当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Detection of single-stranded DNA in scrapie-infected brain by electron microscopy.

Detection of single-stranded DNA in scrapie-infected brain by electron microscopy.

Abstract：

:The nucleic acid content of enriched preparations of mitochondria/tubulofilamentous particles from normal and scrapie-infected hamster brains were examined by electron microscopy. After spreading on collodion-coated grids circular molecules of approximately 15.7 kb corresponding in size to mitochondrial DNA (mtDNA) were observed both in normal and scrapie-infected brains. In nucleic acid preparations from scrapie-infected brains multimeric mtDNA and single-stranded DNA strands of about 0.49 X 10(6) daltons were also visualized. These findings demonstrate the presence of a single-stranded DNA in scrapie-infected brains and are consistent with previous data based on enzyme digestion of nucleic acids isolated from scrapie-infected brains.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Narang HK

doi

10.1016/0022-2836(90)90369-W

subject

Has Abstract

pub_date

1990-12-05 00:00:00

pages

469-73

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(90)90369-W

journal_volume

216

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Snapshots of RecA protein involving movement of the C-domain and different conformations of the DNA-binding loops: crystallographic and comparative analysis of 11 structures of Mycobacterium smegmatis RecA.

    abstract：:Mycobacterium smegmatis RecA and its nucleotide complexes crystallize in three different, but closely related, forms characterized by specific ranges of unit cell dimensions. The six crystals reported here and five reported earlier, all grown under the same or very similar conditions, belong to these three forms, all ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.01.058

    authors： Krishna R,Prabu JR,Manjunath GP,Datta S,Chandra NR,Muniyappa K,Vijayan M

    更新日期：2007-04-06 00:00:00

  • Local rather than global folding enables the lead-dependent activity of the 8-17 deoxyribozyme: evidence from contact photo-crosslinking.

    abstract：:The 8-17 deoxyribozyme is an in vitro selected enzyme capable of sequence-specific cleavage of RNA. While selected to be a magnesium and zinc-utilizing enzyme, the 8-17 DNAzyme has been shown to utilize lead for its catalysis. Fluorescence-based experiments have indicated that the magnesium- and zinc-utilizing version...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.11.028

    authors： Liu Y,Sen D

    更新日期：2010-01-15 00:00:00

  • An on-pathway hidden intermediate and the early rate-limiting transition state of Rd-apocytochrome b562 characterized by protein engineering.

    abstract：:The folding pathway of Rd-apocytochrome b562, a four-helix bundle protein, was characterized using Trp and Ala/Gly pair mutations. We found that the Trp mutants (F65W) of both the fully folded Rd-apocytochrome b562 and a partially unfolded intermediate with the N-terminal helix (helix I) unfolded, fold with identical ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.07.057

    authors： Zhou Z,Huang Y,Bai Y

    更新日期：2005-09-30 00:00:00

  • An EM view of the FokI synaptic complex by single particle analysis.

    abstract：:FokI is a type IIS restriction endonuclease that recognizes the 5'-GGATG-3' sequence and cleaves non-specifically at 9 and 13 base-pairs away on the top and bottom strands, respectively, to produce a 5' overhang. FokI is a bipartite endonuclease with separate recognition and cleavage domains. Because of its bipartite ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.04.066

    authors： Vanamee ES,Berriman J,Aggarwal AK

    更新日期：2007-07-06 00:00:00

  • Site-directed mutants of RTP of Bacillus subtilis and the mechanism of replication fork arrest.

    abstract：:DNA replication fork arrest during the termination phase of chromosome replication in Bacillus subtilis is brought about by the replication terminator protein (RTP) bound to specific DNA terminator sequences (Ter sites) distributed throughout the terminus region. An attractive suggestion by others was that crucial to ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2553

    authors： Duggin IG,Andersen PA,Smith MT,Wilce JA,King GF,Wake RG

    更新日期：1999-03-12 00:00:00

  • Isolation of kasugamycin resistant mutants in the 16 S ribosomal RNA of Escherichia coli.

    abstract：:Three ribosomal RNA mutations conferring resistance to the antibiotic kasugamycin were isolated using a strain of Escherichia coli in which all of the rRNA is transcribed from a plasmid-encoded rrn operon. The mutations, A794G, G926A, and A1519C, mapped to universally conserved sites in the 16 S RNA gene. Site-directe...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3160

    authors： Vila-Sanjurjo A,Squires CL,Dahlberg AE

    更新日期：1999-10-15 00:00:00

  • Selection of Streptomyces griseus protease B mutants with desired alterations in primary specificity using a library screening strategy.

    abstract：:Streptomyces griseus protease B (SGPB) has primary specificity for large hydrophobic residues. The protease is secreted in a promature form, and autocatalytic removal of the propeptide is essential for activity. We genetically substituted the P1 Leu at the promature junction of SGPB with Phe, Met, or Val and monitored...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0159

    authors： Sidhu SS,Borgford TJ

    更新日期：1996-03-29 00:00:00

  • Crystal structure of shikimate kinase from Mycobacterium tuberculosis reveals the dynamic role of the LID domain in catalysis.

    abstract：:Shikimate kinase (SK) and other enzymes in the shikimate pathway are potential targets for developing non-toxic antimicrobial agents, herbicides, and anti-parasite drugs, because the pathway is essential in the above species but is absent from mammals. The crystal structure of Mycobacterium tuberculosis SK (MtSK) in c...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00339-X

    authors： Gu Y,Reshetnikova L,Li Y,Wu Y,Yan H,Singh S,Ji X

    更新日期：2002-06-07 00:00:00

  • Engineering activity and stability of Thermotoga maritima glutamate dehydrogenase. II: construction of a 16-residue ion-pair network at the subunit interface.

    abstract：:The role of an 18-residue ion-pair network, that is present in the glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus, in conferring stability to other, less stable homologous enzymes, has been studied by introducing four new charged amino acid residues into the subunit interface of glutam...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2779

    authors： Lebbink JH,Knapp S,van der Oost J,Rice D,Ladenstein R,de Vos WM

    更新日期：1999-06-04 00:00:00

  • Structural basis of substrate specificity and selectivity of murine cytosolic 5'-nucleotidase III.

    abstract：:Cytosolic 5'-nucleotidase III (cN-III) is responsible for selective degradation of pyrimidine 5'-monoribonucleotides during maturation of reticulocytes to erythrocytes. The lack of this enzymatic activity due to genetic aberrations or lead poisoning results in a mild to moderate nonspherocytic hemolytic anemia. In aff...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.08.014

    authors： Grobosky CL,Lopez JB,Rennie S,Skopelitis DJ,Wiest AT,Bingman CA,Bitto E

    更新日期：2012-11-02 00:00:00

  • Probing myosin head structure with monoclonal antibodies.

    abstract：:Monoclonal antibodies that react with defined regions of the heavy and light chains of chicken skeletal muscle myosin have been used to provide a correlation between the primary and the tertiary structures of the head. Electron microscopy of rotary shadowed antibody-myosin complexes shows that the sites for three epit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(86)80009-2

    authors： Winkelmann DA,Lowey S

    更新日期：1986-04-20 00:00:00

  • Crystal structure of the motor domain of the human kinetochore protein CENP-E.

    abstract：:The human kinetochore is a highly complex macromolecular structure that connects chromosomes to spindle microtubules (MTs) in order to facilitate accurate chromosome segregation. Centromere-associated protein E (CENP-E), a member of the kinesin superfamily, is an essential component of the kinetochore, since it is req...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.05.053

    authors： Garcia-Saez I,Yen T,Wade RH,Kozielski F

    更新日期：2004-07-23 00:00:00

  • Structural comparison of cuticle and interstitial collagens from annelids living in shallow sea-water and at deep-sea hydrothermal vents.

    abstract：:Two types of annelid collagens of different sizes were purified, one from acetic acid extracts of the cuticle (length 2.5 microns) and the other, after pepsin digestion, from interstitial spaces of the body wall (0.3 micron). They were obtained from Alvinella pompejana, Alvinella caudata and Paralvinella grasslei coll...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.0084

    authors： Gaill F,Mann K,Wiedemann H,Engel J,Timpl R

    更新日期：1995-02-17 00:00:00

  • Site-directed mutagenesis on human cystathionine-gamma-lyase reveals insights into the modulation of H2S production.

    abstract：:In recent years, increased interest has been directed towards hydrogen sulfide (H2S) as the third gasotransmitter and its role in various diseases. Cystathionine-gamma-lyase (CSE) is one of the enzymes responsible for the endogenous production of H2S in mammals. With the aid of the crystal structures of human CSE and ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.11.058

    authors： Huang S,Chua JH,Yew WS,Sivaraman J,Moore PK,Tan CH,Deng LW

    更新日期：2010-02-26 00:00:00

  • Stochastic switching in gene networks can occur by a single-molecule event or many molecular steps.

    abstract：:Due to regulatory feedback, biological networks can exist stably in multiple states, leading to heterogeneous phenotypes among genetically identical cells. Random fluctuations in protein numbers, tuned by specific molecular mechanisms, have been hypothesized to drive transitions between these different states. We deve...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.11.035

    authors： Choi PJ,Xie XS,Shakhnovich EI

    更新日期：2010-02-12 00:00:00

  • Membrane structure of CtrA3, a copper-transporting P-type-ATPase from Aquifex aeolicus.

    abstract：:We have produced and characterized two new copper-transporting ATPases, CtrA2 and CtrA3 from Aquifex aeolicus, that belong to the family of heavy metal ion-transporting P(IB)-type ATPases. CtrA2 has a CPC metal-binding sequence in TM6 and a CxxC metal-binding N-terminal domain, while CtrA3 has a CPH metal-binding moti...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.01.094

    authors： Chintalapati S,Al Kurdi R,van Scheltinga AC,Kühlbrandt W

    更新日期：2008-05-02 00:00:00

  • Molecular determinants for substrate specificity of the ligand-binding protein OpuAC from Bacillus subtilis for the compatible solutes glycine betaine and proline betaine.

    abstract：:Compatible solutes play a decisive role in the defense of microorganisms against changes in temperature and increases in osmolarity in their natural habitats. In Bacillus subtilis, the substrate-binding protein (SBP)-dependent ABC-transporter OpuA serves for the uptake of the compatible solutes glycine betaine (GB) an...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.12.085

    authors： Horn C,Sohn-Bösser L,Breed J,Welte W,Schmitt L,Bremer E

    更新日期：2006-03-24 00:00:00

  • Polypyrimidine tract-binding protein inhibits translation of bip mRNA.

    abstract：:Translation initiation of human Bip mRNA is directed by an internal ribosomal entry site (IRES) located in the 5' non-translated region. No trans-acting factor possibly involved in this process has as of yet been identified. For the encephalomyocarditis virus and other picornaviruses, polypyrimidine tract-binding prot...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4179

    authors： Kim YK,Hahm B,Jang SK

    更新日期：2000-11-24 00:00:00

  • From dusk till dawn: one-plasmid systems for light-regulated gene expression.

    abstract：:Signaling photoreceptors mediate diverse organismal adaptations in response to light. As light-gated protein switches, signaling photoreceptors provide the basis for optogenetics, a term that refers to the control of organismal physiology and behavior by light. We establish as novel optogenetic tools the plasmids pDus...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.01.001

    authors： Ohlendorf R,Vidavski RR,Eldar A,Moffat K,Möglich A

    更新日期：2012-03-02 00:00:00

  • Structural characterization of protein-protein complexes by integrating computational docking with small-angle scattering data.

    abstract：:X-ray crystallography and NMR can provide detailed structural information of protein-protein complexes, but technical problems make their application challenging in the high-throughput regime. Other methods such as small-angle X-ray scattering (SAXS) are more promising for large-scale application, but at the cost of l...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.08.029

    authors： Pons C,D'Abramo M,Svergun DI,Orozco M,Bernadó P,Fernández-Recio J

    更新日期：2010-10-22 00:00:00

  • Structural studies of HIV-1 Tat protein.

    abstract：:Tat (trans-activator) proteins are early RNA binding proteins regulating lentiviral transcription. These proteins are necessary components in the life cycle of all known lentiviruses, such as the human immunodeficiency viruses (HIV) or the equine infectious anemia virus (EIAV). Tat proteins are thus ideal targets for ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0158

    authors： Bayer P,Kraft M,Ejchart A,Westendorp M,Frank R,Rösch P

    更新日期：1995-04-07 00:00:00

  • Stalling of human DNA (cytosine-5) methyltransferase at single-strand conformers from a site of dynamic mutation.

    abstract：:Single-strand conformers (SSCs) from the C-rich strand of the triplet repeat at the FMR-1 locus are rapidly and selectively methylated by the human DNA (cytosine-5) methyltransferase. The apparent affinity of the enzyme for the FMR-1 SSC is about tenfold higher than it is for a control Watson-Crick paired duplex. The ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1430

    authors： Kho MR,Baker DJ,Laayoun A,Smith SS

    更新日期：1998-01-09 00:00:00

  • Yeast amber suppressors corresponding to tRNA3Leu genes.

    abstract：:Amber suppressors previously isolated from the yeast Saccharomyces cerevisiae and belonging to the same phenotypic class (Liebman et al., 1976) were assigned to nine different linkage groups named SUP52 through SUP60. One of these suppressors, SUP52, had been shown to cause the insertion of leucine and had been geneti...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90140-2

    authors： Liebman SW,Srodulski Z,Reed CR,Stewart JW,Sherman F,Brennan G

    更新日期：1984-09-15 00:00:00

  • NMR Investigation of the Interaction between the RecQ C-Terminal Domain of Human Bloom Syndrome Protein and G-Quadruplex DNA from the Human c-Myc Promoter.

    abstract：:Bloom syndrome protein (BLM) is one of five human RecQ helicases that participate in DNA metabolism. RecQ C-terminal (RQC) domain is the main DNA binding module of BLM and specifically recognizes G-quadruplex (G4) DNA structures. Because G4 processing by BLM is essential for regulating replication and transcription, b...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.01.010

    authors： Lee S,Lee AR,Ryu KS,Lee JH,Park CJ

    更新日期：2019-02-15 00:00:00

  • Secondary structure constraints on the evolution of Drosophila 28 S ribosomal RNA expansion segments.

    abstract：:Eukaryotic ribosomal RNA genes contain rapidly evolving regions of unknown function termed expansion segments. We present the comparative analysis of the primary and secondary structure of two expansion segments from the large subunit rRNA gene of ten species of Drosophila and the tsetse fly species Glossina morsitans...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90178-9

    authors： Ruiz Linares A,Hancock JM,Dover GA

    更新日期：1991-06-05 00:00:00

  • The central PPT of the yeast retrotransposon Ty1 is not essential for transposition.

    abstract：:The yeast retrotransposon Ty1 has structural and functional similarities to retroviruses. We report here that, as in retroviruses, the plus-strand DNA of Ty1 is synthesized as two segments. A central DNA flap is formed during reverse transcription consecutive to elongation (with strand displacement) of the upstream se...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00812-x

    authors： Heyman T,Wilhelm M,Wilhelm FX

    更新日期：2003-08-08 00:00:00

  • Ro ribonucleoprotein assembly in vitro. Identification of RNA-protein and protein-protein interactions.

    abstract：:The human Y RNAs, small RNAs with an unknown function, are complexed with at least three proteins: the 60,000 M(r) Ro protein (Ro60), the 52,000 M(r) Ro protein (Ro52) and the La protein (La). In this study we examined the intermolecular interactions between the components of these so-called Ro ribonucleoprotein (Ro R...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90890-v

    authors： Slobbe RL,Pluk W,van Venrooij WJ,Pruijn GJ

    更新日期：1992-09-20 00:00:00

  • Guided evolution of enzymes with new substrate specificities.

    abstract：:A gene library was constructed coding for all possible variants of two amino acids (101, 102) in a solvent-exposed surface return loop (alpha E-beta D) of Bacillus stearothermophilus L-lactate dehydrogenase (bsLDH). All but one of 38 enzyme variants examined were thermally stable and had native-like hydrodynamic prope...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0626

    authors： el Hawrani AS,Sessions RB,Moreton KM,Holbrook JJ

    更新日期：1996-11-22 00:00:00

  • The C-terminal lysine of Ogg2 DNA glycosylases is a major molecular determinant for guanine/8-oxoguanine distinction.

    abstract：:7,8-Dihydro-8-oxoguanine (8-oxoG) is a major oxidative lesion found in DNA. The 8-oxoguanine DNA glycosylases (Ogg) responsible for the removal of 8-oxoG are divided into three families Ogg1, Ogg2 and AGOG. The Ogg2 members are devoid of the recognition loop used by Ogg1 to discriminate between 8-oxoG and guanine and ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.01.024

    authors： Faucher F,Wallace SS,Doublié S

    更新日期：2010-03-19 00:00:00

  • Domain 1.1 of the sigma(70) subunit of Escherichia coli RNA polymerase modulates the formation of stable polymerase/promoter complexes.

    abstract：:The sigma 70 (sigma(70)) subunit of Escherichia coli RNA polymerase specifies transcription from promoters that are responsible for basal gene expression during vegetative growth. When sigma(70) is present within polymerase holoenzyme, two of its domains, 2.4 and 4.2, interact with sequences within the -10 and -35 reg...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4690

    authors： Vuthoori S,Bowers CW,McCracken A,Dombroski AJ,Hinton DM

    更新日期：2001-06-08 00:00:00