Derivation of a structural model for the c-myc IRES.

abstract：:Single crystals of cytochrome cd1 nitrite reductase from Thiosphaera pantotropha have been grown in the presence of ammonium sulphate by vapour diffusion techniques. Crystals are suitable for X-ray analysis and diffract to dmin = 1.5 A. They belong to space group P2(1) with unit cell dimensions of a = 107.8 A, b = 61....

journal_title：Journal of molecular biology

authors： Fülöp V,Moir JW,Ferguson SJ,Hajdu J

更新日期：1993-08-20 00:00:00

abstract：:The pH and ionic strength dependence of the states of aggregation of brome mosaic virus protein has been investigated by small angle neutron scattering, quasielastic light-scattering, analytical centrifugation and electron microscopy. At pH above neutrality, protein oligomers are found in dynamical equilibrium, compri...

journal_title：Journal of molecular biology

authors： Cuillel M,Zulauf M,Jacrot B

更新日期：1983-03-15 00:00:00

abstract：:The HNH motif is a small nucleic acid binding and cleavage module, widespread in metal finger endonucleases in all life kingdoms. Here we studied a non-specific endonuclease, the nuclease domain of ColE7 (N-ColE7), to decipher the role of the conserved asparagine and histidine residues in the HNH motif. We found, usin...

journal_title：Journal of molecular biology

authors： Huang H,Yuan HS

更新日期：2007-05-04 00:00:00

abstract：:The nuclear localized, multi-subunit COP9 complex (or COP9 signalosome) is a key developmental modulator involved in repression of photomorphogenesis. In an effort to further define the molecular actions of the COP9 complex, a yeast two hybrid interactive screen was undertaken to identify proteins that could directly ...

journal_title：Journal of molecular biology

authors： Kwok SF,Staub JM,Deng XW

更新日期：1999-01-08 00:00:00

abstract：:FLP is a conservative site-specific recombinase that is encoded by the 2 microns plasmid of the yeast, Saccharomyces cerevisiae. FLP is member of the integrase family of recombinases that mediate the recombination reaction through a Holliday intermediate. The FLP recognition target (FRT) sites lie within two 599 bp in...

journal_title：Journal of molecular biology

authors： Dixon JE,Sadowski PD

更新日期：1994-10-21 00:00:00

abstract：:The multidomain Mac-2 binding protein (M2BP) is present in serum and in the extracellular matrix in the form of linear and ring-shaped oligomers, which interact with galectin-3, fibronectin, collagens, integrins and other large glycoproteins. Domain 1 of M2BP (M2BP-1) shows homology with the cysteine-rich SRCR domain ...

journal_title：Journal of molecular biology

authors： Müller SA,Sasaki T,Bork P,Wolpensinger B,Schulthess T,Timpl R,Engel A,Engel J

更新日期：1999-08-27 00:00:00

abstract：:Small multidrug resistance (SMR) transporters contribute to bacterial resistance by coupling the efflux of a wide range of toxic aromatic cations, some of which are commonly used as antibiotics and antiseptics, to proton influx. EmrE is a prototypical small multidrug resistance transporter comprising four transmembran...

journal_title：Journal of molecular biology

authors： Fleishman SJ,Harrington SE,Enosh A,Halperin D,Tate CG,Ben-Tal N

更新日期：2006-11-17 00:00:00

abstract：:The x-ray structure of LeuT, a bacterial homologue of Na(+)/Cl(-)-dependent neurotransmitter transporters, provides a great opportunity to better understand the molecular basis of monovalent cation selectivity in ion-coupled transporters. LeuT possesses two ion binding sites, NA1 and NA2, which are highly selective fo...

journal_title：Journal of molecular biology

authors： Noskov SY,Roux B

更新日期：2008-03-28 00:00:00

abstract：:Ribosomes are dynamic complexes responsible for translating the genetic information encoded in mRNAs to proteins. The accuracy of this process is vital to the survival of an organism, and is often compromised by translational miscoding agents. Aminoglycosides are a group of miscoding agents that bind to the ribosome a...

journal_title：Journal of molecular biology

authors： Jerinic O,Joseph S

更新日期：2000-12-15 00:00:00

abstract：:Tannins are water-soluble polyphenolic compounds in plants. Hydrolyzable tannins are derivatives of gallic acid (3,4,5-trihydroxybenzoic acid) or its meta-depsidic forms that are esterified to polyol, catechin, or triterpenoid units. Tannases are a family of esterases that catalyze the hydrolysis of the galloyl ester ...

journal_title：Journal of molecular biology

authors： Ren B,Wu M,Wang Q,Peng X,Wen H,McKinstry WJ,Chen Q

更新日期：2013-08-09 00:00:00

abstract：:The conversion of specific proteins or protein fragments into insoluble, ordered fibrillar aggregates is a fundamental process in protein chemistry, biology, medicine and biotechnology. As this structural conversion seems to be a property shared by many proteins, understanding the mechanism of this process will be of ...

journal_title：Journal of molecular biology

authors： Campioni S,Mossuto MF,Torrassa S,Calloni G,de Laureto PP,Relini A,Fontana A,Chiti F

更新日期：2008-06-06 00:00:00

abstract：:Solvent binding in the nucleosome core particle containing a 147 base pair, defined-sequence DNA is characterized from the X-ray crystal structure at 1.9 A resolution. A single-base-pair increase in DNA length over that used previously results in substantially improved clarity of the electron density and accuracy for ...

journal_title：Journal of molecular biology

authors： Davey CA,Sargent DF,Luger K,Maeder AW,Richmond TJ

更新日期：2002-06-21 00:00:00

abstract：:The pre-steady-state kinetics of GTP hydrolysis catalysed by elongation factor G and ribosomes from Escherichia coli has been investigated by the method of quenched-flow. The GTPase activities either uncoupled from or coupled to the ribosomal translocation process were characterized under various experimental conditio...

journal_title：Journal of molecular biology

authors： Robertson JM,Urbanke C,Chinali G,Wintermeyer W,Parmeggiani A

更新日期：1986-06-20 00:00:00

abstract：:Cyclin K and the closely related cyclins T1, T2a, and T2b interact with cyclin-dependent kinase 9 (CDK9) forming multiple nuclear complexes, referred to collectively as positive transcription elongation factor b (P-TEFb). Through phosphorylation of the C-terminal domain of the RNA polymerase II largest subunit, distin...

journal_title：Journal of molecular biology

authors： Baek K,Brown RS,Birrane G,Ladias JA

更新日期：2007-02-16 00:00:00

abstract：:Polyglutamine (polyQ) amyloid fibrils are observed in disease tissue and have been implicated as toxic agents responsible for neurodegeneration in expanded CAG repeat diseases such as Huntington's disease. Despite intensive efforts, the mechanism of amyloid toxicity remains unknown. As a novel approach to probing poly...

journal_title：Journal of molecular biology

authors： Kar K,Arduini I,Drombosky KW,van der Wel PC,Wetzel R

更新日期：2014-02-20 00:00:00

abstract：:The pH dependence of the amide/solvent hydrogen exchange of individual amide groups in hen egg-white lysozyme has been studied by nuclear magnetic resonance spectroscopy. Lysozyme has been used here as a model for a globular protein to re-examine the hypothesis for the amide/solvent hydrogen exchange reaction proposed...

journal_title：Journal of molecular biology

authors： Pedersen TG,Thomsen NK,Andersen KV,Madsen JC,Poulsen FM

更新日期：1993-03-20 00:00:00

abstract：:Mitochondrial DNA (mtDNA) sequence variation can influence the penetrance of complex diseases and climatic adaptation. While studies in geographically defined human populations suggest that mtDNA mutations become fixed when they have conferred metabolic capabilities optimally suited for a specific environment, it has ...

journal_title：Journal of molecular biology

authors： Dingley SD,Polyak E,Ostrovsky J,Srinivasan S,Lee I,Rosenfeld AB,Tsukikawa M,Xiao R,Selak MA,Coon JJ,Hebert AS,Grimsrud PA,Kwon YJ,Pagliarini DJ,Gai X,Schurr TG,Hüttemann M,Nakamaru-Ogiso E,Falk MJ

更新日期：2014-05-29 00:00:00

abstract：:A shortened derivative of the group IIA intron from the mitochondrial cytochrome-c-oxidase subunit I gene (COI I1) of the ascomycete Podospora anserina can undergo self-splicing in vitro. When compared to self-splicing group IIB introns from yeast mitochondria (aI5c, bI1) the autocatalytic reaction shows a lower effic...

journal_title：Journal of molecular biology

authors： Schmidt U,Sägebarth R,Schmelzer C,Stahl U

更新日期：1993-06-05 00:00:00

abstract：:Intercellular communication via gap junction membrane channels cannot occur until two apposing hemichannels (connexons) meet and dock to form a sealed cell-cell conduit. In particular, an important question is how does the structure at the extracellular surface influence the molecular recognition of the two connexons....

journal_title：Journal of molecular biology

authors： Perkins GA,Goodenough DA,Sosinsky GE

更新日期：1998-03-27 00:00:00

abstract：:The roles of the residues in the catalytic trio Glu212-Asp214-Glu217 in cellobiohydrolase I (CBHI) from Trichoderma reesei have been investigated by changing these residues to their isosteric amide counterparts. Three mutants, E212Q, D214N and E217Q, were constructed and expressed in T. reesei. All three point mutatio...

journal_title：Journal of molecular biology

authors： Ståhlberg J,Divne C,Koivula A,Piens K,Claeyssens M,Teeri TT,Jones TA

更新日期：1996-11-29 00:00:00

abstract：:Here, we report the molecular characterization of the human cytomegalovirus uracil DNA glycosylase (UNG) UL114. Purified UL114 was shown to be a DNA glycosylase, which removes uracil from double-stranded and single-stranded DNA. However, kinetic analysis has shown that viral UNG removed uracil more slowly compared wit...

journal_title：Journal of molecular biology

authors： Ranneberg-Nilsen T,Dale HA,Luna L,Slettebakk R,Sundheim O,Rollag H,Bjørås M

更新日期：2008-08-29 00:00:00

abstract：:The X-ray crystal structure of a complex of a modified recombinant kringle-2 domain of human plasminogen, K2Pg[C4G/E56D/L72Y] (mK2Pg), containing an upregulated lysine-binding site, bound to a functional 30 residue internal peptide (VEK-30) from an M-type protein of a group A Streptococcus surface protein, has been de...

journal_title：Journal of molecular biology

authors： Rios-Steiner JL,Schenone M,Mochalkin I,Tulinsky A,Castellino FJ

更新日期：2001-05-11 00:00:00

abstract：:The origin of metal ion selectivity by members of the SmtB/ArsR family of bacterial metal-sensing transcriptional repressors and the mechanism of negative allosteric regulation of DNA binding is poorly understood. Here, we report that two homologous zinc sensors, Staphylococcus aureus CzrA and cyanobacterial SmtB, are...

journal_title：Journal of molecular biology

authors： Eicken C,Pennella MA,Chen X,Koshlap KM,VanZile ML,Sacchettini JC,Giedroc DP

更新日期：2003-10-31 00:00:00

abstract：:The structure of the yeast DNA-dependent RNA polymerase I (RNA Pol I), prepared by cryo-negative staining, was studied by electron microscopy. A structural model of the enzyme at a resolution of 1.8 nm was determined from the analysis of isolated molecules and showed an excellent fit with the atomic structure of the R...

journal_title：Journal of molecular biology

authors： De Carlo S,Carles C,Riva M,Schultz P

更新日期：2003-06-20 00:00:00

abstract：:The crystal structure of cholesterol oxidase, a 56kDa flavoenzyme was anisotropically refined to 0.95A resolution. The final crystallographic R-factor and R(free) value is 11.0% and 13.2%, respectively. The quality of the electron density maps has enabled modeling of alternate conformations for 83 residues in the enzy...

journal_title：Journal of molecular biology

authors： Lario PI,Sampson N,Vrielink A

更新日期：2003-03-07 00:00:00

abstract：:Molten globules are partially folded states of proteins which are generally believed to mimic structures formed during the folding process. In order to determine the minimal requirements for the formation of a molten globule state, we have prepared a set of peptide models of the molten globule state of human alpha-lac...

journal_title：Journal of molecular biology

authors： Demarest SJ,Boice JA,Fairman R,Raleigh DP

更新日期：1999-11-19 00:00:00

abstract：:Historically referred to as "the GTPase center", the L11 binding region (L11BR) of Escherichia coli 23 S rRNA is a highly conserved structure that has been implicated in several essential functions during protein synthesis. Here, in vivo expression of an RNA fragment containing that structure was found to affect trans...

journal_title：Journal of molecular biology

authors： Van Dyke N,Xu W,Murgola EJ

更新日期：2002-05-31 00:00:00

abstract：:Mannan-binding lectin (MBL) is the archetypical pathogen recognition molecule of the innate immune defense. Upon binding to microorganisms, reactions leading to the destruction of the offender ensue. MBL is an oligomer of structural subunits each composed of three identical polypeptides. We used atomic force microscop...

journal_title：Journal of molecular biology

authors： Jensenius H,Klein DC,van Hecke M,Oosterkamp TH,Schmidt T,Jensenius JC

更新日期：2009-08-07 00:00:00

abstract：:An essential feature of viral quasispecies, predicted from quasispecies theory, is that the target of selection is the mutant distribution as a whole. To test molecularly the mutant composition selected from a viral quasispecies we reconstructed a mutant distribution using 19 antigenic variants of foot-and-mouth disea...

journal_title：Journal of molecular biology

authors： Perales C,Martín V,Ruiz-Jarabo CM,Domingo E

更新日期：2005-01-21 00:00:00

abstract：:Saccharomyces cerevisiae is an occasional host to an array of prions, most based on self-propagating, self-templating amyloid filaments of a normally soluble protein. [URE3] is a prion of Ure2p, a regulator of nitrogen catabolism, while [PSI+] is a prion of Sup35p, a subunit of the translation termination factor Sup35...

journal_title：Journal of molecular biology

authors： Wickner RB,Edskes HK,Son M,Bezsonov EE,DeWilde M,Ducatez M

更新日期：2018-10-12 00:00:00