Abstract：
:Na+/H+antiportersare a category of ubiquitous transmembrane proteins with various important physiological roles in almost all living organisms ranging from bacteria to humans. However, the knowledge of novel Na+/H+antiporters remains to be broadened, and the functional roles ofoligomerization in theseantiportershave not yet been thoroughly understood. Here, we reported functional analysis of an unknown transmembrane protein composed of 103 amino acid residues. This protein was found to function as a Na+(Li+, K+)/H+ antiporter. To the best of our knowledge, this antiporter is the minimal one of known Na+/H+antiporters and thus designated as NhaM to represent the minimal Na+/H+antiporter. NhaM and its homologs have not yet been classified into any protein family. Based on phylogenetic analysis and protein alignment, we propose NhaM and its homologs to constitute a novel transporter family designated as NhaM family. More importantly, we found that NhaM is assembled with parallel protomers into a homo-oligomer and oligomerization is vital for the function of this antiporter. This implies that NhaM may adopt and require an oligomer structure for its normal function to create a similar X-shaped structure to that of the NhaA fold. Taken together, current findings not only present the proposal of a novel transporter family but also positively contribute to the functional roles of oligomerization in Na+/H+antiporters.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Shao L,Xu T,Zheng X,Shao D,Zhang H,Chen H,Zhang Z,Yan M,Abdel-Motaal H,Jiang Jdoi
10.1016/j.jmb.2020.166730subject
Has Abstractpub_date
2021-01-22 00:00:00pages
166730issue
2eissn
0022-2836issn
1089-8638pii
S0022-2836(20)30654-9journal_volume
433pub_type
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