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Static disorder and librational motions of the purine bases in films of oriented Li-DNA.

Abstract：

:Solid-state 2H nuclear magnetic resonance line shapes have been obtained from folded films of oriented Li-DNA molecules with the purine bases selectively labeled with deuterium at the 8-position. From line shape simulations, the static base tilts as well as the anisotropic motional amplitudes were determined as a function of hydration level and temperature. It was found that the average tilt angle of the bases is close to 0 degrees and at a hydration of ten water molecules per nucleotide the distribution width of tilt angles about this average cannot be larger than 9 degrees (standard deviation). A slightly increased distribution width is observed at low hydration levels. The motional amplitudes are hydration dependent, with the tilting motion ranging from 4 degrees for the driest, up to 15 degrees for the wettest sample, and slightly larger amplitudes are observed for the twisting motion. The amplitude of the twisting motion is unaffected by a temperature decrease down to -60 degrees C, in contrast to the tilting motion that is suppressed at low temperatures.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Brandes R,Vold RR,Kearns DR,Rupprecht A

doi

10.1016/0022-2836(88)90461-5

subject

Has Abstract

pub_date

1988-07-20 00:00:00

pages

321-32

issue

2

eissn

0022-2836

issn

1089-8638

pii

0022-2836(88)90461-5

journal_volume

202

pub_type

杂志文章

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