Preliminary crystallographic study of a complex between an heteroclitic anti-hen egg-white lysozyme antibody and the heterologous antigen pheasant egg-white lysozyme.

abstract：:The serendipitous observation of a C-H cdots, three dots, centered O hydrogen bond mediated polypeptide chain reversal in synthetic peptide helices has led to a search for the occurrence of a similar motif in protein structures. From a dataset of 634 proteins, 1304 helices terminating in a Schellman motif have been ex...

journal_title：Journal of molecular biology

authors： Madan Babu M,Kumar Singh S,Balaram P

更新日期：2002-09-27 00:00:00

abstract：:Phage Cp-1 from Streptoccocus pneumoniae makes use of a protein-priming mechanism to start replication of its linear DNA: the first reaction consists of the addition of 5' dAMP to a molecule of the primer protein, an initiation event occurring at both DNA ends. After elongation of the initiation complex, the primer pr...

journal_title：Journal of molecular biology

authors： Martín AC,Blanco L,García P,Salas M,Méndez J

更新日期：1996-07-19 00:00:00

abstract：:The structure of the Escherichia coli gene coding for the metabolically stable 4.5 S RNA has been determined by cloning and DNA sequence analysis. Results from Southern hybridization assays carried out prior to cloning show the 4.5 S DNA to be limited to a single locus in the E. coli K12 genome. A 5.4 X 10(3) base DNA...

journal_title：Journal of molecular biology

authors： Hsu LM,Zagorski J,Fournier MJ

更新日期：1984-09-25 00:00:00

abstract：:The crystal and molecular structure of a triacylglyceride lipase (EC 3.1.1.3) from the fungus Rhizomucor miehei was analyzed using X-ray single crystal diffraction data to 1.9 A resolution. The structure was refined to an R-factor of 0.169 for all available data. The details of the molecular architecture and the cryst...

journal_title：Journal of molecular biology

authors： Derewenda ZS,Derewenda U,Dodson GG

更新日期：1992-10-05 00:00:00

abstract：:Vinculin is a key regulator of the actin cytoskeleton attachment to the cell membrane at cellular adhesion sites, which is crucial for processes such as cell motility and migration, development, survival, and wound healing. Vinculin loss results in embryonic lethality, cardiovascular diseases, and cancer. Its tail dom...

journal_title：Journal of molecular biology

authors： Lee JH,Rangarajan ES,Vonrhein C,Bricogne G,Izard T

更新日期：2012-10-05 00:00:00

abstract：:Recently, we have found a transient intermediate on the folding pathway of src SH3. Intending to investigate the structure of the transient intermediate, we tested a mutant of src SH3, named A45G, using circular dichroism, fluorescence and X-ray solution scattering, and incidentally found that it forms a stable alpha-...

journal_title：Journal of molecular biology

authors： Li J,Matsumura Y,Shinjo M,Kojima M,Kihara H

更新日期：2007-09-21 00:00:00

abstract：:Bacterial microcompartments (BMCs) sequester enzymes from the cytoplasmic environment by encapsulation inside a selectively permeable protein shell. Bioinformatic analyses indicate that many bacteria encode BMC clusters of unknown function and with diverse combinations of shell proteins. The genome of the halophilic m...

journal_title：Journal of molecular biology

authors： Lassila JK,Bernstein SL,Kinney JN,Axen SD,Kerfeld CA

更新日期：2014-05-29 00:00:00

abstract：:The pH and ionic strength dependence of the states of aggregation of brome mosaic virus protein has been investigated by small angle neutron scattering, quasielastic light-scattering, analytical centrifugation and electron microscopy. At pH above neutrality, protein oligomers are found in dynamical equilibrium, compri...

journal_title：Journal of molecular biology

authors： Cuillel M,Zulauf M,Jacrot B

更新日期：1983-03-15 00:00:00

abstract：:The growth of one group of amber mutants of R17 (class A) in non-permissive hosts results in a burst of bacteriophage-like particles, which are defective in that they are non-infectious. Here studies of particles produced by the growth of wild-type R17 and four class A mutants on AB301, a non-permissive Hfr Escherichi...

journal_title：Journal of molecular biology

authors： Argetsinger JE,Gussin GN

更新日期：1966-11-28 00:00:00

abstract：:The structure and dynamics of rat apo-cellular retinol binding protein II (apo-CRBP II) in solution has been determined by multidimensional NMR analysis of uniformly enriched recombinant rat 13C, 15N-apo-CRBP II and 15N-apo-CRBP II. The final ensemble of 24 NMR structures has been calculated from 3274 conformational r...

journal_title：Journal of molecular biology

authors： Lu J,Lin CL,Tang C,Ponder JW,Kao JL,Cistola DP,Li E

更新日期：1999-03-05 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a potent endothelial cell-specific mediator of angiogenesis and vasculogenesis. VEGF is involved pathologically in cancer, proliferative retinopathy and rheumatoid arthritis, and as such represents an important therapeutic target. Three classes of disulfide-constrained pept...

journal_title：Journal of molecular biology

authors： Pan B,Li B,Russell SJ,Tom JY,Cochran AG,Fairbrother WJ

更新日期：2002-02-22 00:00:00

abstract：:We describe the physicochemical characterization of various circular and linear forms of the approximately 60 residue N-terminal Src homology 3 (SH3) domain from the murine c-Crk adapter protein. Structural, dynamic, thermodynamic, kinetic and biochemical studies reveal that backbone circularization does not prevent t...

journal_title：Journal of molecular biology

authors： Camarero JA,Fushman D,Sato S,Giriat I,Cowburn D,Raleigh DP,Muir TW

更新日期：2001-05-18 00:00:00

abstract：:A complete understanding of antibody-antigen association and specificity requires the stereochemical description of both antigen and antibody before and upon complex formation. The structural mechanism involved in the binding of the IgG1 monoclonal antibody E8 to its highly charged protein antigen horse cytochrome c (...

journal_title：Journal of molecular biology

authors： Mylvaganam SE,Paterson Y,Getzoff ED

更新日期：1998-08-14 00:00:00

abstract：:Certain pathogenic trypanosomatids are highly dependent on glycolysis for ATP production, and hence their glycolytic enzymes, including glycerol-3-phosphate dehydrogenase (GPDH), are considered attractive drug targets. The ternary complex structure of Leishmania mexicana GPDH (LmGPDH) with dihydroxyacetone phosphate (...

journal_title：Journal of molecular biology

authors： Choe J,Guerra D,Michels PA,Hol WG

更新日期：2003-05-30 00:00:00

abstract：:HscA, a specialized bacterial Hsp70-class molecular chaperone, interacts with the iron-sulfur cluster assembly protein IscU by recognizing a conserved LPPVK sequence motif. We report the crystal structure of the substrate-binding domain of HscA (SBD, residues 389-616) from Escherichia coli bound to an IscU-derived pep...

journal_title：Journal of molecular biology

authors： Cupp-Vickery JR,Peterson JC,Ta DT,Vickery LE

更新日期：2004-09-24 00:00:00

abstract：:Binding of the TATA-binding protein (TBP) to the "TATA" sequences present in the promoters of eukaryotic class II genes is the first step in the sequential assembly of transcription pre-initiation complexes. Myriad structural changes, including severe bending of the DNA, accompany TBP-TATA complex formation. A detaile...

journal_title：Journal of molecular biology

authors： Parkhurst KM,Richards RM,Brenowitz M,Parkhurst LJ

更新日期：1999-06-25 00:00:00

abstract：:We report here the isolation and primary structure of two genes encoding rat U3 small nuclear RNA. One of the genes encodes U3B RNA; the other encodes an RNA which is almost identical to U3A RNA. Both genes are expressed after microinjection into the nuclei of Xenopus laevis oocytes and can direct the accumulation of ...

journal_title：Journal of molecular biology

authors： Stroke IL,Weiner AM

更新日期：1985-07-20 00:00:00

abstract：:Bulges are common structural motifs in RNA secondary structure and are thought to play important roles in RNA-protein and RNA-drug interactions. Adenosine bases are the most commonly occurring unpaired base in double helical RNA secondary structures. The solution conformation and dynamics of a 25-nucleotide RNA duplex...

journal_title：Journal of molecular biology

authors： Thiviyanathan V,Guliaev AB,Leontis NB,Gorenstein DG

更新日期：2000-07-28 00:00:00

abstract：:Acireductone dioxygenase (ARD) catalyzes different reactions between O2 and 1,2-dihydroxy-3-oxo-5-(methylthio)pent-1-ene (acireductone) depending upon the metal bound in the active site. Ni2+ -ARD cleaves acireductone to formate, CO and methylthiopropionate. If Fe2+ is bound (ARD'), the same substrates yield methylthi...

journal_title：Journal of molecular biology

authors： Ju T,Goldsmith RB,Chai SC,Maroney MJ,Pochapsky SS,Pochapsky TC

更新日期：2006-11-03 00:00:00

abstract：:Current data suggest that globular domains may form amyloids via different mechanisms. Nevertheless, there are indications that the initiation of the process takes place invariably in the less stable segments of a protein domain. We have studied the sequence and structural conservation of beta(2)-microglobulin that de...

journal_title：Journal of molecular biology

authors： Benyamini H,Gunasekaran K,Wolfson H,Nussinov R

更新日期：2003-06-27 00:00:00

abstract：:Protein folding involves the formation of secondary structural elements from the primary sequence and their association with tertiary assemblies. The relation of this primary sequence to a specific folded protein structure remains a central question in structural biology. An increasing body of evidence suggests that v...

journal_title：Journal of molecular biology

authors： Schellenberg MJ,Ritchie DB,Wu T,Markin CJ,Spyracopoulos L,MacMillan AM

更新日期：2010-10-01 00:00:00

abstract：:The capsid (CA) protein plays crucial roles in HIV infection and replication, essential to viral maturation. The absence of high-resolution structural data on unassembled CA hinders the development of antivirals effective in inhibiting assembly. Unlike enzymes that have targetable, functional substrate-binding sites, ...

journal_title：Journal of molecular biology

authors： Du S,Betts L,Yang R,Shi H,Concel J,Ahn J,Aiken C,Zhang P,Yeh JI

更新日期：2011-02-25 00:00:00

abstract：:Mutational experiments show how changes in the hydrophobic cores of proteins affect their stabilities. Here, we estimate these effects computationally, using four-body likelihood potentials obtained by simplicial neighborhood analysis of protein packing (SNAPP). In this procedure, the volume of a known protein structu...

journal_title：Journal of molecular biology

authors： Carter CW Jr,LeFebvre BC,Cammer SA,Tropsha A,Edgell MH

更新日期：2001-08-24 00:00:00

abstract：:Selenophosphate synthetase (SPS) catalyzes the activation of selenide with adenosine 5'-triphosphate (ATP) to generate selenophosphate, the essential reactive selenium donor for the formation of selenocysteine (Sec) and 2-selenouridine residues in proteins and RNAs, respectively. Many SPS are themselves Sec-containing...

journal_title：Journal of molecular biology

authors： Itoh Y,Sekine S,Matsumoto E,Akasaka R,Takemoto C,Shirouzu M,Yokoyama S

更新日期：2009-02-06 00:00:00

abstract：:Chemical modifications, NES-Cys(beta 93), des-Arg(alpha 141), and both modifications on the same molecule, were made to Ni-Fe hybrid hemoglobins, and their effect on individual subunits was investigated by measuring oxygen equilibrium curves, the Fe(II)-N epsilon (His F8) stretching Raman lines, and light-absorption s...

journal_title：Journal of molecular biology

authors： Shibayama N,Morimoto H,Kitagawa T

更新日期：1986-11-20 00:00:00

abstract：:Tumor suppressor p53 plays a central role in tumor suppression. p53 is the most frequently mutated gene in human cancer, and over half of human cancers contain p53 mutations. Majority of p53 mutations in cancer are missense mutations, leading to the expression of full-length mutant p53 (mutp53) protein. While the crit...

journal_title：Journal of molecular biology

authors： Yue X,Zhao Y,Xu Y,Zheng M,Feng Z,Hu W

更新日期：2017-06-02 00:00:00

abstract：:Expression of the Caenorhabditis elegans cysteine protease gene cpr-1 is regulated both spatially and temporally. In situ hybridisation and Northern blot analysis have shown that this gene is expressed exclusively in gut cells of all developmental stages except the embryo. We now show by transgenic transformation with...

journal_title：Journal of molecular biology

authors： Britton C,McKerrow JH,Johnstone IL

更新日期：1998-01-01 00:00:00

abstract：:Chromatin remodeling is fundamental for the dynamical changes in transcriptional programs that occur during development and stem cell differentiation. The histone acetyltransferase Kat6b is relevant for neurogenesis in mouse embryos, and mutations of this gene cause intellectual disability in humans. However, the mole...

journal_title：Journal of molecular biology

authors： Cosentino MS,Oses C,Vázquez Echegaray C,Solari C,Waisman A,Álvarez Y,Petrone MV,Francia M,Schultz M,Sevlever G,Miriuka S,Levi V,Guberman A

更新日期：2019-03-15 00:00:00

abstract：:The CheY protein is the response regulator in bacterial chemotaxis. Phosphorylation of a conserved aspartyl residue induces structural changes that convert the protein from an inactive to an active state. The short half-life of the aspartyl-phosphate has precluded detailed structural analysis of the active protein. Pe...

journal_title：Journal of molecular biology

authors： Cho HS,Lee SY,Yan D,Pan X,Parkinson JS,Kustu S,Wemmer DE,Pelton JG

更新日期：2000-03-31 00:00:00

abstract：:Eukaryotic aminoacyl-tRNA synthetases are usually organized into high-molecular-weight complexes, the structure and function of which are poorly understood. We have previously described a yeast complex containing two aminoacyl-tRNA synthetases, methionyl-tRNA synthetase and glutamyl-tRNA synthetase, and one noncatalyt...

journal_title：Journal of molecular biology

authors： Karanasios E,Simader H,Panayotou G,Suck D,Simos G

更新日期：2007-12-07 00:00:00