Different intrastrand and interstrand contributions to stacking account for roll variations at the alternating purine-pyrimidine sequences in A-DNA and A-RNA.

abstract：:Although multiple viruses utilize host cell cyclophilins, including severe acute respiratory syndrome (SARS) and human immunodeficiency virus type-1(HIV-1), their role in infection is poorly understood. To help elucidate these roles, we have characterized the first virally encoded cyclophilin (mimicyp) derived from th...

journal_title：Journal of molecular biology

authors： Thai V,Renesto P,Fowler CA,Brown DJ,Davis T,Gu W,Pollock DD,Kern D,Raoult D,Eisenmesser EZ

更新日期：2008-04-18 00:00:00

abstract：:A hallmark of the catalytically essential Walker B motif of P-loop NTPases is the presence of an acidic residue (aspartate/glutamate) for efficient Mg2+ coordination. Although the Walker B motif has been identified in well-studied examples of P-loop NTPases, its identity is ambiguous in many families, for example, in ...

journal_title：Journal of molecular biology

authors： Kanade M,Chakraborty S,Shelke SS,Gayathri P

更新日期：2020-09-18 00:00:00

abstract：:The molecular chaperone Hsp90 is essential for the correct folding, maturation and activation of a diverse array of client proteins, including several key constituents of oncogenic processes. Hsp90 has become a focus of cancer research, since it represents a target for direct prophylaxis against multistep malignancy. ...

journal_title：Journal of molecular biology

authors： Phillips JJ,Yao ZP,Zhang W,McLaughlin S,Laue ED,Robinson CV,Jackson SE

更新日期：2007-10-05 00:00:00

abstract：:We report the solution of the crystal structure of a mutant of the immunoglobulin VL domain of the antibody McPC603, in which the complementarity-determining region 1 segment is replaced with that of a different antibody. The wild-type and mutant crystal structures have been refined to a crystallographic R-factor of 1...

journal_title：Journal of molecular biology

authors： Steipe B,Plückthun A,Huber R

更新日期：1992-06-05 00:00:00

abstract：:An analysis was performed on 335 pairs of structurally aligned proteins derived from the structural classification of proteins (SCOP http://scop.mrc-lmb.cam.ac.uk/scop/) database. These similarities were divided into analogues, defined as proteins with similar three-dimensional structures (same SCOP fold classificatio...

journal_title：Journal of molecular biology

authors： Russell RB,Saqi MA,Sayle RA,Bates PA,Sternberg MJ

更新日期：1997-06-13 00:00:00

abstract：:Transmembrane proteins (TMPs) play important roles in many biological processes, such as cell recognition and communication. Their structures are crucial for revealing complex functions but are hard to obtain. A variety of computational algorithms have been proposed to fill the gap by predicting structures from primar...

journal_title：Journal of molecular biology

authors： Feng SH,Zhang WX,Yang J,Yang Y,Shen HB

更新日期：2020-02-14 00:00:00

abstract：:Viral RNA helicases of the NS3/NPH-II group unwind RNA duplexes by processive, directional translocation on one of the duplex strands. The translocation is preceded by a poorly understood unwinding initiation phase. For NPH-II from vaccinia virus, unwinding initiation is rate limiting for the overall unwinding reactio...

journal_title：Journal of molecular biology

authors： Fairman-Williams ME,Jankowsky E

更新日期：2012-02-03 00:00:00

abstract：:Replication of plasmid ColE1 is regulated by a plasmid-specified small RNA (RNA I). RNA I binds to the precursor (RNA II) of the primer for DNA synthesis and inhibits primer formation. The process of binding of RNA I to RNA II that results in formation of a stably bound complex consists of a series of reactions formin...

journal_title：Journal of molecular biology

authors： Tomizawa J

更新日期：1990-04-20 00:00:00

abstract：:The dielectric constants of sickle cell hemoglobin were determined before and after gelation. The dielectric properties of oxy and deoxy sickle cell hemoglobin in solution are nearly identical to those of oxy and deoxy hemoglobin A. Only in the gel state did deoxy sickle cell hemoglobin display dielectric behavior dif...

journal_title：Journal of molecular biology

authors： Delalic Z,Takashima S,Adachi K,Asakura T

更新日期：1983-08-15 00:00:00

abstract：:Glycosaminoglycans (GAGs) are related to multiple biological functions and diseases. There is growing evidence that GAG concentration and sulfate content increase with age. The destabilizing mutation A546T in the corneal protein TGFBIp leads to lattice-type corneal dystrophy, but symptoms only appear in the fourth dec...

journal_title：Journal of molecular biology

authors： Malmos KG,Stenvang M,Sahin C,Christiansen G,Otzen DE

更新日期：2017-09-01 00:00:00

abstract：:Using purified RNA from HeLa cells, we have synthesized and cloned a cDNA encoding an almost entire 7 S K RNA. This cDNA probe was used to isolate 7 S K RNA gene sequences from a human genomic library by high-stringency colony hybridization. In order to differentiate between functional genes and related sequences, we ...

journal_title：Journal of molecular biology

authors： Krüger W,Benecke BJ

更新日期：1987-05-05 00:00:00

abstract：:To investigate systematically the RNA sequences necessary for aminoacylation by Escherichia coli leucyl-tRNA synthetase, RNAs with leucylation activity were isolated by in vitro selection from a library of tRNALeu variants possessing randomized sequences in the D-loop, the variable arm, and the T-loop. After two round...

journal_title：Journal of molecular biology

authors： Asahara H,Nameki N,Hasegawa T

更新日期：1998-10-30 00:00:00

abstract：:The X-ray structure of tryptophanase (Tnase) reveals the interactions responsible for binding of the pyridoxal 5'-phosphate (PLP) and atomic details of the K+ binding site essential for catalysis. The structure of holo Tnase from Proteus vulgaris (space group P2(1)2(1)2(1) with a = 115.0 A, b = 118.2 A, c = 153.7 A) h...

journal_title：Journal of molecular biology

authors： Isupov MN,Antson AA,Dodson EJ,Dodson GG,Dementieva IS,Zakomirdina LN,Wilson KS,Dauter Z,Lebedev AA,Harutyunyan EH

更新日期：1998-02-27 00:00:00

abstract：:Phylogenetic trees were derived for the Alphaherpesvirinae subfamily of the Herpesviridae using molecular sequences. Sequences from the families of genes encoding glycoprotein B, thymidine kinase, S region protein kinase, immediate-early transcriptional regulator IE175 and ribonucleotide reductase large subunit were e...

journal_title：Journal of molecular biology

authors： McGeoch DJ,Cook S

更新日期：1994-04-22 00:00:00

abstract：:The expression of intracellular antibodies (intrabodies) in eukaryotic cells has provided a powerful tool to manipulate microbial and cellular signaling pathways in a highly precise manner. However, there have been several technical issues that have restricted their more widespread use. In particular, single-chain ant...

journal_title：Journal of molecular biology

authors： Gennari F,Mehta S,Wang Y,St Clair Tallarico A,Palu G,Marasco WA

更新日期：2004-01-02 00:00:00

abstract：:Phenol-soluble modulin α3 (PSMα3) is a functional amyloid secreted by the pathogenic bacterium Staphylococcus aureus. This 22-residue peptide serves as a key virulence determinant, toxic to human cells via the formation of unique cross-α amyloid-like fibrils. We demonstrate that bilayer vesicles accelerated PSMα3 fibr...

journal_title：Journal of molecular biology

authors： Malishev R,Tayeb-Fligelman E,David S,Meijler MM,Landau M,Jelinek R

更新日期：2018-05-11 00:00:00

abstract：:The role of a polypeptide loop in tyrosine hydroxylase (TyrH) whose homolog in phenylalanine hydroxylase (PheH) takes on a different conformation when substrates are bound has been studied using site-directed mutagenesis. The loop spans positions 177 to 191; alanine was introduced into those positions, introducing one...

journal_title：Journal of molecular biology

authors： Daubner SC,McGinnis JT,Gardner M,Kroboth SL,Morris AR,Fitzpatrick PF

更新日期：2006-06-02 00:00:00

abstract：:The mobile element IS30 has dual target specificity, since it can integrate adjacent to the inverted repeat (IR) of another IS30 copy or into hot-spot sequences characterized by a well-defined consensus showing no similarity to the ends of the element. The result of such integrations into these targets is different, a...

journal_title：Journal of molecular biology

authors： Olasz F,Fischer T,Szabó M,Nagy Z,Kiss J

更新日期：2003-12-12 00:00:00

abstract：:EzMol is a molecular visualization Web server in the form of a software wizard, located at http://www.sbg.bio.ic.ac.uk/ezmol/. It is designed for easy and rapid image manipulation and display of protein molecules, and is intended for users who need to quickly produce high-resolution images of protein molecules but do ...

journal_title：Journal of molecular biology

authors： Reynolds CR,Islam SA,Sternberg MJE

更新日期：2018-07-20 00:00:00

abstract：:The ribonucleoprotein (RNP) domain is one of the most common eukaryotic protein domains, and is found in many proteins involved in recognition of a wide variety of RNAs. Two structures of RNA complexes of human U1A protein have revealed important aspects of RNP-RNA recognition, but have also raised intriguing question...

journal_title：Journal of molecular biology

authors： Mittermaier A,Varani L,Muhandiram DR,Kay LE,Varani G

更新日期：1999-12-10 00:00:00

abstract：:We have investigated the effects of Co(NH3)6(3+), an analog of hexahydrated Mg2+, on folding and catalysis of the Neurospora VS ribozyme. Most of the metal ion-induced changes detected by chemical modification structure probing in either metal ion are similar, but occur at approximately 33-fold lower concentrations of...

journal_title：Journal of molecular biology

authors： Maguire JL,Collins RA

更新日期：2001-05-25 00:00:00

abstract：:The prokaryotic transcription factors GreA and GreB are involved in the regulation of transcript elongation by RNA polymerase (RNAP). Their known activities include suppression of transcription arrest, enhancement of transcription fidelity, and facilitation of the transition from abortive initiation to productive elon...

journal_title：Journal of molecular biology

authors： Koulich D,Nikiforov V,Borukhov S

更新日期：1998-02-20 00:00:00

abstract：:Here, we present the basic structural properties and the thermodynamic description of a previously unknown alkali molten globule state of horse "ferricytochrome c". Both sodium and guanidinium cations stabilize the alkali-denatured state at pH 13, presumably by a charge screening mechanism. The Na(+)-stabilized confor...

journal_title：Journal of molecular biology

authors： Kumar R,Prabhu NP,Rao DK,Bhuyan AK

更新日期：2006-12-01 00:00:00

abstract：:Bloom syndrome protein forms an oligomeric ring structure and belongs to a group of DNA helicases showing extensive homology to the Escherichia coli DNA helicase RecQ, a suppressor of illegitimate recombination. After over-production in E.coli, we have purified the RecQ core of BLM consisting of the DEAH, RecQ-Ct and ...

journal_title：Journal of molecular biology

authors： Janscak P,Garcia PL,Hamburger F,Makuta Y,Shiraishi K,Imai Y,Ikeda H,Bickle TA

更新日期：2003-06-27 00:00:00

abstract：:Heat capacity curves as obtained from differential scanning calorimetry are an outstanding source for molecular information on protein folding and ligand-binding energetics. However, deconvolution of C(p) data of proteins in the presence of ligands can be compromised by indeterminacies concerning the correct choice of...

journal_title：Journal of molecular biology

authors： Rösgen J,Hinz HJ

更新日期：2001-03-02 00:00:00

abstract：:Electron crystallography offers an increasingly viable alternative to X-ray crystallography for structure determination, especially for membrane proteins. The methodology has been developed and successfully applied to 2D crystals; however, well-ordered thin, 3D crystals are often produced during crystallization trials...

journal_title：Journal of molecular biology

authors： Shi D,Lewis MR,Young HS,Stokes DL

更新日期：1998-12-18 00:00:00

abstract：:Hsp40-like co-chaperones are ubiquitous enzymes that stimulate the protein refolding activity of Hsp70 family chaperones. They are widespread in prokaryotic and eukaryotic systems. In bacteria, the best characterized co-chaperone is the Escherichia coli DnaJ protein. Many γ-proteobacteria encode a functional homologue...

journal_title：Journal of molecular biology

authors： Chintakayala K,Grainger DC

更新日期：2011-08-12 00:00:00

abstract：:Crystals of a chymotrypsin inhibitor from Erythrina caffra seeds have been grown out of lithium sulfate, by the hanging drop method of vapor diffusion. The crystals belong to the rhombohedral space group R32, with a = 67.2 A and alpha = 99.4 degrees, and diffract to 3 A resolution. ...

journal_title：Journal of molecular biology

authors： Shieh HS,Leimgruber NK,Palmier MO,Wun TC,Leimgruber RM,Abdel-Meguid SS

更新日期：1990-03-05 00:00:00

abstract：:Src homology-2 (SH2) domains are protein modules found within a wide variety of cytoplasmic signalling molecules that bind with high affinity to phosphotyrosyl-containing protein sequences. In order to develop SH2 inhibitors that contain phosphotyrosyl analogues resistant to cellular phosphatases, we have solved the c...

journal_title：Journal of molecular biology

authors： Mikol V,Baumann G,Keller TH,Manning U,Zurini MG

更新日期：1995-02-17 00:00:00

abstract：:Recent studies indicate that binding of the urokinase-type plasminogen activator (uPA) to its high-affinity receptor (uPAR) orchestrates uPAR interactions with other cellular components that play a pivotal role in diverse (patho-)physiological processes, including wound healing, angiogenesis, inflammation, and cancer ...

journal_title：Journal of molecular biology

authors： Barinka C,Parry G,Callahan J,Shaw DE,Kuo A,Bdeir K,Cines DB,Mazar A,Lubkowski J

更新日期：2006-10-20 00:00:00