Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of Bacillus polymyxa.

abstract：:The observed sequence dependence of the mean twist angles in 38 B-DNA crystal structures can be understood in terms of simple geometrical features of the constituent base-pairs. Structures with low twist appear to unwind in response to severe steric clashes of large exocyclic groups (such as NH2-NH2) in the major and ...

journal_title：Journal of molecular biology

authors： Gorin AA,Zhurkin VB,Olson WK

更新日期：1995-03-17 00:00:00

abstract：:Transcription by RNA polymerase II (Pol II) is required to produce mRNAs and some noncoding RNAs (ncRNAs) within mammalian cells. This coordinated process is precisely regulated by multiple factors, including many recently discovered ncRNAs. In this perspective, we will discuss newly identified ncRNAs that facilitate ...

journal_title：Journal of molecular biology

authors： Eidem TM,Kugel JF,Goodrich JA

更新日期：2016-06-19 00:00:00

abstract：:Long terminal repeat elements and retroviruses require primers for initiation of minus and plus-strand DNA synthesis by reverse transcriptase. Here we demonstrate genetically that plus-strand DNA synthesis of the yeast Ty1 element is initiated at two sites located at the 5' boundary of the 3' long terminal repeat (PPT...

journal_title：Journal of molecular biology

authors： Heyman T,Agoutin B,Friant S,Wilhelm FX,Wilhelm ML

更新日期：1995-10-20 00:00:00

abstract：:Expression of the plant protein centroradialis (CEN) leads to a morphological switch between shoot growth and the development of flower structures (inflorescence). We have determined the crystal structure of Antirrhinum CEN to 1.9 A resolution. This structure confirms the CEN proteins as a subset of the family of phos...

journal_title：Journal of molecular biology

authors： Banfield MJ,Brady RL

更新日期：2000-04-14 00:00:00

abstract：:Crystals of an almost self-complementary DNA 15-mer d(CGCGAAATTTACGCG) have been grown by the vapor diffusion technique at 4 degrees C. The space group is I222 with a = 37.3 A, b = 54.6 A and c = 104.8 A. Solution studies showed that the 15-mer forms a duplex with the extra adenine residue unpaired: (sequence; see tex...

journal_title：Journal of molecular biology

authors： Miller M,Wlodawer A,Appella E,Sussman JL

更新日期：1987-06-20 00:00:00

abstract：:Activation of family A G-protein-coupled receptors involves a rearrangement of a conserved interhelical cytoplasmic hydrogen bond network between the E(D)RY motif on transmembrane helix 3 (H3) and residues on H6, which is commonly termed the cytoplasmic "ionic lock." Glu134(3.49) of the E(D)RY motif also forms an intr...

journal_title：Journal of molecular biology

authors： Vogel R,Mahalingam M,Lüdeke S,Huber T,Siebert F,Sakmar TP

更新日期：2008-07-18 00:00:00

abstract：:The Gasdermin (GSDM) family consists of Gasdermin A (GSDMA), Gasdermin B (GSDMB), Gasdermin C (GSDMC), Gasdermin D (GSDMD), Gasdermin E (GSDME) and Pejvakin (PJVK). GSDMD is activated by inflammasome-associated inflammatory caspases. Cleavage of GSDMD by human or mouse caspase-1, human caspase-4, human caspase-5, and ...

journal_title：Journal of molecular biology

authors： Feng S,Fox D,Man SM

更新日期：2018-09-14 00:00:00

abstract：:Solvent binding in the nucleosome core particle containing a 147 base pair, defined-sequence DNA is characterized from the X-ray crystal structure at 1.9 A resolution. A single-base-pair increase in DNA length over that used previously results in substantially improved clarity of the electron density and accuracy for ...

journal_title：Journal of molecular biology

authors： Davey CA,Sargent DF,Luger K,Maeder AW,Richmond TJ

更新日期：2002-06-21 00:00:00

abstract：:A restriction fragment carrying the major coat protein gene (gene VIII) was excised from the DNA of the class I filamentous bacteriophage fd, which infects Escherichia coli. This fragment was cloned into the expression plasmid pKK223-3, where it came under the control of the tac promoter, generating plasmid pKf8P. Bac...

journal_title：Journal of molecular biology

authors： Rowitch DH,Hunter GJ,Perham RN

更新日期：1988-12-05 00:00:00

abstract：:Prions are the agents of a series of lethal neurodegenerative diseases. They are composed largely, if not entirely, of the host-encoded prion protein (PrP), which can exist in the cellular isoform PrP(C) and the pathological isoform PrP(Sc). The conformational change of the alpha-helical PrP(C) into beta-sheet-rich Pr...

journal_title：Journal of molecular biology

authors： Kaimann T,Metzger S,Kuhlmann K,Brandt B,Birkmann E,Höltje HD,Riesner D

更新日期：2008-02-15 00:00:00

abstract：:The metazoan cell membrane is highly organized. Maintaining such organization and preserving membrane integrity under different conditions are accomplished through intracellular tethering to an extensive, flexible protein network. Spectrin, the principal component of this network, is attached to the membrane through t...

journal_title：Journal of molecular biology

authors： Yasunaga M,Ipsaro JJ,Mondragón A

更新日期：2012-04-06 00:00:00

abstract：:We have determined the X-ray crystallographic structure of trout Hb I in both the deoxy and carbonmonoxy forms to resolution limits of 2.3 angstroms and 2.5 angstroms, respectively. The overall fold of the molecule is highly similar to that of human HbA despite the low level of sequence identity between these proteins...

journal_title：Journal of molecular biology

authors： Tame JR,Wilson JC,Weber RE

更新日期：1996-06-21 00:00:00

abstract：:Muscle-specific kinase (MuSK) is a receptor tyrosine kinase expressed exclusively in skeletal muscle, where it is required for formation of the neuromuscular junction. MuSK is activated by agrin, a neuron-derived heparan sulfate proteoglycan. Here, we report the crystal structure of the agrin-responsive first and seco...

journal_title：Journal of molecular biology

authors： Stiegler AL,Burden SJ,Hubbard SR

更新日期：2006-12-01 00:00:00

abstract：:The Listeria monocytogenes surface protein InlB mediates bacterial invasion into host cells by activating the human receptor tyrosine kinase Met. So far, it is unknown how InlB or the physiological Met ligand hepatocyte growth factor/scatter factor causes Met dimerization, which is considered a prerequisite for recept...

journal_title：Journal of molecular biology

authors： Ferraris DM,Gherardi E,Di Y,Heinz DW,Niemann HH

更新日期：2010-01-22 00:00:00

abstract：:The majority of archaeal viral genes are of unknown function hindering our understanding of the virus life cycle and viral interactions with their host. Here, we first describe functional characterization of ORF131b (gp17) and ORF436 (gp18) of Sulfolobus islandicus rod-shaped virus 2 (SIRV2), both encoding proteins of...

journal_title：Journal of molecular biology

authors： Guo Y,Kragelund BB,White MF,Peng X

更新日期：2015-06-19 00:00:00

abstract：:The family of fibroblast growth factor receptors (FGFRs) plays an important and well-characterized role in a variety of pathological disorders. FGFR4 is involved in myogenesis and muscle regeneration. Mutations affecting the kinase domain of FGFR4 may cause cancer, for example, breast cancer or rhabdomyosarcoma. Where...

journal_title：Journal of molecular biology

authors： Lesca E,Lammens A,Huber R,Augustin M

更新日期：2014-11-11 00:00:00

abstract：:Prions are composed largely, if not entirely, of the scrapie isoform of the prion protein (PrPSc). Conversion of the cellular isoform (PrPC) to PrPSc is accompanied by a diminution in the alpha-helical content and an increase in the beta-sheet structure. To investigate the structural basis of this transition, peptide ...

journal_title：Journal of molecular biology

authors： Zhang H,Kaneko K,Nguyen JT,Livshits TL,Baldwin MA,Cohen FE,James TL,Prusiner SB

更新日期：1995-07-21 00:00:00

abstract：:Three fundamentally different chloroplast ATP synthase samples of increasing complexity were visualized by atomic force microscopy. The samples are distinguishable in respect to the isolation technique, the detergent employed, and the final subunit composition. The homo-oligomer III was isolated following SDS treatmen...

journal_title：Journal of molecular biology

authors： Seelert H,Dencher NA,Müller DJ

更新日期：2003-10-17 00:00:00

abstract：:The roll-twist-slide correlation in the DNA crystal structures that are collected in the Nucleic Acid Data Base is analyzed in order to obtain a general understanding of the effects of the nucleotide sequence on the 3D structure of a dinucleotide step. It is concluded that the differences between the pyrimidine bases ...

journal_title：Journal of molecular biology

authors： Suzuki M,Amano N,Kakinuma J,Tateno M

更新日期：1997-12-05 00:00:00

abstract：:Six mutants of Escherichia coli K12 that are sensitive to visible light have been isolated. Five of them, including an amber mutant, are defective in a gene that maps near 11 minutes on the linkage map of the chromosome, and this gene has been designated visA. The sixth mutant, which was isolated from bacteria that ca...

journal_title：Journal of molecular biology

authors： Miyamoto K,Nakahigashi K,Nishimura K,Inokuchi H

更新日期：1991-06-05 00:00:00

abstract：:The infrared dichroic ratios of the amide bands from oriented beta-barrels yield an experimental value for the mean orientation, beta, of the beta-strands, relative to the barrel axis. For a barrel of n strands, this then gives the shear number, S, that characterizes the stagger of the beta-sheet. Combining values of ...

journal_title：Journal of molecular biology

authors： Marsh D

更新日期：2000-03-31 00:00:00

abstract：:Drugs can affect function in proteins by modulating their flexibility. Despite this possibility, there are very few studies on how drug binding affects the dynamics of target macromolecules. FKBP12 (FK506 binding protein 12) is a prolyl cis-trans isomerase and a drug target. The immunosuppressant drug rapamycin exerts...

journal_title：Journal of molecular biology

authors： Sapienza PJ,Mauldin RV,Lee AL

更新日期：2011-01-14 00:00:00

abstract：:The role of an 18-residue ion-pair network, that is present in the glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus, in conferring stability to other, less stable homologous enzymes, has been studied by introducing four new charged amino acid residues into the subunit interface of glutam...

journal_title：Journal of molecular biology

authors： Lebbink JH,Knapp S,van der Oost J,Rice D,Ladenstein R,de Vos WM

更新日期：1999-06-04 00:00:00

abstract：:Oncogenes may play a crucial role in the genetic program of cellular differentiation; even, probably, at a very early stage in this program, which can be described as pre-commitment. We have investigated the variation in, and the control level of, the accumulation of the transformation-associated cellular protein p53 ...

journal_title：Journal of molecular biology

authors： Khochbin S,Principaud E,Chabanas A,Lawrence JJ

更新日期：1988-03-05 00:00:00

abstract：:We have examined the chromatin organization of a cluster of transfer RNA genes at the cytogenic locus 90BC on the right arm of the third Drosophila melanogaster chromosome. We find that the internal promoter sequences are preferentially exposed to micrococcal nuclease and DNase I in chromatin. Moreover, these exposed ...

journal_title：Journal of molecular biology

authors： DeLotto R,Schedl P

更新日期：1984-11-15 00:00:00

abstract：:We report the preparation, crystallization and preliminary X-ray crystallographic study of the Fab fragment from a heteroclitic murine (BALB/c) monoclonal anti-hen egg-white lysozyme antibody complexed with a heterologous antigen, pheasant lysozyme. The complex between the heterologous antigen and the antibody has bee...

journal_title：Journal of molecular biology

authors： Guillon V,Alzari PM,Poljak RJ

更新日期：1987-09-20 00:00:00

abstract：:Polar residues are capable of mediating the association of membrane-embedded helices through the formation of side-chain/side-chain inter-helical hydrogen bonds. However, the extent to which native van der Waals packing of the residues surrounding the polar locus can enhance, or interfere with, the interaction of pola...

journal_title：Journal of molecular biology

authors： Dawson JP,Melnyk RA,Deber CM,Engelman DM

更新日期：2003-08-01 00:00:00

abstract：:The T7 RNA polymerase promoter has been proposed to contain two domains: the binding region upstream of position -5 is recognized through apparently traditional duplex contacts, while the catalytic domain downstream of position -5 is bound in a melted configuration. This model is tested by following polymerase binding...

journal_title：Journal of molecular biology

authors： Ujvári A,Martin CT

更新日期：1997-11-07 00:00:00

abstract：:The junction-resolving enzymes are a class of nucleases that introduce paired cleavages into four-way DNA junctions. They are important in DNA recombination and repair, and are found throughout nature, from eubacteria and their bacteriophages through to higher eukaryotes and their viruses. These enzymes exhibit struct...

journal_title：Journal of molecular biology

authors： White MF,Giraud-Panis MJ,Pöhler JR,Lilley DM

更新日期：1997-06-27 00:00:00

abstract：:The activation and latency of human immunodeficiency virus type 1 (HIV-1) are tightly controlled by the transcriptional activity of its long terminal repeat (LTR) region. The LTR is regulated by viral proteins as well as host factors, including the nuclear factor kappaB (NF-kappaB) that becomes activated in virus-infe...

journal_title：Journal of molecular biology

authors： Stroud JC,Oltman A,Han A,Bates DL,Chen L

更新日期：2009-10-16 00:00:00