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Secondary structure constraints on the evolution of Drosophila 28 S ribosomal RNA expansion segments.

Abstract：

:Eukaryotic ribosomal RNA genes contain rapidly evolving regions of unknown function termed expansion segments. We present the comparative analysis of the primary and secondary structure of two expansion segments from the large subunit rRNA gene of ten species of Drosophila and the tsetse fly species Glossina morsitans morsitans. At the primary sequence level, most of the differences observed in the sequences obtained are single base substitutions. This is in marked contrast with observations in vertebrate species in which the insertion or deletion of repetitive motifs, probably generated by a DNA-slippage mechanism, is a major factor in the evolution of these regions. The secondary structure of the two regions, supported by multiple compensatory base changes, is highly conserved between the species examined and supports the existence of a general folding pattern for all eukaryotes. Intriguingly, the evolutionary rate of expansion segments is very slow relative to other genic and non-genic regions of the Drosophila genome. These results suggest that the evolution of expansion segments in the rDNA multigene family is a balance between the homogenization of new mutations by unequal crossing over and a combination of selection against some such mutations per se and selection for subsequent compensatory mutations, in order to maintain a particular RNA secondary structure.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Ruiz Linares A,Hancock JM,Dover GA

doi

10.1016/0022-2836(91)90178-9

subject

Has Abstract

pub_date

1991-06-05 00:00:00

pages

381-90

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(91)90178-9

journal_volume

219

pub_type

杂志文章

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