Nature of recombination involved in excision and rearrangement of human repetitive DNA.

abstract：:Elastomeric proteins are molecular springs found not only in a variety of biological machines and tissues, but also in biomaterials of superb mechanical properties. Regulating the mechanical stability of elastomeric proteins is not only important for a range of biological processes, but also critical for the use of en...

journal_title：Journal of molecular biology

authors： Cao Y,Yoo T,Zhuang S,Li H

更新日期：2008-05-16 00:00:00

abstract：:Transverse sections (100 to 140 nm thick) of the flight muscles of the fleshfly Phormia terrae-novae and the housefly Musca domestica were studied. The images of 56 tubular myosin filaments of the fleshfly and 62 filaments of the housefly were digitized and computer processed by rotational averaging. The rotational po...

journal_title：Journal of molecular biology

authors： Beinbrech G,Ashton FT,Pepe FA

更新日期：1988-06-05 00:00:00

abstract：:The contribution of the tRNA "core" to aminoacylation is beginning to be recognized. One example is the core region of Escherichia coli tRNA(Cys), which has been shown by biochemical studies to be important for aminoacylation. This core has several layers of unusual base-pairs, which are revealed by the recent crystal...

journal_title：Journal of molecular biology

authors： Hamann CS,Hou YM

更新日期：2000-01-28 00:00:00

abstract：:Sir2 is a protein deacetylase that mediates transcriptional silencing at the HM loci, telomeres, and rDNA repeats in yeast. To identify functionally significant regions of the Sir2 protein, we have characterized two types of mutations. First, we used random mutagenesis to create temperature-sensitive alleles of the SI...

journal_title：Journal of molecular biology

authors： Hickman M,McCullough K,Woike A,Raducha-Grace L,Rozario T,Dula ML,Anderson E,Margalit D,Holmes SG

更新日期：2007-04-13 00:00:00

abstract：:Polar residues are capable of mediating the association of membrane-embedded helices through the formation of side-chain/side-chain inter-helical hydrogen bonds. However, the extent to which native van der Waals packing of the residues surrounding the polar locus can enhance, or interfere with, the interaction of pola...

journal_title：Journal of molecular biology

authors： Dawson JP,Melnyk RA,Deber CM,Engelman DM

更新日期：2003-08-01 00:00:00

abstract：:Complex gene regulation systems ensure the maintenance of cellular identity during early development in mammals. Eukaryotic small RNAs have emerged as critical players in RNA interference (RNAi) by mediating gene silencing during embryonic stem cell self-renewal. Most of the proteins involved in the biogenesis of smal...

journal_title：Journal of molecular biology

authors： Bodak M,Cirera-Salinas D,Luitz J,Ciaudo C

更新日期：2017-05-19 00:00:00

abstract：:Current data suggest that globular domains may form amyloids via different mechanisms. Nevertheless, there are indications that the initiation of the process takes place invariably in the less stable segments of a protein domain. We have studied the sequence and structural conservation of beta(2)-microglobulin that de...

journal_title：Journal of molecular biology

authors： Benyamini H,Gunasekaran K,Wolfson H,Nussinov R

更新日期：2003-06-27 00:00:00

abstract：:Annexin A8 is a relatively infrequent and poorly studied member of this large family of calcium-binding and membrane-binding proteins. It is, however, associated with a specific disease, acute promyelocytic leukemia. We have solved its three-dimensional structure, which includes a moderately long and intact N terminus...

journal_title：Journal of molecular biology

authors： Réty S,Sopková-de Oliveira Santos J,Dreyfuss L,Blondeau K,Hofbauerová K,Raguénès-Nicol C,Kerboeuf D,Renouard M,Russo-Marie F,Lewit-Bentley A

更新日期：2005-02-04 00:00:00

abstract：:Protein interactions play a crucial role among the different functions of a cell and are central to our understanding of cellular processes both in health and disease. Here we present Galaxy InteractoMIX (http://galaxy.interactomix.com), a platform composed of 13 different computational tools each addressing specific ...

journal_title：Journal of molecular biology

authors： Mirela-Bota P,Aguirre-Plans J,Meseguer A,Galletti C,Segura J,Planas-Iglesias J,Garcia-Garcia J,Guney E,Oliva B,Fernandez-Fuentes N

更新日期：2020-09-23 00:00:00

abstract：:The papillomavirus E1 protein is essential for viral DNA replication, and phosphorylation of E1 appears to regulate protein function and DNA replication. Serine 584 of bovine papillomavirus E1 is in a conserved motif resembling a CK2 consensus site, and is phosphorylated by CK2 in vitro. Mutation of serine 584 to alan...

journal_title：Journal of molecular biology

authors： Lentz M,Zanardi T,Filzen R,Carter J,Hella M

更新日期：2002-02-22 00:00:00

abstract：:The crystal structure of the ferredoxin:NADP+ reductase (FNR) from the cyanobacterium Anabaena PCC 7119 has been determined at 2.6 A resolution by multiple isomorphous replacement and refined using 15.0 A to 1.8 A data, collected at 4 degrees C, to an R-factor of 0.172. The model includes 303 residues, the flavin aden...

journal_title：Journal of molecular biology

authors： Serre L,Vellieux FM,Medina M,Gomez-Moreno C,Fontecilla-Camps JC,Frey M

更新日期：1996-10-18 00:00:00

abstract：:The PstI fragment (65.5% to 77.4%) of coliphage 186, known genetically to encode the major control genes, has been sequenced, and an analysis performed to assess coding capacity, transcription-translation signals, and to identify any other significant features. Our analysis indicates that the region encodes: seven gen...

journal_title：Journal of molecular biology

authors： Kalionis B,Dodd IB,Egan JB

更新日期：1986-09-20 00:00:00

abstract：:Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis proposed to form specific trimer-of-trimers structures, acts as a molecular chaperone in vitro. The assembly and re-assembly mechanisms of this oligomeric protein were studied and compared using in vitro transcription/translation and denaturization/re...

journal_title：Journal of molecular biology

authors： Abulimiti A,Fu X,Gu L,Feng X,Chang Z

更新日期：2003-02-28 00:00:00

abstract：:We have evaluated the thermodynamic parameters associated with cooperative cofilin binding to actin filaments, accounting for contributions of ion-linked equilibria, and determined the kinetic basis of cooperative cofilin binding. Ions weaken non-contiguous (isolated, non-cooperative) cofilin binding to an actin filam...

journal_title：Journal of molecular biology

authors： Cao W,Goodarzi JP,De La Cruz EM

更新日期：2006-08-11 00:00:00

abstract：:The Monte Carlo estimate of the p value of the hypergeometric test is described and advocated for the testing of the hypothesis that different treatments induce the same mutational spectrum. The hypergeometric test is a generalization of Fisher's "exact" test for tables with more than two rows and two columns. Use of ...

journal_title：Journal of molecular biology

authors： Adams WT,Skopek TR

更新日期：1987-04-05 00:00:00

abstract：:YibK is a 160 residue homodimeric protein belonging to the SPOUT class of methyltransferases. Proteins in this group all display a unique topological feature; the backbone polypeptide chain folds to form a deep trefoil knot. Such knotted structures were completely unpredicted, it being thought impossible for a protein...

journal_title：Journal of molecular biology

authors： Mallam AL,Jackson SE

更新日期：2005-03-11 00:00:00

abstract：:Single amino acid residue substitutions rarely destroy the structural integrity of proteins. Substitution of glycine residues, however, is among the few sorts of alterations that can have such an effect. Here, we seek to understand what accounts for the extreme functional impairment of the bacterial ribonuclease barna...

journal_title：Journal of molecular biology

authors： Axe DD,Foster NW,Fersht AR

更新日期：1999-03-12 00:00:00

abstract：:We have examined the chromatin organization of a cluster of transfer RNA genes at the cytogenic locus 90BC on the right arm of the third Drosophila melanogaster chromosome. We find that the internal promoter sequences are preferentially exposed to micrococcal nuclease and DNase I in chromatin. Moreover, these exposed ...

journal_title：Journal of molecular biology

authors： DeLotto R,Schedl P

更新日期：1984-11-15 00:00:00

abstract：:The mRNA-capping process starts with the conversion of a 5'-triphosphate end into a 5'-diphosphate by an RNA triphosphatase, followed by the addition of a guanosine monophosphate unit in a 5'-5' phosphodiester bond by a guanylyltransferase. Methyltransferases are involved in the third step of the process, transferring...

journal_title：Journal of molecular biology

authors： Bollati M,Milani M,Mastrangelo E,Ricagno S,Tedeschi G,Nonnis S,Decroly E,Selisko B,de Lamballerie X,Coutard B,Canard B,Bolognesi M

更新日期：2009-01-09 00:00:00

abstract：:To address the mechanism of thermosensing and its implications for molecular engineering, we previously deconstructed the functional components of the bacterial thermosensor DesK, a histidine kinase with a five-span transmembrane domain that detects temperature changes. The system was first simplified by building a se...

journal_title：Journal of molecular biology

authors： Inda ME,Vazquez DB,Fernández A,Cybulski LE

更新日期：2019-03-01 00:00:00

abstract：:Huntington's disease (HD) is an inherited progressive neurodegenerative disorder caused by a pathological CAG trinucleotide repeat expansion in the large multi-exon gene, huntingtin (HTT). Although multiple pathogenic mechanisms have been proposed for HD, there is increasing interest in the RNA processing of the HTT g...

journal_title：Journal of molecular biology

authors： Hughes AC,Mort M,Elliston L,Thomas RM,Brooks SP,Dunnett SB,Jones L

更新日期：2014-04-03 00:00:00

abstract：:Bacterial microcompartments (MCPs) are the simplest organelles known. They function to enhance metabolic pathways by confining several related enzymes inside an all-protein envelope called the shell. In this study, we investigated the factors that govern MCP assembly by performing scanning mutagenesis on the surface r...

journal_title：Journal of molecular biology

authors： Sinha S,Cheng S,Sung YW,McNamara DE,Sawaya MR,Yeates TO,Bobik TA

更新日期：2014-06-12 00:00:00

abstract：:Ca2+ concentration jumps for the activation of Ca2+-dependent ion channels or transporters can be obtained either by fast solution exchange or by the use of caged Ca2+. Here, we report on an alternate optogenetic method for the activation of Ca2+ and voltage-dependent large conductance (BK) potassium channels. This wa...

journal_title：Journal of molecular biology

authors： Mager T,Wood PG,Bamberg E

更新日期：2017-03-24 00:00:00

abstract：:The enzyme mercuric ion reductase MerA is the central component of bacterial mercury resistance encoded by the mer operon. Many MerA proteins possess metallochaperone-like N-terminal domains (NmerA) that can transfer Hg(2+) to the catalytic core domain (Core) for reduction to Hg(0). These domains are tethered to the h...

journal_title：Journal of molecular biology

authors： Johs A,Harwood IM,Parks JM,Nauss RE,Smith JC,Liang L,Miller SM

更新日期：2011-10-28 00:00:00

abstract：:When theoretical methods are used to predict the properties of a given system, such as the effects of the substitution of a specific amino acid on the activity or stability of a protein as a whole, the accuracy of the prediction is directly dependent on the validity of the underlying model. A common error, however, is...

journal_title：Journal of molecular biology

authors： van Gunsteren WF,Mark AE

更新日期：1992-09-20 00:00:00

abstract：:The endA gene encoding the membrane nuclease of Streptococcus pneumoniae, which is necessary for DNA uptake in genetic transformation, was cloned in a streptococcal vector. This was accomplished by insertional mutagenesis of the gene, cloning of the mutant allele, and substitution of the wild-type allele by chromosoma...

journal_title：Journal of molecular biology

authors： Puyet A,Greenberg B,Lacks SA

更新日期：1990-06-20 00:00:00

abstract：:We report the preparation, crystallization and preliminary X-ray crystallographic study of the Fab fragment from a heteroclitic murine (BALB/c) monoclonal anti-hen egg-white lysozyme antibody complexed with a heterologous antigen, pheasant lysozyme. The complex between the heterologous antigen and the antibody has bee...

journal_title：Journal of molecular biology

authors： Guillon V,Alzari PM,Poljak RJ

更新日期：1987-09-20 00:00:00

abstract：:Neurodegenerative disorders associated with protein misfolding are fatal diseases that are caused by fibrillation of endogenous proteins such as α-synuclein (α-syn) in Parkinson's disease (PD) or amyloid-β in Alzheimer's disease. Fibrils of α-syn are a major pathological hallmark of PD and certain aggregation intermed...

journal_title：Journal of molecular biology

authors： Wördehoff MM,Bannach O,Shaykhalishahi H,Kulawik A,Schiefer S,Willbold D,Hoyer W,Birkmann E

更新日期：2015-03-27 00:00:00

abstract：:Building on the substantial progress that has been made in using free energy perturbation (FEP) methods to predict the relative binding affinities of small molecule ligands to proteins, we have previously shown that results of similar quality can be obtained in predicting the effect of mutations on the binding affinit...

journal_title：Journal of molecular biology

authors： Clark AJ,Negron C,Hauser K,Sun M,Wang L,Abel R,Friesner RA

更新日期：2019-03-29 00:00:00

abstract：:Cyclin K and the closely related cyclins T1, T2a, and T2b interact with cyclin-dependent kinase 9 (CDK9) forming multiple nuclear complexes, referred to collectively as positive transcription elongation factor b (P-TEFb). Through phosphorylation of the C-terminal domain of the RNA polymerase II largest subunit, distin...

journal_title：Journal of molecular biology

authors： Baek K,Brown RS,Birrane G,Ladias JA

更新日期：2007-02-16 00:00:00