Phenotypic suppression by incorporation of an alien amino acid.

abstract：:The crystal structure of muconate lactonizing enzyme has been solved at 3 A resolution, and an unambiguous alpha-carbon backbone chain trace made. The enzyme contains three domains; the central domain is a parallel-stranded alpha-beta barrel, which has previously been reported in six other enzymes, including triose ph...

journal_title：Journal of molecular biology

authors： Goldman A,Ollis DL,Steitz TA

更新日期：1987-03-05 00:00:00

abstract：:The Psi-RNA packaging signal of the human immunodeficiency virus type-1 (HIV-1) genome contains a 35 nucleotide stem-loop, termed SL1, which is important for efficient genome packaging during virus assembly and for reverse transcription during infectivity. The predicted secondary structure of SL1 consists of an upper ...

journal_title：Journal of molecular biology

authors： Lawrence DC,Stover CC,Noznitsky J,Wu Z,Summers MF

更新日期：2003-02-14 00:00:00

abstract：:The ribosomal RNA multigene family in the fungus fly Sciara coprophila contains a total of only 65 to 70 repeat units. We explored the types and frequencies of variant repeats in this small multigene family by characterizing different cloned rDNA variants from Sciara. Although we did not observe any intergenic spacer ...

journal_title：Journal of molecular biology

authors： Kerrebrock AW,Srivastava R,Gerbi SA

更新日期：1989-11-05 00:00:00

abstract：:The average structural and dynamic properties of tetragonal hen egg-white lysozyme have been compared, in structures refined at 1.9 A resolution, using data collected at 100 K and 298 K. The molecule expands by 1.8% over this temperature range with the expansion occurring primarily in its small sub-atomic-sized spaces...

journal_title：Journal of molecular biology

authors： Young AC,Tilton RF,Dewan JC

更新日期：1994-01-07 00:00:00

abstract：:The 1.9 A structure of the C-terminal dehydrogenase part of the rat peroxisomal monomeric multifunctional enzyme type 1 (MFE-1) has been determined. In this construct (residues 260-722 and referred to as MFE1-DH) the N-terminal hydratase part of MFE-1 has been deleted. The structure of MFE1-DH shows that it consists o...

journal_title：Journal of molecular biology

authors： Taskinen JP,Kiema TR,Hiltunen JK,Wierenga RK

更新日期：2006-01-27 00:00:00

abstract：:Genetic circuits, composed of complex networks of interacting molecular machines, enable living systems to sense their dynamic environments, perform computation on the inputs, and formulate appropriate outputs. By rewiring and expanding these circuits with novel parts and modules, synthetic biologists have adapted liv...

journal_title：Journal of molecular biology

authors： Ma KC,Perli SD,Lu TK

更新日期：2016-02-27 00:00:00

abstract：:Using homopolymeric units of either phenylalanine or tryptophan to replace the natural core segment of the Escherichia coli alkaline phosphatase signal peptide, the hydrophobicity requirements for protein export and processing were further explored. The mutant signal peptide containing polyphenylalanine functioned at ...

journal_title：Journal of molecular biology

authors： Rusch SL,Kendall DA

更新日期：1992-03-05 00:00:00

abstract：:An analysis was performed on 335 pairs of structurally aligned proteins derived from the structural classification of proteins (SCOP http://scop.mrc-lmb.cam.ac.uk/scop/) database. These similarities were divided into analogues, defined as proteins with similar three-dimensional structures (same SCOP fold classificatio...

journal_title：Journal of molecular biology

authors： Russell RB,Saqi MA,Sayle RA,Bates PA,Sternberg MJ

更新日期：1997-06-13 00:00:00

abstract：:Isoniazid (INH) was the first synthesized drug that mediated bactericidal killing of the bacterium Mycobacterium tuberculosis, a major clinical breakthrough. To this day, INH remains a cornerstone of modern tuberculosis (TB) chemotherapy. This review describes the serendipitous discovery of INH, its effectiveness on T...

journal_title：Journal of molecular biology

authors： Vilchèze C,Jacobs WR Jr

更新日期：2019-08-23 00:00:00

abstract：:Titin is a giant, multidomain muscle protein forming a major component of the sarcomere in vertebrate striated muscle. As for many other multidomain proteins, the properties of titin are often studied by characterisation of the constituent domains in isolation. This raises the question of to what extent the properties...

journal_title：Journal of molecular biology

authors： Scott KA,Steward A,Fowler SB,Clarke J

更新日期：2002-01-25 00:00:00

abstract：:Enhanced structural insights into the folding energy landscape of the N-terminal dimerization domain of Escherichia coli tryptophan repressor, [2-66]2 TR, were obtained from a combined experimental and theoretical analysis of its equilibrium folding reaction. Previous studies have shown that the three intertwined heli...

journal_title：Journal of molecular biology

authors： Simler BR,Levy Y,Onuchic JN,Matthews CR

更新日期：2006-10-13 00:00:00

abstract：:The int gene of phage lambda encodes a protein involved in site-specific recombination. Its expression is regulated differentially during successive phases of the lambda infective cycle. The gene is transcribed early after infection from one promoter, pL, and later from a second promoter pI. Each transcription event r...

journal_title：Journal of molecular biology

authors： Schmeissner U,McKenney K,Rosenberg M,Court D

更新日期：1984-06-15 00:00:00

abstract：:Carbohydrate-binding polypeptides, including carbohydrate-binding modules (CBMs) from polysaccharidases, and lectins, are widespread in nature. Whilst CBMs are classically considered distinct from lectins, in that they are found appended to polysaccharide-degrading enzymes, this distinction is blurring. The crystal st...

journal_title：Journal of molecular biology

authors： Boraston AB,Notenboom V,Warren RA,Kilburn DG,Rose DR,Davies G

更新日期：2003-03-28 00:00:00

abstract：:To determine the conformational requirements for antibody recognition and extent of flexibility within a protein epitope, a chimeric influenza A virus neuraminidase (NA) has been constructed in which five discontinuous polypeptide segments from a subtype N9 NA, which comprise the monoclonal antibody NC41 epitope, have...

journal_title：Journal of molecular biology

authors： Nuss JM,Air GM

更新日期：1994-01-14 00:00:00

abstract：:The GTPase Center (GAC) RNA domain in bacterial 23S rRNA is directly bound by ribosomal protein L11, and this complex is essential to ribosome function. Previous cocrystal structures of the 58-nucleotide GAC RNA bound to L11 revealed the intricate tertiary fold of the RNA domain, with one monovalent and several divale...

journal_title：Journal of molecular biology

authors： Welty R,Rau M,Pabit S,Dunstan MS,Conn GL,Pollack L,Hall KB

更新日期：2020-02-14 00:00:00

abstract：:Crystals of NADPH:FMN oxidoreductase from Vibrio harveyi have been obtained and characterized by X-ray diffraction. This enzyme plays a role in the generation of light in luminescent bacteria by providing reduced FMN to luciferase. Large, high quality crystals were grown using polyethylene glycol 6000 at pH 7.0. They ...

journal_title：Journal of molecular biology

authors： Tanner J,Lei B,Liu M,Tu SC,Krause KL

更新日期：1994-08-12 00:00:00

abstract：:Three crystal forms of canine myeloperoxidase are described. An orthorhombic form in space group P2(1)2(1)2(1) has unit cell dimensions: a = 108.3 A (1 A = 0.1 nm) b = 205.9 A and c = 139.9 A. A trigonal form in space group P3(1)21 or P3(2)21 has unit cell dimensions: a = b = 138.9 A and c = 145.2 A. A monoclinic form...

journal_title：Journal of molecular biology

authors： Fenna RE

更新日期：1987-08-20 00:00:00

abstract：:The chloroplast thylakoid membrane of green plants is organized in stacked grana membranes and unstacked stroma membranes. We investigated the structural organization of Photosystem II (PSII) in paired grana membrane fragments by transmission electron microscopy. The membrane fragments were obtained by a short treatme...

journal_title：Journal of molecular biology

authors： Boekema EJ,van Breemen JF,van Roon H,Dekker JP

更新日期：2000-09-01 00:00:00

abstract：:We have identified the recognition sequence for the Citrobacter freundii restriction endonuclease CfrA, a member of the A-family of type I R-M enzymes. This bipartite target sequence differs in both its components from those of other type I enzymes. We determined the nucleotide sequence of its specificity gene (hsdS) ...

journal_title：Journal of molecular biology

authors： Kannan P,Cowan GM,Daniel AS,Gann AA,Murray NE

更新日期：1989-10-05 00:00:00

abstract：:In vitro packaging of bacteriophage SPP1 DNA into procapsids is described and the requirements of this process were determined. Combination of proheads with an extract supplying terminase, DNA and phage tails yielded up to 10(7 )viable phages per milliliter of in vitro reaction under optimized conditions. The presence...

journal_title：Journal of molecular biology

authors： Dröge A,Tavares P

更新日期：2000-02-11 00:00:00

abstract：:The nucleotide sequences of nine genes corresponding to tRNA(Ser)4 or tRNA(Ser)7 of Drosophila melanogaster were determined. Eight of the genes compose the major tRNA(Ser)4,7 cluster at 12DE on the X chromosome, while the other is from 23E on the left arm of chromosome 2. Among the eight X-linked genes, five different...

journal_title：Journal of molecular biology

authors： Cribbs DL,Leung J,Newton CH,Hayashi S,Miller RC Jr,Tener GM

更新日期：1987-10-05 00:00:00

abstract：:To quantify the relationship between sequence and transmembrane dimer stability, a systematic mutagenesis and thermodynamic study of the protein-protein interaction residues in the glycophorin A transmembrane helix-helix dimer was carried out. The results demonstrate that the glycophorin A transmembrane sequence dimer...

journal_title：Journal of molecular biology

authors： Doura AK,Kobus FJ,Dubrovsky L,Hibbard E,Fleming KG

更新日期：2004-08-20 00:00:00

abstract：:Escherichia coli UvrD is a superfamily 1 helicase/translocase involved in multiple DNA metabolic processes including methyl-directed mismatch DNA repair. Although a UvrD monomer can translocate along single-stranded DNA, a UvrD dimer is needed for processive helicase activity in vitro. E. coli MutL, a regulatory prote...

journal_title：Journal of molecular biology

authors： Ordabayev YA,Nguyen B,Niedziela-Majka A,Lohman TM

更新日期：2018-10-19 00:00:00

abstract：:Dihydropyrimidinases (hydantoinases) catalyse the reversible hydrolytic ring-opening of cyclic diamides such as dihydropyrimidines in the catabolism of pyrimidines. In biotechnology, these enzymes find application in the enantiospecific production of amino acids from racemic hydantoins. The crystal structure of a D-en...

journal_title：Journal of molecular biology

authors： Abendroth J,Niefind K,Schomburg D

更新日期：2002-06-28 00:00:00

abstract：:We have used lacZ reporter genes to assess leftward ribosome frameshifting on sequences containing the quadruplet U UUC followed by several different triplets coding for lysine, isoleucine, or leucine. Limitation for lysine-tRNA provokes leftward frameshifting when the slippery quadruplet is followed by either lysine ...

journal_title：Journal of molecular biology

authors： Barak Z,Lindsley D,Gallant J

更新日期：1996-03-08 00:00:00

abstract：:The A-isozyme of O-acetylserine sulfhydrylase, a pyridoxal phosphate-dependent enzyme isolated from Salmonella typhimurium catalyzes the synthesis of L-cysteine from O-acetyl-L-serine and sulfide. The pyridoxal form of the enzyme has been crystallized in two different forms. One form is in the orthorhombic space group...

journal_title：Journal of molecular biology

authors： Rao GS,Mottonen J,Goldsmith EJ,Cook PF

更新日期：1993-06-20 00:00:00

abstract：:Adenylate and guanylate cyclases, having different but related substrates, are a paradigm for the study of substrate discrimination. A prokaryotic adenylate cyclase gene, phylogenetically related to eukaryotic counterparts, was screened for mutants remodelling the enzyme's specificity. In a first step, a mutant was se...

journal_title：Journal of molecular biology

authors： Beuve A,Danchin A

更新日期：1992-06-20 00:00:00

abstract：:Methyltransferases from the m(1)A(58) tRNA methyltransferase (TrmI) family catalyze the S-adenosyl-l-methionine-dependent N(1)-methylation of tRNA adenosine 58. The crystal structure of Thermus thermophilus TrmI, in complex with S-adenosyl-l-homocysteine, was determined at 1.7 A resolution. This structure is closely r...

journal_title：Journal of molecular biology

authors： Barraud P,Golinelli-Pimpaneau B,Atmanene C,Sanglier S,Van Dorsselaer A,Droogmans L,Dardel F,Tisné C

更新日期：2008-03-21 00:00:00

abstract：:An essential step of pre-mRNA spliceosome assembly is the interaction between the snRNPs U4/U6 and U5, to form the [U4/U6.U5] tri-snRNP. While the tri-snRNP protein Prp6p appears to play an important role for tri-snRNP formation in yeast, little is known about the interactions that connect the two snRNP particles in h...

journal_title：Journal of molecular biology

authors： Makarov EM,Makarova OV,Achsel T,Lührmann R

更新日期：2000-05-12 00:00:00

abstract：:The initiator of coliphage lambda DNA replication, lambda O protein, may be detected among other 35S-labeled phage and bacterial proteins by a method based on immunoprecipitation. This method makes it possible to study lambda O proteolytic degradation in lambda plasmid-harboring or lambda phage-infected cells; it avoi...

journal_title：Journal of molecular biology

authors： Wegrzyn G,Pawlowicz A,Taylor K

更新日期：1992-08-05 00:00:00