Control of gene expression in the P2-related template coliphages. III. DNA sequence of the major control region of phage 186.

abstract：:Gap junction channels are intercellular channels that form by docking the extracellular loops of connexin protein subunits. While the structure and function of gap junctions as intercellular channels have been characterized using different techniques, the physics of the inter-connexin interaction remain unknown. Moreo...

journal_title：Journal of molecular biology

authors： Rico F,Oshima A,Hinterdorfer P,Fujiyoshi Y,Scheuring S

更新日期：2011-09-09 00:00:00

abstract：:Structural analysis of delta131delta, a fragment model of the denatured state of staphylococcal nuclease, has been extended by obtaining long-range distance restraints between chain segments by paramagnetic relaxation enhancement. Fourteen unique PROXYL spin labels were introduced at sites that are solvent-exposed in ...

journal_title：Journal of molecular biology

authors： Gillespie JR,Shortle D

更新日期：1997-04-25 00:00:00

abstract：:Replacement of residues 228-230 or 228-232 of subdomain 4 in Dictyostelium actin with the corresponding Tetrahymena sequence (QTA to KAY replacement: half chimera-1; QTAAS to KAYKE replacement: full chimera) leads to a higher Ca(2+)-activation of the regulated acto-myosin subfragment-1 ATPase activity. The ratio of AT...

journal_title：Journal of molecular biology

authors： Matsuura Y,Stewart M,Kawamoto M,Kamiya N,Saeki K,Yasunaga T,Wakabayashi T

更新日期：2000-02-18 00:00:00

abstract：:Sequence-specific interactions between proteins and DNA are essential for a variety of biological functions. The (cytosine-C5)-methyltransferase from HhaI (M.HhaI) specifically modifies the second base in GCGC sequences, employing a base flipping mechanism to access the target base being chemically modified. The mecha...

journal_title：Journal of molecular biology

authors： Huang N,MacKerell AD Jr

更新日期：2005-01-14 00:00:00

abstract：:We have developed a computer model for the simulation of simultaneous SELEX against multiple targets. The model assumes equilibrium behavior for the formation of binary ligand:target complexes, and that there is no ligand:ligand or target:target interaction. Target concentrations, ligand concentrations, and affinity d...

journal_title：Journal of molecular biology

authors： Vant-Hull B,Payano-Baez A,Davis RH,Gold L

更新日期：1998-05-08 00:00:00

abstract：:Nudaurelia capensis omega virus is a T=4, icosahedral virus with a bipartite, positive-sense RNA genome. Expression of the coat protein gene in a baculovirus system was previously shown to result in the formation of procapsids when purified at pH 7.6. Procapsids are round, porous particles (480 A diameter) and have T=...

journal_title：Journal of molecular biology

authors： Tang J,Lee KK,Bothner B,Baker TS,Yeager M,Johnson JE

更新日期：2009-09-25 00:00:00

abstract：:Abasic lesions are common mutagenic or cytotoxic DNA damages. Ape1 is the major human apurinic/apyrimidinic (AP) endonuclease and initiates repair of abasic sites by catalyzing strand cleavage at the lesion. I show here that Ape1 single-stranded (ss) AP site incision activity prefers 0.5 mM or 2 mM MgCl(2) and low con...

journal_title：Journal of molecular biology

authors： Wilson DM 3rd

更新日期：2005-02-04 00:00:00

abstract：:Mutants of an archaeon Halobacterium halobium, resistant to the universal inhibitor of translation, pactamycin, were isolated. Pactamycin resistance correlated with the presence of mutations in the 16 S rRNA gene of H. halobium single rRNA operon. Three types of mutations were found in pactamycin resistant cells, A694...

journal_title：Journal of molecular biology

authors： Mankin AS

更新日期：1997-11-21 00:00:00

abstract：:In order to investigate the modes of DNA synthesis supported by the 66 and 51 kDa subunits of equine infectious anemia virus reverse transcriptase (EIAV RT), recombinant p66 polypeptides containing a modified ribonuclease H (RNase H) domain were purified and evaluated. Defined heteropolymeric template-primer combinati...

journal_title：Journal of molecular biology

authors： Rausch JW,Arts EJ,Wöhrl BM,Le Grice SF

更新日期：1996-04-05 00:00:00

abstract：:Hsp90 is a highly conserved molecular chaperone that remodels hundreds of client proteins, many involved in the progression of cancer and other diseases. It functions with the Hsp70 chaperone and numerous cochaperones. The bacterial Hsp90 functions with an Hsp70 chaperone, DnaK, but is independent of Hsp90 cochaperone...

journal_title：Journal of molecular biology

authors： Genest O,Hoskins JR,Kravats AN,Doyle SM,Wickner S

更新日期：2015-12-04 00:00:00

abstract：:The three-dimensional structures of engineered variants of Bacillus lentus subtilisin having increased enzymatic activity, K27R/N87S/V104Y/N123S/T274A (RSYSA) and N76D/N87S/S103A/V104I (DSAI), were determined by X-ray crystallography. In addition to identifying changes in atomic position we report a method that identi...

journal_title：Journal of molecular biology

authors： Graycar T,Knapp M,Ganshaw G,Dauberman J,Bott R

更新日期：1999-09-10 00:00:00

abstract：:Mechanisms of folding and misfolding of membrane proteins are of interest in cell biology. Recently, we have established single-molecule force spectroscopy to observe directly the stepwise folding of the Na+/H+ antiporter NhaA from Escherichia coli in vitro. Here, we improved this approach significantly to track the f...

journal_title：Journal of molecular biology

authors： Kedrov A,Janovjak H,Ziegler C,Kuhlbrandt W,Muller DJ

更新日期：2006-01-06 00:00:00

abstract：:With a rapidly growing pool of known tertiary structures, the importance of protein structure comparison parallels that of sequence alignment. We have developed a novel algorithm (DALI) for optimal pairwise alignment of protein structures. The three-dimensional co-ordinates of each protein are used to calculate residu...

journal_title：Journal of molecular biology

authors： Holm L,Sander C

更新日期：1993-09-05 00:00:00

abstract：:The formation of two-dimensional crystals of the membrane-bound enzyme microsomal glutathione transferase is sensitive to fractional changes in the lipid-to-protein ratio. Variation of this parameter results in crystal polymorphism. The projection structure of a p6 crystal form of the enzyme has been determined by the...

journal_title：Journal of molecular biology

authors： Schmidt-Krey I,Murata K,Hirai T,Mitsuoka K,Cheng Y,Morgenstern R,Fujiyoshi Y,Hebert H

更新日期：1999-04-30 00:00:00

abstract：:The extracellular matrix in tissues such as bone, tendon and cornea contains ordered, parallel arrays of collagen type I fibrils. Cells embedded in these matrices frequently co-align with the collagen fibrils, suggesting that ordered fibrils provide structural or signalling cues for cell polarization. To study mechani...

journal_title：Journal of molecular biology

authors： Friedrichs J,Taubenberger A,Franz CM,Muller DJ

更新日期：2007-09-21 00:00:00

abstract：:Nitrite reductase from Pseudomonas aeruginosa (EC 1.9.3.2), a redox enzyme synthesized by the bacterium grown in the presence of nitrate, is a soluble dimer of two identical subunits of 60 kDa, each containing one c and one d1 haem as prosthetic groups. A new crystal from of the Ps. aeruginosa nitrite reductase in the...

journal_title：Journal of molecular biology

authors： Tegoni M,Silvestrini MC,Lamzin VS,Brunori M,Cambillau C

更新日期：1994-10-21 00:00:00

abstract：:Antibodies and prorenin mutants have long been used to structurally characterize prorenin, the inactive proenzyme form of renin. They were designed on the basis of homology models built using other aspartyl protease proenzyme structures since no structure was available for prorenin. Here, we present the first X-ray st...

journal_title：Journal of molecular biology

authors： Morales R,Watier Y,Böcskei Z

更新日期：2012-08-03 00:00:00

abstract：:The gene archb encoding for the cell-bound chitobiase from the Antarctic Gram-positive bacterium Arthrobacter sp. TAD20 was cloned and expressed in Escherichia coli in a soluble form. The mature chitobiase ArChb possesses four functionally independent domains: a catalytic domain stabilized by Ca(2+), a galactose-bindi...

journal_title：Journal of molecular biology

authors： Lonhienne T,Zoidakis J,Vorgias CE,Feller G,Gerday C,Bouriotis V

更新日期：2001-07-06 00:00:00

abstract：:3-Methylitaconate-Delta-isomerase (Mii) participates in the nicotinate fermentation pathway of the anaerobic soil bacterium Eubacterium barkeri (order Clostridiales) by catalyzing the reversible conversion of (R)-3-methylitaconate (2-methylene-3-methylsuccinate) to 2,3-dimethylmaleate. The enzyme is also able to catal...

journal_title：Journal of molecular biology

authors： Velarde M,Macieira S,Hilberg M,Bröker G,Tu SM,Golding BT,Pierik AJ,Buckel W,Messerschmidt A

更新日期：2009-08-21 00:00:00

abstract：:The folding thermodynamics and kinetics of the alpha-spectrin SH3 domain with a redesigned hydrophobic core have been studied. The introduction of five replacements, A11V, V23L, M25V, V44I and V58L, resulted in an increase of 16% in the overall volume of the side-chains forming the hydrophobic core but caused no remar...

journal_title：Journal of molecular biology

authors： Cobos ES,Filimonov VV,Vega MC,Mateo PL,Serrano L,Martínez JC

更新日期：2003-04-18 00:00:00

abstract：:Isoniazid (INH) was the first synthesized drug that mediated bactericidal killing of the bacterium Mycobacterium tuberculosis, a major clinical breakthrough. To this day, INH remains a cornerstone of modern tuberculosis (TB) chemotherapy. This review describes the serendipitous discovery of INH, its effectiveness on T...

journal_title：Journal of molecular biology

authors： Vilchèze C,Jacobs WR Jr

更新日期：2019-08-23 00:00:00

abstract：:A 1.9 A resolution X-ray structure of the apo-form of Corynebacterium 2,5-diketo-d-gluconic acid reductase A (2,5-DKGR A), a member of the aldo-keto reductase superfamily, has been determined by molecular replacement using the NADPH-bound form of the same enzyme as the search model. 2,5-DKGR A catalyzes the NADPH-depe...

journal_title：Journal of molecular biology

authors： Sanli G,Blaber M

更新日期：2001-06-22 00:00:00

abstract：:Designed retroaldolases have utilized a nucleophilic lysine to promote carbon-carbon bond cleavage of β-hydroxy-ketones via a covalent Schiff base intermediate. Previous computational designs have incorporated a water molecule to facilitate formation and breakdown of the carbinolamine intermediate to give the Schiff b...

journal_title：Journal of molecular biology

authors： Bjelic S,Kipnis Y,Wang L,Pianowski Z,Vorobiev S,Su M,Seetharaman J,Xiao R,Kornhaber G,Hunt JF,Tong L,Hilvert D,Baker D

更新日期：2014-01-09 00:00:00

abstract：:Quantitatively describing RNA structure and conformational elements remains a formidable problem. Seven standard torsion angles and the sugar pucker are necessary to characterize the conformation of an RNA nucleotide completely. Progress has been made toward understanding the discrete nature of RNA structure, but clas...

journal_title：Journal of molecular biology

authors： Wadley LM,Keating KS,Duarte CM,Pyle AM

更新日期：2007-09-28 00:00:00

abstract：:The C repeat family was first observed in the rabbit beta-like gene family. We have estimated the repetition frequency of the C repeats, determined the nucleotide sequence of three intact members and one truncated member, and have investigated the size, tissue specificity, and intracellular localization of C repeat tr...

journal_title：Journal of molecular biology

authors： Cheng JF,Printz R,Callaghan T,Shuey D,Hardison RC

更新日期：1984-06-15 00:00:00

abstract：:ThiI is an enzyme responsible for the formation of the modified base S(4)U (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This base acts as a sensitive trigger for the response mechanism to UV exposure, providing protection against its damaging effects. We present the crystal structure of Bacillus anth...

journal_title：Journal of molecular biology

authors： Waterman DG,Ortiz-Lombardía M,Fogg MJ,Koonin EV,Antson AA

更新日期：2006-02-10 00:00:00

abstract：:Proton transfer through membrane-bound ion channels is mediated by both water and polar residues of proteins, but the detailed molecular mechanism is challenging to determine. The tetrameric influenza A and B virus M2 proteins form canonical proton channels that use an HxxxW motif for proton selectivity and gating. Th...

journal_title：Journal of molecular biology

authors： Kwon B,Roos M,Mandala VS,Shcherbakov AA,Hong M

更新日期：2019-06-28 00:00:00

abstract：:Eukaryotic aminoacyl-tRNA synthetases are usually organized into high-molecular-weight complexes, the structure and function of which are poorly understood. We have previously described a yeast complex containing two aminoacyl-tRNA synthetases, methionyl-tRNA synthetase and glutamyl-tRNA synthetase, and one noncatalyt...

journal_title：Journal of molecular biology

authors： Karanasios E,Simader H,Panayotou G,Suck D,Simos G

更新日期：2007-12-07 00:00:00

abstract：:Despite the fact that DNA polymerases have been investigated for many years and are commonly used as tools in a number of molecular biology assays, many details of the kinetic mechanism they use to catalyze DNA synthesis remain unclear. Structural and kinetic studies have characterized a rapid, pre-catalytic open-to-c...

journal_title：Journal of molecular biology

authors： Xu C,Maxwell BA,Suo Z

更新日期：2014-08-12 00:00:00

abstract：:We have sequenced the heavy and light chain genes from 365 IgG(+) B cells and found that 24 (6.5 %) contain somatically introduced insertions or deletions. These insertions and deletions are clustered at "hot-spots" in the antigen-binding site and frequently result in the creation of new combinations of canonical loop...

journal_title：Journal of molecular biology

authors： de Wildt RM,van Venrooij WJ,Winter G,Hoet RM,Tomlinson IM

更新日期：1999-12-03 00:00:00