当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > On the interaction between gp41 and membranes: the immunodominant loop stabilizes gp41 helical hairpin conformation.

On the interaction between gp41 and membranes: the immunodominant loop stabilizes gp41 helical hairpin conformation.

Abstract：

:gp41 is the protein responsible for the process of membrane fusion that allows primate lentiviruses (HIV and SIV) to enter into their host cells. gp41 ectodomain contains an N-terminal and a C-terminal heptad repeat region (NHR and CHR) connected by an immunodominant loop. In the absence of membranes, the NHR and CHR segments fold into a protease-resistant core with a trimeric helical hairpin structure. However, when the immunodominant loop is not present (either in a complex formed by HIV-1 gp41-derived NHR and CHR peptides or by mild treatment with protease of recombinant constructs of HIV-1 gp41 ectodomain, which also lack the N-terminal fusion peptide and the C-terminal Trp-rich region) membrane binding induces a conformational change in the gp41 core structure. Here, we further investigated whether covalently linking the NHR and CHR segments by the immunodominant loop affects this conformational change. Specifically, we analyzed a construct corresponding to a fragment of SIVmac239 gp41ectodomain (residues 27-149, named e-gp41) by means of surface plasmon resonance, Trp and rhodamine fluorescence, ATR-FTIR spectroscopy, and differential scanning calorimetry. Our results suggest that the presence of the loop stabilizes the trimeric helical hairpin both when e-gp41 is in aqueous solution and when it is bound to the membrane surface. Bearing in mind possible differences between HIV-1 and SIV gp41, and considering that the gp41 ectodomain constructs analyzed to date lack the N-terminal fusion peptide and the C-terminal Trp-rich region, we discuss our observations in relation to the mechanism of virus-induced membrane fusion.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Peisajovich SG,Blank L,Epand RF,Epand RM,Shai Y

doi

10.1016/s0022-2836(03)00040-8

keywords:

subject

Has Abstract

pub_date

2003-03-07 00:00:00

pages

1489-501

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022283603000408

journal_volume

326

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Internal promoter elements of transfer RNA genes are preferentially exposed in chromatin.

    abstract：:We have examined the chromatin organization of a cluster of transfer RNA genes at the cytogenic locus 90BC on the right arm of the third Drosophila melanogaster chromosome. We find that the internal promoter sequences are preferentially exposed to micrococcal nuclease and DNase I in chromatin. Moreover, these exposed ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90158-x

    authors： DeLotto R,Schedl P

    更新日期：1984-11-15 00:00:00

  • Molecular evolution of antibody affinity for sensitive detection of botulinum neurotoxin type A.

    abstract：:Botulism is caused by botulinum neurotoxin (BoNT), the most poisonous substance known. Potential use of BoNT as a biothreat agent has made development of sensitive assays for toxin detection and potent antitoxin for treatment of intoxication a high priority. To improve detection and treatment of botulism, molecular ev...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.06.003

    authors： Razai A,Garcia-Rodriguez C,Lou J,Geren IN,Forsyth CM,Robles Y,Tsai R,Smith TJ,Smith LA,Siegel RW,Feldhaus M,Marks JD

    更新日期：2005-08-05 00:00:00

  • Recognition of the highly conserved GTPase center of 23 S ribosomal RNA by ribosomal protein L11 and the antibiotic thiostrepton.

    abstract：:The antibiotic thiostrepton, a thiazole-containing peptide, inhibits translation and ribosomal GTPase activity by binding directly to a limited and highly conserved region of the large subunit ribosomal RNA termed the GTPase center. We have previously used a filter binding assay to examine the binding of ribosomal pro...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90932-v

    authors： Ryan PC,Lu M,Draper DE

    更新日期：1991-10-20 00:00:00

  • Defining the structural basis for assembly of a transmembrane cytochrome.

    abstract：:To define the structural basis for cofactor binding to membrane proteins, we introduce a manageable model system, which allows us, for the first time, to study the influence of individual transmembrane helices and of single amino acid residues on the assembly of a transmembrane cytochrome. In vivo as well as in vitro ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.05.016

    authors： Prodöhl A,Volkmer T,Finger C,Schneider D

    更新日期：2005-07-22 00:00:00

  • In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities.

    abstract：:Human pseudouridine (Ψ) synthase Pus1 (hPus1) modifies specific uridine residues in several non-coding RNAs: tRNA, U2 spliceosomal RNA, and steroid receptor activator RNA. We report three structures of the catalytic core domain of hPus1 from two crystal forms, at 1.8Å resolution. The structures are the first of a mamm...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2013.05.014

    authors： Czudnochowski N,Wang AL,Finer-Moore J,Stroud RM

    更新日期：2013-10-23 00:00:00

  • Complex of lac repressor headpiece with a 14 base-pair lac operator fragment studied by two-dimensional nuclear magnetic resonance.

    abstract：:Two-dimensional nuclear Overhauser enhancement spectra are presented of the complex of lac repressor headpiece with a 14 base-pair lac operator fragment. Analysis of nuclear Overhauser enhancements observed between protein and DNA shows that the second helix of the headpiece ("the recognition helix") binds in the majo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90638-3

    authors： Boelens R,Scheek RM,van Boom JH,Kaptein R

    更新日期：1987-01-05 00:00:00

  • The structure of the Sec13/31 COPII cage bound to Sec23.

    abstract：:Structural studies have revealed some of the organizing principles and mechanisms involved in the assembly of the COPII coat including the location of the Sec23/24 adapter layer. Previous studies, however, were unable to unambiguously determine the positions of Sec23 and Sec24 in the coat. Here, we have determined a c...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.04.024

    authors： Bhattacharya N,O Donnell J,Stagg SM

    更新日期：2012-07-20 00:00:00

  • Electrostatic properties of two porin channels from Escherichia coli.

    abstract：:The electrostatic interactions in the channels of OmpF and PhoE porins from Escherichia coli were analysed on the basis of a macroscopic multi-dielectric model of the protein-membrane complex derived from the respective porin X-ray structures. The membrane was represented as layers of distinct dielectric constants cor...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1451

    authors： Karshikoff A,Spassov V,Cowan SW,Ladenstein R,Schirmer T

    更新日期：1994-07-22 00:00:00

  • Transcript elongation factor TFIIS is involved in arabidopsis seed dormancy.

    abstract：:Transcript elongation factor TFIIS promotes efficient transcription by RNA polymerase II, since it assists in bypassing blocks during mRNA synthesis. While yeast cells lacking TFIIS are viable, inactivation of mouse TFIIS causes embryonic lethality. Here, we have identified a protein encoded in the Arabidopsis genome ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.12.066

    authors： Grasser M,Kane CM,Merkle T,Melzer M,Emmersen J,Grasser KD

    更新日期：2009-02-27 00:00:00

  • Identification of an epitope in the C terminus of normal prion protein whose expression is modulated by binding events in the N terminus.

    abstract：:We have characterized the epitopes of a panel of 12 monoclonal antibodies (Mabs) directed to normal human cellular prion protein (PrP(C)) using ELISA and Western blotting of recombinant PrP or synthetic peptide fragments of PrP. The first group of antibodies, which is represented by Mabs 5B2 and 8B4, reacts with PrP(2...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3986

    authors： Li R,Liu T,Wong BS,Pan T,Morillas M,Swietnicki W,O'Rourke K,Gambetti P,Surewicz WK,Sy MS

    更新日期：2000-08-18 00:00:00

  • Structural basis for subunit assembly in UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae.

    abstract：:UDP-glucose is the universal activated form of glucose, employed in all organisms for glucosyl transfer reactions and as precursor for various activated carbohydrates. In animal and fungal metabolism, UDP-glucose is required for utilization of galactose and for the synthesis of glycogen, the major carbohydrate storage...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.08.079

    authors： Roeben A,Plitzko JM,Körner R,Böttcher UM,Siegers K,Hayer-Hartl M,Bracher A

    更新日期：2006-12-08 00:00:00

  • Structure and reactivity of an asymmetric complex between HslV and I-domain deleted HslU, a prokaryotic homolog of the eukaryotic proteasome.

    abstract：:In the prokaryotic homolog of the eukaryotic proteasome, HslUV, the "double donut" HslV protease is allosterically activated by HslU, an AAA protein of the Clp/Hsp100 family consisting of three (amino-terminal, carboxy-terminal, and intermediate) domains. The intermediate domains of HslU, which extend like tentacles f...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00580-1

    authors： Kwon AR,Kessler BM,Overkleeft HS,McKay DB

    更新日期：2003-07-04 00:00:00

  • Automatic definition of recurrent local structure motifs in proteins.

    abstract：:An automatic procedure for defining recurrent folding motifs in proteins of known structure is described. These motifs are formed by short polypeptide fragments of equal size containing between four and seven residues. The method applies a classical clustering algorithm that operates on distances between selected back...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(05)80194-9

    authors： Rooman MJ,Rodriguez J,Wodak SJ

    更新日期：1990-05-20 00:00:00

  • Expression and transmission of a rodent retrovirus-like (VL30) gene family.

    abstract：:A transcriptionally active sub-set of the dispersed mouse VL30 family of proviral genetic elements was shown to be highly transmissible as a murine leukaemia virus pseudotype. Newly acquired VL30 proviruses (present at 1 to 2 copies per cell) were shown to be transcriptionally active. These data substantiate the hypot...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(86)90485-7

    authors： Carter AT,Norton JD,Gibson Y,Avery RJ

    更新日期：1986-03-05 00:00:00

  • Protein Interaction Energy Landscapes are Shaped by Functional and also Non-functional Partners.

    abstract：:In the crowded cell, a strong selective pressure operates on the proteome to limit the competition between functional and non-functional protein-protein interactions. We developed an original theoretical framework in order to interrogate how this competition constrains the behavior of proteins with respect to their pa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.12.047

    authors： Schweke H,Mucchielli MH,Sacquin-Mora S,Bei W,Lopes A

    更新日期：2020-02-14 00:00:00

  • Influence of transfer RNA tertiary structure on aminoacylation efficiency by glutaminyl and cysteinyl-tRNA synthetases.

    abstract：:The position of the tertiary Levitt pair between nucleotides 15 and 48 in the transfer RNA core region suggests a key role in stabilizing the joining of the two helical domains, and in maintaining the relative orientations of the D and variable loops. E. coli tRNA(Gln) possesses the canonical Pu15-Py48 trans pairing a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3749

    authors： Sherlin LD,Bullock TL,Newberry KJ,Lipman RS,Hou YM,Beijer B,Sproat BS,Perona JJ

    更新日期：2000-06-02 00:00:00

  • Catalytic antibody model and mutagenesis implicate arginine in transition-state stabilization.

    abstract：:To probe the mechanism of the catalytic antibody NPN43C9, we have constructed a three-dimensional model of the NPN43C9 variable region using our antibody structural database (ASD), which takes maximal advantage of immunoglobulin sequence and structural information. The ASD contains separately superimposed variable lig...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1060

    authors： Roberts VA,Stewart J,Benkovic SJ,Getzoff ED

    更新日期：1994-01-21 00:00:00

  • How thioredoxin can reduce a buried disulphide bond.

    abstract：:We present a study of the interaction between thioredoxin and the model enzyme pI258 arsenate reductase (ArsC) from Staphylococcus aureus. ArsC catalyses the reduction of arsenate to arsenite. Three redox active cysteine residues (Cys10, Cys82 and Cys89) are involved. After a single catalytic arsenate reduction event,...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.04.016

    authors： Messens J,Van Molle I,Vanhaesebrouck P,Limbourg M,Van Belle K,Wahni K,Martins JC,Loris R,Wyns L

    更新日期：2004-06-04 00:00:00

  • GTP-dependent conformational changes associated with the functional switch between Galpha and cross-linking activities in brain-derived tissue transglutaminase.

    abstract：:GTP and Ca2+, two well-known modulators of intracellular signaling pathways, control a structural/functional switch between two vital and mutually exclusive activities, cross-linking and Galpha activity, in the same enzyme. The enzyme, a brain-derived tissue-type transglutaminase (TGase), was recently cloned by us in ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2052

    authors： Monsonego A,Friedmann I,Shani Y,Eisenstein M,Schwartz M

    更新日期：1998-10-02 00:00:00

  • The Isoniazid Paradigm of Killing, Resistance, and Persistence in Mycobacterium tuberculosis.

    abstract：:Isoniazid (INH) was the first synthesized drug that mediated bactericidal killing of the bacterium Mycobacterium tuberculosis, a major clinical breakthrough. To this day, INH remains a cornerstone of modern tuberculosis (TB) chemotherapy. This review describes the serendipitous discovery of INH, its effectiveness on T...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2019.02.016

    authors： Vilchèze C,Jacobs WR Jr

    更新日期：2019-08-23 00:00:00

  • Complete assignment of the 1H nuclear magnetic resonance spectrum of French bean plastocyanin. Sequential resonance assignments, secondary structure and global fold.

    abstract：:Sequence-specific proton nuclear magnetic resonance (n.m.r.) assignments for all 99 amino acid residues of French bean Cu(I) plastocyanin are described. The assignments were made using standard sequential assignment procedures and were greatly facilitated by the availability of complete spin system assignments. The ch...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90291-4

    authors： Chazin WJ,Wright PE

    更新日期：1988-08-05 00:00:00

  • Crystallization of p68 on lipid monolayers and as three-dimensional single crystals.

    abstract：:Two-dimensional crystals of p68, a Ca2+ -binding protein that has homology with members of the lipocortin/calpactin family, were obtained by interaction with a phospholipid monolayer. By measuring surface pressure at constant surface area, p68 was found to interact in a Ca2+ -dependent manner specifically with phospha...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90534-2

    authors： Newman R,Tucker A,Ferguson C,Tsernoglou D,Leonard K,Crumpton MJ

    更新日期：1989-03-05 00:00:00

  • Contribution of hydration to protein folding thermodynamics. I. The enthalpy of hydration.

    abstract：:The enthalpy of hydration of polar and non-polar groups upon protein unfolding has been estimated for four globular proteins in the temperature range 5 to 125 degrees C, using structural information on the groups in these proteins exposed to water in the native and unfolded states and volume-corrected calorimetric inf...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1416

    authors： Makhatadze GI,Privalov PL

    更新日期：1993-07-20 00:00:00

  • Detection of single-stranded DNA in scrapie-infected brain by electron microscopy.

    abstract：:The nucleic acid content of enriched preparations of mitochondria/tubulofilamentous particles from normal and scrapie-infected hamster brains were examined by electron microscopy. After spreading on collodion-coated grids circular molecules of approximately 15.7 kb corresponding in size to mitochondrial DNA (mtDNA) we...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90369-W

    authors： Narang HK

    更新日期：1990-12-05 00:00:00

  • Tau Modulates VGluT1 Expression.

    abstract：:Tau displacement from microtubules is the first step in the onset of tauopathies and is followed by toxic protein aggregation. However, other non-canonical functions of Tau might have a role in these pathologies. Here, we demonstrate that a small amount of Tau localizes in the nuclear compartment and accumulates in bo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.01.023

    authors： Siano G,Varisco M,Caiazza MC,Quercioli V,Mainardi M,Ippolito C,Cattaneo A,Di Primio C

    更新日期：2019-02-15 00:00:00

  • Segregation of type I collagen homo- and heterotrimers in fibrils.

    abstract：:Normal type I collagen is a heterotrimer of two alpha1(I) and one alpha2(I) chains, but various genetic and environmental factors result in synthesis of homotrimers that consist of three alpha1(I) chains. The homotrimers completely replace the heterotrimers only in rare recessive disorders. In the general population, ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.08.008

    authors： Han S,McBride DJ,Losert W,Leikin S

    更新日期：2008-10-31 00:00:00

  • Binding mode prediction for a flexible ligand in a flexible pocket using multi-conformation simulated annealing pseudo crystallographic refinement.

    abstract：:We describe multi-conformation simulated annealing-pseudo-crystallographic refinement (MCSA-PCR), a technique developed for predicting the binding mode of a flexible ligand in a flexible binding pocket. To circumvent the local-minimum problem efficiently, this method performs multiple independent cycles of simulated a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5147

    authors： Ota N,Agard DA

    更新日期：2001-11-30 00:00:00

  • Zinc binding catalytic domain of human tankyrase 1.

    abstract：:Tankyrases are recently discovered proteins implicated in many important functions in the cell including telomere homeostasis and mitosis. Tankyrase modulates the activity of target proteins through poly(ADP-ribosyl)ation, and here we report the structure of the catalytic poly(ADP-ribose) polymerase (PARP) domain of h...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.03.058

    authors： Lehtiö L,Collins R,van den Berg S,Johansson A,Dahlgren LG,Hammarström M,Helleday T,Holmberg-Schiavone L,Karlberg T,Weigelt J

    更新日期：2008-05-23 00:00:00

  • Analysis and prediction of functional sub-types from protein sequence alignments.

    abstract：:The increasing number and diversity of protein sequence families requires new methods to define and predict details regarding function. Here, we present a method for analysis and prediction of functional sub-types from multiple protein sequence alignments. Given an alignment and set of proteins grouped into sub-types ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4036

    authors： Hannenhalli SS,Russell RB

    更新日期：2000-10-13 00:00:00

  • A 50th order reaction predicted and observed for sickle hemoglobin nucleation.

    abstract：:Sickle hemoglobin polymerization exhibits a striking sensitivity to initial concentration, with characteristic reaction times that exhibit 30th power dependence on concentration. This extraordinary reaction order is encompassed by a novel double nucleation mechanism that predicts 50th power dependence of the homogeneo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0079

    authors： Cao Z,Ferrone FA

    更新日期：1996-02-23 00:00:00