Abstract:
:We report the solution of the crystal structure of a mutant of the immunoglobulin VL domain of the antibody McPC603, in which the complementarity-determining region 1 segment is replaced with that of a different antibody. The wild-type and mutant crystal structures have been refined to a crystallographic R-factor of 14.9% at a nominal resolution of 1.97 A. A detailed description of the structures is given. Crystal packing results in a dimeric association of domains, in a fashion closely resembling that of an Fv fragment. The comparison of this VL domain with the same domain in the Fab fragment of McPC603 shows that the structure of an immunoglobulin VL domain is largely independent of its mode of association, even in places where the inter-subunit contacts are not conserved between VL and VH. In all three complementarity-determining regions we observe conformations that would not have been predicted by the canonical structure hypothesis. Significant differences between the VL domain dimer and the Fab fragment in the third complementarity-determining region show that knowledge of the structure of the dimerization partner and its exact mode of association may be needed to predict the precise conformation of antigen-binding loops.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Steipe B,Plückthun A,Huber Rdoi
10.1016/0022-2836(92)90398-4keywords:
subject
Has Abstractpub_date
1992-06-05 00:00:00pages
739-53issue
3eissn
0022-2836issn
1089-8638pii
0022-2836(92)90398-4journal_volume
225pub_type
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