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Amide proton exchange in human metallothionein-2 measured by nuclear magnetic resonance spectroscopy.

Abstract：

:In human metallothionein-2, the exchange rate constants of ten amide protons were found to range from 1.7 x 10(-4) to 1 x 10(-1) min-1 at pH 6.3 and 8 degrees C. Most of these slowly exchanging protons could be associated with hydrogen bonds in secondary structure elements of the alpha-domain. Amide proton exchange rates thus present an additional criterion for the structural characterization of different metallothioneins, which could be particularly valuable for comparisons of different homologous protein preparations containing nuclear magnetic resonance-inactive metal ions, where the metal-polypeptide co-ordinative bonds cannot be identified directly.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Messerle BA,Bos M,Schäffer A,Vasák M,Kägi JH,Wüthrich K

doi

10.1016/0022-2836(90)90292-T

subject

Has Abstract

pub_date

1990-08-05 00:00:00

pages

781-6

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(90)90292-T

journal_volume

214

pub_type

杂志文章

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