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Preparation and X-ray characterization of four new crystal forms of jacalin, a lectin from Artocarpus integrifolia.

Abstract：

:Four new crystal forms of the anti-T lectin from jackfruit (Artocarpus integrifolia) have been prepared and characterized. Three of them, two monoclinic (P21, a = 59.4 A, b = 83.3 A, c = 63.5 A, beta = 107.7 degrees; C2, a = 106.1 A, b = 53.9 A, c = 128.0 A, beta = 95.0 A) and one orthorhombic (C222(1), a = 98.1 A, b = 67.3 A, c = 95.1 A) were grown with 2-methylpentan-2,4-diol (MPD) as the precipitant while the fourth, an hexagonal form (P6(1)22, a = b = 129.6 A, c = 157.9 A), was obtained in the presence of methyl-alpha-D-galactopyranoside with polyethylene glycol 4000 as the precipitant. The reported relative molecular mass (Mr) of the lectin was found to be inconsistent with the solvent content of the crystals estimated using measured densities. The Mr was redetermined using size-exclusion chromatography in the presence of methyl-alpha-D-galactopyranoside and Ferguson-plot analysis of mobilities in polyacrylamide gel electrophoresis. The redetermined Mr (66,000) is consistent with the measured crystal densities. The orthorhombic and the hexagonal forms, which have one half molecule and one molecule, respectively, in the asymmetric unit, are suitable for high-resolution X-ray analysis.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Banerjee R,Dhanaraj V,Mahanta SK,Surolia A,Vijayan M

doi

10.1016/0022-2836(91)80174-s

subject

Has Abstract

pub_date

1991-10-05 00:00:00

pages

773-6

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(91)80174-S

journal_volume

221

pub_type

杂志文章

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