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Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy.

Abstract：

:The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints on the geometry of the metal-cysteine clusters determined by heteronuclear correlation spectroscopy, and dihedral angle constraints derived from both coupling constants and nuclear Overhauser effects. The structure calculations were performed with the program DISMAN. As in previous studies with rabbit liver metallothionein-2a, the structure calculations were performed separately for the alpha and beta-domains containing the 4 and 3-metal clusters, respectively, since no interdomain constraints were found. For both domains, the global polypeptide fold, the location of polypeptide secondary structure elements, the architecture of the metal-sulfur cluster and the local chirality of the metal co-ordination are very similar to the solution structure of rabbit metallothionein-2a, but show considerable difference relative to the crystal structure of rat metallothionein-2.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Schultze P,Wörgötter E,Braun W,Wagner G,Vasák M,Kägi JH,Wüthrich K

doi

10.1016/0022-2836(88)90106-4

subject

Has Abstract

pub_date

1988-09-05 00:00:00

pages

251-68

issue

1

eissn

0022-2836

issn

1089-8638

pii

0022-2836(88)90106-4

journal_volume

203

pub_type

杂志文章

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