Abstract:
:The SH3 domain is a protein-protein interaction module commonly found in intracellular signaling and adaptor proteins. The SH3 domains of multiple endocytic proteins have been recently implicated in binding ubiquitin, which serves as a signal for diverse cellular processes including gene regulation, endosomal sorting, and protein destruction. Here we describe the solution NMR structure of ubiquitin in complex with an SH3 domain belonging to the yeast endocytic protein Sla1. The ubiquitin binding surface of the Sla1 SH3 domain overlaps substantially with the canonical binding surface for proline-rich ligands. Like many other ubiquitin-binding motifs, the SH3 domain engages the Ile44 hydrophobic patch of ubiquitin. A phenylalanine residue located at the heart of the ubiquitin-binding surface of the SH3 domain serves as a key specificity determinant. The structure of the SH3-ubiquitin complex explains how a subset of SH3 domains has acquired this non-traditional function.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
He Y,Hicke L,Radhakrishnan Idoi
10.1016/j.jmb.2007.07.074subject
Has Abstractpub_date
2007-10-12 00:00:00pages
190-6issue
1eissn
0022-2836issn
1089-8638pii
S0022-2836(07)01052-2journal_volume
373pub_type
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