminal tail of SciP was extended to 21 aa residues, we found that SecYEG becomes involved and makes its membrane insertion more efficient.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Pross E,Soussoula L,Seitl I,Lupo D,Kuhn A

doi

10.1016/j.jmb.2016.09.001

subject

Has Abstract

pub_date

2016-10-09 00:00:00

pages

4218-4227

issue

20

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(16)30363-1

journal_volume

428

pub_type

杂志文章

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