In vitro selection of RNAs aminoacylated by Escherichia coli leucyl-tRNA synthetase.

abstract：:We present evidence that the transcription termination factor Rho is autogenously regulated in Escherichia coli. The steady-state level of Rho is increased approximately tenfold in rho mutant cells. In the rho+ revertants, the content of Rho is similar to the wild-type level. A rho-/rho+ merodiploid produces equimolar...

journal_title：Journal of molecular biology

authors： Barik S,Bhattacharya P,Das A

更新日期：1985-04-20 00:00:00

abstract：:The enzyme penicillin acylase (penicillin amidohydrolase EC 3.5.1. 11) catalyses the cleavage of the amide bond in the benzylpenicillin (penicillin G) side-chain to produce phenylacetic acid and 6-aminopenicillanic acid (6-APA). The enzyme is of great pharmaceutical importance, as the product 6-APA is the starting poi...

journal_title：Journal of molecular biology

authors： Done SH,Brannigan JA,Moody PC,Hubbard RE

更新日期：1998-11-27 00:00:00

abstract：:Pathogenic strains of Helicobacter pylori use a type IV secretion system (T4SS) to deliver the toxin CagA into human host cells. The T4SS, along with the toxin itself, is coded into a genomic insert, which is termed the cag pathogenicity island. The cag pathogenicity island contains about 30 open-reading frames, for m...

journal_title：Journal of molecular biology

authors： Cendron L,Couturier M,Angelini A,Barison N,Stein M,Zanotti G

更新日期：2009-02-13 00:00:00

abstract：:Skeletal muscle development requires the coordinated expression of numerous transcription factors to control the specification of the muscle fate in mesodermal cells and the differentiation of the committed myoblasts into functional contractile fibers. The bHLH transcription factor MyoD plays a key role in these proce...

journal_title：Journal of molecular biology

authors： Bean C,Salamon M,Raffaello A,Campanaro S,Pallavicini A,Lanfranchi G

更新日期：2005-06-03 00:00:00

abstract：:A normal mode and energy minimization of ras p21 is used to determine the flexibility of the protein and the origin of the conformational differences between GTP and GDP-bound forms. To preserve the integrity of the structures, a hydration shell of water molecules was included as part of the system. Certain low-freque...

journal_title：Journal of molecular biology

authors： Ma J,Karplus M

更新日期：1997-11-21 00:00:00

abstract：:The genes coding for translation initiation factor IF3 (infC) and for the ribosomal proteins L35 (rpmI) and L20 (rplT) are transcribed in that order from a promoter in front of infC. The last two cistrons of the operon (rpmI and rplT) can be transcribed from a weak secondary promoter situated within the first cistron ...

journal_title：Journal of molecular biology

authors： Lesage P,Truong HN,Graffe M,Dondon J,Springer M

更新日期：1990-06-05 00:00:00

abstract：:A mutant version of the N-terminal domain of Escherichia coli DnaB helicase was used as a model system to assess the stabilization against unfolding gained by covalent cyclization. Cyclization was achieved in vivo by formation of an amide bond between the N and C termini with the help of a split mini-intein. Linear an...

journal_title：Journal of molecular biology

authors： Williams NK,Liepinsh E,Watt SJ,Prosselkov P,Matthews JM,Attard P,Beck JL,Dixon NE,Otting G

更新日期：2005-03-04 00:00:00

abstract：:Factor B is a key component of the alternative pathway of complement and is cleaved by factor D into the Ba and Bb fragments in the presence of activated C3 (C3b or C3(H(2)O)). The Ba fragment contains three short consensus/complement repeat domains, while the Bb fragment contains a von Willebrand factor type A (vWF-A...

journal_title：Journal of molecular biology

authors： Hinshelwood J,Perkins SJ

更新日期：2000-09-01 00:00:00

abstract：:Natural killer (NK) cells play a crucial role in the detection and destruction of virally infected and tumor cells during innate immune responses. The cytolytic activity of NK cells is regulated through a balance of inhibitory and stimulatory signals delivered by NK receptors that recognize classical major histocompat...

journal_title：Journal of molecular biology

authors： Dimasi N,Sawicki MW,Reineck LA,Li Y,Natarajan K,Margulies DH,Mariuzza RA

更新日期：2002-07-12 00:00:00

abstract：:The X-ray crystal structure of a catalytic-site mutant EQ208 [Glu208-->Gln] of alpha-amylase from Bacillus subtilis cocrystallized with maltopentaose (G5) and acarbose has been determined by multiple isomorphous replacement at 2.5 A resolution. Restrained crystallographic refinement has resulted in an R-factor of 19.8...

journal_title：Journal of molecular biology

authors： Fujimoto Z,Takase K,Doui N,Momma M,Matsumoto T,Mizuno H

更新日期：1998-03-27 00:00:00

abstract：:Microglia, the resident macrophages of the central nervous system, critically influence neural function during development and in adulthood. Microglia are also profoundly sensitive to insults to the brain to which they respond with process of activation that includes spectrum of changes in morphology, function, and ge...

journal_title：Journal of molecular biology

authors： Ferro A,Sheeler C,Rosa JG,Cvetanovic M

更新日期：2019-04-19 00:00:00

abstract：:The 14-3-3 family of proteins plays a role in a wide variety of cellular functions including regulation of protein kinase C and exocytosis. Using antisera specific for the N termini of 14-3-3 isoforms described previously and an additional antiserum specific for the C terminus of epsilon isoform, protease digestion of...

journal_title：Journal of molecular biology

authors： Jones DH,Martin H,Madrazo J,Robinson KA,Nielsen P,Roseboom PH,Patel Y,Howell SA,Aitken A

更新日期：1995-01-27 00:00:00

abstract：:Four new crystal forms of the anti-T lectin from jackfruit (Artocarpus integrifolia) have been prepared and characterized. Three of them, two monoclinic (P21, a = 59.4 A, b = 83.3 A, c = 63.5 A, beta = 107.7 degrees; C2, a = 106.1 A, b = 53.9 A, c = 128.0 A, beta = 95.0 A) and one orthorhombic (C222(1), a = 98.1 A, b ...

journal_title：Journal of molecular biology

authors： Banerjee R,Dhanaraj V,Mahanta SK,Surolia A,Vijayan M

更新日期：1991-10-05 00:00:00

abstract：:Many essential biological processes are mediated by complex molecular machines comprising multiple subunits. Knowledge on the architecture of individual subunits and their positions within the overall multimeric complex is key to understanding the molecular mechanisms of macromolecular assemblies. The anaphase-promoti...

journal_title：Journal of molecular biology

authors： Cronin NB,Yang J,Zhang Z,Kulkarni K,Chang L,Yamano H,Barford D

更新日期：2015-10-09 00:00:00

abstract：:Fibrillin-rich microfibrils are the major structural components of the extracellular matrix that provide elasticity in a majority of connective tissues. The basis of elastic properties lies in the organization of fibrillin molecules, which, unfortunately, is still poorly understood. An X-ray diffraction study of hydra...

journal_title：Journal of molecular biology

authors： Glab J,Wess T

更新日期：2008-11-28 00:00:00

abstract：:In order to predict the structure of alpha-helical coiled-coil proteins from their sequences, it is necessary to know how the side-chains pack in the interface between the alpha-helical strands. Since in alpha-fibrous proteins leucine is the most common residue at both the a and d positions of the heptad repeat, which...

journal_title：Journal of molecular biology

authors： Offer G,Sessions R

更新日期：1995-06-23 00:00:00

abstract：:Long terminal repeat elements and retroviruses require primers for initiation of minus and plus-strand DNA synthesis by reverse transcriptase. Here we demonstrate genetically that plus-strand DNA synthesis of the yeast Ty1 element is initiated at two sites located at the 5' boundary of the 3' long terminal repeat (PPT...

journal_title：Journal of molecular biology

authors： Heyman T,Agoutin B,Friant S,Wilhelm FX,Wilhelm ML

更新日期：1995-10-20 00:00:00

abstract：:The X-ray crystal structure of a complex of a modified recombinant kringle-2 domain of human plasminogen, K2Pg[C4G/E56D/L72Y] (mK2Pg), containing an upregulated lysine-binding site, bound to a functional 30 residue internal peptide (VEK-30) from an M-type protein of a group A Streptococcus surface protein, has been de...

journal_title：Journal of molecular biology

authors： Rios-Steiner JL,Schenone M,Mochalkin I,Tulinsky A,Castellino FJ

更新日期：2001-05-11 00:00:00

abstract：:Abasic lesions are common mutagenic or cytotoxic DNA damages. Ape1 is the major human apurinic/apyrimidinic (AP) endonuclease and initiates repair of abasic sites by catalyzing strand cleavage at the lesion. I show here that Ape1 single-stranded (ss) AP site incision activity prefers 0.5 mM or 2 mM MgCl(2) and low con...

journal_title：Journal of molecular biology

authors： Wilson DM 3rd

更新日期：2005-02-04 00:00:00

abstract：:The pyrogenic toxin toxic shock syndrome toxin-1 from Staphylococcus aureus is a causative agent of the toxic shock syndrome disease. It belongs to a family of proteins known as superantigens that cross-link major histocompatibility class II molecules and T-cell receptors leading to the activation of a substantial num...

journal_title：Journal of molecular biology

authors： Papageorgiou AC,Brehm RD,Leonidas DD,Tranter HS,Acharya KR

更新日期：1996-07-26 00:00:00

abstract：:The structure of basic Winged Bean Agglutinin (WBAI) with two dimeric molecules complexed with methyl-alpha-D-galactopyranoside in the asymmetric unit, has been determined by the molecular replacement method and refined with 2.5 A X-ray intensity data. The polypeptide chain of each monomer has the characteristic legum...

journal_title：Journal of molecular biology

authors： Prabu MM,Sankaranarayanan R,Puri KD,Sharma V,Surolia A,Vijayan M,Suguna K

更新日期：1998-03-06 00:00:00

abstract：:Type IV pili are multifunctional filaments displayed on many bacterial pathogens. Members of the Type IVa pilus subclass are found on a diverse group of human pathogens, whereas Type IVb pili are found almost exclusively on enteric bacteria. The Type IVa and IVb subclasses are distinguished by differences in the pilin...

journal_title：Journal of molecular biology

authors： Li J,Egelman EH,Craig L

更新日期：2012-04-20 00:00:00

abstract：:The insertion sequence IS903 encodes a 307 amino acid residue protein, transposase, that is essential for transposition. It is a multi-functional DNA-binding protein that specifically recognizes the 18 bp inverted repeats at the ends of the element and also recognizes DNa non-specifically when it captures a target sit...

journal_title：Journal of molecular biology

authors： Tavakoli NP,DeVost J,Derbyshire KM

更新日期：1997-12-12 00:00:00

abstract：:The enzyme mercuric ion reductase MerA is the central component of bacterial mercury resistance encoded by the mer operon. Many MerA proteins possess metallochaperone-like N-terminal domains (NmerA) that can transfer Hg(2+) to the catalytic core domain (Core) for reduction to Hg(0). These domains are tethered to the h...

journal_title：Journal of molecular biology

authors： Johs A,Harwood IM,Parks JM,Nauss RE,Smith JC,Liang L,Miller SM

更新日期：2011-10-28 00:00:00

abstract：:Retroviral proteases are translated as a part of Gag-related polyproteins, and are released and activated during particle release. Mason-Pfizer monkey virus (M-PMV) Gag polyproteins assemble into immature capsids within the cytoplasm of the host cells; however, their processing occurs only after transport to the plasm...

journal_title：Journal of molecular biology

authors： Zábranská H,Tůma R,Kluh I,Svatos A,Ruml T,Hrabal R,Pichová I

更新日期：2007-02-02 00:00:00

abstract：:The phycobilisome light-harvesting antenna in cyanobacteria and red algae is assembled from two substructures: a central core composed of allophycocyanin surrounded by rods that always contain phycocyanin (PC). Unpigmented proteins called linkers are also found within the rods and core. We present here two new structu...

journal_title：Journal of molecular biology

authors： David L,Marx A,Adir N

更新日期：2011-01-07 00:00:00

abstract：:As high-resolution cryogenic electron microscopy (cryo-EM) structures of ribosomes proliferate, at resolutions that allow atomic interactions to be visualized, this article attempts to give a perspective on the way research on ribosome structure and dynamics may be headed, and particularly the new opportunities we hav...

journal_title：Journal of molecular biology

authors： Frank J

更新日期：2016-09-11 00:00:00

abstract：:The crystal structure of cholesterol oxidase, a 56kDa flavoenzyme was anisotropically refined to 0.95A resolution. The final crystallographic R-factor and R(free) value is 11.0% and 13.2%, respectively. The quality of the electron density maps has enabled modeling of alternate conformations for 83 residues in the enzy...

journal_title：Journal of molecular biology

authors： Lario PI,Sampson N,Vrielink A

更新日期：2003-03-07 00:00:00

abstract：:Amyloid deposits in the pancreatic islets of Langerhans are thought to be a main factor responsible for death of the insulin-producing islet beta-cells in type 2 diabetes. It is hypothesized that beta-cell death is related to interaction of the 37 amino acid residue human islet amyloid polypeptide (hIAPP), the major c...

journal_title：Journal of molecular biology

authors： Engel MF,Yigittop H,Elgersma RC,Rijkers DT,Liskamp RM,de Kruijff B,Höppener JW,Antoinette Killian J

更新日期：2006-02-24 00:00:00

abstract：:The crystal structure of a class A beta-lactamase from Staphylococcus aureus PC1 has been refined at 2.0 A resolution. The resulting crystallographic R-factor (R = sigma h parallel Fo[-]Fc parallel/sigma h[Fo], where [Fo] and [Fc] are the observed and calculated structure factor amplitudes, respectively), is 0.163 for...

journal_title：Journal of molecular biology

authors： Herzberg O

更新日期：1991-02-20 00:00:00