Abstract:
:Bloom syndrome protein (BLM) is one of five human RecQ helicases that participate in DNA metabolism. RecQ C-terminal (RQC) domain is the main DNA binding module of BLM and specifically recognizes G-quadruplex (G4) DNA structures. Because G4 processing by BLM is essential for regulating replication and transcription, both G4 and BLM are considered as potential targets for anticancer therapy. Although several studies have revealed the detailed mechanism of G4 unwinding by BLM, the initial recognition of the G4 structure by the RQC domain is unclear. Here, we investigated the interaction between BLM RQC and the G4 DNA from the c-Myc promoter by NMR spectroscopy. While the signals broadened upon reciprocal titrations, the β-wing of RQC had significant chemical shift perturbations and experienced millisecond timescale dynamics upon G4 binding. A point mutation in the β-wing (N1164A) reduced G4 binding affinity. Our hydrogen-deuterium exchange data indicate that imino protons of G4 were exchanged with deuterium much faster in the presence of RQC. We suggest that RQC binds to G4 by using the β-wing as a separating pin to destabilize the G4. By providing information about the RQC-G4 interaction, our study yields insight into potential strategies for preventing G4 processing by BLM.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Lee S,Lee AR,Ryu KS,Lee JH,Park CJdoi
10.1016/j.jmb.2019.01.010subject
Has Abstractpub_date
2019-02-15 00:00:00pages
794-806issue
4eissn
0022-2836issn
1089-8638pii
S0022-2836(18)31136-7journal_volume
431pub_type
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