WebPropagate: A Web Server for Network Propagation.

abstract：:Two crystal habits, one rod shaped and the other square prismatic, of the Fab fragment of a monoclonal anti-phenylalanine hydroxylase antibody have been grown using the method of vapour phase diffusion against polyethylene glycol 6000. The square prisms diffract to better than 2.8 A, belong to the space group P1 and h...

journal_title：Journal of molecular biology

authors： Guddat LW,Cowan SW,Machin KJ,Isaacs NW,Cotton RG

更新日期：1985-11-20 00:00:00

abstract：:The N-terminal SH3 domain of the Drosophila drk protein (drkN SH3) exists in equilibrium between folded and unfolded states under non-denaturing buffer conditions. In order to examine the origins of this instability, we have made mutations in the domain and characterized the thermodynamics and kinetics of folding. Res...

journal_title：Journal of molecular biology

authors： Mok YK,Elisseeva EL,Davidson AR,Forman-Kay JD

更新日期：2001-03-30 00:00:00

abstract：:The rotavirus double-layered particle (DLP) is a molecular machine that transcribes 11 genomic segments of double-stranded RNA into full-length mRNA segments during viral replication. DLPs from the human Wa strain of virus, belonging to subgroup II (SG II), possess a significantly reduced level of transcriptase activi...

journal_title：Journal of molecular biology

authors： Greig SL,Berriman JA,O'Brien JA,Taylor JA,Bellamy AR,Yeager MJ,Mitra AK

更新日期：2006-02-10 00:00:00

abstract：:Nucleocapsid assembly is an essential process in the replication of the non-segmented, negative-sense RNA viruses (NNVs). Unassembled nucleoprotein (N(0)) is maintained in an RNA-free and monomeric form by its viral chaperone, the phosphoprotein (P), forming the N(0)-P complex. Our earlier work solved the structure of...

journal_title：Journal of molecular biology

authors： Yabukarski F,Leyrat C,Martinez N,Communie G,Ivanov I,Ribeiro EA Jr,Buisson M,Gerard FC,Bourhis JM,Jensen MR,Bernadó P,Blackledge M,Jamin M

更新日期：2016-07-03 00:00:00

abstract：:Azaleucine is a naturally occurring amino acid antibiotic that can be incorporated into proteins by mimicking leucine. In spite of its highly toxic character, the contrast between the hydrophobic side-chain of leucine and the basic side-chain of azaleucine suggested a mechanism for rescuing certain mutants. We constru...

journal_title：Journal of molecular biology

authors： Lemeignan B,Sonigo P,Marlière P

更新日期：1993-05-20 00:00:00

abstract：:Though up to 20% of the total RNA in bacterial cells is tRNA, the regulation of tRNA distribution on the genomic level remains unclear. tRNA distribution is governed by four processes: transcription, processing of precursor tRNA, degradation of precursor tRNA and degradation of mature tRNA. To elucidate the relationsh...

journal_title：Journal of molecular biology

authors： Dittmar KA,Mobley EM,Radek AJ,Pan T

更新日期：2004-03-12 00:00:00

abstract：:In human metallothionein-2, the exchange rate constants of ten amide protons were found to range from 1.7 x 10(-4) to 1 x 10(-1) min-1 at pH 6.3 and 8 degrees C. Most of these slowly exchanging protons could be associated with hydrogen bonds in secondary structure elements of the alpha-domain. Amide proton exchange ra...

journal_title：Journal of molecular biology

authors： Messerle BA,Bos M,Schäffer A,Vasák M,Kägi JH,Wüthrich K

更新日期：1990-08-05 00:00:00

abstract：:The clarification of the physico-chemical determinants underlying amyloid deposition is critical for our understanding of misfolding diseases. With this purpose we have performed a systematic all-atom molecular dynamics (MD) study of a series of single point mutants of the de novo designed amyloidogenic peptide STVIIE...

journal_title：Journal of molecular biology

authors： López de la Paz M,de Mori GM,Serrano L,Colombo G

更新日期：2005-06-10 00:00:00

abstract：:The selective packaging of the primer tRNA(Lys3) into HIV-1 particles is dependent upon the viral incorporation of the Pr160gag-pol precursor protein. In order to map a tRNA(Lys3) binding site within this precursor, we have studied the effects of mutations in Pr160gag-pol upon the selective incorporation of tRNA(Lys3)...

journal_title：Journal of molecular biology

authors： Khorchid A,Javanbakht H,Wise S,Halwani R,Parniak MA,Wainberg MA,Kleiman L

更新日期：2000-05-26 00:00:00

abstract：:Historically referred to as "the GTPase center", the L11 binding region (L11BR) of Escherichia coli 23 S rRNA is a highly conserved structure that has been implicated in several essential functions during protein synthesis. Here, in vivo expression of an RNA fragment containing that structure was found to affect trans...

journal_title：Journal of molecular biology

authors： Van Dyke N,Xu W,Murgola EJ

更新日期：2002-05-31 00:00:00

abstract：:The enzyme encoded by the bgIA gene of Bacillus polymyxa, a type I beta-glucosidase belonging to family I of glycosyl hydrolases, has been purified to homogeneity from an Escherichia coli culture which overexpressed the gene, and crystallized. The crystals, which diffract to 3.0 A resolution, belong to the orthorhombi...

journal_title：Journal of molecular biology

authors： Sanz-Aparicio J,Romero A,Martinez-Ripoll M,Madarro A,Flors A,Polaina J

更新日期：1994-07-15 00:00:00

abstract：:Rabbit liver aldolase was purified by affinity elution on a CM52 ion exchanger. Crystals of rabbit liver aldolase suitable for X-ray diffraction experiments were grown from 45% saturated ammonium sulfate solution at 4 degrees C. The enzyme crystallizes in space group C222(1) having cell dimensions a = 377.02 A, b = 13...

journal_title：Journal of molecular biology

authors： Sygusch J,Beaudry D

更新日期：1985-11-05 00:00:00

abstract：:The FNR (fumarate nitrate reduction) protein plays a central role in the global oxygen response of a variety of bacteria. In Escherichia coli, FNR is the master transcriptional regulator of the transition between aerobic and anaerobic growth. Regulation of FNR is achieved by cycling the molecule between three states i...

journal_title：Journal of molecular biology

authors： Tolla DA,Savageau MA

更新日期：2010-04-09 00:00:00

abstract：:The beta-lactamases are involved in bacterial resistance to penicillin and related compounds. Members of the metallo-enzyme class are now found in many pathogenic bacteria and are thus becoming of major clinical importance. The structures of the Zn-beta-lactamase from Fluoribacter gormanii (FEZ-1) in the native and in...

journal_title：Journal of molecular biology

authors： García-Sáez I,Mercuri PS,Papamicael C,Kahn R,Frère JM,Galleni M,Rossolini GM,Dideberg O

更新日期：2003-01-24 00:00:00

abstract：:Protein allostery plays a pivotal role in many regulatory processes. Prominent examples are cell-surface receptors, which allosterically transmit ligand-generated signals to their cytoplasmic domains, or bacterial transcription factors, which alternate between a free conformation and a DNA-bound conformation in respon...

journal_title：Journal of molecular biology

authors： Goeke D,Kaspar D,Stoeckle C,Grubmüller S,Berens C,Klotzsche M,Hillen W

更新日期：2012-02-10 00:00:00

abstract：:tRNA isodecoders share the same anticodon but have differences in their body sequence. An unexpected result from genome sequencing projects is the identification of a large number of tRNA isodecoder genes in mammalian genomes. In the reference human genome, more than 270 isodecoder genes are present among the approxim...

journal_title：Journal of molecular biology

authors： Geslain R,Pan T

更新日期：2010-02-26 00:00:00

abstract：:We designed a shuttle vector system that allowed a comparison of the mutation spectrum on the supF target gene after transfection of single-stranded or double-stranded DNA into monkey cells. Single-strand-derived plasmids exhibited a spontaneous mutation frequency tenfold higher than double-strand-derived ones. These ...

journal_title：Journal of molecular biology

authors： Madzak C,Sarasin A

更新日期：1991-04-20 00:00:00

abstract：:The Flp recombinase of yeast and the Cre recombinase of bacteriophage P1 both belong to the lambda-integrase (Int) family of site-specific recombinases. These recombination systems recognize recombination-target sequences that consist of two 13bp inverted repeats flanking a 6 or 8bp spacer sequence. Recombination reac...

journal_title：Journal of molecular biology

authors： Vetcher AA,Lushnikov AY,Navarra-Madsen J,Scharein RG,Lyubchenko YL,Darcy IK,Levene SD

更新日期：2006-04-07 00:00:00

abstract：:Ribgrass mosaic virus (RMV) is a member of the tobamovirus group of plant viruses. The structure has been determined at 2.9 A resolution by fiber diffraction methods, and refined by molecular dynamics methods to an R-factor of 0.095. The carboxyl-carboxylate interactions that drive disassembly in tobamoviruses are pre...

journal_title：Journal of molecular biology

authors： Wang H,Culver JN,Stubbs G

更新日期：1997-06-27 00:00:00

abstract：:The Integrases of bacteriophages lambda and HK022 promote recombination between DNA molecules that carry attachment sites. The two integrases are about 70% identical in sequence and catalyze nearly identical reactions, but recognize different sets of sites. To identify the amino acids that determine this difference in...

journal_title：Journal of molecular biology

authors： Dorgai L,Yagil E,Weisberg RA

更新日期：1995-09-15 00:00:00

abstract：:A parallel stacking arrangement of the guanidinium groups of arginines directly over the center of the rings of aromatic side-chains is observed much more frequently in proteins than would be expected by chance. This type of interaction, which is often found in locations critical to the function, apparently serves to ...

journal_title：Journal of molecular biology

authors： Flocco MM,Mowbray SL

更新日期：1994-01-14 00:00:00

abstract：:The binding sites for histones and high mobility group proteins (HMG) 14 and 17 have been located on DNA in the nucleosomal cores and H1/H5-containing nucleosomes. The nucleosomes were specifically associated with two molecules of the non-histone proteins HMG 14 and/or HMG 17 when followed by DNA-protein crosslinking ...

journal_title：Journal of molecular biology

authors： Shick VV,Belyavsky AV,Mirzabekov AD

更新日期：1985-09-20 00:00:00

abstract：:It is becoming increasingly clear that the intrinsic and protein-induced topological properties of the DNA helix influence transcriptional efficiency. In this report we describe the properties of two upstream activating regions that influence transcription from the non-overlapping tandem promoters of the ilvGMEDA oper...

journal_title：Journal of molecular biology

authors： Pagel JM,Winkelman JW,Adams CW,Hatfield GW

更新日期：1992-04-20 00:00:00

abstract：:Filamentous bacteriophage assemble at the host membrane in a non-lytic process; the gene-3 minor coat protein (P3) is required for release from the membrane and subsequently, for recognition and infection of a new host. P3 contains at least three distinct domains: two N-terminal domains that mediate host recognition a...

journal_title：Journal of molecular biology

authors： Weiss GA,Roth TA,Baldi PF,Sidhu SS

更新日期：2003-09-26 00:00:00

abstract：:Cholera toxin (CT) holotoxin must be activated to intoxicate host cells. This process requires the intracellular dissociation of the enzymatic CTA1 domain from the holotoxin components CTA2 and B5, followed by subsequent interaction with the host factor ADP ribosylation factor 6 (ARF6)-GTP. We report the first NMR-bas...

journal_title：Journal of molecular biology

authors： Ampapathi RS,Creath AL,Lou DI,Craft JW Jr,Blanke SR,Legge GB

更新日期：2008-03-28 00:00:00

abstract：:The "International Symposium on Nucleotide Second Messenger Signaling in Bacteria" (September 30-October 3, 2018, Berlin), which was organized within the framework of DFG Priority Programme 1879 (www.spp1879.de), brought together 125 participants from 20 countries to discuss recent progress and future trends in this f...

journal_title：Journal of molecular biology

authors： Hengge R,Häussler S,Pruteanu M,Stülke J,Tschowri N,Turgay K

更新日期：2019-03-01 00:00:00

abstract：:We have studied the effect of organic co-solvents (monohydric alcohols and formamide) on the dimer-tetramer equilibrium of human haemoglobin by measuring the dependence of oxygen affinity upon haemoglobin concentration over a 100-fold concentration range and analysing the data with a modified Monod-Wyman-Changeux mode...

journal_title：Journal of molecular biology

authors： Cupane A,Vitrano E,Cordone L

更新日期：1986-05-20 00:00:00

abstract：:Bacterial microcompartments (BMCs) sequester enzymes from the cytoplasmic environment by encapsulation inside a selectively permeable protein shell. Bioinformatic analyses indicate that many bacteria encode BMC clusters of unknown function and with diverse combinations of shell proteins. The genome of the halophilic m...

journal_title：Journal of molecular biology

authors： Lassila JK,Bernstein SL,Kinney JN,Axen SD,Kerfeld CA

更新日期：2014-05-29 00:00:00

abstract：:Incubation of intact reticulocytes with chloramphenicol at concentrations over 10(-3) M caused inhibition of protein synthesis and conversion of polyribosomes to single ribosomes. At 3 x 10(-3) M-chloramphenicol these effects were completely reversible, but reversibility decreased as the chloramphenicol concentration ...

journal_title：Journal of molecular biology

authors： Godchaux W 3rd,Herbert E

更新日期：1966-11-28 00:00:00

abstract：:The Escherichia coli rap mutant inhibits vegetative growth of bacteriophage lambda. Phage mutations termed bar, which overcome the rap defect, have been mapped to three genetic loci in the pL operon. Plasmids with a lambda wild-type bar DNA segment cloned downstream from an active promoter cannot be maintained in rap ...

journal_title：Journal of molecular biology

authors： Pérez-Morga D,Guarneros G

更新日期：1990-11-20 00:00:00