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Supercoiling-dependent site-specific binding of HU to naked Mu DNA.

Abstract：

:Using HU chemical nucleases to probe HU-DNA interactions, we report here for the first time site-specific binding of HU to naked DNA. An unique feature of this interaction is the absolute requirement for negative DNA supercoiling for detectable levels of site-specific DNA binding. The HU binding site is the Mu spacer between the L1 and L2 transposase binding sites. Our results suggest recognition of an altered DNA structure which is induced by DNA supercoiling. We propose that recruitment of HU to this naked DNA site induces the DNA bending required for productive synapsis and transpososome assembly. Implications of HU as a supercoiling sensor with a potential in vivo regulatory role are discussed. Finally, using HU nucleases we have also shown that non-specific DNA binding by HU is stimulated by increasing levels of supercoiling.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kobryn K,Lavoie BD,Chaconas G

doi

10.1006/jmbi.1999.2805

keywords:

subject

Has Abstract

pub_date

1999-06-18 00:00:00

pages

777-84

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(99)92805-X

journal_volume

289

pub_type

杂志文章

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