The crystal structure of annexin A8 is similar to that of annexin A3.

abstract：:We have conducted a detailed kinetic and thermodynamic analysis of open complex formation between Escherichia coli RNA polymerase and the A1 promoter from bacteriophage T7 by monitoring alterations in the intrinsic protein fluorescence of RNA polymerase in stopped-flow kinetic studies. The stopped-flow kinetic data ar...

journal_title：Journal of molecular biology

authors： Johnson RS,Chester RE

更新日期：1998-10-23 00:00:00

abstract：:The recA protein from Proteus mirabilis, which is homologous to the Escherichia coli protein, forms crystals in the orthorhombic space group P2(1)2(1)2(1). There are two 38,000 molecular weight subunits in the asymmetric unit and the unit cell dimensions are a = 57.5, b = 127.0 and c = 157.0 A. ...

journal_title：Journal of molecular biology

authors： Weber IT,Steitz TA

更新日期：1986-03-05 00:00:00

abstract：:The intracellular ileal lipid binding proteins (ILBPs) are involved in the transport and enterohepatic circulation of bile acids. ILBPs from different species show high sequence and structural homology and have been shown to bind multiple bile acid ligands with differing degrees of selectivity and positive co-operativ...

journal_title：Journal of molecular biology

authors： Kouvatsos N,Thurston V,Ball K,Oldham NJ,Thomas NR,Searle MS

更新日期：2007-08-31 00:00:00

abstract：:alpha-Synuclein is an intrinsically disordered protein that appears in aggregated forms in the brains of patients with Parkinson's disease. The conversion from monomer to aggregate is complex, and aggregation rates are sensitive to changes in amino acid sequence and environmental conditions. It has previously been obs...

journal_title：Journal of molecular biology

authors： Wu KP,Weinstock DS,Narayanan C,Levy RM,Baum J

更新日期：2009-08-28 00:00:00

abstract：:The actin cytoskeleton is a complex network controlled by a vast array of intricately regulated actin-binding proteins. Human plastins (PLS1, PLS2, and PLS3) are evolutionary conserved proteins that non-covalently crosslink actin filaments into tight bundles. Through stabilization of such bundles, plastins contribute,...

journal_title：Journal of molecular biology

authors： Schwebach CL,Agrawal R,Lindert S,Kudryashova E,Kudryashov DS

更新日期：2017-08-04 00:00:00

abstract：:The great diversity of RNA biological functions has led to widespread interest in RNA structure. Advances in synthetic and spectroscopic techniques have recently allowed the extension of NMR methods of structure determination to RNA structures of significant size and increased biological significance. However, it has ...

journal_title：Journal of molecular biology

authors： Allain FH,Varani G

更新日期：1997-03-28 00:00:00

abstract：:In order to determine how much of the natural sequence is required for function of the lambda tR1 rho-dependent terminator, and to determine the minimum length required, we made two deletion series of a tR1 derivative that contains mostly foreign DNA, encoding C-rich RNA, substituted for the natural upstream sequences...

journal_title：Journal of molecular biology

authors： Hart CM,Roberts JW

更新日期：1994-04-01 00:00:00

abstract：:Proteolysis of rat liver chromatin by the Arg-C peptidase, clostripain, is characterized by a progressive fragmentation of the N-terminal segments of the four core histones H2A, H2B, H3 and H4, until a well-defined limit digest is reached. This work addresses the case of histone H4. Two intermediate proteolytic sites ...

journal_title：Journal of molecular biology

authors： Banères JL,Essalouh L,Jariel-Encontre I,Mesnier D,Garrod S,Parello J

更新日期：1994-10-14 00:00:00

abstract：:Eukaryotic aminoacyl-tRNA synthetases are usually organized into high-molecular-weight complexes, the structure and function of which are poorly understood. We have previously described a yeast complex containing two aminoacyl-tRNA synthetases, methionyl-tRNA synthetase and glutamyl-tRNA synthetase, and one noncatalyt...

journal_title：Journal of molecular biology

authors： Karanasios E,Simader H,Panayotou G,Suck D,Simos G

更新日期：2007-12-07 00:00:00

abstract：:The aim of this paper is to clarify the mechanism of gel electrophoresis of DNA under constant-field conditions. We have conducted a large number of experiments on double-stranded DNA varying in length between approximately 10 and approximately 50,000 base-pairs, in both agarose and polyacrylamide gels ranging from 0....

journal_title：Journal of molecular biology

authors： Calladine CR,Collis CM,Drew HR,Mott MR

更新日期：1991-10-05 00:00:00

abstract：:We propose a general classification scheme for loops, aperiodic segments of protein structure. In an effort to avoid the geometric complexity created by non-repeating phi psi angles, a morphologic definition that focuses upon the linearity and planarity of loops is utilized. Out of 432 loops (4 to 20 residues in lengt...

journal_title：Journal of molecular biology

authors： Ring CS,Kneller DG,Langridge R,Cohen FE

更新日期：1992-04-05 00:00:00

abstract：:The crystal structure of the glutamine-binding protein (GlnBP) from Escherichia coli in a ligand-free "open" conformational state has been determined by isomorphous replacement methods and refined to an R-value of 21.4% at 2.3 A resolution. There are two molecules in the asymmetric unit, related by pseudo 4-fold screw...

journal_title：Journal of molecular biology

authors： Hsiao CD,Sun YJ,Rose J,Wang BC

更新日期：1996-09-20 00:00:00

abstract：:Collagen fibrils are the principle source of mechanical strength in the mutable dermis of the sea cucumber Cucumaria frondosa. To obtain information about the mechanism by which collagen molecules self-assemble into fibrils, we have isolated single intact fibrils with lengths in the range 14-444 microm. These fibrils ...

journal_title：Journal of molecular biology

authors： Trotter JA,Chapman JA,Kadler KE,Holmes DF

更新日期：1998-12-18 00:00:00

abstract：:When influenza A virus infects host cells, its integral matrix protein M2 forms a proton-selective channel in the viral envelope. Although X-ray crystallography and NMR studies using fragment peptides have suggested that M2 stably forms a tetrameric channel irrespective of pH, the oligomeric states of the full-length ...

journal_title：Journal of molecular biology

authors： Kawano K,Yano Y,Matsuzaki K

更新日期：2014-07-15 00:00:00

abstract：:The DNA oligonucleotide d(GGTTGGTGTGGTTGG) (thrombin aptamer) binds to thrombin and inhibits its enzymatic activity in the chain of reactions that lead to blood clotting. Two-dimensional 1H NMR studies indicate that the oligonucleotide forms a folded structure in solution, composed of two guanine quartets connected by...

journal_title：Journal of molecular biology

authors： Schultze P,Macaya RF,Feigon J

更新日期：1994-02-04 00:00:00

abstract：:Type IV pili are multifunctional filaments displayed on many bacterial pathogens. Members of the Type IVa pilus subclass are found on a diverse group of human pathogens, whereas Type IVb pili are found almost exclusively on enteric bacteria. The Type IVa and IVb subclasses are distinguished by differences in the pilin...

journal_title：Journal of molecular biology

authors： Li J,Egelman EH,Craig L

更新日期：2012-04-20 00:00:00

abstract：:Drosophila alcohol dehydrogenase (DADH) is an NAD+-dependent enzyme that catalyzes the oxidation of alcohols to aldehydes/ketones and that is also able to further oxidize aldehydes to their corresponding carboxylic acids. The structure of the ternary enzyme-NADH-acetate complex of the slow alleloform of Drosophila mel...

journal_title：Journal of molecular biology

authors： Benach J,Winberg JO,Svendsen JS,Atrian S,Gonzàlez-Duarte R,Ladenstein R

更新日期：2005-01-21 00:00:00

abstract：:In an X-ray diffraction study using the method of multiple isomorphous replacement, the structure of aspartate carbamoyltransferase (EC 2.1.3.2) complexed with the bisubstrate analog N-(phosphonacetyl)-L-aspartate (PALA) has been solved to 2.5 A. Ten rounds of model building and 123 cycles of restrained reciprocal spa...

journal_title：Journal of molecular biology

authors： Krause KL,Volz KW,Lipscomb WN

更新日期：1987-02-05 00:00:00

abstract：:GTP and Ca2+, two well-known modulators of intracellular signaling pathways, control a structural/functional switch between two vital and mutually exclusive activities, cross-linking and Galpha activity, in the same enzyme. The enzyme, a brain-derived tissue-type transglutaminase (TGase), was recently cloned by us in ...

journal_title：Journal of molecular biology

authors： Monsonego A,Friedmann I,Shani Y,Eisenstein M,Schwartz M

更新日期：1998-10-02 00:00:00

abstract：:The transcription of the beta-globin genes in mouse erythroleukemia cells has been examined by hybridizing labeled RNA obtained from isolated nuclei after chain elongation in the presence of [alpha-32P]UTP. There is induction of at least 30-fold of beta maj globin transcription after cells are treated with either dime...

journal_title：Journal of molecular biology

authors： Salditt-Georgieff M,Sheffery M,Krauter K,Darnell JE Jr,Rifkind R,Marks PA

更新日期：1984-02-05 00:00:00

abstract：:Hepatitis A virus (HAV) 3C proteinase is a member of the picornain cysteine proteases responsible for the processing of the viral polyprotein, a function essential for viral maturation and infectivity. This and its structural similarity to other 3C and 3C-like proteases make it an attractive target for the development...

journal_title：Journal of molecular biology

authors： Yin J,Bergmann EM,Cherney MM,Lall MS,Jain RP,Vederas JC,James MN

更新日期：2005-12-09 00:00:00

abstract：:Recombination between RNAs associated with turnip crinkle virus is thought to occur during plus-strand synthesis at motifs resembling the 5'-ends of genomic, subgenomic and satellite RNAs. Common structural regions encompassing the motifs have been found for major crossover sites on two different minus-strand template...

journal_title：Journal of molecular biology

authors： Carpenter CD,Oh JW,Zhang C,Simon AE

更新日期：1995-02-03 00:00:00

abstract：:We have developed a procedure to predict the peptide binding specificity of an SH3 domain from its sequence. The procedure utilizes information extracted from position-specific contacts derived from six SH3/peptide or SH3/protein complexes of known structure. The framework of SH3/peptide contacts defined on the struct...

journal_title：Journal of molecular biology

authors： Brannetti B,Via A,Cestra G,Cesareni G,Helmer-Citterich M

更新日期：2000-04-28 00:00:00

abstract：:The Sto12a protein, from the thermoacidophilic archaeon Sulfolobus tokodaii, has been identified as a small putative DNA-binding protein. Most of the proteins with a high level of amino acid sequence homology to this protein are derived from members of the Sulfolobaceae family, including a transcriptional regulator. W...

journal_title：Journal of molecular biology

authors： Shinkai A,Sekine S,Urushibata A,Terada T,Shirouzu M,Yokoyama S

更新日期：2007-10-05 00:00:00

abstract：:Mechanisms by which many N-terminal propeptides facilitate folding of proteins are unknown. The maturation of such proteins from their precursors involve three steps, namely: (1) folding of the precursor, (2) autoprocessing of the propeptide from the N terminus and (3) degradation of the cleaved propeptide. Using subt...

journal_title：Journal of molecular biology

authors： Shinde U,Inouye M

更新日期：1995-09-08 00:00:00

abstract：:Large RNAs often utilize GNRA tetraloops as structural elements to stabilize the overall tertiary fold. These tetraloop-receptor (TR) interactions have a conserved geometry in which the tetraloop docks into the receptor at an angle of ~15° from the helix containing the receptor. Here, we show that the conserved GUAAY ...

journal_title：Journal of molecular biology

authors： Chan RT,Keating KS,Go MC,Toor N

更新日期：2016-12-04 00:00:00

abstract：:KorA and KorB proteins of IncP1 plasmid RK2 are encoded in the central control region (ccr) of the plasmid and act as global regulators of plasmid genes for replication, transfer and stable inheritance. KorA represses seven promoters on RK2, by binding to a defined operator site, OA, which always occurs in promoter re...

journal_title：Journal of molecular biology

authors： Kostelidou K,Jones AC,Thomas CM

更新日期：1999-06-04 00:00:00

abstract：:A normal mode and energy minimization of ras p21 is used to determine the flexibility of the protein and the origin of the conformational differences between GTP and GDP-bound forms. To preserve the integrity of the structures, a hydration shell of water molecules was included as part of the system. Certain low-freque...

journal_title：Journal of molecular biology

authors： Ma J,Karplus M

更新日期：1997-11-21 00:00:00

abstract：:As part of an overall effort to map the energetic landscape of the base excision repair pathway, we report the first thermodynamic characterization of repair enzyme binding to lesion-containing duplexes. Isothermal titration calorimetry (ITC) in conjunction with spectroscopic measurements and protease protection assay...

journal_title：Journal of molecular biology

authors： Minetti CA,Remeta DP,Zharkov DO,Plum GE,Johnson F,Grollman AP,Breslauer KJ

更新日期：2003-05-16 00:00:00

abstract：:Amber suppressors previously isolated from the yeast Saccharomyces cerevisiae and belonging to the same phenotypic class (Liebman et al., 1976) were assigned to nine different linkage groups named SUP52 through SUP60. One of these suppressors, SUP52, had been shown to cause the insertion of leucine and had been geneti...

journal_title：Journal of molecular biology

authors： Liebman SW,Srodulski Z,Reed CR,Stewart JW,Sherman F,Brennan G

更新日期：1984-09-15 00:00:00