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Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain.

Abstract：

:ThiI is an enzyme responsible for the formation of the modified base S(4)U (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This base acts as a sensitive trigger for the response mechanism to UV exposure, providing protection against its damaging effects. We present the crystal structure of Bacillus anthracis ThiI in complex with AMP, revealing an extended tripartite architecture in which an N-terminal ferredoxin-like domain (NFLD) connects the C-terminal catalytic PP-loop pyrophosphatase domain with a THUMP domain, an ancient predicted RNA-binding domain that is widespread in all kingdoms of life. We describe the structure of the THUMP domain, which appears to be unrelated to RNA-binding domains of known structure. Mapping the conserved residues of NFLD and the THUMP domain onto the ThiI structure suggests that these domains jointly form the tRNA-binding surface. The inaccessibility of U8 in the canonical L-shaped form of tRNA, and the existence of a glycine-rich linker joining the catalytic and RNA-binding moieties of ThiI suggest that structural changes may occur in both molecules upon binding.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Waterman DG,Ortiz-Lombardía M,Fogg MJ,Koonin EV,Antson AA

doi

10.1016/j.jmb.2005.11.013

keywords:

subject

Has Abstract

pub_date

2006-02-10 00:00:00

pages

97-110

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(05)01399-9

journal_volume

356

pub_type

杂志文章

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