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Crystals of seryl-tRNA synthetase from Escherichia coli. Preliminary crystallographic data.

Abstract：

:Crystals of seryl-tRNA synthetase from Escherichia coli can be grown from ammonium sulphate/octyl glucoside solutions in two days. The crystals appear to be very suitable for X-ray analysis, diffracting to at least 2.8 A resolution and being resistant to radiation damage. The crystals are monoclinic (space group C2) with cell parameters a = 148.2 A, b = 90.6 A, c = 69.5 A and beta = 119.0 degrees. Depending on whether the asymmetric unit is the enzyme monomer (Mr 48,414) or dimer the Vm value would be either 4.12 or 2.10 A3/dalton. Although the former would indicate a rather high solvent content, other proteins crystallized in the presence of octyl glucoside have Vm values similar to this.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Leberman R,Berthet-Colominas C,Cusack S,Härtlein M

doi

10.1016/0022-2836(87)90232-4

subject

Has Abstract

pub_date

1987-01-20 00:00:00

pages

423-5

issue

2

eissn

0022-2836

issn

1089-8638

pii

0022-2836(87)90232-4

journal_volume

193

pub_type

杂志文章

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