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The elastic I-band region of titin is assembled in a "modular" fashion by weakly interacting Ig-like domains.

Abstract：

:The vertebrate striated muscle protein titin is thought to play a critical rôle in myofibril assembly and passive tension. The recently determined complete primary structure of titin revealed a modular architecture that opens the way to a structural characterisation and the understanding of essential properties of this molecule through dissection into units that are structurally and/or functionally relevant. To understand the assembly process of titin, and ultimately the molecular basis of its elastic behaviour, we studied the thermodynamic properties of module pairs, the smallest structural unit that includes a module-module interface. Thus, selected module pairs and their component single modules from the I-band part of the titin molecule were expressed in Escherichia coli and their heat-induced and denaturant-induced unfolding was investigated with a combination of techniques (circular dichroism, fluorescence spectroscopy and nuclear magnetic resonance). The stabilities of single modules and pairs were determined from denaturation experiments. The module interface was also modelled on the basis of the sequence alignment of all approximately 40 immunoglobulin like modules from the I-band and the known structure of one of them. Our results show that all modules and module pairs examined are independently folded in solution. When covalently linked, although weakly interacting, they still behave as autonomous co-operative units upon unfolding. These observations lead us to suggest that folding of titin in vitro is a hierarchical event and that weak interactions between its adjacent modules must only partly account for its presumed elastic function.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Politou AS,Gautel M,Improta S,Vangelista L,Pastore A

doi

10.1006/jmbi.1996.0050

subject

Has Abstract

pub_date

1996-02-02 00:00:00

pages

604-16

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(96)90050-9

journal_volume

255

pub_type

杂志文章

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