Abstract：
:To address the mechanism of thermosensing and its implications for molecular engineering, we previously deconstructed the functional components of the bacterial thermosensor DesK, a histidine kinase with a five-span transmembrane domain that detects temperature changes. The system was first simplified by building a sensor that consists of a single chimerical transmembrane segment that retained full sensing capacity. Genetic and biophysical analysis of this minimal sensor enabled the identification of three modular components named determinants of thermodetection (DOTs). Here we combine and tune the DOTs to determine their contribution to activity. A transmembrane zipper represents the master DOT that drives a reversible and activating dimerization through the formation of hydrogen bonds. Our findings provide the mechanism and insights to construct a synthetic transmembrane helix based on a poly-valine scaffold that harbors the DOTs and regulates the activity. The construct constitutes a modular switch that may be exploited in biotechnology and genetic circuitry.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Inda ME,Vazquez DB,Fernández A,Cybulski LEdoi
10.1016/j.jmb.2019.01.025subject
Has Abstractpub_date
2019-03-01 00:00:00pages
1016-1024issue
5eissn
0022-2836issn
1089-8638pii
S0022-2836(18)30923-9journal_volume
431pub_type
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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