A conformational transition at the N terminus of the prion protein features in formation of the scrapie isoform.

abstract：:The dominant assumption central to most treatments for sickle cell anemia has been that replacement of sickle hemoglobin (HbS) by fetal hemoglobin (HbF) would have major clinical benefit. Using laser photolysis, we have measured polymerization kinetics including rates of homogeneous and heterogeneous nucleation on mix...

journal_title：Journal of molecular biology

authors： Rotter M,Aprelev A,Adachi K,Ferrone FA

更新日期：2005-04-15 00:00:00

abstract：:Random sequencing of cDNA and genomic libraries has been used to study the genome of the hyperthermophile Thermotoga maritima. To date, 175 unique clones have been analyzed by comparing short sequence tags with known proteins in the PIR and GenBank databases. We find that a significant proportion of sequences can be m...

journal_title：Journal of molecular biology

authors： Kim CW,Markiewicz P,Lee JJ,Schierle CF,Miller JH

更新日期：1993-06-20 00:00:00

abstract：:The four approximately 75-residue domains (repeats) that constitute the annexin core structure all possess an identical five-alpha-helix bundle topology, but the physico-chemical properties of the isolated domains are different. Domain IV of the annexins has previously been expressed only as inclusion bodies, resistan...

journal_title：Journal of molecular biology

authors： Aukrust I,Evensen L,Hollås H,Berven F,Atkinson RA,Travé G,Flatmark T,Vedeler A

更新日期：2006-10-20 00:00:00

abstract：:The MotA protein of Escherichia coli is a component of the flagellum that functions, together with MotB, in transmembrane proton conduction. MotA and MotB are believed to form the stator of the flagellar motor. They are integral membrane proteins; MotA has a large (ca 22 kDa) domain in the cytoplasm, and MotB a much s...

journal_title：Journal of molecular biology

authors： Zhou J,Blair DF

更新日期：1997-10-24 00:00:00

abstract：:Current techniques for the prediction of side-chain conformations on a fixed backbone have an accuracy limit of about 1.0-1.5 A rmsd for core residues. We have carried out a detailed and systematic analysis of the factors that influence the prediction of side-chain conformation and, on this basis, have succeeded in ex...

journal_title：Journal of molecular biology

authors： Xiang Z,Honig B

更新日期：2001-08-10 00:00:00

abstract：:Collagen fibrils are the principle source of mechanical strength in the mutable dermis of the sea cucumber Cucumaria frondosa. To obtain information about the mechanism by which collagen molecules self-assemble into fibrils, we have isolated single intact fibrils with lengths in the range 14-444 microm. These fibrils ...

journal_title：Journal of molecular biology

authors： Trotter JA,Chapman JA,Kadler KE,Holmes DF

更新日期：1998-12-18 00:00:00

abstract：:Heterodimeric transcription regulatory proteins that bind palindromic DNA sequences can potentially bind their recognition sites in two opposite orientations. The orientation of transcription factor binding can control transcriptional activity by altering interactions with proteins that bind to adjacent regulatory ele...

journal_title：Journal of molecular biology

authors： Ramirez-Carrozzi VR,Kerppola TK

更新日期：2001-01-19 00:00:00

abstract：:Crystals of seryl-tRNA synthetase from Escherichia coli can be grown from ammonium sulphate/octyl glucoside solutions in two days. The crystals appear to be very suitable for X-ray analysis, diffracting to at least 2.8 A resolution and being resistant to radiation damage. The crystals are monoclinic (space group C2) w...

journal_title：Journal of molecular biology

authors： Leberman R,Berthet-Colominas C,Cusack S,Härtlein M

更新日期：1987-01-20 00:00:00

abstract：:IL-22 is a class 2 alpha-helical cytokine involved in the generation of inflammatory responses. These activities require IL-22 to engage the cell surface receptors IL-22R1 and the low-affinity signaling molecule IL-10R2. IL-10R2 also interacts with five other class 2 cytokines: IL-10, IL-26, and the interferon-like cy...

journal_title：Journal of molecular biology

authors： Logsdon NJ,Jones BC,Allman JC,Izotova L,Schwartz B,Pestka S,Walter MR

更新日期：2004-09-10 00:00:00

abstract：:Proteins containing the STAR RNA-binding domain fulfill vital roles in RNA biogenesis, yet a detailed understanding of STAR domain RNA binding specificity is lacking. In Caenorhabditis elegans, the STAR protein GLD-1 directly binds the 28 nucleotide recognition element TGE within the 3' untranslated region of tra-2 mR...

journal_title：Journal of molecular biology

authors： Lehmann-Blount KA,Williamson JR

更新日期：2005-02-11 00:00:00

abstract：:The majority of mitochondrial proteins can be imported into mitochondria following termination of their translation in the cytosol. Import of fumarase and several other proteins into mitochondria does not appear to occur post-translationally according to standard in vivo and in vitro assays. However, the nature of int...

journal_title：Journal of molecular biology

authors： Karniely S,Regev-Rudzki N,Pines O

更新日期：2006-04-28 00:00:00

abstract：:Rabbit liver aldolase was purified by affinity elution on a CM52 ion exchanger. Crystals of rabbit liver aldolase suitable for X-ray diffraction experiments were grown from 45% saturated ammonium sulfate solution at 4 degrees C. The enzyme crystallizes in space group C222(1) having cell dimensions a = 377.02 A, b = 13...

journal_title：Journal of molecular biology

authors： Sygusch J,Beaudry D

更新日期：1985-11-05 00:00:00

abstract：:An accurate three-dimensional structure is known for papain (1.65 A resolution) and actinidin (1.7 A). A detailed comparison of these two structures was performed to determine the effect of amino acid changes on the conformation. It appeared that, despite only 48% identity in their amino acid sequence, different cryst...

journal_title：Journal of molecular biology

authors： Kamphuis IG,Drenth J,Baker EN

更新日期：1985-03-20 00:00:00

abstract：:Native proteins exhibit precise geometric packing of atoms in their hydrophobic interiors. Nonetheless, controversy remains about the role of core side-chain packing in specifying and stabilizing the folded structures of proteins. Here we investigate the role of core packing in determining the conformation and stabili...

journal_title：Journal of molecular biology

authors： Liu J,Cao W,Lu M

更新日期：2002-05-03 00:00:00

abstract：:The folding/unfolding pathway of barnase has been studied extensively using the protein engineering method, which has provided indirect structural information for the transition state and the major folding intermediate. To further characterize the structural properties of the intermediate, we have simulated the therma...

journal_title：Journal of molecular biology

authors： Li A,Daggett V

更新日期：1998-01-30 00:00:00

abstract：:Exotoxin A of Pseudomonas aeruginosa asserts its cellular toxicity through ADP-ribosylation of translation elongation factor 2, predicated on binding to specific cell surface receptors and intracellular trafficking via a complex pathway that ultimately results in translocation of an enzymatic activity into the cytopla...

journal_title：Journal of molecular biology

authors： Wedekind JE,Trame CB,Dorywalska M,Koehl P,Raschke TM,McKee M,FitzGerald D,Collier RJ,McKay DB

更新日期：2001-12-07 00:00:00

abstract：:The role of electrostatic interactions in the stability and the folding of the N-terminal domain of the ribosomal protein L9 (NTL9) was investigated by determining the effects of varying the pH conditions. Urea denaturations and thermal unfolding experiments were used to measure the free energy of folding, DeltaG degr...

journal_title：Journal of molecular biology

authors： Luisi DL,Raleigh DP

更新日期：2000-06-16 00:00:00

abstract：:Packaging of viral genomes inside empty procapsids is driven by a powerful ATP-hydrolyzing motor, formed in many double-stranded DNA viruses by a complex of a small terminase (S-terminase) subunit and a large terminase (L-terminase) subunit, transiently docked at the portal vertex during genome packaging. Despite rece...

journal_title：Journal of molecular biology

authors： McNulty R,Lokareddy RK,Roy A,Yang Y,Lander GC,Heck AJR,Johnson JE,Cingolani G

更新日期：2015-10-09 00:00:00

abstract：:We produced human leukotriene B(4) (LTB(4)) receptor BLT1 as a recombinant protein in Escherichia coli. This detergent-solubilized receptor displays two states with regard to its affinity for LTB(4): (i) a low-affinity state (K(a)=7.8x10(8)M(-1)) that involves a receptor homodimer (BLT1.LTB(4))(2); we report evidence ...

journal_title：Journal of molecular biology

authors： Banères JL,Parello J

更新日期：2003-06-13 00:00:00

abstract：:A major puzzle is: are all glycoproteins routed through the ER calnexin pathway irrespective of whether this is required for their correct folding? Calnexin recognizes the terminal Glcalpha1-3Manalpha linkage, formed by trimming of the Glcalpha1-2Glcalpha1-3Glcalpha1-3Manalpha (Glc3Man) unit in Glc3Man9GlcNAc2. Differ...

journal_title：Journal of molecular biology

authors： Mackeen MM,Almond A,Deschamps M,Cumpstey I,Fairbanks AJ,Tsang C,Rudd PM,Butters TD,Dwek RA,Wormald MR

更新日期：2009-03-27 00:00:00

abstract：:Viruses must remain infectious while in harsh extracellular environments. An important aspect of viral particle stability for double-stranded DNA viruses is the energetically unfavorable state of the tightly confined DNA chain within the virus capsid creating pressures of tens of atmospheres. Here, we study the influe...

journal_title：Journal of molecular biology

authors： Bauer DW,Evilevitch A

更新日期：2015-10-09 00:00:00

abstract：:Using the binding of a nucleotide inhibitor (guanosine-3'-monophosphate) to a ribonuclease (ribonuclease Sa) as a model system, we show that the salt-dependence of the interaction arises due to specific ion binding at the site of nucleotide binding. The presence of specific ion-protein binding is concluded from a comb...

journal_title：Journal of molecular biology

authors： Waldron TT,Schrift GL,Murphy KP

更新日期：2005-02-25 00:00:00

abstract：:Here, we report a new computational method, called sheetminer, for mining beta-sheets in the density maps at intermediate resolutions of 6 to 10A. The method employs a multi-step ad hoc morphological analysis of density maps to identify the unique characteristics of beta-sheets. It was tested on density maps from 12 p...

journal_title：Journal of molecular biology

authors： Kong Y,Ma J

更新日期：2003-09-12 00:00:00

abstract：:The C repeat family was first observed in the rabbit beta-like gene family. We have estimated the repetition frequency of the C repeats, determined the nucleotide sequence of three intact members and one truncated member, and have investigated the size, tissue specificity, and intracellular localization of C repeat tr...

journal_title：Journal of molecular biology

authors： Cheng JF,Printz R,Callaghan T,Shuey D,Hardison RC

更新日期：1984-06-15 00:00:00

abstract：:The structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the trypanosomatid parasite Leishmania mexicana in a new crystal form has been determined by X-ray crystallography. The protein crystallizes in space group P21 with one 156 kDa tetramer per asymmetric unit. The model of the protein with ...

journal_title：Journal of molecular biology

authors： Kim H,Hol WG

更新日期：1998-04-24 00:00:00

abstract：:We report the first protein phase-diagram characterized by a combination of volumetric, calorimetric, and spectroscopic techniques. More specifically, we use ultrasonic velocimetry, densimetry, and differential scanning calorimetry, in conjunction with UV absorbance and CD spectroscopy to detect and to characterize th...

journal_title：Journal of molecular biology

authors： Chalikian TV,Völker J,Anafi D,Breslauer KJ

更新日期：1997-11-28 00:00:00

abstract：:The azole-based P450 inhibitor ketoconazole is used to treat fungal infections and functions by blocking ergosterol biosynthesis in yeast. Ketoconazole binds to mammalian P450 enzymes and this can result in drug-drug interactions and lead to liver damage. To identify protein-drug interactions that contribute to bindin...

journal_title：Journal of molecular biology

authors： Cupp-Vickery JR,Garcia C,Hofacre A,McGee-Estrada K

更新日期：2001-08-03 00:00:00

abstract：:The salivary gland cells in the dipteran Chironomus tentans produce approximately 15 different secretory proteins, with relative molecular masses ranging between 1 x 10(4) and 1 x 10(6). Together these proteins form two types of extra corporal tubes, a larval protective housing and feeding tube or a pupation tube. The...

journal_title：Journal of molecular biology

authors： Galli J,Wieslander L

更新日期：1993-05-20 00:00:00

abstract：:Recently, the absence of a thermally induced transition has been offered as proof that the unfolded and the molten globule states of apo-alpha-lactalbumin are enthalpically equivalent. In this paper we demonstrate that that argument is thermodynamically incorrect. In addition, it is shown that the absence of a thermal...

journal_title：Journal of molecular biology

authors： Xie D,Bhakuni V,Freire E

更新日期：1993-07-05 00:00:00

abstract：:Autophagosomes are double membrane organelles that are formed during a process referred to as macroautophagy. They serve to deliver cytoplasmic material into the lysosome for degradation. Autophagosomes are formed in a de novo manner and are the result of substantial membrane remodeling processes involving numerous pr...

journal_title：Journal of molecular biology

authors： Martens S,Nakamura S,Yoshimori T

更新日期：2016-10-27 00:00:00