Abstract：
:The yeast retrotransposon Ty1 has structural and functional similarities to retroviruses. We report here that, as in retroviruses, the plus-strand DNA of Ty1 is synthesized as two segments. A central DNA flap is formed during reverse transcription consecutive to elongation (with strand displacement) of the upstream segment beyond the central polypurine tract (cPPT) until the replication machinery is stopped at the central termination sequence. Comparison of wild-type and cPPT-mutant Ty1 elements shows that the mutant element lacking the central DNA flap is only twofold defective in transposition.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Heyman T,Wilhelm M,Wilhelm FXdoi
10.1016/s0022-2836(03)00812-xkeywords:
subject
Has Abstractpub_date
2003-08-08 00:00:00pages
315-20issue
2eissn
0022-2836issn
1089-8638pii
S002228360300812Xjournal_volume
331pub_type
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