Crystallization of a DNA duplex 15-mer containing unpaired bases: d(CGCGAAATTTACGCG).

Abstract:

:Crystals of an almost self-complementary DNA 15-mer d(CGCGAAATTTACGCG) have been grown by the vapor diffusion technique at 4 degrees C. The space group is I222 with a = 37.3 A, b = 54.6 A and c = 104.8 A. Solution studies showed that the 15-mer forms a duplex with the extra adenine residue unpaired: (sequence; see text) Crystals are stable at 4 degrees C and are suitable for medium-resolution structural studies.

journal_name

J Mol Biol

authors

Miller M,Wlodawer A,Appella E,Sussman JL

doi

10.1016/0022-2836(87)90503-1

subject

Has Abstract

pub_date

1987-06-20 00:00:00

pages

967-8

issue

4

eissn

0022-2836

issn

1089-8638

pii

0022-2836(87)90503-1

journal_volume

195

pub_type

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