当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Crystallization of a new form of the eye lens protein beta B2-crystallin.

Crystallization of a new form of the eye lens protein beta B2-crystallin.

Abstract：

:A new crystal form of the bovine oligomeric lens protein beta B2 has been grown in the presence of calcium acetate. The crystals are orthorhombic, I222 or I2(1)2(1)2(1), with cell dimensions a = 77.8 A, b = 83.6 A, c = 109.2 A. This new crystal form, which diffracts to at least 2.5 A, has a and b cell dimensions that are half those of the original crystal form, although there is no simple relationship between the c cell dimensions. The new crystal form reported here contains only one subunit per asymmetric unit, indicating that the dimer lies on a crystallographic 2-fold axis, and is a suitable candidate for molecular replacement studies.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Bax B,Slingsby C

doi

10.1016/0022-2836(89)90162-9

subject

Has Abstract

pub_date

1989-08-20 00:00:00

pages

715-7

issue

4

eissn

0022-2836

issn

1089-8638

pii

0022-2836(89)90162-9

journal_volume

208

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • A ligand-centric analysis of the diversity and evolution of protein-ligand relationships in E.coli.

    abstract：:As enzymes evolve and diverge from common ancestor sequences, they often keep their overall reaction chemistry but specialize in the binding of different cognate ligands. This study borrows methods for the computational assessment of 2D similarity of small molecules from the field of chemoinformatics, to examine the e...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.01.061

    authors： Nobeli I,Spriggs RV,George RA,Thornton JM

    更新日期：2005-03-25 00:00:00

  • Topological and energetic factors: what determines the structural details of the transition state ensemble and "en-route" intermediates for protein folding? An investigation for small globular proteins.

    abstract：:Recent experimental results suggest that the native fold, or topology, plays a primary role in determining the structure of the transition state ensemble, at least for small, fast-folding proteins. To investigate the extent of the topological control of the folding process, we studied the folding of simplified models ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3693

    authors： Clementi C,Nymeyer H,Onuchic JN

    更新日期：2000-05-19 00:00:00

  • Crystal structure of the agrin-responsive immunoglobulin-like domains 1 and 2 of the receptor tyrosine kinase MuSK.

    abstract：:Muscle-specific kinase (MuSK) is a receptor tyrosine kinase expressed exclusively in skeletal muscle, where it is required for formation of the neuromuscular junction. MuSK is activated by agrin, a neuron-derived heparan sulfate proteoglycan. Here, we report the crystal structure of the agrin-responsive first and seco...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.09.019

    authors： Stiegler AL,Burden SJ,Hubbard SR

    更新日期：2006-12-01 00:00:00

  • Intimate view of a kinetic protein folding intermediate: residue-resolved structure, interactions, stability, folding and unfolding rates, homogeneity.

    abstract：:A cytochrome c kinetic folding intermediate was studied by hydrogen exchange (HX) pulse labeling. Advances in the technique and analysis made it possible to define the structured and unstructured regions, equilibrium stability, and kinetic opening and closing rates, all at an amino acid-resolved level. The entire N-te...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2003.09.070

    authors： Krishna MM,Lin Y,Mayne L,Englander SW

    更新日期：2003-11-28 00:00:00

  • Positive selection of synonymous mutations in vesicular stomatitis virus.

    abstract：:Prevailing evolutionary forces are typically deduced from the pattern of differences in synonymous and non-synonymous mutations, under the assumption of neutrality in the absence of amino acid change. We determined the complete sequence of ten vesicular stomatitis virus populations evolving under positive selection. A...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.08.003

    authors： Novella IS,Zárate S,Metzgar D,Ebendick-Corpus BE

    更新日期：2004-10-01 00:00:00

  • The RecG branch migration protein of Escherichia coli dissociates R-loops.

    abstract：:The RuvAB and RecG proteins of Escherichia coli promote branch migration of Holliday junction intermediates in genetic recombination. Both are structure-specific helicases that unwind and rewind DNA at the junction point. The helicase activities of these proteins were investigated using RNA:DNA hybrid molecules. RuvAB...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0671

    authors： Vincent SD,Mahdi AA,Lloyd RG

    更新日期：1996-12-13 00:00:00

  • Cloning and characterization of a novel Bacillus thuringiensis cytolytic delta-endotoxin.

    abstract：:A gene encoding a major entomocidal polypeptide from Bacillus thuringiensis subspecies kyushuensis delta-endotoxin crystals (CytB) was cloned into Escherichia coli and sequenced. The deduced amino acid sequence gave a predicted molecular mass of 29,236 Da and showed 39% identity and 70% similarity with the 27,371 Da C...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1037

    authors： Koni PA,Ellar DJ

    更新日期：1993-01-20 00:00:00

  • Cooperative effects of cofilin (ADF) on actin structure suggest allosteric mechanism of cofilin function.

    abstract：:Using site-specific fluorescence probes and cross-linking we demonstrated that cofilin (ADF), a key regulator of actin cellular dynamics, weakens longitudinal contacts in F-actin in a cooperative manner. Differential scanning calorimetry detected a dual nature of cofilin effects on F-actin conformation. At sub-stoichi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.11.072

    authors： Bobkov AA,Muhlrad A,Pavlov DA,Kokabi K,Yilmaz A,Reisler E

    更新日期：2006-02-17 00:00:00

  • A conformational transition at the N terminus of the prion protein features in formation of the scrapie isoform.

    abstract：:The scrapie prion protein (PrPSc) is formed from the cellular isoform (PrPC) by a post-translational process that involves a profound conformational change. Linear epitopes for recombinant antibody Fab fragments (Fabs) on PrPC and on the protease-resistant core of PrPSc, designated PrP 27-30, were identified using ELI...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1328

    authors： Peretz D,Williamson RA,Matsunaga Y,Serban H,Pinilla C,Bastidas RB,Rozenshteyn R,James TL,Houghten RA,Cohen FE,Prusiner SB,Burton DR

    更新日期：1997-10-31 00:00:00

  • Crystal structure of the C-terminal domain of S.cerevisiae eIF5.

    abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.03.037

    authors： Wei Z,Xue Y,Xu H,Gong W

    更新日期：2006-05-26 00:00:00

  • Dissecting the Conformational Dynamics of the Bile Acid Transporter Homologue ASBTNM.

    abstract：:Apical sodium-dependent bile acid transporter (ASBT) catalyses uphill transport of bile acids using the electrochemical gradient of Na+ as the driving force. The crystal structures of two bacterial homologues ASBTNM and ASBTYf have previously been determined, with the former showing an inward-facing conformation, and ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.166764

    authors： Lu PH,Li CC,Chiang YW,Liu JH,Chiang WT,Chao YH,Li GS,Weng SE,Lin SY,Hu NJ

    更新日期：2021-01-13 00:00:00

  • Molecular phylogeny of the alphaherpesvirinae subfamily and a proposed evolutionary timescale.

    abstract：:Phylogenetic trees were derived for the Alphaherpesvirinae subfamily of the Herpesviridae using molecular sequences. Sequences from the families of genes encoding glycoprotein B, thymidine kinase, S region protein kinase, immediate-early transcriptional regulator IE175 and ribonucleotide reductase large subunit were e...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1264

    authors： McGeoch DJ,Cook S

    更新日期：1994-04-22 00:00:00

  • How thioredoxin can reduce a buried disulphide bond.

    abstract：:We present a study of the interaction between thioredoxin and the model enzyme pI258 arsenate reductase (ArsC) from Staphylococcus aureus. ArsC catalyses the reduction of arsenate to arsenite. Three redox active cysteine residues (Cys10, Cys82 and Cys89) are involved. After a single catalytic arsenate reduction event,...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.04.016

    authors： Messens J,Van Molle I,Vanhaesebrouck P,Limbourg M,Van Belle K,Wahni K,Martins JC,Loris R,Wyns L

    更新日期：2004-06-04 00:00:00

  • On the connection between inherent DNA flexure and preferred binding of hydroxymethyluracil-containing DNA by the type II DNA-binding protein TF1.

    abstract：:TF1 is a member of the family of type II DNA-binding proteins, which also includes the bacterial HU proteins and the Escherichia coli integration host factor (IHF). Distinctive to TF1, which is encoded by the Bacillus subtilis bacteriophage SPO1, is its preferential binding to DNA in which thymine is replaced by 5-hyd...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0392

    authors： Grove A,Galeone A,Mayol L,Geiduschek EP

    更新日期：1996-07-12 00:00:00

  • A large region controls tRNA gene transcription.

    abstract：:We find that tRNA gene transcription is controlled by a region much larger than the previously described internal segments known as A and B boxes. In a Bombyx mori silkworm tRNA2Ala gene, transcriptionally important sequences extend at least from -13 to +146, and thus include sequences both upstream and downstream fro...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(85)90209-8

    authors： Wilson ET,Larson D,Young LS,Sprague KU

    更新日期：1985-05-25 00:00:00

  • Dr. Jekyll and Mr. Hide: How Enterococcus faecalis Subverts the Host Immune Response to Cause Infection.

    abstract：:Enterococcus faecalis, a ubiquitous member of the healthy human gut microbiota, is also a common opportunistic pathogen and leading cause of nosocomial infections. This tenacious microbe is well adapted to infect and persist in multiple niches within the mammalian host and can rapidly tune its metabolism to respond to...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2019.05.030

    authors： Kao PHN,Kline KA

    更新日期：2019-07-26 00:00:00

  • Interaction of the Hsp110 molecular chaperones from S. cerevisiae with substrate protein.

    abstract：:Hsp110 proteins act as nucleotide exchange factors of the molecular chaperone Hsp70 in eukaryotes. In addition, they have been reported to stabilize unfolded proteins for subsequent refolding. Hsp110 proteins belong to the Hsp70 superfamily and, in analogy to Hsp70, the substrate-binding site was proposed to be locate...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.07.004

    authors： Polier S,Hartl FU,Bracher A

    更新日期：2010-09-03 00:00:00

  • Crystallization of a chymotrypsin inhibitor from Erythrina caffra seeds.

    abstract：:Crystals of a chymotrypsin inhibitor from Erythrina caffra seeds have been grown out of lithium sulfate, by the hanging drop method of vapor diffusion. The crystals belong to the rhombohedral space group R32, with a = 67.2 A and alpha = 99.4 degrees, and diffract to 3 A resolution. ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(90)90300-B

    authors： Shieh HS,Leimgruber NK,Palmier MO,Wun TC,Leimgruber RM,Abdel-Meguid SS

    更新日期：1990-03-05 00:00:00

  • Identifying functionally important conformational changes in proteins: activation of the yeast α-factor receptor Ste2p.

    abstract：:We have developed a procedure in which disulfide cross-links are used to identify regions of proteins that undergo functionally important intramolecular motion. The approach was applied to the identification of disulfide bonds that stabilize the active state of the yeast α-mating pheromone receptor Ste2p, a member of ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.02.024

    authors： Taslimi A,Mathew E,Celić A,Wessel S,Dumont ME

    更新日期：2012-05-18 00:00:00

  • Crystallization of murine major histocompatibility complex class I H-2Kb with single peptides.

    abstract：:X-ray quality crystals of a soluble murine class I H-2Kb molecule complexed with three different peptide antigens were grown in several forms by streak seeding and macroseeding methods. Co-crystals with VSV-8 (RGYVYGQL), OVA-8 (SIINFEKL) and SEV-9 (FAPGNYPAL) peptides were grown either from NaH2PO4/HPO4 or from polyet...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90881-j

    authors： Stura EA,Matsumura M,Fremont DH,Saito Y,Peterson PA,Wilson IA

    更新日期：1992-12-05 00:00:00

  • Structural bases for sulfide recognition in Lucina pectinata hemoglobin I.

    abstract：:The X-ray crystal structure of the sulfide derivative of ferric Lucina pectinata hemoglobin component I (HbI) has been determined at 1.9 A resolution (R-factor 0.186). The heme pocket structural organization of HbI is in keeping with its ligand binding properties. The fast sulfide association rate constant can be rela...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0228

    authors： Rizzi M,Wittenberg JB,Coda A,Ascenzi P,Bolognesi M

    更新日期：1996-04-26 00:00:00

  • Lipid molecules induce p38α activation via a novel molecular switch.

    abstract：:p38α mitogen-activated protein kinase (MAPK) is generally activated by dual phosphorylation but has also been shown to exhibit alternative activation modes. One of these modes included a direct interaction with phosphatidylinositol ether lipid analogues (PIA) inducing p38α autoactivation and apoptosis. Perifosine, an ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.10.007

    authors： Tzarum N,Eisenberg-Domovich Y,Gills JJ,Dennis PA,Livnah O

    更新日期：2012-12-14 00:00:00

  • Structure of nucleosomal DNA at high salt concentration as probed by hydroxyl radical.

    abstract：:The structure of a 146 base-pair nucleosomal DNA has been probed using hydroxyl radical cleavage in buffers containing NaCl concentrations ranging from 80 mM to 800 mM. The highest salt concentrations used here are close to those required to dissociate core histone H2A and H2B from nucleosomal DNA. Nonetheless, the cl...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1089

    authors： Puhl HL,Behe MJ

    更新日期：1993-02-20 00:00:00

  • Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli.

    abstract：:Two-component regulatory systems allow cells to adapt to environmental changes. In Escherichia coli, the TorS/TorR two-component system induces the expression of the tor structural operon encoding the trimethylamine N-oxide reductase respiratory system in response to substrate availability. TorS belongs to a sensor su...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.0919

    authors： Jourlin C,Ansaldi M,Méjean V

    更新日期：1997-04-11 00:00:00

  • The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach.

    abstract：:A protein microarray system containing different dilutions of 77 related and non-related proteins was used to show that IgE from subjects allergic to Brazil nut specifically recognise the seed 2S albumin protein (Ber e 1). Further, correctly folded chimaeric 2S albumin proteins containing structural epitope replacemen...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.08.065

    authors： Alcocer MJ,Murtagh GJ,Wilson PB,Progias P,Lin J,Archer DB

    更新日期：2004-10-22 00:00:00

  • Capsids and Portals Influence Each Other's Conformation During Assembly and Maturation.

    abstract：:The portal proteins of tailed bacteriophage and Herpesvirus capsids form dodecameric rings that occupy one capsid vertex and are incorporated during the assembly of capsid precursors called procapsids or proheads. Portals are essential and serve as the pore for DNA transit and the site of tail attachment; however, bac...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.01.022

    authors： Maurer JB,Oh B,Moyer CL,Duda RL

    更新日期：2020-03-27 00:00:00

  • The nature of ligand-induced conformational change in transferrin in solution. An investigation using X-ray scattering, XAFS and site-directed mutants.

    abstract：:Ligand-induced conformational change in transferrins has been studied by site-directed mutagenesis of human serum half molecule (N-lobe), X-ray absorption fine structure (XAFS) spectroscopy and X-ray solution scattering. Use of recent advances in data analysis has been made for extracting model-independent molecular s...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.1787

    authors： Grossmann JG,Crawley JB,Strange RW,Patel KJ,Murphy LM,Neu M,Evans RW,Hasnain SS

    更新日期：1998-06-05 00:00:00

  • Relative Binding Affinity Prediction of Charge-Changing Sequence Mutations with FEP in Protein-Protein Interfaces.

    abstract：:Building on the substantial progress that has been made in using free energy perturbation (FEP) methods to predict the relative binding affinities of small molecule ligands to proteins, we have previously shown that results of similar quality can be obtained in predicting the effect of mutations on the binding affinit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.02.003

    authors： Clark AJ,Negron C,Hauser K,Sun M,Wang L,Abel R,Friesner RA

    更新日期：2019-03-29 00:00:00

  • pK values of histidine residues in ribonuclease Sa: effect of salt and net charge.

    abstract：:The primary goal of this study was to gain a better understanding of the effect of environment and ionic strength on the pK values of histidine residues in proteins. The salt-dependence of pK values for two histidine residues in ribonuclease Sa (RNase Sa) (pI=3.5) and a variant in which five acidic amino acids have be...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01274-3

    authors： Huyghues-Despointes BM,Thurlkill RL,Daily MD,Schell D,Briggs JM,Antosiewicz JM,Pace CN,Scholtz JM

    更新日期：2003-01-31 00:00:00

  • Structural Insights into Substrate Recognition and Activity Regulation of the Key Decarboxylase SbnH in Staphyloferrin B Biosynthesis.

    abstract：:Staphyloferrin B is a hydroxycarboxylate siderophore that is crucial for the invasion and virulence of Staphylococcus aureus in mammalian hosts where free iron ions are scarce. The assembly of staphyloferrin B involves four enzymatic steps, in which SbnH, a pyridoxal 5'-phosphate (PLP)-dependent decarboxylase, catalyz...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.10.009

    authors： Tang J,Ju Y,Gu Q,Xu J,Zhou H

    更新日期：2019-12-06 00:00:00