A GAF domain in the hypoxia/NO-inducible Mycobacterium tuberculosis DosS protein binds haem.

abstract：:DNA structures bound to transcription factors are studied by using the crystal coordinates of complexes. Structural characteristics which are found at the sites where a protein secondary structure, an alpha-helix or a beta-sheet binds, can be understood in terms of fitting of the concave surface of the DNA major groov...

journal_title：Journal of molecular biology

authors： Suzuki M,Yagi N

更新日期：1996-02-09 00:00:00

abstract：:Botulism is caused by botulinum neurotoxin (BoNT), the most poisonous substance known. Potential use of BoNT as a biothreat agent has made development of sensitive assays for toxin detection and potent antitoxin for treatment of intoxication a high priority. To improve detection and treatment of botulism, molecular ev...

journal_title：Journal of molecular biology

authors： Razai A,Garcia-Rodriguez C,Lou J,Geren IN,Forsyth CM,Robles Y,Tsai R,Smith TJ,Smith LA,Siegel RW,Feldhaus M,Marks JD

更新日期：2005-08-05 00:00:00

abstract：:A complex pseudoknot structure surrounds the first ribosome initiation site in the Escherichia coli alpha mRNA and mediates its regulation by ribosomal protein S4. A 112 nt RNA fragment containing this pseudoknot exists in two conformations that are resolvable by gel electrophoresis below room temperature. Between 30 ...

journal_title：Journal of molecular biology

authors： Gluick TC,Gerstner RB,Draper DE

更新日期：1997-07-18 00:00:00

abstract：:The structure and activities of the recombination-promoting P22 Erf protein were examined in vitro. Treatment of the protein with elastase produces a stable amino-terminal fragment, consisting of amino acid residues 1 to (approximately) 136. We have purified this fragment, designated fragment B, to apparent homogeneit...

journal_title：Journal of molecular biology

authors： Poteete AR,Sauer RT,Hendrix RW

更新日期：1983-12-25 00:00:00

abstract：:Abasic lesions are common mutagenic or cytotoxic DNA damages. Ape1 is the major human apurinic/apyrimidinic (AP) endonuclease and initiates repair of abasic sites by catalyzing strand cleavage at the lesion. I show here that Ape1 single-stranded (ss) AP site incision activity prefers 0.5 mM or 2 mM MgCl(2) and low con...

journal_title：Journal of molecular biology

authors： Wilson DM 3rd

更新日期：2005-02-04 00:00:00

abstract：:Atomic contact potentials are derived by statistical analysis of atomic surface contact areas versus atom type in a database of non-homologous protein structures. The atomic environment is characterized by the surface area accessible to solvent and the surface of contacts with polar and non-polar atoms. Four types of ...

journal_title：Journal of molecular biology

authors： Delarue M,Koehl P

更新日期：1995-06-09 00:00:00

abstract：:Herpesvirus proteases are essential for the production of progeny virus. They cleave the assembly protein that fills the immature capsid in order to make place for the viral DNA. The recombinant protease of the human gamma-herpesvirus Epstein-Barr virus (EBV) was expressed in Escherichia coli and purified. Circular di...

journal_title：Journal of molecular biology

authors： Buisson M,Valette E,Hernandez JF,Baudin F,Ebel C,Morand P,Seigneurin JM,Arlaud GJ,Ruigrok RW

更新日期：2001-08-03 00:00:00

abstract：:The secondary structure of ribonuclease T1 (RNase T1) in aqueous solution and its temperature-induced structural changes have been investigated by Fourier-transform infrared (FT-IR) spectroscopy. 13 to 14% alpha-helix and 32 to 33% beta-sheet were estimated from the resolution-enhanced FT-IR spectra, in agreement with...

journal_title：Journal of molecular biology

authors： Fabian H,Schultz C,Naumann D,Landt O,Hahn U,Saenger W

更新日期：1993-08-05 00:00:00

abstract：:In all living cells, regulated passage across membranes of specific proteins occurs through a universally conserved secretory channel. In bacteria and chloroplasts, the energy for the mechanical work of moving polypeptides through that channel is provided by SecA, a regulated ATPase. Here, we use site-directed spin la...

journal_title：Journal of molecular biology

authors： Cooper DB,Smith VF,Crane JM,Roth HC,Lilly AA,Randall LL

更新日期：2008-09-26 00:00:00

abstract：:The crystal structure of muconate lactonizing enzyme has been solved at 3 A resolution, and an unambiguous alpha-carbon backbone chain trace made. The enzyme contains three domains; the central domain is a parallel-stranded alpha-beta barrel, which has previously been reported in six other enzymes, including triose ph...

journal_title：Journal of molecular biology

authors： Goldman A,Ollis DL,Steitz TA

更新日期：1987-03-05 00:00:00

abstract：:Experimental and theoretical thermodynamic studies of the consequences of 2'-hydroxyl substitution in the RNA UUCG tetraloop show distinct position dependence consistent with the diverse structural contexts of the four-loop ribose hydroxyls in this motif. The results suggest that even for simple substitutions, such as...

journal_title：Journal of molecular biology

authors： Williams DJ,Hall KB

更新日期：2000-03-17 00:00:00

abstract：:Interactions of transmembrane helices play an important role in folding and oligomerization of integral membrane proteins. The interfacial residues of these helices frequently correspond to heptad repeat motifs. In order to uncover novel mechanisms underlying these interactions, we randomised a heptad repeat pattern w...

journal_title：Journal of molecular biology

authors： Ridder A,Skupjen P,Unterreitmeier S,Langosch D

更新日期：2005-12-09 00:00:00

abstract：:The tyrosine residues adjacent to the C termini of the hemoglobin (Hb) subunits, alphaY140 and betaY145, are expected to play important structural roles, because the C termini are the loci of T-state quaternary salt-bridges, and because the tyrosine side-chains bridge the H and F helices via H bonds to the alphaV93 an...

journal_title：Journal of molecular biology

authors： Kneipp J,Balakrishnan G,Chen R,Shen TJ,Sahu SC,Ho NT,Giovannelli JL,Simplaceanu V,Ho C,Spiro TG

更新日期：2006-02-17 00:00:00

abstract：:Expression of the cellular prion protein is necessary for the transmission and propagation of prion diseases. Increasing the level of prion protein expression decreases the incubation period for these diseases. Therefore, understanding the regulation of prion protein expression could be critical for treating or preven...

journal_title：Journal of molecular biology

authors： Haigh CL,Wright JA,Brown DR

更新日期：2007-05-11 00:00:00

abstract：:Molten globules are partially structured protein folding intermediates that adopt a native-like overall backbone topology in the absence of extensive detectable tertiary interactions. It is important to determine the extent of specific tertiary structure present in molten globules and to understand the role of specifi...

journal_title：Journal of molecular biology

authors： Wu LC,Kim PS

更新日期：1998-07-03 00:00:00

abstract：:Spider dragline silk, one of the strongest polymers in nature, is composed of proteins termed major ampullate spidroin (MaSp) 1 and MaSp2. The N-terminal (NT) domain of MaSp1 produced by the nursery web spider Euprosthenops australis acts as a pH-sensitive relay, mediating spidroin assembly at around pH 6.3. Using ami...

journal_title：Journal of molecular biology

authors： Landreh M,Askarieh G,Nordling K,Hedhammar M,Rising A,Casals C,Astorga-Wells J,Alvelius G,Knight SD,Johansson J,Jörnvall H,Bergman T

更新日期：2010-11-26 00:00:00

abstract：:The initiator of coliphage lambda DNA replication, lambda O protein, may be detected among other 35S-labeled phage and bacterial proteins by a method based on immunoprecipitation. This method makes it possible to study lambda O proteolytic degradation in lambda plasmid-harboring or lambda phage-infected cells; it avoi...

journal_title：Journal of molecular biology

authors： Wegrzyn G,Pawlowicz A,Taylor K

更新日期：1992-08-05 00:00:00

abstract：:Sir2 is a protein deacetylase that mediates transcriptional silencing at the HM loci, telomeres, and rDNA repeats in yeast. To identify functionally significant regions of the Sir2 protein, we have characterized two types of mutations. First, we used random mutagenesis to create temperature-sensitive alleles of the SI...

journal_title：Journal of molecular biology

authors： Hickman M,McCullough K,Woike A,Raducha-Grace L,Rozario T,Dula ML,Anderson E,Margalit D,Holmes SG

更新日期：2007-04-13 00:00:00

abstract：:Microbial organisms utilize light not only as energy sources but also as signals by which rhodopsins (containing retinal as a chromophore) work as photoreceptors. Sensory rhodopsin I (SRI) is a dual photoreceptor that regulates both negative and positive phototaxis in microbial organisms, such as the archaeon Halobact...

journal_title：Journal of molecular biology

authors： Suzuki D,Furutani Y,Inoue K,Kikukawa T,Sakai M,Fujii M,Kandori H,Homma M,Sudo Y

更新日期：2009-09-11 00:00:00

abstract：:Antimycin A (antimycin), one of the first known and most potent inhibitors of the mitochondrial respiratory chain, binds to the quinone reduction site of the cytochrome bc1 complex. Structure-activity relationship studies have shown that the N-formylamino-salicyl-amide group is responsible for most of the binding spec...

journal_title：Journal of molecular biology

authors： Huang LS,Cobessi D,Tung EY,Berry EA

更新日期：2005-08-19 00:00:00

abstract：:The mechanism by which the binuclear metallophosphotriesterases (PTEs, E.C. 3.1.8.1) catalyse substrate hydrolysis has been extensively studied. The mu-hydroxo bridge between the metal ions has been proposed to be the initiating nucleophile in the hydrolytic reaction. In contrast, analysis of some biomimetic systems h...

journal_title：Journal of molecular biology

authors： Jackson CJ,Foo JL,Kim HK,Carr PD,Liu JW,Salem G,Ollis DL

更新日期：2008-02-01 00:00:00

abstract：:The negative regulatory domain (NRD) of the p53 tumor suppressor is intrinsically disordered. It contains several posttranslational modification (PTM) sites that are important for regulation of p53 activity. Calcium-dependent binding of dimeric S100B(ββ) to p53-NRD blocks access to these PTM sites and disrupts the p53...

journal_title：Journal of molecular biology

authors： McDowell C,Chen J,Chen J

更新日期：2013-03-25 00:00:00

abstract：:We have isolated and sequenced a cloned complementary DNA insert complementary to the messenger RNA of a cytoplasmic actin expressed in human epidermal cells. This provides the first cytoplasmic actin complementary DNA sequence for a vertebrate organism. The actin amino acid sequence predicted from this complementary ...

journal_title：Journal of molecular biology

authors： Hanukoglu I,Tanese N,Fuchs E

更新日期：1983-02-05 00:00:00

abstract：:The structure of Mn(III) superoxide dismutase (Mn(III)SOD) from Thermus thermophilus, a tetramer of chains 203 residues in length, has been refined by restrained least-squares methods. The R-factor [formula: see text] for the 54,056 unique reflections measured between 10.0 and 1.8 A (96% of all possible reflections) i...

journal_title：Journal of molecular biology

authors： Ludwig ML,Metzger AL,Pattridge KA,Stallings WC

更新日期：1991-05-20 00:00:00

abstract：:The interactions between the complement receptor type 2 (CR2) and the C3 complement fragments C3d, C3dg, and iC3b are essential for the initiation of a normal immune response. A crystal-derived structure of the two N-terminal short consensus repeat (SCR1-2) domains of CR2 in complex with C3d has previously been elucid...

journal_title：Journal of molecular biology

authors： Shaw CD,Storek MJ,Young KA,Kovacs JM,Thurman JM,Holers VM,Hannan JP

更新日期：2010-12-10 00:00:00

abstract：:We present evidence that the transcription termination factor Rho is autogenously regulated in Escherichia coli. The steady-state level of Rho is increased approximately tenfold in rho mutant cells. In the rho+ revertants, the content of Rho is similar to the wild-type level. A rho-/rho+ merodiploid produces equimolar...

journal_title：Journal of molecular biology

authors： Barik S,Bhattacharya P,Das A

更新日期：1985-04-20 00:00:00

abstract：:Type IV pili are bacterial extracellular filaments that can be retracted to create force and motility. Retraction is accomplished by the motor protein PilT. Crystal structures of Pseudomonas aeruginosa PilT with and without bound beta,gamma-methyleneadenosine-5'-triphosphate have been solved at 2.6 A and 3.1 A resolut...

journal_title：Journal of molecular biology

authors： Misic AM,Satyshur KA,Forest KT

更新日期：2010-07-30 00:00:00

abstract：:DNA replication fork arrest during the termination phase of chromosome replication in Bacillus subtilis is brought about by the replication terminator protein (RTP) bound to specific DNA terminator sequences (Ter sites) distributed throughout the terminus region. An attractive suggestion by others was that crucial to ...

journal_title：Journal of molecular biology

authors： Duggin IG,Andersen PA,Smith MT,Wilce JA,King GF,Wake RG

更新日期：1999-03-12 00:00:00

abstract：:DNA gyrase negatively supercoils DNA in a reaction coupled to the binding and hydrolysis of ATP. Limited supercoiling can be achieved in the presence of the non-hydrolysable ATP analogue, 5'-adenylyl beta,gamma-imidodiphosphate (ADPNP). In order to negatively supercoil DNA, gyrase must wrap a length of DNA around itse...

journal_title：Journal of molecular biology

authors： Heddle JG,Mitelheiser S,Maxwell A,Thomson NH

更新日期：2004-03-26 00:00:00

abstract：:We have developed a formalism and a computational method for analyzing the potential functional consequences of non-synonymous single nucleotide polymorphisms. Our approach uses a structural model and phylogenetic information to derive a selection of structure and sequence-based features serving as indicators of an am...

journal_title：Journal of molecular biology

authors： Chasman D,Adams RM

更新日期：2001-03-23 00:00:00