A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA.

abstract：:The structure of the yeast DNA-dependent RNA polymerase I (RNA Pol I), prepared by cryo-negative staining, was studied by electron microscopy. A structural model of the enzyme at a resolution of 1.8 nm was determined from the analysis of isolated molecules and showed an excellent fit with the atomic structure of the R...

journal_title：Journal of molecular biology

authors： De Carlo S,Carles C,Riva M,Schultz P

更新日期：2003-06-20 00:00:00

abstract：:We have probed the structure of the human mitochondrial DNA helicase, an enzyme that uses the energy of nucleotide hydrolysis to unwind duplex DNA during mitochondrial DNA replication. This novel helicase shares substantial amino acid sequence and functional similarities with the bacteriophage T7 primase-helicase. We ...

journal_title：Journal of molecular biology

authors： Ziebarth TD,Farr CL,Kaguni LS

更新日期：2007-04-13 00:00:00

abstract：:The average structural and dynamic properties of tetragonal hen egg-white lysozyme have been compared, in structures refined at 1.9 A resolution, using data collected at 100 K and 298 K. The molecule expands by 1.8% over this temperature range with the expansion occurring primarily in its small sub-atomic-sized spaces...

journal_title：Journal of molecular biology

authors： Young AC,Tilton RF,Dewan JC

更新日期：1994-01-07 00:00:00

abstract：:The tyrosyl-tRNA synthetases from Bacillus stearothermophilus (Bst-TyrTS) and Escherichia coli (Eco-TyrTS) are 56% identical in amino acid sequence. To map and characterize the set of interactions that makes Bst-TyrTS more stable than Eco-TyrTS, a family of nine hybrid proteins was constructed between the two enzymes....

journal_title：Journal of molecular biology

authors： Guez-Ivanier V,Hermann M,Baldwin D,Bedouelle H

更新日期：1993-11-05 00:00:00

abstract：:Tissue deposition of normally soluble proteins as insoluble amyloid fibrils is associated with serious diseases including the systemic amyloidoses, maturity onset diabetes, Alzheimer's disease and transmissible spongiform encephalopathy. Although the precursor proteins in different diseases do not share sequence homol...

journal_title：Journal of molecular biology

authors： Sunde M,Serpell LC,Bartlam M,Fraser PE,Pepys MB,Blake CC

更新日期：1997-10-31 00:00:00

abstract：:The structure of filamentous bacteriophage Pf1 has been studied using neutron diffraction from magnetically oriented gels of native and valine-deuterated phage. Neutron diffraction intensities were measured to approximately 8 A resolution along the equator and first six layer-lines, and differences due to the deuterat...

journal_title：Journal of molecular biology

authors： Stark W,Glucksman MJ,Makowski L

更新日期：1988-01-05 00:00:00

abstract：:The nuclear hormone receptor peroxisome proliferator-activated receptor gamma (PPAR gamma; NR1C3) plays a central role in adipogenesis and is the molecular target of the thiazolidinedione class of antidiabetic drugs. To overcome the well-known shortcomings of thiazolidinediones, we have identified INT131 (formerly T13...

journal_title：Journal of molecular biology

authors： Motani A,Wang Z,Weiszmann J,McGee LR,Lee G,Liu Q,Staunton J,Fang Z,Fuentes H,Lindstrom M,Liu J,Biermann DH,Jaen J,Walker NP,Learned RM,Chen JL,Li Y

更新日期：2009-03-13 00:00:00

abstract：:The detection of conserved sequence patterns (motifs) in related proteins often yields valuable structural and functional insights. We describe a method that utilizes rigorous statistics and a depth-first search procedure to efficiently and exhaustively search a set of proteins for significant patterns up to a specifi...

journal_title：Journal of molecular biology

authors： Neuwald AF,Green P

更新日期：1994-06-24 00:00:00

abstract：:The backbone dynamics of the EF-hand Ca(2+)-binding protein, calbindin D9k, has been investigated in the apo, (Cd2+)1 and (Ca2+)2 states by measuring the rate constants for amide proton exchange with solvent. 15N-1H correlation spectroscopy was utilized to follow direct 1H-->2H exchange of the slowly exchanging amide ...

journal_title：Journal of molecular biology

authors： Skelton NJ,Kördel J,Akke M,Chazin WJ

更新日期：1992-10-20 00:00:00

abstract：:Cross-links in tendon collagen are essential for the biomechanical strength of healthy tissue. The nature and position of these cross-links has long been a subject for conjecture. We have approached this problem in a non-destructive manner, by studying neutron diffraction from collagen fibrils that have been specifica...

journal_title：Journal of molecular biology

authors： Wess TJ,Miller A,Bradshaw JP

更新日期：1990-05-05 00:00:00

abstract：:The crystal structure of the staphylococcal nuclease mutant V66K, in which valine 66 is replaced by lysine, has been solved at 1.97 A resolution. Unlike lysine residues in previously reported protein structures, this residue appears to bury its side-chain in the hydrophobic core without salt bridging, hydrogen bonding...

journal_title：Journal of molecular biology

authors： Stites WE,Gittis AG,Lattman EE,Shortle D

更新日期：1991-09-05 00:00:00

abstract：:Pathogenic strains of Helicobacter pylori use a type IV secretion system (T4SS) to deliver the toxin CagA into human host cells. The T4SS, along with the toxin itself, is coded into a genomic insert, which is termed the cag pathogenicity island. The cag pathogenicity island contains about 30 open-reading frames, for m...

journal_title：Journal of molecular biology

authors： Cendron L,Couturier M,Angelini A,Barison N,Stein M,Zanotti G

更新日期：2009-02-13 00:00:00

abstract：:Using a clone characterized in the course of a random sequencing programme of Arabidopsis thaliana, two cDNAs encoding plant type cytosolic NADPH-dependent thioredoxin reductase (NTR) have been isolated. Their sequence homology with Escherichia coli NRT (the only thioredoxin reductase of known primary structure) is ab...

journal_title：Journal of molecular biology

authors： Jacquot JP,Rivera-Madrid R,Marinho P,Kollarova M,Le Maréchal P,Miginiac-Maslow M,Meyer Y

更新日期：1994-01-28 00:00:00

abstract：:We present the first large-scale survey of N-terminal protein maturation in archaea based on 873 proteomically identified N-terminal peptides from the two haloarchaea Halobacterium salinarum and Natronomonas pharaonis. The observed protein maturation pattern can be attributed to the combined action of methionine amino...

journal_title：Journal of molecular biology

authors： Falb M,Aivaliotis M,Garcia-Rizo C,Bisle B,Tebbe A,Klein C,Konstantinidis K,Siedler F,Pfeiffer F,Oesterhelt D

更新日期：2006-10-06 00:00:00

abstract：:We have identified the recognition sequence for the Citrobacter freundii restriction endonuclease CfrA, a member of the A-family of type I R-M enzymes. This bipartite target sequence differs in both its components from those of other type I enzymes. We determined the nucleotide sequence of its specificity gene (hsdS) ...

journal_title：Journal of molecular biology

authors： Kannan P,Cowan GM,Daniel AS,Gann AA,Murray NE

更新日期：1989-10-05 00:00:00

abstract：:The effects of regulatory amounts of Ca2+ on the in situ structures of troponin C (TnC) and troponin I (TnI) in whole troponin have been investigated by neutron scattering. In separate difference experiments, 97% deuterated TnC and TnI within whole troponin were studied +/-Ca2+ in 41.6% 2H2O buffers in which protonate...

journal_title：Journal of molecular biology

authors： Stone DB,Timmins PA,Schneider DK,Krylova I,Ramos CH,Reinach FC,Mendelson RA

更新日期：1998-08-28 00:00:00

abstract：:The crystal structure of desulforedoxin from Desulfovibrio gigas, a new homo-dimeric (2 x 36 amino acids) non-heme iron protein, has been solved by the SIRAS method using the indium-substituted protein as the single derivative. The structure was refined to a crystallographic R-factor of 16.9% at 1.8 A resolution. Nati...

journal_title：Journal of molecular biology

authors： Archer M,Huber R,Tavares P,Moura I,Moura JJ,Carrondo MA,Sieker LC,LeGall J,Romão MJ

更新日期：1995-09-01 00:00:00

abstract：:Type IV pili are multifunctional filaments displayed on many bacterial pathogens. Members of the Type IVa pilus subclass are found on a diverse group of human pathogens, whereas Type IVb pili are found almost exclusively on enteric bacteria. The Type IVa and IVb subclasses are distinguished by differences in the pilin...

journal_title：Journal of molecular biology

authors： Li J,Egelman EH,Craig L

更新日期：2012-04-20 00:00:00

abstract：:Crystalline complexes of tyrosyl-tRNA synthetase from Bacillus stearothermophilus were prepared with adenosine, AMP, ATP and PPi, all in the presence of tyrosinol, which binds strongly to the tyrosine binding site but cannot be adenylated by ATP. The hydrolysis of ATP in the presence of crystalline tyrosyl-tRNA synthe...

journal_title：Journal of molecular biology

authors： Monteilhet C,Blow DM,Brick P

更新日期：1984-03-15 00:00:00

abstract：:We have identified independently folding units, so called "foldons", from non-homologous proteins representing different folds. We applied simple statistical arguments in order to estimate the size of the foldon universe required to construct all foldable proteins. Various alignment procedures yield about 2600 foldons...

journal_title：Journal of molecular biology

authors： Panchenko AR,Luthey-Schulten Z,Cole R,Wolynes PG

更新日期：1997-09-12 00:00:00

abstract：:A full length (25,000 base-pair) myosin heavy chain gene completely contained within a single cosmid clone was isolated from a Syrian hamster cosmid genomic library. Sequence comparison of the 3' untranslated region indicated the presence of a 75% homology with the rat embryonic myosin heavy chain gene. Extensive 5' f...

journal_title：Journal of molecular biology

authors： McCully JD,Wang RX,Kellam B,Sole MJ,Liew CC

更新日期：1991-04-20 00:00:00

abstract：:A specific complex of 5 S rRNA and several ribosomal proteins is an integral part of ribosomes in all living organisms. Here we studied the importance of Escherichia coli genes rplE, rplR and rplY, encoding 5 S rRNA-binding ribosomal proteins L5, L18 and L25, respectively, for cell growth, viability and translation. U...

journal_title：Journal of molecular biology

authors： Korepanov AP,Gongadze GM,Garber MB,Court DL,Bubunenko MG

更新日期：2007-03-02 00:00:00

abstract：:Yeast proteinase B inhibitor 2 (YIB2), which is composed of 74 amino acid residues, is an unusual serine protease inhibitor, since it lacks disulfide bonds. To identify its reactive site for proteases, we constructed an expression system for a synthetic YIB2 gene and then attempted to change the inhibitory properties ...

journal_title：Journal of molecular biology

authors： Kojima S,Deguchi M,Miura K

更新日期：1999-02-26 00:00:00

abstract：:Sequences flanking the protein-coding regions of 15 of the 17 to 20 actin genes in the cellular slime mold Dictyostelium discoidium have been determined. Comparison of sequences among genes shows that they contain extensive homologies at both the 5' and 3' ends of the coding regions. On the basis of these homologies, ...

journal_title：Journal of molecular biology

authors： Romans P,Firtel RA

更新日期：1985-06-05 00:00:00

abstract：:We have conducted a detailed kinetic and thermodynamic analysis of open complex formation between Escherichia coli RNA polymerase and the A1 promoter from bacteriophage T7 by monitoring alterations in the intrinsic protein fluorescence of RNA polymerase in stopped-flow kinetic studies. The stopped-flow kinetic data ar...

journal_title：Journal of molecular biology

authors： Johnson RS,Chester RE

更新日期：1998-10-23 00:00:00

abstract：:Colicin endonucleases (DNases) are bound and inactivated by immunity (Im) proteins. Im proteins are broadly cross-reactive yet specific inhibitors binding cognate and non-cognate DNases with K(d) values that vary between 10(-4) and 10(-14) M, characteristics that are explained by a 'dual-recognition' mechanism. In thi...

journal_title：Journal of molecular biology

authors： Keeble AH,Joachimiak LA,Maté MJ,Meenan N,Kirkpatrick N,Baker D,Kleanthous C

更新日期：2008-06-13 00:00:00

abstract：:EF-Tu has been shown to interact with actin-like protein MreB and to affect its localization in Escherichia coli and in Bacillus subtilis cells. We have purified YFP-MreB in an active form, which forms filaments on glass slides in vitro and was active in dynamic light-scattering assays, polymerizing in milliseconds af...

journal_title：Journal of molecular biology

authors： Defeu Soufo HJ,Reimold C,Breddermann H,Mannherz HG,Graumann PL

更新日期：2015-04-24 00:00:00

abstract：:Understanding gene regulation and its adaptive significance requires not only a detailed knowledge of individual molecular interactions that give rise to changes in gene expression but also an overview of complete genetic networks and the ways in which components within them interact. Increasingly, such studies are be...

journal_title：Journal of molecular biology

authors： Burton NA,Johnson MD,Antczak P,Robinson A,Lund PA

更新日期：2010-09-03 00:00:00

abstract：:L-threonine dehydrogenase (TDH) is an enzyme that catalyzes the oxidation of L-threonine to 2-amino-3-ketobutyrate. We solved the first crystal structure of a medium chain L-threonine dehydrogenase from a hyperthermophilic archaeon, Pyrococcus horikoshii (PhTDH), by the single wavelength anomalous diffraction method u...

journal_title：Journal of molecular biology

authors： Ishikawa K,Higashi N,Nakamura T,Matsuura T,Nakagawa A

更新日期：2007-02-23 00:00:00

abstract：:In recent years, increased interest has been directed towards hydrogen sulfide (H2S) as the third gasotransmitter and its role in various diseases. Cystathionine-gamma-lyase (CSE) is one of the enzymes responsible for the endogenous production of H2S in mammals. With the aid of the crystal structures of human CSE and ...

journal_title：Journal of molecular biology

authors： Huang S,Chua JH,Yew WS,Sivaraman J,Moore PK,Tan CH,Deng LW

更新日期：2010-02-26 00:00:00