当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Substitution of Asp114 or Arg116 in the fingers domain of moloney murine leukemia virus reverse transcriptase affects interactions with the template-primer resulting in decreased processivity.

Substitution of Asp114 or Arg116 in the fingers domain of moloney murine leukemia virus reverse transcriptase affects interactions with the template-primer resulting in decreased processivity.

Abstract：

:Reverse transcriptase, an essential retroviral DNA polymerase, replicates the single-stranded RNA genome of the retrovirus, producing a double-stranded DNA copy, which is subsequently integrated into the host's genome. Substitution of Ala for either Asp114 or Arg116, two highly conserved residues in the fingers domain of Moloney murine leukemia virus reverse transcriptase, results in enzymes (D114A or R116A) with significant defects in their abilities to processively synthesize DNA using RNA or DNA as a template. D114A and R116A enzymes also bind more weakly to template-primer in the presence of added deoxyribonucleotides, as seen by gel-shift analysis, but retain the ability to strand transfer and accumulate smaller RNase H cleavage products when compared to the wild-type enzyme. In addition, mutant proviruses, including D114A and R116A substitutions in Moloney murine leukemia virus reverse transcriptase, are not viable despite the presence of processed reverse transcriptase in the viral particles. A potential mechanistic role in processive synthesis for D114 and R116 is discussed in the context of our results, related studies on HIV-1 reverse transcriptase, and previous structural studies.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Gu J,Villanueva RA,Snyder CS,Roth MJ,Georgiadis MM

doi

10.1006/jmbi.2000.4281

keywords:

subject

Has Abstract

pub_date

2001-01-12 00:00:00

pages

341-59

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(00)94281-5

journal_volume

305

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Bacteriophage SPP1 pac Cleavage: A Precise Cut without Sequence Specificity Requirement.

    abstract：:In many tailed bacteriophages, DNA packaging is initiated by recognition and cleavage of a specific sequence pac by the small (TerS) and large (TerL) terminase subunits. It was previously shown that the SPP1 pac region has two sequences where TerS binds (pacR and pacL), flanking the segment where TerL cleaves the SPP1...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2017.01.005

    authors： Djacem K,Tavares P,Oliveira L

    更新日期：2017-05-05 00:00:00

  • Molecular structure of fd (f1, M13) filamentous bacteriophage refined with respect to X-ray fibre diffraction and solid-state NMR data supports specific models of phage assembly at the bacterial membrane.

    abstract：:Filamentous bacteriophage (Inovirus) is a simple and well-characterized model system. The phage particle, or virion, is about 60 angstroms in diameter and several thousand angstrom units long. The virions are assembled at the bacterial membrane as they extrude out of the host without killing it, an example of specific...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.10.048

    authors： Marvin DA,Welsh LC,Symmons MF,Scott WR,Straus SK

    更新日期：2006-01-13 00:00:00

  • Regulation of Mitochondrial Electron Transport Chain Assembly.

    abstract：:Mitochondrial function depends on the correct synthesis, transport, and assembly of proteins and cofactors of the electron transport chain. The initial idea that the respiratory chain protein complexes (RCCs) were independent structures in the inner mitochondrial membrane evolved after the identification of higher qua...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2018.09.016

    authors： Cogliati S,Lorenzi I,Rigoni G,Caicci F,Soriano ME

    更新日期：2018-12-07 00:00:00

  • Crystallization and subunit structure of canine myeloperoxidase.

    abstract：:Three crystal forms of canine myeloperoxidase are described. An orthorhombic form in space group P2(1)2(1)2(1) has unit cell dimensions: a = 108.3 A (1 A = 0.1 nm) b = 205.9 A and c = 139.9 A. A trigonal form in space group P3(1)21 or P3(2)21 has unit cell dimensions: a = b = 138.9 A and c = 145.2 A. A monoclinic form...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90413-x

    authors： Fenna RE

    更新日期：1987-08-20 00:00:00

  • In-vivo degradation pathway of an excised intervening sequence.

    abstract：:A study of the distribution in heterogeneous nuclear RNP (hnRNP) of the different forms of the first intervening sequence (IVSI) from the E3 transcription unit of adenovirus serotype 2 suggests a three-step pathway for IVS excision and degradation. First, the lariat IVS is formed in a large RNP of the size of premesse...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90480-3

    authors： Sittler A,Gallinaro H,Kister L,Jacob M

    更新日期：1987-10-20 00:00:00

  • Effect of flap mutations on structure of HIV-1 protease and inhibition by saquinavir and darunavir.

    abstract：:HIV-1 (human immunodeficiency virus type 1) protease (PR) and its mutants are important antiviral drug targets. The PR flap region is critical for binding substrates or inhibitors and catalytic activity. Hence, mutations of flap residues frequently contribute to reduced susceptibility to PR inhibitors in drug-resistan...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.05.062

    authors： Liu F,Kovalevsky AY,Tie Y,Ghosh AK,Harrison RW,Weber IT

    更新日期：2008-08-01 00:00:00

  • Folding studies on a knotted protein.

    abstract：:YibK is a 160 residue homodimeric protein belonging to the SPOUT class of methyltransferases. Proteins in this group all display a unique topological feature; the backbone polypeptide chain folds to form a deep trefoil knot. Such knotted structures were completely unpredicted, it being thought impossible for a protein...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.12.055

    authors： Mallam AL,Jackson SE

    更新日期：2005-03-11 00:00:00

  • High-resolution structural analysis of mammalian profilin 2a complex formation with two physiological ligands: the formin homology 1 domain of mDia1 and the proline-rich domain of VASP.

    abstract：:Profilins are small proteins capable of binding actin, poly-l-proline and other proline-rich sequences, and phosphatidylinositol (4,5)-bisphosphate. A number of proline-rich ligands for profilin have been characterised, including proteins of the Ena/VASP and formin families. We have determined the high-resolution crys...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.10.050

    authors： Kursula P,Kursula I,Massimi M,Song YH,Downer J,Stanley WA,Witke W,Wilmanns M

    更新日期：2008-01-04 00:00:00

  • Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli.

    abstract：:A specific complex of 5 S rRNA and several ribosomal proteins is an integral part of ribosomes in all living organisms. Here we studied the importance of Escherichia coli genes rplE, rplR and rplY, encoding 5 S rRNA-binding ribosomal proteins L5, L18 and L25, respectively, for cell growth, viability and translation. U...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.11.097

    authors： Korepanov AP,Gongadze GM,Garber MB,Court DL,Bubunenko MG

    更新日期：2007-03-02 00:00:00

  • In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities.

    abstract：:Human pseudouridine (Ψ) synthase Pus1 (hPus1) modifies specific uridine residues in several non-coding RNAs: tRNA, U2 spliceosomal RNA, and steroid receptor activator RNA. We report three structures of the catalytic core domain of hPus1 from two crystal forms, at 1.8Å resolution. The structures are the first of a mamm...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2013.05.014

    authors： Czudnochowski N,Wang AL,Finer-Moore J,Stroud RM

    更新日期：2013-10-23 00:00:00

  • Identifying determinants of recombination specificity: construction and characterization of mutant bacteriophage integrases.

    abstract：:The Integrases of bacteriophages lambda and HK022 promote recombination between DNA molecules that carry attachment sites. The two integrases are about 70% identical in sequence and catalyze nearly identical reactions, but recognize different sets of sites. To identify the amino acids that determine this difference in...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0486

    authors： Dorgai L,Yagil E,Weisberg RA

    更新日期：1995-09-15 00:00:00

  • The effect of a hydrophobic N-terminal probe on translational pausing of chloramphenicol acetyl transferase and rhodanese.

    abstract：:The effect on translational pausing of a hydrophobic probe, coumarin, at the N terminus of nascent peptides was investigated. Two different proteins, bacterial chloramphenicol acetyltransferase and bovine rhodanese, were synthesized by coupled transcription/translation in a cell-free system derived from Escherichia co...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2481

    authors： Tsalkova T,Kramer G,Hardesty B

    更新日期：1999-02-12 00:00:00

  • A ligand-centric analysis of the diversity and evolution of protein-ligand relationships in E.coli.

    abstract：:As enzymes evolve and diverge from common ancestor sequences, they often keep their overall reaction chemistry but specialize in the binding of different cognate ligands. This study borrows methods for the computational assessment of 2D similarity of small molecules from the field of chemoinformatics, to examine the e...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.01.061

    authors： Nobeli I,Spriggs RV,George RA,Thornton JM

    更新日期：2005-03-25 00:00:00

  • Structural evidence for effectiveness of darunavir and two related antiviral inhibitors against HIV-2 protease.

    abstract：:No drug has been targeted specifically for HIV-2 (human immunodeficiency virus type 2) infection despite its increasing prevalence worldwide. The antiviral HIV-1 (human immunodeficiency virus type 1) protease (PR) inhibitor darunavir and the chemically related GRL98065 and GRL06579A were designed with the same chemica...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.09.031

    authors： Kovalevsky AY,Louis JM,Aniana A,Ghosh AK,Weber IT

    更新日期：2008-12-05 00:00:00

  • Two secretory protein genes in Chironomus tentans have arisen by gene duplication and exhibit different developmental expression patterns.

    abstract：:The salivary gland cells in the dipteran Chironomus tentans produce approximately 15 different secretory proteins, with relative molecular masses ranging between 1 x 10(4) and 1 x 10(6). Together these proteins form two types of extra corporal tubes, a larval protective housing and feeding tube or a pupation tube. The...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1285

    authors： Galli J,Wieslander L

    更新日期：1993-05-20 00:00:00

  • Solvent rearrangement in an antigen-antibody interface introduced by site-directed mutagenesis of the antibody combining site.

    abstract：:The three-dimensional structure of a site-directed mutant of the bacterially expressed Fv fragment from monoclonal antibody D1.3, complexed to the specific antigen lysozyme has been determined to a nominal resolution of 1.8 A using X-ray diffraction data. The replacement of VL Trp92 by Asp allows two water molecules t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1309

    authors： Ysern X,Fields BA,Bhat TN,Goldbaum FA,Dall'Acqua W,Schwarz FP,Poljak RJ,Mariuzza RA

    更新日期：1994-05-13 00:00:00

  • Context-dependent remodeling of structure in two large protein fragments.

    abstract：:Protein folding involves the formation of secondary structural elements from the primary sequence and their association with tertiary assemblies. The relation of this primary sequence to a specific folded protein structure remains a central question in structural biology. An increasing body of evidence suggests that v...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.08.020

    authors： Schellenberg MJ,Ritchie DB,Wu T,Markin CJ,Spyracopoulos L,MacMillan AM

    更新日期：2010-10-01 00:00:00

  • Epitopes fused to F-pilin are incorporated into functional recombinant pili.

    abstract：:In order to develop a system which allows infection by an epitope-specific phage-antibody via an F-pilus expressing that epitope, a study on the expression of foreign sequences on F-pilin was undertaken. Initially, a plasmid library was constructed with random sequences encoding one to five amino acid residues fused t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1773

    authors： Rondot S,Anthony KG,Dübel S,Ida N,Wiemann S,Beyreuther K,Frost LS,Little M,Breitling F

    更新日期：1998-06-12 00:00:00

  • Synthesis and activity of piperazine-containing antirhinoviral agents and crystal structure of SDZ 880-061 bound to human rhinovirus 14.

    abstract：:A series of antipicornaviral agents containing piperazinyl moieties was synthesized with the objective of obtaining a compound with a broad spectrum of antirhinovirus activity, high potency (< or = 0.003 microgram/ml), and low cytotoxicity (> or = 30 micrograms/ml). Five compounds of this series were evaluated in deta...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0307

    authors： Oren DA,Zhang A,Nesvadba H,Rosenwirth B,Arnold E

    更新日期：1996-05-31 00:00:00

  • Effect of inorganic phosphate on FMN binding and loop flexibility in Desulfovibrio desulfuricans apo-flavodoxin.

    abstract：:The complex between flavin mononucleotide (FMN) and apo-flavodoxin is dominated by isoalloxazine-stacking interactions and 5'-phosphate hydrogen bonds. We show here that FMN binding to Desulfovibrio desulfuricans apo-flavodoxin is faster and the affinity is higher in the presence of inorganic phosphate as compared to ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.03.054

    authors： Muralidhara BK,Chen M,Ma J,Wittung-Stafshede P

    更新日期：2005-05-27 00:00:00

  • Electrostatic properties of two porin channels from Escherichia coli.

    abstract：:The electrostatic interactions in the channels of OmpF and PhoE porins from Escherichia coli were analysed on the basis of a macroscopic multi-dielectric model of the protein-membrane complex derived from the respective porin X-ray structures. The membrane was represented as layers of distinct dielectric constants cor...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1451

    authors： Karshikoff A,Spassov V,Cowan SW,Ladenstein R,Schirmer T

    更新日期：1994-07-22 00:00:00

  • Molecular evolution of the HBII-52 snoRNA cluster.

    abstract：:HBII-52 is a human brain-specific C/D box snoRNA that potentially regulates the editing and/or alternative splicing of the serotonin receptor. Forty-two nearly identical copies of the HBII-52 gene are located immediately downstream of the SNRPN protein-coding gene in an imprinted locus associated with Prader-Willi syn...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.06.057

    authors： Nahkuri S,Taft RJ,Korbie DJ,Mattick JS

    更新日期：2008-09-12 00:00:00

  • Polypyrimidine tract-binding protein inhibits translation of bip mRNA.

    abstract：:Translation initiation of human Bip mRNA is directed by an internal ribosomal entry site (IRES) located in the 5' non-translated region. No trans-acting factor possibly involved in this process has as of yet been identified. For the encephalomyocarditis virus and other picornaviruses, polypyrimidine tract-binding prot...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4179

    authors： Kim YK,Hahm B,Jang SK

    更新日期：2000-11-24 00:00:00

  • Fluid and condensed ApoA-I/phospholipid monolayers provide insights into ApoA-I membrane insertion.

    abstract：:Apolipoprotein A-I (ApoA-I) is a protein implicated in the solubilization of lipids and cholesterol from cellular membranes. The study of ApoA-I in phospholipid (PL) monolayers brings relevant information about ApoA-I/PL interactions. We investigated the influence of PL charge and acyl chain organization on the intera...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2011.04.006

    authors： Chièze L,Bolanos-Garcia VM,Pinot M,Desbat B,Renault A,Beaufils S,Vié V

    更新日期：2011-07-01 00:00:00

  • Guanidinobenzoic acid inhibitors of influenza virus neuraminidase.

    abstract：:The active site of influenza virus neuraminidase (NA) is formed by 11 universally conserved residues. A guanidino group incorporated into two unrelated NA inhibitors was previously reported to occupy different negatively charged sites in the NA active site, A new inhibitor containing two guanidino groups was synthesiz...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0885

    authors： Sudbeck EA,Jedrzejas MJ,Singh S,Brouillette WJ,Air GM,Laver WG,Babu YS,Bantia S,Chand P,Chu N,Montgomery JA,Walsh DA,Luo M

    更新日期：1997-04-04 00:00:00

  • Local encoding of computationally designed enzyme activity.

    abstract：:One aim of computational protein design is to introduce novel enzyme activity into proteins of known structure by predicting mutations that stabilize transition states. Previously, we showed that it is possible to introduce triose phosphate isomerase activity into the ribose-binding protein of Escherichia coli by cons...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.12.002

    authors： Allert M,Dwyer MA,Hellinga HW

    更新日期：2007-02-23 00:00:00

  • Recent advances in the molecular biology of hepatitis C virus.

    abstract：:The Hepatitis C virus is a positive-stranded RNA virus which is the causal agent for a chronic liver infection afflicting more than 170,000,000 people world-wide. The HCV genome is approximately 9.6 kb in length and the proteome encoded is a polyprotein of a little more than 3000 amino acid residues. This polyprotein ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1006/jmbi.2001.5055

    authors： Rosenberg S

    更新日期：2001-10-26 00:00:00

  • Threshold occupancy and specific cation binding modes in the hammerhead ribozyme active site are required for active conformation.

    abstract：:The relationship between formation of active in-line attack conformations and monovalent (Na(+)) and divalent (Mg(2+)) metal ion binding in hammerhead ribozyme (HHR) has been explored with molecular dynamics simulations. To stabilize repulsions between negatively charged groups, different requirements of the threshold...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.02.054

    authors： Lee TS,Giambaşu GM,Sosa CP,Martick M,Scott WG,York DM

    更新日期：2009-04-24 00:00:00

  • DBC2 is essential for transporting vesicular stomatitis virus glycoprotein.

    abstract：:DBC2 is a tumor suppressor gene linked to breast and lung cancers. Although DBC2 belongs to the RHO GTPase family, it has a unique structure that contains a Broad-Complex/Tramtrack/Bric a Brac (BTB) domain at the C terminus instead of a typical CAAX motif. A limited number of functional studies on DBC2 have indicated ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.09.026

    authors： Chang FK,Sato N,Kobayashi-Simorowski N,Yoshihara T,Meth JL,Hamaguchi M

    更新日期：2006-12-01 00:00:00

  • Effects of rifampicin resistant rpoB mutations on antitermination and interaction with nusA in Escherichia coli.

    abstract：:Rifampicin resistant (Rifr mutations map in the rpoB gene encoding the beta subunit of Escherichia coli RNA polymerase. We have used our collection of 17 sequenced Rifr mutations to investigate the involvement of E. coli RNA polymerase in the antitermination systems enhancing expression of delayed early lambda genes o...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90573-6

    authors： Jin DJ,Cashel M,Friedman DI,Nakamura Y,Walter WA,Gross CA

    更新日期：1988-11-20 00:00:00