当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Cloning and characterization of a novel Bacillus thuringiensis cytolytic delta-endotoxin.

Cloning and characterization of a novel Bacillus thuringiensis cytolytic delta-endotoxin.

Abstract：

:A gene encoding a major entomocidal polypeptide from Bacillus thuringiensis subspecies kyushuensis delta-endotoxin crystals (CytB) was cloned into Escherichia coli and sequenced. The deduced amino acid sequence gave a predicted molecular mass of 29,236 Da and showed 39% identity and 70% similarity with the 27,371 Da CytA protein from Bacillus thuringiensis subspecies israelensis. The larger size of CytB compared to CytA appears to be due to additional sequence in CytB after the CytA C-terminus. Unlike CytA, CytB was freely expressed in Escherichia coli and formed cytoplasmic inclusions without the need for a "helper" protein. Electron microscopic observation of CytB inclusions revealed them to be generally amorphous, but examples possessing some lattice structure were seen.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Koni PA,Ellar DJ

doi

10.1006/jmbi.1993.1037

subject

Has Abstract

pub_date

1993-01-20 00:00:00

pages

319-27

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)71037-5

journal_volume

229

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Enhancing the contrast of ApoB to locate the surface components in the 3D density map of human LDL.

    abstract：:A 26 Å resolution map of the structure of human low-density lipoprotein (LDL) was obtained from electron cryomicroscopy and single-particle image reconstruction. The structure showed a discoidal-shaped LDL particle with high-density regions mainly distributed at the edge of the particle and low-density regions at the ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.10.034

    authors： Liu Y,Atkinson D

    更新日期：2011-01-07 00:00:00

  • Coliphage P2 late control gene ogr. DNA sequence and product identification.

    abstract：:The bacteriophage P2 late control gene ogr was cloned and precisely localized by deletion analysis in vitro. The DNA sequence of the ogr gene containing the ogr1 mutation was determined. The sequence translates into a basic protein of a molecular weight of 8300. Plasmids overproducing the ogr gene product were constru...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(86)90170-1

    authors： Birkeland NK,Lindquist BH

    更新日期：1986-04-05 00:00:00

  • Crystal structure of an RNA duplex r(gugucgcac)(2) with uridine bulges.

    abstract：:The crystal structure of a nonamer RNA duplex with a uridine bulge in each strand, r(gugucgcac)(2), was determined at 1.4 A resolution. The structure was solved by multiple anomalous diffraction phasing method using a three-wavelength data set collected at the Advanced Protein Source and refined to a final R(work)/R(f...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5045

    authors： Xiong Y,Deng J,Sudarsanakumar C,Sundaralingam M

    更新日期：2001-10-26 00:00:00

  • Nucleotide-Free sB-Raf is Preferentially Bound by Hsp90 and Cdc37 In Vitro.

    abstract：:The molecular chaperone Hsp90 and its cofactor Cdc37 are required for the stability of protein kinases in the cellular environment. Upon pharmacological inhibition of Hsp90, the Hsp90-dependent kinases are degraded quickly by the proteasome. Clear physiological evidence for the formation of heterooligomeric complexes ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2016.09.002

    authors： Eckl JM,Daake M,Schwartz S,Richter K

    更新日期：2016-10-09 00:00:00

  • The importance of G.A hydrogen bonding in the metal ion- and protein-induced folding of a kink turn RNA.

    abstract：:The kink turn (K-turn) is a common motif in RNA structure, found in many RNA species important in translation, RNA modification and splicing, and the control of gene expression. In general the K-turn comprises a three nucleotide bulge followed by trans sugar-Hoogsteen G.A pairs. The RNA adopts a tightly kinked conform...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.05.052

    authors： Turner B,Lilley DM

    更新日期：2008-08-29 00:00:00

  • Crystal structure and comparative functional analyses of a Mycobacterium aldo-keto reductase.

    abstract：:Aldo-keto reductases (AKRs) are a large superfamily of NADPH-dependent enzymes that catalyze the reduction of aldehydes, aldoses, dicarbonyls, steroids, and monosaccharides. While their precise physiological role is generally unknown, AKRs are nevertheless involved in the detoxification of a broad range of toxic metab...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.02.021

    authors： Scoble J,McAlister AD,Fulton Z,Troy S,Byres E,Vivian JP,Brammananth R,Wilce MC,Le Nours J,Zaker-Tabrizi L,Coppel RL,Crellin PK,Rossjohn J,Beddoe T

    更新日期：2010-04-23 00:00:00

  • Effects of the nucleoid protein HU on the structure, flexibility, and ring-closure properties of DNA deduced from Monte Carlo simulations.

    abstract：:The histone-like HU (heat unstable) protein plays a key role in the organization and regulation of the Escherichia coli genome. The nonspecific nature of HU binding to DNA complicates analysis of the mechanism by which the protein contributes to the looping of DNA. Conventional models of the looping of HU-bound duplex...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.05.088

    authors： Czapla L,Swigon D,Olson WK

    更新日期：2008-10-03 00:00:00

  • Modeling a minimal ribosome based on comparative sequence analysis.

    abstract：:We have determined the three-dimensional organization of ribosomal RNAs and proteins essential for minimal ribosome function. Comparative sequence analysis identifies regions of the ribosome that have been evolutionarily conserved, and the spatial organization of conserved domains is determined by mapping these onto s...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00568-5

    authors： Mears JA,Cannone JJ,Stagg SM,Gutell RR,Agrawal RK,Harvey SC

    更新日期：2002-08-09 00:00:00

  • Nuclear magnetic resonance solution structure of dendrotoxin K from the venom of Dendroaspis polylepis polylepis.

    abstract：:The solution structure of dendrotoxin K (Toxin K), a protein consisting of one polypeptide chain with 57 residues purified from the venom of the black mamba, Dendroaspis polylepis polylepis, was determined by nuclear magnetic resonance (NMR) spectroscopy. On the basis of virtually complete sequence-specific 1H NMR ass...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1623

    authors： Berndt KD,Güntert P,Wüthrich K

    更新日期：1993-12-05 00:00:00

  • Mapping of the docking of SecA onto the chaperone SecB by site-directed spin labeling: insight into the mechanism of ligand transfer during protein export.

    abstract：:Export of protein into the periplasm of Escherichia coli via the general secretory system is achieved by action of a ternary complex comprising the polypeptide ligand, the chaperone SecB and SecA, a peripheral component of the membrane translocon, which is itself an ATPase. The unfolded ligand is captured initially by...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.08.022

    authors： Crane JM,Mao C,Lilly AA,Smith VF,Suo Y,Hubbell WL,Randall LL

    更新日期：2005-10-21 00:00:00

  • GTP-dependent conformational changes associated with the functional switch between Galpha and cross-linking activities in brain-derived tissue transglutaminase.

    abstract：:GTP and Ca2+, two well-known modulators of intracellular signaling pathways, control a structural/functional switch between two vital and mutually exclusive activities, cross-linking and Galpha activity, in the same enzyme. The enzyme, a brain-derived tissue-type transglutaminase (TGase), was recently cloned by us in ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2052

    authors： Monsonego A,Friedmann I,Shani Y,Eisenstein M,Schwartz M

    更新日期：1998-10-02 00:00:00

  • Thermodynamic and structural basis for transition-state stabilization in antibody-catalyzed hydrolysis.

    abstract：:Catalytic antibodies 6D9 and 9C10, which were induced by immunization with a haptenic transition-state analog (TSA), catalyze the hydrolysis of a nonbioactive chloramphenicol monoester derivative to generate a bioactive chloramphenicol. These antibodies stabilize the transition state to catalyze the hydrolysis reactio...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.03.023

    authors： Oda M,Ito N,Tsumuraya T,Suzuki K,Sakakura M,Fujii I

    更新日期：2007-05-25 00:00:00

  • Crystal structure of the Ly49I natural killer cell receptor reveals variability in dimerization mode within the Ly49 family.

    abstract：:Natural killer (NK) cells play a crucial role in the detection and destruction of virally infected and tumor cells during innate immune responses. The cytolytic activity of NK cells is regulated through a balance of inhibitory and stimulatory signals delivered by NK receptors that recognize classical major histocompat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00498-9

    authors： Dimasi N,Sawicki MW,Reineck LA,Li Y,Natarajan K,Margulies DH,Mariuzza RA

    更新日期：2002-07-12 00:00:00

  • Identifying interacting regions in the beta subunit of Escherichia coli RNA polymerase.

    abstract：:Numerous physical and genetic approaches have identified residues in the alpha, beta, beta' and sigma subunits of Escherichia coli RNA polymerase that are involved in transcriptional processes; in contrast, relatively little data exist to demonstrate interacting regions within or between the subunits themselves. As a ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0244

    authors： Tavormina PL,Reznikoff WS,Gross CA

    更新日期：1996-05-03 00:00:00

  • Characterization and mutational analysis of the RecQ core of the bloom syndrome protein.

    abstract：:Bloom syndrome protein forms an oligomeric ring structure and belongs to a group of DNA helicases showing extensive homology to the Escherichia coli DNA helicase RecQ, a suppressor of illegitimate recombination. After over-production in E.coli, we have purified the RecQ core of BLM consisting of the DEAH, RecQ-Ct and ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00534-5

    authors： Janscak P,Garcia PL,Hamburger F,Makuta Y,Shiraishi K,Imai Y,Ikeda H,Bickle TA

    更新日期：2003-06-27 00:00:00

  • Sequence-specific DNA binding of the phage Mu C protein: footprinting analysis reveals altered DNA conformation upon protein binding.

    abstract：:The mom gene of bacteriophage Mu, which codes for a DNA modification function, is regulated in a complex manner at both transcriptional and translational levels. The phage-encoded C protein functions as an activator of mom transcription. The mom promoter has features of an activator-dependent weak promoter, and the C ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0379

    authors： Ramesh V,Nagaraja V

    更新日期：1996-07-05 00:00:00

  • The crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase II from Sulfolobus solfataricus, a thermophilic enzyme stabilized by intramolecular disulfide bonds.

    abstract：:The crystal structure of Sulfolobus solfataricus 5'-deoxy-5'-methylthioadenosine phosphorylase II (SsMTAPII) in complex with 5'-deoxy-5'-methylthioadenosine (MTA) and sulfate was determined to 1.45A resolution. The hexameric structure of SsMTAPII is a dimer-of-trimers with one active site per monomer. The oligomeric a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.12.040

    authors： Zhang Y,Porcelli M,Cacciapuoti G,Ealick SE

    更新日期：2006-03-17 00:00:00

  • Crystal structure of glycosyltrehalose trehalohydrolase from the hyperthermophilic archaeum Sulfolobus solfataricus.

    abstract：:The crystal structure of glycosyltrehalose trehalohydrolase from the hyperthermophilic archaeum Sulfolobus solfataricus KM1 has been solved by multiple isomorphous replacement. The enzyme is an alpha-amylase (family 13) with unique exo-amylolytic activity for glycosyltrehalosides. It cleaves the alpha-1,4 glycosidic b...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3977

    authors： Feese MD,Kato Y,Tamada T,Kato M,Komeda T,Miura Y,Hirose M,Hondo K,Kobayashi K,Kuroki R

    更新日期：2000-08-11 00:00:00

  • Folding amphipathic helices into membranes: amphiphilicity trumps hydrophobicity.

    abstract：:High amphiphilicity is a hallmark of interfacial helices in membrane proteins and membrane-active peptides, such as toxins and antimicrobial peptides. Although there is general agreement that amphiphilicity is important for membrane-interface binding, an unanswered question is its importance relative to simple hydroph...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.05.016

    authors： Fernández-Vidal M,Jayasinghe S,Ladokhin AS,White SH

    更新日期：2007-07-13 00:00:00

  • Superhelical DNA with local substructures. A generalization of the topological constraint in terms of the intersection number and the ladder-like correspondence surface.

    abstract：:The presence of certain local structural elements in superhelical DNA, such as cruciforms and denatured loops, complicates the topological and geometric analysis of these molecules. In particular, the duplex axis is often difficult to define. In consequence, the usual conservation condition, Lk = Tw + Wr, is often ina...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90337-8

    authors： White JH,Bauer WR

    更新日期：1987-05-05 00:00:00

  • The 1.3 A crystal structure of a biotin-binding pseudoknot and the basis for RNA molecular recognition.

    abstract：:A pseudoknot-containing aptamer isolated from a pool of random sequence molecules has been shown previously to represent an optimal RNA solution to the problem of binding biotin. The affinity of this RNA molecule is nonetheless orders of magnitude weaker than that of its highly evolved protein analogs, avidin and stre...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3539

    authors： Nix J,Sussman D,Wilson C

    更新日期：2000-03-10 00:00:00

  • Point mutations that lock Salmonella typhimurium flagellar filaments in the straight right-handed and left-handed forms and their relation to filament superhelicity.

    abstract：:We have determined the nucleotide sequence of two mutant and the parent fliC genes, encoding the protein flagellin (serotype i), of Salmonella typhimurium. The flagellar filaments of the two mutants, SJW1655 and SJW1660, are locked in the straight-right-handed (R) and straight-left-handed (L) conformations, respective...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90382-g

    authors： Hyman HC,Trachtenberg S

    更新日期：1991-07-05 00:00:00

  • X-ray diffraction studies on oriented gels of vertebrate smooth muscle thin filaments.

    abstract：:The ATPase activity of acto-myosin subfragment 1 (S1) at low ratios of S1 to actin in the presence of tropomyosin is dependent on the tropomyosin source and ionic conditions. Whereas skeletal muscle tropomyosin causes a 60% inhibitory effect at all ionic strengths, the effect of smooth muscle tropomyosin was found to ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90576-6

    authors： Popp D,Holmes KC

    更新日期：1992-03-05 00:00:00

  • Prolonged Ca2+ and force transients in myosin RLC transgenic mouse fibers expressing malignant and benign FHC mutations.

    abstract：:Clinical studies have revealed that mutations in the ventricular myosin regulatory light chain (RLC) lead to the development of familial hypertrophic cardiomyopathy (FHC), an autosomal dominant disease characterized by left ventricular hypertrophy, myofibrillar disarray and sudden cardiac death. While mutations in oth...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.06.018

    authors： Wang Y,Xu Y,Kerrick WG,Wang Y,Guzman G,Diaz-Perez Z,Szczesna-Cordary D

    更新日期：2006-08-11 00:00:00

  • Computational method for the design of enzymes with altered substrate specificity.

    abstract：:A combination of enzyme kinetics and X-ray crystallographic analysis of site-specific mutants has been used to probe the determinants of substrate specificity for the enzyme alpha-lytic protease. We now present a generalized model for understanding the effects of mutagenesis on enzyme substrate specificity. This algor...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90026-3

    authors： Wilson C,Mace JE,Agard DA

    更新日期：1991-07-20 00:00:00

  • Protein structure comparison by alignment of distance matrices.

    abstract：:With a rapidly growing pool of known tertiary structures, the importance of protein structure comparison parallels that of sequence alignment. We have developed a novel algorithm (DALI) for optimal pairwise alignment of protein structures. The three-dimensional co-ordinates of each protein are used to calculate residu...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1489

    authors： Holm L,Sander C

    更新日期：1993-09-05 00:00:00

  • Gene conversion in transposition of Escherichia coli element IS30.

    abstract：:The mobile element IS30 has dual target specificity, since it can integrate adjacent to the inverted repeat (IR) of another IS30 copy or into hot-spot sequences characterized by a well-defined consensus showing no similarity to the ends of the element. The result of such integrations into these targets is different, a...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2003.10.011

    authors： Olasz F,Fischer T,Szabó M,Nagy Z,Kiss J

    更新日期：2003-12-12 00:00:00

  • 3D gene of foot-and-mouth disease virus. Conservation by convergence of average sequences.

    abstract：:The nucleotide sequence of the 3D (polymerase) gene of eight epidemiologically related isolates of foot-and-mouth disease virus of serotype C1 is reported. The genetic heterogeneity of 3D RNA is compared with that of the VP1-coding RNA of the same viruses. Regression lines of substitutions per nucleotide that distingu...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90367-1

    authors： Villaverde A,Martínez-Salas E,Domingo E

    更新日期：1988-12-05 00:00:00

  • Ligand-induced stabilization of PPARgamma monitored by NMR spectroscopy: implications for nuclear receptor activation.

    abstract：:Nuclear receptors are ligand-dependent transcription factors that are mediators of the action of lipophilic hormones and other endogenous ligands and are the targets of drugs useful in a variety of therapeutic areas. Peroxisome proliferator-activated receptor (PPAR)gamma is a nuclear receptor that, acting as a heterod...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3636

    authors： Johnson BA,Wilson EM,Li Y,Moller DE,Smith RG,Zhou G

    更新日期：2000-04-28 00:00:00

  • Structural determinants of rotavirus subgroup specificity mapped by cryo-electron microscopy.

    abstract：:The rotavirus double-layered particle (DLP) is a molecular machine that transcribes 11 genomic segments of double-stranded RNA into full-length mRNA segments during viral replication. DLPs from the human Wa strain of virus, belonging to subgroup II (SG II), possess a significantly reduced level of transcriptase activi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.11.049

    authors： Greig SL,Berriman JA,O'Brien JA,Taylor JA,Bellamy AR,Yeager MJ,Mitra AK

    更新日期：2006-02-10 00:00:00