Regulation of Mitochondrial Electron Transport Chain Assembly.

abstract：:DNA methyltransferases flip their target base out of the DNA helix. Here, we have investigated base flipping by wild-type EcoRV DNA methyltransferase (M.EcoRV) and five M.EcoRV variants (D193A, Y196A, S229A, W231R and Y258A). These variants bind to DNA and S-adenosylmethionine but have a severely reduced catalytic eff...

journal_title：Journal of molecular biology

authors： Jeltsch A,Roth M,Friedrich T

更新日期：1999-01-22 00:00:00

abstract：:The rate of ligation of DNA molecules into circular forms depends on the ring closure probability, commonly called the j-factor, which is a sensitive measure of the extent to which thermal fluctuations contribute to bending and twisting of DNA molecules in solution. We present a theoretical treatment of the cyclizatio...

journal_title：Journal of molecular biology

authors： Levene SD,Crothers DM

更新日期：1986-05-05 00:00:00

abstract：:Mammalian Cdc25 phosphatase is responsible for the dephosphorylation of Cdc2 and other cyclin-dependent kinases at Thr14 and Tyr15, thus activating the kinase and allowing cell cycle progression. The catalytic domain of this dual-specificity phosphatase has recently been mapped to the 180 most C-terminal amino acids. ...

journal_title：Journal of molecular biology

authors： Hofmann K,Bucher P,Kajava AV

更新日期：1998-09-11 00:00:00

abstract：:We have isolated and sequenced a cloned complementary DNA insert complementary to the messenger RNA of a cytoplasmic actin expressed in human epidermal cells. This provides the first cytoplasmic actin complementary DNA sequence for a vertebrate organism. The actin amino acid sequence predicted from this complementary ...

journal_title：Journal of molecular biology

authors： Hanukoglu I,Tanese N,Fuchs E

更新日期：1983-02-05 00:00:00

abstract：:Scaffold or matrix-attachment regions (S/MARs) are thought to be involved in the organization of eukaryotic chromosomes and in the regulation of several DNA functions. Their characteristics are conserved between plants and humans, and a variety of biological activities have been associated with them. The identificatio...

journal_title：Journal of molecular biology

authors： Bode J,Winkelmann S,Götze S,Spiker S,Tsutsui K,Bi C,A K P,Benham C

更新日期：2006-04-28 00:00:00

abstract：:The transcriptional co-activators CBP/p300 and PCAF participate in transcriptional activation by many factors. We have shown that both CBP/p300 and PCAF stimulate the transcriptional activation by KLF13, a member of the KLF/Sp1 family, either individually or cooperatively. Here we further investigated how CBP and PCAF...

journal_title：Journal of molecular biology

authors： Song CZ,Keller K,Chen Y,Stamatoyannopoulos G

更新日期：2003-05-30 00:00:00

abstract：:The bacterial flagellar motor is a tiny molecular machine that uses a transmembrane flux of H(+) or Na(+) ions to drive flagellar rotation. In proton-driven motors, the membrane proteins MotA and MotB interact via their transmembrane regions to form a proton channel. The sodium-driven motors that power the polar flage...

journal_title：Journal of molecular biology

authors： Asai Y,Yakushi T,Kawagishi I,Homma M

更新日期：2003-03-21 00:00:00

abstract：:The HIV gp41 protein catalyzes fusion between viral and target cell membranes. Although the ~20-residue N-terminal fusion peptide (FP) region is critical for fusion, the structure of this region is not well characterized in large gp41 constructs that model the gp41 state at different times during fusion. This paper de...

journal_title：Journal of molecular biology

authors： Sackett K,Nethercott MJ,Zheng Z,Weliky DP

更新日期：2014-03-06 00:00:00

abstract：:The phycobilisome light-harvesting antenna in cyanobacteria and red algae is assembled from two substructures: a central core composed of allophycocyanin surrounded by rods that always contain phycocyanin (PC). Unpigmented proteins called linkers are also found within the rods and core. We present here two new structu...

journal_title：Journal of molecular biology

authors： David L,Marx A,Adir N

更新日期：2011-01-07 00:00:00

abstract：:The integrase protein (Int) from bacteriophage lambda is the archetypal member of the tyrosine recombinase family, a large group of enzymes that rearrange DNA in all domains of life. Int catalyzes the insertion and excision of the viral genome into and out of the Escherichia coli chromosome. Recombination transpires w...

journal_title：Journal of molecular biology

authors： Fadeev EA,Sam MD,Clubb RT

更新日期：2009-05-15 00:00:00

abstract：:RepA protein of plasmid P1 binds to arrays of 19 bp repeat sequences (iterons) and mediates initiation of replication and its control. Escherichia coli heat shock proteins DnaJ and DnaK can stimulate iteron binding activity of RepA in an ATP-dependent fashion. It has been proposed that RepA binds to DNA as monomers an...

journal_title：Journal of molecular biology

authors： DasGupta S,Mukhopadhyay G,Papp PP,Lewis MS,Chattoraj DK

更新日期：1993-07-05 00:00:00

abstract：:The ClpP component Clp protease from Escherichia coli has been crystallized and examined by X-ray crystallography and self-rotation function calculations. The crystal belongs to the monoclinic space group P2(1) with unit cell dimensions of a = 196.9 A, b = 104.3 A, c = 162.4 A and beta = 98.3 degrees. The X-ray diffra...

journal_title：Journal of molecular biology

authors： Shin DH,Lee CS,Chung CH,Suh SW

更新日期：1996-09-20 00:00:00

abstract：:Kinetic models were developed to describe the influence of prolyl peptide bond isomerization on the kinetics of reversible protein folding for cases in which structural intermediates do not occur. In the simulations, the number of prolyl residues and the relative rates of folding and isomerization were varied. The exp...

journal_title：Journal of molecular biology

authors： Kiefhaber T,Kohler HH,Schmid FX

更新日期：1992-03-05 00:00:00

abstract：:Six different hybrid colicins were constructed by recombining various domains of the two pore-forming colicins A and E1. These hybrid colicins were purified and their properties were studied. All of them were active against sensitive cells, although to varying degrees. From the results, one can conclude that: (1) the ...

journal_title：Journal of molecular biology

authors： Benedetti H,Frenette M,Baty D,Knibiehler M,Pattus F,Lazdunski C

更新日期：1991-02-05 00:00:00

abstract：:The structure of the recombinant medium chain alcohol dehydrogenase (ADH) from the hyperthermophilic archaeon Aeropyrum pernix has been solved by the multiple anomalous dispersion technique using the signal from the naturally occurring zinc ions. The enzyme is a tetramer with 222 point group symmetry. The ADH monomer ...

journal_title：Journal of molecular biology

authors： Guy JE,Isupov MN,Littlechild JA

更新日期：2003-08-29 00:00:00

abstract：:RanBPM and RanBP10 are non-canonical members of the Ran binding protein family that lack the Ran binding domain and do not associate with Ran GTPase in vivo. Rather, they have been shown to be scaffolding proteins that are important for a variety of cellular processes, and both of these proteins contain a SPRY domain,...

journal_title：Journal of molecular biology

authors： Hong SK,Kim KH,Song EJ,Kim EE

更新日期：2016-10-23 00:00:00

abstract：:Long-standing questions on how peptides fold are addressed by the simulation at different temperatures of the reversible folding of a peptide in solution in atomic detail. Molecular dynamics simulations correctly predict the structure that is thermodynamically stable at 298 K, irrespective of the initial peptide confo...

journal_title：Journal of molecular biology

authors： Daura X,Jaun B,Seebach D,van Gunsteren WF,Mark AE

更新日期：1998-07-31 00:00:00

abstract：:Bloom syndrome protein forms an oligomeric ring structure and belongs to a group of DNA helicases showing extensive homology to the Escherichia coli DNA helicase RecQ, a suppressor of illegitimate recombination. After over-production in E.coli, we have purified the RecQ core of BLM consisting of the DEAH, RecQ-Ct and ...

journal_title：Journal of molecular biology

authors： Janscak P,Garcia PL,Hamburger F,Makuta Y,Shiraishi K,Imai Y,Ikeda H,Bickle TA

更新日期：2003-06-27 00:00:00

abstract：:The steps of UUC recognition by tRNAPhe were analysed by temperature-jump measurements. At ion concentrations close to physiological conditions we found three relaxation processes, which we assigned to (1) formation of codon-anticodon complexes, (2) a conformational change of the anticodon loop coupled with Mg2+ bindi...

journal_title：Journal of molecular biology

authors： Labuda D,Striker G,Porschke D

更新日期：1984-04-25 00:00:00

abstract：:We have used rapid probing methods to follow the changes in reactivity of residues in 16 S rRNA to chemical and enzymatic probes as ribosomal proteins S2, S3, S10, S13 and S14 are assembled into 30 S subunits. Effects observed are confined to the 3' major domain of the RNA and comprise three general classes. (1) Monos...

journal_title：Journal of molecular biology

authors： Powers T,Stern S,Changchien LM,Noller HF

更新日期：1988-06-20 00:00:00

abstract：:A tumor is a heterogeneous population of cells that provides an environment in which every cell resides in a microenvironmental niche. Microscopic evaluation of tissue sections, based on histology and immunohistochemistry, has been a cornerstone in pathology for decades. However, the dawn of novel technologies to inve...

journal_title：Journal of molecular biology

authors： Koos B,Kamali-Moghaddam M,David L,Sobrinho-Simões M,Dimberg A,Nilsson M,Wählby C,Söderberg O

更新日期：2015-06-05 00:00:00

abstract：:The crystal structures of glycosylated native proteinase A, an aspartic proteinase found in the vacuole of Saccharomyces cerevisiae, and its complex with a difluorostatone-containing tripeptide have been determined by molecular replacement to 3.5 A and 2.4 A resolutions, respectively. Superposition of the bound and na...

journal_title：Journal of molecular biology

authors： Aguilar CF,Cronin NB,Badasso M,Dreyer T,Newman MP,Cooper JB,Hoover DJ,Wood SP,Johnson MS,Blundell TL

更新日期：1997-04-11 00:00:00

abstract：:The ribonucleoprotein (RNP) domain is one of the most common eukaryotic protein domains, and is found in many proteins involved in recognition of a wide variety of RNAs. Two structures of RNA complexes of human U1A protein have revealed important aspects of RNP-RNA recognition, but have also raised intriguing question...

journal_title：Journal of molecular biology

authors： Mittermaier A,Varani L,Muhandiram DR,Kay LE,Varani G

更新日期：1999-12-10 00:00:00

abstract：:Here, we show that in the chicken genome, the domain of alpha-globin genes is preceded by a CpG island of which the downstream part ( approximately 0.65 kbp) is heavily methylated in lymphoid cells; it is either non-methylated or undermethylated in erythroid cells. Recombinant plasmids were constructed with the corres...

journal_title：Journal of molecular biology

authors： Razin SV,Ioudinkova ES,Scherrer K

更新日期：2000-06-16 00:00:00

abstract：:A homologue of the Escherichia coli GroEL apical domain was obtained from thermophilic eubacterium Thermus thermophilus. The domains share 70 % sequence identity (101 out of 145 residues). The thermal stability of the T. thermophilus apical domain (Tm>100 degrees C as evaluated by circular dichroism) is at least 35 de...

journal_title：Journal of molecular biology

authors： Hua Q,Dementieva IS,Walsh MA,Hallenga K,Weiss MA,Joachimiak A

更新日期：2001-02-23 00:00:00

abstract：:Amyloid deposits in the pancreatic islets of Langerhans are thought to be a main factor responsible for death of the insulin-producing islet beta-cells in type 2 diabetes. It is hypothesized that beta-cell death is related to interaction of the 37 amino acid residue human islet amyloid polypeptide (hIAPP), the major c...

journal_title：Journal of molecular biology

authors： Engel MF,Yigittop H,Elgersma RC,Rijkers DT,Liskamp RM,de Kruijff B,Höppener JW,Antoinette Killian J

更新日期：2006-02-24 00:00:00

abstract：:Proteinaceous serine proteinase inhibitors are widespread throughout the plant kingdom where they play an important role in protection against pests and pathogens. Here, we describe the isolation and characterisation of a novel 14 amino acid residue cyclic peptide from sunflower seeds, which is a potent inhibitor of t...

journal_title：Journal of molecular biology

authors： Luckett S,Garcia RS,Barker JJ,Konarev AV,Shewry PR,Clarke AR,Brady RL

更新日期：1999-07-09 00:00:00

abstract：:Mammalian alphaB-crystallin is highly expressed both in lens epithelium and lens fibers. In contrast, gammaF-crystallin is highly expressed in the lens fiber cells. Crystallin gene expression in lens is regulated at the level of transcription by a sparse number of specific DNA-binding transcription factors. Here, we r...

journal_title：Journal of molecular biology

authors： Yang Y,Chauhan BK,Cveklova K,Cvekl A

更新日期：2004-11-19 00:00:00

abstract：:The preferred DNase I cleavage sites within the 160 bp tyrT DNA fragment were identified by studying the initial rate of cleavage of individual bonds. The results show that there is no correlation between the rate of cleavage and the identity of the dinucleotide sequence that is cleaved. Examination of the sequences s...

journal_title：Journal of molecular biology

authors： Herrera JE,Chaires JB

更新日期：1994-02-18 00:00:00

abstract：:In actin from many species H73 is methylated, but the function of this rare post-translational modification is unknown. Although not within bonding distance, it is located close to the gamma-phosphate of the actin-bound ATP. In most crystal structures of actin, the delta1-nitrogen of the methylated H73 forms a hydroge...

journal_title：Journal of molecular biology

authors： Nyman T,Schüler H,Korenbaum E,Schutt CE,Karlsson R,Lindberg U

更新日期：2002-04-05 00:00:00