Conformational changes in the ribosome induced by translational miscoding agents.

abstract：:TWINKLE is the helicase at the mitochondrial DNA (mtDNA) replication fork in mammalian cells. Mutations in the PEO1 gene, which encodes TWINKLE, cause autosomal dominant progressive external ophthalmoplegia (AdPEO), a disorder associated with deletions in mtDNA. Here, we characterized seven different AdPEO-causing mut...

journal_title：Journal of molecular biology

authors： Korhonen JA,Pande V,Holmlund T,Farge G,Pham XH,Nilsson L,Falkenberg M

更新日期：2008-03-28 00:00:00

abstract：:The DNA decamer d(CCAGGCCTGG) has been studied by X-ray crystallography. At a nominal resolution of 1.6 A, the structure was refined to R = 16.9% using stereochemical restraints. The oligodeoxyribonucleotide forms a straight B-DNA double helix with crystallographic dyad symmetry and ten base-pairs per turn. In the cry...

journal_title：Journal of molecular biology

authors： Heinemann U,Alings C

更新日期：1989-11-20 00:00:00

abstract：:Relaxation of nucleosomes on an homologous series (pBR) of ca 350-370 bp DNA minicircles originating from plasmid pBR322 was recently used as a tool to study their structure and dynamics. These nucleosomes thermally fluctuated between three distinct DNA conformations within a histone N-terminal tail-modulated equilibr...

journal_title：Journal of molecular biology

authors： Sivolob A,Lavelle C,Prunell A

更新日期：2003-02-07 00:00:00

abstract：:Historically referred to as "the GTPase center", the L11 binding region (L11BR) of Escherichia coli 23 S rRNA is a highly conserved structure that has been implicated in several essential functions during protein synthesis. Here, in vivo expression of an RNA fragment containing that structure was found to affect trans...

journal_title：Journal of molecular biology

authors： Van Dyke N,Xu W,Murgola EJ

更新日期：2002-05-31 00:00:00

abstract：:We have isolated and characterized the murine genomic and complementary DNAs encoding erythropoietin (Epo) receptor from Epo-responsive and unresponsive mouse erythroleukemia cells. Two classes of Epo receptor cDNAs were isolated from Epo-responsive cells. One is a 55,000 Mr membrane-bound Epo receptor, and the other ...

journal_title：Journal of molecular biology

authors： Kuramochi S,Ikawa Y,Todokoro K

更新日期：1990-12-05 00:00:00

abstract：:Bovine IF(1) is a basic, 84 amino acid residue protein that inhibits the hydrolytic action of the F(1)F(0) ATP synthase in mitochondria under anaerobic conditions. Its oligomerization state is dependent on pH. At a pH value below 6.5 it forms an active dimer. At higher pH values, two dimers associate to form an inacti...

journal_title：Journal of molecular biology

authors： Gordon-Smith DJ,Carbajo RJ,Yang JC,Videler H,Runswick MJ,Walker JE,Neuhaus D

更新日期：2001-04-27 00:00:00

abstract：:Lysyl and prolyl hydroxylations are well-known post-translational modifications to animal and plant proteins with extracellular roles. More recent work has indicated that the hydroxylation of intracellular animal proteins may be common. JMJD6 catalyses the iron- and 2-oxoglutarate-dependent hydroxylation of lysyl resi...

journal_title：Journal of molecular biology

authors： Mantri M,Krojer T,Bagg EA,Webby CJ,Butler DS,Kochan G,Kavanagh KL,Oppermann U,McDonough MA,Schofield CJ

更新日期：2010-08-13 00:00:00

abstract：:Transcription activator-like effectors (TALEs) recognize DNA through repeat-variable diresidues (RVDs), and TALE-DNA interactions are sensitive to DNA modifications. Our previous study deciphered the recognition of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) by TALEs. Here, we report seven crystal struct...

journal_title：Journal of molecular biology

authors： Liu L,Zhang Y,Liu M,Wei W,Yi C,Peng J

更新日期：2020-02-14 00:00:00

abstract：:Intercellular contacts in multicellular organisms are maintained by membrane receptors called cell adhesion molecules (CAMs), which are expressed on cell surfaces. One interesting feature of CAMs is that almost all of their extracellular regions contain repeating copies of structural domains. It is not clear why so ma...

journal_title：Journal of molecular biology

authors： Chen J,Wu Y

更新日期：2017-04-07 00:00:00

abstract：:2a2 is the most commonly rearranged gene in the human V(lambda )locus. It has been postulated that certain immunoglobulin genes (including 2a2) are rearranged preferentially because their germline sequences encode structures capable of binding to a range of antigens. Somatic mutation could then increase the specificit...

journal_title：Journal of molecular biology

authors： Rahman A,Haley J,Radway-Bright E,Nagl S,Low DG,Latchman DS,Isenberg DA

更新日期：2001-03-16 00:00:00

abstract：:Allosteric regulatory processes are implicated at all levels of biological function. Recent advances in our understanding of the diverse and functionally significant class of intrinsically disordered proteins have identified a multitude of ways in which disordered proteins function within the confines of the allosteri...

journal_title：Journal of molecular biology

authors： Berlow RB,Dyson HJ,Wright PE

更新日期：2018-08-03 00:00:00

abstract：:Successful immunotherapy of cocaine addiction and overdoses requires cocaine-binding antibodies with specific properties, such as high affinity and selectivity for cocaine. We have determined the affinities of two cocaine-binding murine monoclonal antibodies (mAb: clones 3P1A6 and MM0240PA) for cocaine and its metabol...

journal_title：Journal of molecular biology

authors： Paula S,Tabet MR,Keenan SM,Welsh WJ,Ball WJ Jr

更新日期：2003-01-17 00:00:00

abstract：:Malate dehydrogenase from the extreme halophile Halobacterium marismortui crystallizes in highly concentrated phosphate solution in space group 12 with cell dimensions a = 113.8 A, b = 122.8 A, c = 126.7 A, beta = 98.1 degrees. The halophilic enzyme was found to be unstable at lower concentrations of phosphate. It ass...

journal_title：Journal of molecular biology

authors： Harel M,Shoham M,Frolow F,Eisenberg H,Mevarech M,Yonath A,Sussman JL

更新日期：1988-04-05 00:00:00

abstract：:A new method is suggested here for topology prediction of helical transmembrane proteins. The method is based on the hypothesis that the localizations of the transmembrane segments and the topology are determined by the difference in the amino acid distributions in various structural parts of these proteins rather tha...

journal_title：Journal of molecular biology

authors： Tusnády GE,Simon I

更新日期：1998-10-23 00:00:00

abstract：:UDP-glucose is the universal activated form of glucose, employed in all organisms for glucosyl transfer reactions and as precursor for various activated carbohydrates. In animal and fungal metabolism, UDP-glucose is required for utilization of galactose and for the synthesis of glycogen, the major carbohydrate storage...

journal_title：Journal of molecular biology

authors： Roeben A,Plitzko JM,Körner R,Böttcher UM,Siegers K,Hayer-Hartl M,Bracher A

更新日期：2006-12-08 00:00:00

abstract：:The 3.0-3.1A X-ray structures of the cytochrome b(6)f complex from Mastigocladus laminosus and Chlamydomonas reinhardtii obtained in the presence of the p-side quinone-analogue inhibitor tridecyl-stigmatellin (TDS) are very similar. A difference occurs in the p-side binding position of TDS. In C.reinhardtii, TDS binds...

journal_title：Journal of molecular biology

authors： Yan J,Cramer WA

更新日期：2004-11-19 00:00:00

abstract：:The Fv fragment from an anti-dansyl antibody was optimally crystallized into two crystal forms having slightly different lattice dimensions at pH 5.25 and 6.75. The two crystal structures were determined and refined at high resolution at 112 K (at 1.45 A for the crystal at pH 5.25 and at 1.55 A for that at pH 6.75). I...

journal_title：Journal of molecular biology

authors： Nakasako M,Takahashi H,Shimba N,Shimada I,Arata Y

更新日期：1999-08-06 00:00:00

abstract：:Like a variety of other bacteriophages, such as T4 and P22, bacteriophage P1 packages DNA by a "headful" mechanism in which the capacity of the viral capsid determines the size of the single DNA molecule that is packaged. Because of the long-standing and general acceptance of this packaging mechanism, we were surprise...

journal_title：Journal of molecular biology

authors： Coren JS,Pierce JC,Sternberg N

更新日期：1995-05-26 00:00:00

abstract：:The site-specific binding interaction of lac repressor with a symmetric operator sequence and of EcoRI endonuclease with its specific recognition site both exhibit a characteristic dependence of equilibrium binding constant (Kobs) on temperature, in which Kobs attains a relative maximum in the physiologically relevant...

journal_title：Journal of molecular biology

authors： Ha JH,Spolar RS,Record MT Jr

更新日期：1989-10-20 00:00:00

abstract：:Cholera toxin B-oligomer was imaged by atomic force microscopy (AFM) on biologically relevant model membranes, such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine and egg yolk phosphatidylcholine at room temperature in solution at a resolution in the range of 1 to 2 nm. In addition, two-dimensional arrays we...

journal_title：Journal of molecular biology

authors： Mou J,Yang J,Shao Z

更新日期：1995-05-05 00:00:00

abstract：:Histone deacetylases (HDACs) modify core histones and participate in large regulatory complexes that both suppress and enhance transcription. Recent studies indicate that some HDACs can act on non-histone proteins as well. Interest in these enzymes is growing because HDAC inhibitors appear to be promising therapeutic ...

journal_title：Journal of molecular biology

authors： Gregoretti IV,Lee YM,Goodson HV

更新日期：2004-04-16 00:00:00

abstract：:The Cip/Kip family of cyclin-dependent kinase (Cdk) inhibitors includes p21(Cip1), p27(Kip1) and p57(Kip2). Their kinase inhibitory activities are mediated by a homologous N-terminal kinase inhibitory domain. The Cdk inhibitory activity and stability of p27 have been shown to be regulated by a two-step phosphorylation...

journal_title：Journal of molecular biology

authors： Huang Y,Yoon MK,Otieno S,Lelli M,Kriwacki RW

更新日期：2015-01-30 00:00:00

abstract：:A set of 45 different tRNAs, each containing a single deoxynucleotide substitution covering the upper half of the molecule was used in conjunction with a high-throughput ribonuclease protection assay to investigate the thermodynamic role of 2' hydroxyl groups in stabilizing a complex with elongation factor Tu (EF-Tu) ...

journal_title：Journal of molecular biology

authors： Pleiss JA,Uhlenbeck OC

更新日期：2001-05-18 00:00:00

abstract：:We succeeded in expressing a chimeric myosin that comprises the motor domain of characean algal myosin, (the fastest known motor protein), and the neck and tail domains of Dictyostelium myosin II. Although the chimeric myosin showed an ATPase activity comparable to that of muscle myosin (15 times higher than that of t...

journal_title：Journal of molecular biology

authors： Kashiyama T,Ito K,Yamamoto K

更新日期：2001-08-17 00:00:00

abstract：:DBC2 is a tumor suppressor gene linked to breast and lung cancers. Although DBC2 belongs to the RHO GTPase family, it has a unique structure that contains a Broad-Complex/Tramtrack/Bric a Brac (BTB) domain at the C terminus instead of a typical CAAX motif. A limited number of functional studies on DBC2 have indicated ...

journal_title：Journal of molecular biology

authors： Chang FK,Sato N,Kobayashi-Simorowski N,Yoshihara T,Meth JL,Hamaguchi M

更新日期：2006-12-01 00:00:00

abstract：:Insertion sequence IS1 contains two reading frames, insA and B'-insB, which are responsible for its transposition, and was previously shown to express two proteins. The first, InsA, is the product of insA. The second, InsA-B'-InsB is a fusion of InsA with the product of B'-insB. Synthesis of this protein occurs by a -...

journal_title：Journal of molecular biology

authors： Matsutani S

更新日期：1994-07-01 00:00:00

abstract：:Nerve growth factor (NGF) is a member of the neurotrophin family. These growth factors support neuronal survival and differentiation. Neurotrophins are synthesized as pre-pro-proteins. Whereas the pre-sequences mediate secretion, the function of the pro-peptides is largely unknown. To test the role of the pro-sequence...

journal_title：Journal of molecular biology

authors： Rattenholl A,Ruoppolo M,Flagiello A,Monti M,Vinci F,Marino G,Lilie H,Schwarz E,Rudolph R

更新日期：2001-01-19 00:00:00

abstract：:We present a low resolution lattice model for which we can exhaustively generate all possible compact backbone conformations for small proteins. Using simple structural and energetic criteria, for a variety of proteins, we can select for lattice structures that have significant similarities with their known native str...

journal_title：Journal of molecular biology

authors： Hinds DA,Levitt M

更新日期：1994-11-04 00:00:00

abstract：:Six different hybrid colicins were constructed by recombining various domains of the two pore-forming colicins A and E1. These hybrid colicins were purified and their properties were studied. All of them were active against sensitive cells, although to varying degrees. From the results, one can conclude that: (1) the ...

journal_title：Journal of molecular biology

authors： Benedetti H,Frenette M,Baty D,Knibiehler M,Pattus F,Lazdunski C

更新日期：1991-02-05 00:00:00

abstract：:Forkhead box O4 (FOXO4) is a human transcription factor (TF) that participates in cell homeostasis. While the structure and DNA binding properties of the conserved forkhead domain (FHD) have been thoroughly investigated, how the transactivation domain (TAD) regulates the DNA binding properties of the protein remains e...

journal_title：Journal of molecular biology

authors： Kim J,Ahn D,Park CJ

更新日期：2021-01-13 00:00:00