Context-dependent remodeling of structure in two large protein fragments.


:Protein folding involves the formation of secondary structural elements from the primary sequence and their association with tertiary assemblies. The relation of this primary sequence to a specific folded protein structure remains a central question in structural biology. An increasing body of evidence suggests that variations in homologous sequence ranging from point mutations to substantial insertions or deletions can yield stable proteins with markedly different folds. Here we report the structural characterization of domain IV (D4) and ΔD4 (polypeptides with 222 and 160 amino acids, respectively) that differ by virtue of an N-terminal deletion of 62 amino acids (28% of the overall D4 sequence). The high-resolution crystal structures of the monomeric D4 and the dimeric ΔD4 reveal substantially different folds despite an overall conservation of secondary structure. These structures show that the formation of tertiary structures, even in extended polypeptide sequences, can be highly context dependent, and they serve as a model for structural plasticity in protein isoforms.


J Mol Biol


Schellenberg MJ,Ritchie DB,Wu T,Markin CJ,Spyracopoulos L,MacMillan AM




Has Abstract


2010-10-01 00:00:00














  • Crystal structure of tryptophanase.

    abstract::The X-ray structure of tryptophanase (Tnase) reveals the interactions responsible for binding of the pyridoxal 5'-phosphate (PLP) and atomic details of the K+ binding site essential for catalysis. The structure of holo Tnase from Proteus vulgaris (space group P2(1)2(1)2(1) with a = 115.0 A, b = 118.2 A, c = 153.7 A) h...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Isupov MN,Antson AA,Dodson EJ,Dodson GG,Dementieva IS,Zakomirdina LN,Wilson KS,Dauter Z,Lebedev AA,Harutyunyan EH

    更新日期:1998-02-27 00:00:00

  • Effect of inorganic phosphate on FMN binding and loop flexibility in Desulfovibrio desulfuricans apo-flavodoxin.

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    journal_title:Journal of molecular biology

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    authors: Muralidhara BK,Chen M,Ma J,Wittung-Stafshede P

    更新日期:2005-05-27 00:00:00

  • Amyloidogenic propensities and conformational properties of ProIAPP and IAPP in the presence of lipid bilayer membranes.

    abstract::Human islet amyloid polypeptide (hIAPP), which is considered the primary culprit for beta-cell loss in type 2 diabetes mellitus patients, is synthesized in beta-cells of the pancreas from its precursor pro-islet amyloid polypeptide (proIAPP), which may be important in early intracellular amyloid formation as well. We ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Jha S,Sellin D,Seidel R,Winter R

    更新日期:2009-06-26 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章,评审


    authors: Kneale GG

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    journal_title:Journal of molecular biology

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    authors: Lipman DJ,Wilbur WJ

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Ghosh B,Grzadzielska E,Bhattacharya P,Peralta E,DeVito J,Das A

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    pub_type: 杂志文章


    authors: Maravić G,Feder M,Pongor S,Flögel M,Bujnicki JM

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Sanders J,Towner P

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  • X-ray structure of recombinant ricin A-chain at 1.8 A resolution.

    abstract::Ricin is a potent plant toxin which acts by removing a specific adenine residue from the ribosome. The X-ray crystal structure of a new, tetragonal crystal form of the recombinant ricin A-chain diffracting to 1.8 A resolution has been determined via molecular replacement methods and refined to a crystallographic R-fac...

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    pub_type: 杂志文章


    authors: Weston SA,Tucker AD,Thatcher DR,Derbyshire DJ,Pauptit RA

    更新日期:1994-12-09 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Pickett SD,Saqi MA,Sternberg MJ

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Liou GF,Yoshizawa S,Courvalin P,Galimand M

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    journal_title:Journal of molecular biology

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    authors: Turner B,Lilley DM

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Prince SM,Papiz MZ,Freer AA,McDermott G,Hawthornthwaite-Lawless AM,Cogdell RJ,Isaacs NW

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    pub_type: 杂志文章


    authors: Hagervall TG,Pomerantz SC,McCloskey JA

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    authors: Onesti S,Lloyd LF,Maeder D,Mistry A,Brick P

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    journal_title:Journal of molecular biology

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    authors: Xue X,Lehming N

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Jacobsen KD,Willumsen BM

    更新日期:1995-09-22 00:00:00

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    abstract::Translation initiation of human Bip mRNA is directed by an internal ribosomal entry site (IRES) located in the 5' non-translated region. No trans-acting factor possibly involved in this process has as of yet been identified. For the encephalomyocarditis virus and other picornaviruses, polypyrimidine tract-binding prot...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kim YK,Hahm B,Jang SK

    更新日期:2000-11-24 00:00:00

  • Molecular basis for mutation in a surface protein expressed by malaria parasites.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Hudson DE,Wellems TE,Miller LH

    更新日期:1988-10-05 00:00:00

  • Structural and functional analysis of a human 7 S K RNA gene.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Krüger W,Benecke BJ

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  • Sequence Specificity in the Entropy-Driven Binding of a Small Molecule and a Disordered Peptide.

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    journal_title:Journal of molecular biology

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    journal_title:Journal of molecular biology

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    更新日期:2004-01-23 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Villaverde A,Martínez-Salas E,Domingo E

    更新日期:1988-12-05 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Hills RD Jr,Brooks CL 3rd

    更新日期:2008-10-03 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Orth P,Schnappinger D,Sum PE,Ellestad GA,Hillen W,Saenger W,Hinrichs W

    更新日期:1999-01-15 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Wojcik J,Mornon JP,Chomilier J

    更新日期:1999-06-25 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 评论,杂志文章


    authors: Lee SY,Pullen L,Virgil DJ,Castañeda CA,Abeykoon D,Bolon DN,Fushman D

    更新日期:2014-04-03 00:00:00

  • Identification of 5-hydroxycytidine at position 2501 concludes characterization of modified nucleotides in E. coli 23S rRNA.

    abstract::Complete characterization of a biomolecule's chemical structure is crucial in the full understanding of the relations between their structure and function. The dominating components in ribosomes are ribosomal RNAs (rRNAs), and the entire rRNA-but a single modified nucleoside at position 2501 in 23S rRNA-has previously...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Havelund JF,Giessing AM,Hansen T,Rasmussen A,Scott LG,Kirpekar F

    更新日期:2011-08-19 00:00:00

  • The foldon substructure of staphylococcal nuclease.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Bédard S,Mayne LC,Peterson RW,Wand AJ,Englander SW

    更新日期:2008-02-29 00:00:00