Crystallization and preliminary X-ray data of a phleomycin-binding protein from Streptoalloteichus hindustanus.

abstract：:The interaction sites of rabbit skeletal troponin I (TnI) with troponin C (TnC), troponin T (TnT), tropomyosin (Tm) and actin were mapped systematically using nine single cysteine residue TnI mutants with mutation sites at positions 6, 48, 64, 89, 104, 121, 133, 155 or 179 (TnI6, TnI48 etc.). Each mutant was labeled w...

journal_title：Journal of molecular biology

authors： Luo Y,Wu JL,Li B,Langsetmo K,Gergely J,Tao T

更新日期：2000-02-25 00:00:00

abstract：:A total of 76 independent spontaneous mutants in the hprt gene of V79 Chinese hamster cells have been analyzed. These mutants were obtained in two different laboratories, 17 and 59 mutants in sets 1 and 2, respectively, under different cell culture conditions. Mutation analysis was performed by amplification of hprt c...

journal_title：Journal of molecular biology

authors： Zhang LH,Vrieling H,van Zeeland AA,Jenssen D

更新日期：1992-02-05 00:00:00

abstract：:Pathogenic strains of Helicobacter pylori use a type IV secretion system (T4SS) to deliver the toxin CagA into human host cells. The T4SS, along with the toxin itself, is coded into a genomic insert, which is termed the cag pathogenicity island. The cag pathogenicity island contains about 30 open-reading frames, for m...

journal_title：Journal of molecular biology

authors： Cendron L,Couturier M,Angelini A,Barison N,Stein M,Zanotti G

更新日期：2009-02-13 00:00:00

abstract：:The initiator of coliphage lambda DNA replication, lambda O protein, may be detected among other 35S-labeled phage and bacterial proteins by a method based on immunoprecipitation. This method makes it possible to study lambda O proteolytic degradation in lambda plasmid-harboring or lambda phage-infected cells; it avoi...

journal_title：Journal of molecular biology

authors： Wegrzyn G,Pawlowicz A,Taylor K

更新日期：1992-08-05 00:00:00

abstract：:Biochemical functions of proteins in cells frequently involve interactions with various ligands. Proteomic methods for the identification of proteins that interact with specific ligands such as metabolites, signaling molecules, and drugs are valuable in investigating the regulatory mechanisms of cellular metabolism, a...

journal_title：Journal of molecular biology

authors： Liu PF,Kihara D,Park C

更新日期：2011-04-22 00:00:00

abstract：:The basic leucine zipper domain of the yeast transcription factor GCN4 consists of a C-terminal leucine zipper and an N-terminal basic DNA-binding region that achieves a stable structure only after association with DNA. Backbone dynamics of a peptide encompassing the basic and leucine zipper bZip domain (residues 226-...

journal_title：Journal of molecular biology

authors： Bracken C,Carr PA,Cavanagh J,Palmer AG 3rd

更新日期：1999-02-05 00:00:00

abstract：:UMP kinase (UMPK), the enzyme responsible for microbial UMP phosphorylation, plays a key role in pyrimidine nucleotide biosynthesis, regulating this process via feed-back control and via gene repression of carbamoyl phosphate synthetase (the first enzyme of the pyrimidine biosynthesis pathway). We present crystal stru...

journal_title：Journal of molecular biology

authors： Marco-Marín C,Gil-Ortiz F,Rubio V

更新日期：2005-09-16 00:00:00

abstract：:l-Rhamnose is a 6-deoxyhexose that is found in a variety of different glycoconjugates in the cell walls of pathogenic bacteria. The precursor of l-rhamnose is dTDP-l-rhamnose, which is synthesised from glucose- 1-phosphate and deoxythymidine triphosphate (dTTP) via a pathway requiring four enzymes. Significantly this ...

journal_title：Journal of molecular biology

authors： Allard ST,Giraud MF,Whitfield C,Graninger M,Messner P,Naismith JH

更新日期：2001-03-16 00:00:00

abstract：:Infection of Escherichia coli by filamentous bacteriophages is mediated by the minor phage coat protein g3p and involves two distinct cellular receptors, the F' pilus and the periplasmic protein TolA. Recently we have shown that the two receptors are contacted in a sequential manner, such that binding of TolA by the N...

journal_title：Journal of molecular biology

authors： Holliger P,Riechmann L,Williams RL

更新日期：1999-05-14 00:00:00

abstract：:Scaffold or matrix-attachment regions (S/MARs) are thought to be involved in the organization of eukaryotic chromosomes and in the regulation of several DNA functions. Their characteristics are conserved between plants and humans, and a variety of biological activities have been associated with them. The identificatio...

journal_title：Journal of molecular biology

authors： Bode J,Winkelmann S,Götze S,Spiker S,Tsutsui K,Bi C,A K P,Benham C

更新日期：2006-04-28 00:00:00

abstract：:Three photosynthetic complexes, light-harvesting complex 2 (LH2), light-harvesting complex 1 (LH1), and the reaction centre-light-harvesting complex 1 photounit (RC-LH1), were purified from a single species of a purple bacterium, Rhodobacter sphaeroides, and reconstituted into two-dimensional (2-D) crystals. Vesicular...

journal_title：Journal of molecular biology

authors： Walz T,Jamieson SJ,Bowers CM,Bullough PA,Hunter CN

更新日期：1998-10-02 00:00:00

abstract：:DNA replication fork arrest during the termination phase of chromosome replication in Bacillus subtilis is brought about by the replication terminator protein (RTP) bound to specific DNA terminator sequences (Ter sites) distributed throughout the terminus region. An attractive suggestion by others was that crucial to ...

journal_title：Journal of molecular biology

authors： Duggin IG,Andersen PA,Smith MT,Wilce JA,King GF,Wake RG

更新日期：1999-03-12 00:00:00

abstract：:The protein composition of the various muscle types in Drosophila melanogaster has been studied quite thoroughly and the analysis has revealed many differences involving the usage of muscle specific isoforms of a given protein, as well as the presence of proteins restricted to one muscle type. Drosophila projectin, th...

journal_title：Journal of molecular biology

authors： Daley J,Southgate R,Ayme-Southgate A

更新日期：1998-05-29 00:00:00

abstract：:UDP-glucose is the universal activated form of glucose, employed in all organisms for glucosyl transfer reactions and as precursor for various activated carbohydrates. In animal and fungal metabolism, UDP-glucose is required for utilization of galactose and for the synthesis of glycogen, the major carbohydrate storage...

journal_title：Journal of molecular biology

authors： Roeben A,Plitzko JM,Körner R,Böttcher UM,Siegers K,Hayer-Hartl M,Bracher A

更新日期：2006-12-08 00:00:00

abstract：:Mechanisms by which many N-terminal propeptides facilitate folding of proteins are unknown. The maturation of such proteins from their precursors involve three steps, namely: (1) folding of the precursor, (2) autoprocessing of the propeptide from the N terminus and (3) degradation of the cleaved propeptide. Using subt...

journal_title：Journal of molecular biology

authors： Shinde U,Inouye M

更新日期：1995-09-08 00:00:00

abstract：:The solution structure of r-elafin, a specific elastase inhibitor, has been determined using NMR spectroscopy. Characterized by a flat core and a flexible N-terminal extremity, the three-dimensional structure is formed by a central twisted beta-hairpin accompanied by two external segments linked by the proteinase bind...

journal_title：Journal of molecular biology

authors： Francart C,Dauchez M,Alix AJ,Lippens G

更新日期：1997-05-09 00:00:00

abstract：:Two crystal habits, one rod shaped and the other square prismatic, of the Fab fragment of a monoclonal anti-phenylalanine hydroxylase antibody have been grown using the method of vapour phase diffusion against polyethylene glycol 6000. The square prisms diffract to better than 2.8 A, belong to the space group P1 and h...

journal_title：Journal of molecular biology

authors： Guddat LW,Cowan SW,Machin KJ,Isaacs NW,Cotton RG

更新日期：1985-11-20 00:00:00

abstract：:We have previously shown that transcription of the Xenopus U6 snRNA gene by RNA polymerase III is stimulated in injected Xenopus oocytes by an activator element termed the DSE, which contains an octamer sequence. Data presented here reveal that the DSE contains, in addition, a GC-rich sequence capable of binding Sp1. ...

journal_title：Journal of molecular biology

authors： Lescure A,Tebb G,Mattaj IW,Krol A,Carbon P

更新日期：1992-11-20 00:00:00

abstract：:Transcription initiation by the enhancer-dependent sigma(54) RNA polymerase holoenzyme is positively regulated after promoter binding. The promoter DNA melting process is subject to activation by an enhancer-bound activator protein with nucleoside triphosphate hydrolysis activity. Tethered iron chelate probes attached...

journal_title：Journal of molecular biology

authors： Wigneshweraraj SR,Chaney MK,Ishihama A,Buck M

更新日期：2001-03-02 00:00:00

abstract：:We have measured the solubility, and the rates of homogeneous and heterogeneous nucleation on sickle hemoglobin (HbS beta 6 Glu-->Val) additionally modified by site-directed mutagenesis to possess Ala rather than Leu at beta 88, which forms part of the receptor site for beta 6 Val in the sickle polymer. The solubility...

journal_title：Journal of molecular biology

authors： Cao Z,Liao D,Mirchev R,Martin de Llano JJ,Himanen JP,Manning JM,Ferrone FA

更新日期：1997-02-07 00:00:00

abstract：:The substrate-binding H-site of human glutathione transferase (GST) M2-2 was subjected to iterative saturation mutagenesis in order to obtain an efficient enzyme with the novel epoxide substrate indene 1,2-oxide. Residues 10, 116, and 210 were targeted, and the activities with the alternative substrates, benzyl isothi...

journal_title：Journal of molecular biology

authors： Norrgård MA,Mannervik B

更新日期：2011-09-09 00:00:00

abstract：:Myosin VI is a molecular motor that is thought to function both as a transporter and as a cytoskeletal anchor in vivo. Here we use optical tweezers to examine force generation by single molecules of myosin VI under physiological nucleotide concentrations. We find that myosin VI is an efficient transporter at loads of ...

journal_title：Journal of molecular biology

authors： Chuan P,Spudich JA,Dunn AR

更新日期：2011-01-07 00:00:00

abstract：:Microglia, the resident macrophages of the central nervous system, critically influence neural function during development and in adulthood. Microglia are also profoundly sensitive to insults to the brain to which they respond with process of activation that includes spectrum of changes in morphology, function, and ge...

journal_title：Journal of molecular biology

authors： Ferro A,Sheeler C,Rosa JG,Cvetanovic M

更新日期：2019-04-19 00:00:00

abstract：:Three-dimensional domain swapping occurs when two or more identical proteins exchange identical parts of their structure to generate an oligomeric unit. It affects proteins with diverse sequences and structures, and is expected to play important roles in evolution, functional regulation and even conformational disease...

journal_title：Journal of molecular biology

authors： Dehouck Y,Biot C,Gilis D,Kwasigroch JM,Rooman M

更新日期：2003-07-25 00:00:00

abstract：:Elongation factor G (EF-G) is a large, five domain GTPase that catalyses the translocation of the tRNAs on the bacterial ribosome at the expense of GTP. In the crystal structure of GDP-bound EF-G, domain 1 (G domain) makes direct contacts with domains 2 and 5, whereas domain 4 protrudes from the body of the molecule. ...

journal_title：Journal of molecular biology

authors： Savelsbergh A,Matassova NB,Rodnina MV,Wintermeyer W

更新日期：2000-07-21 00:00:00

abstract：:The tyrosine residues adjacent to the C termini of the hemoglobin (Hb) subunits, alphaY140 and betaY145, are expected to play important structural roles, because the C termini are the loci of T-state quaternary salt-bridges, and because the tyrosine side-chains bridge the H and F helices via H bonds to the alphaV93 an...

journal_title：Journal of molecular biology

authors： Kneipp J,Balakrishnan G,Chen R,Shen TJ,Sahu SC,Ho NT,Giovannelli JL,Simplaceanu V,Ho C,Spiro TG

更新日期：2006-02-17 00:00:00

abstract：:S100P is a small calcium-binding protein of the S100 EF-hand-containing family of proteins. Elevated levels of its mRNA are reported to be associated with the progression to hormone independence and metastasis of prostate cancer and to be associated with loss of senescence in human breast epithelial cells in vitro. Th...

journal_title：Journal of molecular biology

authors： Zhang H,Wang G,Ding Y,Wang Z,Barraclough R,Rudland PS,Fernig DG,Rao Z

更新日期：2003-01-24 00:00:00

abstract：:The crystal structure of the allosteric tetrameric hemoglobin from Scapharca inaequivalvis (HbII) has been determined in the carbonmonoxy liganded state using a combination of anomalous scattering and molecular replacement. The molecular model has been refined at 2.0 A resolution to a conventional R-factor of 0.173 an...

journal_title：Journal of molecular biology

authors： Royer WE Jr,Heard KS,Harrington DJ,Chiancone E

更新日期：1995-10-13 00:00:00

abstract：:alpha-Lactalbumin (alpha-LA) is a two-domain, calcium-binding protein that forms one of the best studied molten globules. We present here amide hydrogen exchange studies of the molten globule formed by human alpha-LA at pH 2 and compare these results with a similar study of the native state at pH 6.3. The most persist...

journal_title：Journal of molecular biology

authors： Schulman BA,Redfield C,Peng ZY,Dobson CM,Kim PS

更新日期：1995-11-10 00:00:00

abstract：:The sequence-specific assignment of resonances is considered to be a requirement for the determination of the three-dimensional (3D) structure of a protein in solution by nuclear magnetic resonance methods. The main source of structural information is the nuclear Overhauser effect spectroscopy (NOESY) spectrum, which ...

journal_title：Journal of molecular biology

authors： Kraulis PJ

更新日期：1994-11-04 00:00:00