Determination of the MurD mechanism through crystallographic analysis of enzyme complexes.

abstract：:The structural and magnetic properties of the iron-cores of reconstituted horse spleen ferritin and Azotobacter vinelandii bacterioferritin have been investigated by high-resolution transmission electron microscopy, electron diffraction and Mossbauer spectroscopy. The structural properties of native horse spleen ferri...

journal_title：Journal of molecular biology

authors： Mann S,Williams JM,Treffry A,Harrison PM

更新日期：1987-12-05 00:00:00

abstract：:Prevailing evolutionary forces are typically deduced from the pattern of differences in synonymous and non-synonymous mutations, under the assumption of neutrality in the absence of amino acid change. We determined the complete sequence of ten vesicular stomatitis virus populations evolving under positive selection. A...

journal_title：Journal of molecular biology

authors： Novella IS,Zárate S,Metzgar D,Ebendick-Corpus BE

更新日期：2004-10-01 00:00:00

abstract：:Bovine IF(1) is a basic, 84 amino acid residue protein that inhibits the hydrolytic action of the F(1)F(0) ATP synthase in mitochondria under anaerobic conditions. Its oligomerization state is dependent on pH. At a pH value below 6.5 it forms an active dimer. At higher pH values, two dimers associate to form an inacti...

journal_title：Journal of molecular biology

authors： Gordon-Smith DJ,Carbajo RJ,Yang JC,Videler H,Runswick MJ,Walker JE,Neuhaus D

更新日期：2001-04-27 00:00:00

abstract：:The NtrC-family PhhR protein of Pseudomonas putida is involved in the control of the metabolism of aromatic amino acids, and it is a dual regulatory protein. When PhhR acts as an activator, it stimulates transcription from its cognate promoters with RNA polymerase/sigma(70) rather than with sigma(54), as is the case f...

journal_title：Journal of molecular biology

authors： Herrera MC,Krell T,Zhang X,Ramos JL

更新日期：2009-12-04 00:00:00

abstract：:Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) by favoring the activation of a water molecule coordinated to the active-site metal ion. Cys11, His185, Glu222 and Asp233 are the other metal ligands. Wild...

journal_title：Journal of molecular biology

authors： Wang J,Duewel HS,Stuckey JA,Woodard RW,Gatti DL

更新日期：2002-11-22 00:00:00

abstract：:We have applied a search strategy for determining the optimal packing of protein secondary structure elements to the rotational positioning of the seven transmembrane helices of bacteriorhodopsin. The search is based on the assumption that the relative orientations of the helices within the bundle are conditioned prin...

journal_title：Journal of molecular biology

authors： Tuffery P,Etchebest C,Popot JL,Lavery R

更新日期：1994-03-04 00:00:00

abstract：:6A6 is a murine monoclonal antibody raised against the humanized anti-tissue factor antibody D3H44. 6A6 is able to completely neutralize the anticoagulant activity of D3H44 in tissue factor-dependent functional assays, such as endotoxin-induced whole blood clotting, prothrombin time, as well as factor X and factor IX ...

journal_title：Journal of molecular biology

authors： Eigenbrot C,Meng YG,Krishnamurthy R,Lipari MT,Presta L,Devaux B,Wong T,Moran P,Bullens S,Kirchhofer D

更新日期：2003-08-08 00:00:00

abstract：:New insights into the modular organization and flexibility of the N-terminal half of human cardiac myosin binding protein C (cMyBP-C) and information on the association state of the full-length protein have been deduced from a combined small-angle X-ray scattering (SAXS) and NMR study. SAXS data show that the first fi...

journal_title：Journal of molecular biology

authors： Jeffries CM,Lu Y,Hynson RM,Taylor JE,Ballesteros M,Kwan AH,Trewhella J

更新日期：2011-12-16 00:00:00

abstract：:Non-structural protein 9 (Nsp9) of coronaviruses is believed to bind single-stranded RNA in the viral replication complex. The crystal structure of Nsp9 of human coronavirus (HCoV) 229E reveals a novel disulfide-linked homodimer, which is very different from the previously reported Nsp9 dimer of SARS coronavirus. In c...

journal_title：Journal of molecular biology

authors： Ponnusamy R,Moll R,Weimar T,Mesters JR,Hilgenfeld R

更新日期：2008-11-28 00:00:00

abstract：:Gap junction channels are intercellular channels that form by docking the extracellular loops of connexin protein subunits. While the structure and function of gap junctions as intercellular channels have been characterized using different techniques, the physics of the inter-connexin interaction remain unknown. Moreo...

journal_title：Journal of molecular biology

authors： Rico F,Oshima A,Hinterdorfer P,Fujiyoshi Y,Scheuring S

更新日期：2011-09-09 00:00:00

abstract：:In a few, rather rare cases, frameshift mutant alleles are phenotypically suppressed during limitation for particular aminoacyl-tRNA species. The simplest interpretation is compensatory ribosome frameshifting at a "hungry" codon in the vicinity of the suppressed frameshift mutation. We have now tested this interpretat...

journal_title：Journal of molecular biology

authors： Weiss R,Lindsley D,Falahee B,Gallant J

更新日期：1988-09-20 00:00:00

abstract：:The A-isozyme of O-acetylserine sulfhydrylase, a pyridoxal phosphate-dependent enzyme isolated from Salmonella typhimurium catalyzes the synthesis of L-cysteine from O-acetyl-L-serine and sulfide. The pyridoxal form of the enzyme has been crystallized in two different forms. One form is in the orthorhombic space group...

journal_title：Journal of molecular biology

authors： Rao GS,Mottonen J,Goldsmith EJ,Cook PF

更新日期：1993-06-20 00:00:00

abstract：:Human transcriptional coactivator PC4 is a highly abundant nuclear protein that is involved in diverse cellular processes ranging from transcription to chromatin organization. Earlier, we have shown that PC4, a positive activator of p53, overexpresses upon genotoxic insult in a p53-dependent manner. In the present stu...

journal_title：Journal of molecular biology

authors： Batta K,Yokokawa M,Takeyasu K,Kundu TK

更新日期：2009-01-23 00:00:00

abstract：:Much data support the concept that the MHC class I-related receptor FcRn serves to regulate immunoglobulin G (IgG) concentrations in serum and other diverse body sites in both rodents and humans. Previous studies have indicated that the human ortholog of FcRn is endowed with unexpectedly high stringency in binding spe...

journal_title：Journal of molecular biology

authors： Zhou J,Johnson JE,Ghetie V,Ober RJ,Ward ES

更新日期：2003-09-26 00:00:00

abstract：:The yeast a1 and alpha 2 proteins are examples of homeodomain proteins that display cell-type-specific expression. They are co-expressed in only one type of cell, the a/alpha cell, where they repress the expression of a group of target genes. Using purified proteins, we demonstrate that a1 and alpha 2 form a highly sp...

journal_title：Journal of molecular biology

authors： Goutte C,Johnson AD

更新日期：1993-10-05 00:00:00

abstract：:Current data suggest that globular domains may form amyloids via different mechanisms. Nevertheless, there are indications that the initiation of the process takes place invariably in the less stable segments of a protein domain. We have studied the sequence and structural conservation of beta(2)-microglobulin that de...

journal_title：Journal of molecular biology

authors： Benyamini H,Gunasekaran K,Wolfson H,Nussinov R

更新日期：2003-06-27 00:00:00

abstract：:Transcription of the rabbit alpha-globin gene begins primarily at the cap site, although some upstream start sites are also observed. Analysis by RNA polymerase run-on assays in nuclei shows that transcription continues at a high level past the polyadenylation site, after which the polymerase density actually increase...

journal_title：Journal of molecular biology

authors： Vandenbergh DJ,James-Pederson M,Hardison RC

更新日期：1991-07-20 00:00:00

abstract：:Na+/H+antiportersare a category of ubiquitous transmembrane proteins with various important physiological roles in almost all living organisms ranging from bacteria to humans. However, the knowledge of novel Na+/H+antiporters remains to be broadened, and the functional roles ofoligomerization in theseantiportershave n...

journal_title：Journal of molecular biology

authors： Shao L,Xu T,Zheng X,Shao D,Zhang H,Chen H,Zhang Z,Yan M,Abdel-Motaal H,Jiang J

更新日期：2021-01-22 00:00:00

abstract：:The p127 tumor suppressor protein encoded by the lethal(2)giant larvae, l(2)gl, gene of Drosophila melanogaster forms high molecular mass complexes consisting predominantly of p127 molecules. To determine whether p127 can self-assemble in the absence of other binding factors, we analyzed the size of in vitro synthesiz...

journal_title：Journal of molecular biology

authors： Jakobs R,de Lorenzo C,Spiess E,Strand D,Mechler BM

更新日期：1996-12-06 00:00:00

abstract：:The role of an 18-residue ion-pair network, that is present in the glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus, in conferring stability to other, less stable homologous enzymes, has been studied by introducing four new charged amino acid residues into the subunit interface of glutam...

journal_title：Journal of molecular biology

authors： Lebbink JH,Knapp S,van der Oost J,Rice D,Ladenstein R,de Vos WM

更新日期：1999-06-04 00:00:00

abstract：:A number of approaches have been utilized to generate antibodies to cancer cell surface receptors that can be used as potential therapeutics. A number of these therapeutic approaches, including antibody-drug conjugates, immunotoxins, and targeted nucleic acid delivery, require antibodies that not only bind receptor bu...

journal_title：Journal of molecular biology

authors： Zhou Y,Zou H,Zhang S,Marks JD

更新日期：2010-11-19 00:00:00

abstract：:It is generally believed that DNA replication in most eukaryotes proceeds according to a precise program in which there is a defined temporal order by which each chromosomal region is duplicated. However, the regularity of this program at the level of individual chromosomes, in terms of both the relative timing and th...

journal_title：Journal of molecular biology

authors： Czajkowsky DM,Liu J,Hamlin JL,Shao Z

更新日期：2008-01-04 00:00:00

abstract：:Single crystals of the soluble glucose dehydrogenase (GDH) from Acinetobacter calcoaceticus have been grown by the vapour diffusion method. These crystals diffract to beyond 2.1 A and are suitable for X-ray crystallography. The space group was determined to be P2(1) with unit cell parameters a = 55.5 A, b = 104.5 A, c...

journal_title：Journal of molecular biology

authors： Schlunegger MP,Grütter MG,Streiff MB,Olsthoorn AJ,Duine JA

更新日期：1993-10-20 00:00:00

abstract：:The papillomavirus E2 transcription and replication factors bind to the DNA consensus ACCGN(4)CGGT sequence (E2-BS), through both direct and indirect readout mechanisms. The two symmetric half-sites ACCG.CGGT are highly conserved in the genomes and are hydrogen bound with E2. Although E2 does not contact the N4 spacer...

journal_title：Journal of molecular biology

authors： Djuranovic D,Oguey C,Hartmann B

更新日期：2004-06-11 00:00:00

abstract：:Enterococcus faecalis, a ubiquitous member of the healthy human gut microbiota, is also a common opportunistic pathogen and leading cause of nosocomial infections. This tenacious microbe is well adapted to infect and persist in multiple niches within the mammalian host and can rapidly tune its metabolism to respond to...

journal_title：Journal of molecular biology

authors： Kao PHN,Kline KA

更新日期：2019-07-26 00:00:00

abstract：:Calmodulin (CaM) is an important regulator of Kv7.x (KCNQx) voltage-gated potassium channels. Channels from this family produce neuronal M currents and cardiac and auditory I(KS) currents and harbor mutations that cause arrhythmias, epilepsy, and deafness. Despite extensive functional characterization, biochemical and...

journal_title：Journal of molecular biology

authors： Xu Q,Chang A,Tolia A,Minor DL Jr

更新日期：2013-01-23 00:00:00

abstract：:The human signal recognition particle (SRP) is a large RNA-protein complex that targets secretory and membrane proteins to the endoplasmic reticulum membrane. The S domain of SRP is composed of roughly half of the 7SL RNA and four proteins (SRP19, SRP54, and the SRP68/72 heterodimer). In order to understand how the bi...

journal_title：Journal of molecular biology

authors： Menichelli E,Isel C,Oubridge C,Nagai K

更新日期：2007-03-16 00:00:00

abstract：:The mercury resistance locus encoded by Tn21 on the monocopy IncFII plasmid R100 (merTn21) consists of a metal-responsive activator/repressor, merR, which controls initiation of a polycistronic message that includes genes for the uptake (merTPC) and reduction (merA) of Hg2+ and merD, which may also play a minor regula...

journal_title：Journal of molecular biology

authors： Gambill BD,Summers AO

更新日期：1992-05-20 00:00:00

abstract：:Cholera toxin (CT) moves from the cell surface to the endoplasmic reticulum (ER) by retrograde vesicular transport. The catalytic subunit of CT (CTA1) then crosses the ER membrane and enters the cytosol in a process that involves the quality control mechanism of ER-associated degradation. The molecular details of this...

journal_title：Journal of molecular biology

authors： Massey S,Banerjee T,Pande AH,Taylor M,Tatulian SA,Teter K

更新日期：2009-11-13 00:00:00

abstract：:3-Methylitaconate-Delta-isomerase (Mii) participates in the nicotinate fermentation pathway of the anaerobic soil bacterium Eubacterium barkeri (order Clostridiales) by catalyzing the reversible conversion of (R)-3-methylitaconate (2-methylene-3-methylsuccinate) to 2,3-dimethylmaleate. The enzyme is also able to catal...

journal_title：Journal of molecular biology

authors： Velarde M,Macieira S,Hilberg M,Bröker G,Tu SM,Golding BT,Pierik AJ,Buckel W,Messerschmidt A

更新日期：2009-08-21 00:00:00